...Kendra R. Vann; Yashavantha L. Vishweshwaraiah; Nikolay V. Dokholyan; Tatiana G. Kutateladze Methylation of lysine residues plays crucial roles in a wide variety of cell signaling processes. While the biological importance of recognition of methylated histones by reader domains in the cell nucleus...

... Protein (HP1) 1 family are key players in chromatin organisation, acting as docking sites for chromatin modifiers. Here, we inactivated HP1α in HepG2 human liver carcinoma cells and showed that HP1α participated in cell proliferation. HP1α-depleted cells have a global decrease in DNA methylation...

...Chris Nevitt; John G. Tooley; Christine E. Schaner Tooley Deciphering the histone code has illustrated that acetylation or methylation on the same residue can have analogous or opposing roles. However, little is known about the interplay between these post-translational modifications (PTMs...

... , S. , Kavak , E. , Gregory , M. , Imashimizu , M. , Shutinoski , B. , Kashlev , M. et al. ( 2011 ) CTCF-promoted RNA polymerase II pausing links DNA methylation to splicing . Nature 479 , 74 – 79 doi: 10.1038/nature10442 26 Schor , I.E. , Fiszbein...

...Martin G. Thomas; Davide Sartini; Monica Emanuelli; Matthijs J. van Haren; Nathaniel I. Martin; David M. Mountford; David J. Barlow; Fábio Klamt; David B. Ramsden; Madeehah Reza; Richard B. Parsons Nicotinamide N -methyltransferase (NNMT) is responsible for the N-methylation of nicotinamide to 1...

... transcriptional repression remains unclear. Here, we describe a functional axis involving GFI1, SMYD2, and LSD1 that is a critical contributor to GFI1-mediated transcriptional repression. SMYD2 methylates lysine-8 (K8) within a - 8 KSKK 11 - motif embedded in the GFI1 SNAG domain. Methylation-defective GFI1 SNAG...

... lysine residue modifications associated with normal tau function, soluble tau proteins isolated from four cognitively normal human brains were characterized by MS methods. The major detectable lysine modification was found to be methylation, which appeared in the form of mono- and di-methyl lysine...

... of methyl-lysine caging by calixarenes and suggests a high potential for these compounds in biochemical applications. Immunofluorescence analysis reveals that the supramolecular agents induce changes in chromatin organization that are consistent with their binding to and disruption of H3K9me3 sites...

...Janusz J. Petkowski; Lindsay A. Bonsignore; John G. Tooley; Daniel W. Wilkey; Michael L. Merchant; Ian G. Macara; Christine E. Schaner Tooley NRMT (N-terminal regulator of chromatin condensation 1 methyltransferase) was the first eukaryotic methyltransferase identified to specifically methylate...

... was induced in cell culture treated with the demethylating agent decitabine. The specific methylation of this CpG site impaired both the binding of USF and the formation of the functional NF-Y–USF complex as well as promoter activity, suggesting its importance for cell-specific transcription. Thus CpG...

...-containing and -deficient cells. From the present study we conclude that UHRF1 links epigenetic regulation with DNA replication. apoptosis caspase cell cycle methylation phosphorylation ubiquitin-like protein containing PHD and RING finger domains 1 (UHRF1) Following genotoxic injury...

...) and the other implying that short RNA forms a heteroduplex with DNA from the unwound double helix, an R-loop structure (RNA–DNA model). Likewise, the requirement for DNA methylation to enact TGS is still controversial. In vitro assays using purified recombinant murine Dnmt (DNA methyltransferase) 1-dN (where dN...

... by acetylation or methylation of Lys 9 (H3K9ac and H3K9me respectively) but is inhibited by methylation of Lys 4 (H3K4me) or acetylation of Ala 1 (H3A1ac). An 18 μM binding affinity toward unmodified H3 rises to 0.6 μM for H3K9ac and to 0.9 μM for H3K9me3, whereas it drops to 2.0 mM for H3K4me3, as measured...

... residues were detected to be methylated in PIP2 aquaporins. Lys 3 and Glu 6 of PIP2;1, one of the most abundant aquaporins in the PM, occurred as di- and mono-methylated residues respectively. Ectopic expression in Arabidopsis suspension cells of PIP2;1, either wild-type or with altered methylation sites...

...Shivakumara Bheemanaik; Yeturu V. R. Reddy; Desirazu N. Rao DNA MTases (methyltransferases) catalyse the transfer of methyl groups to DNA from AdoMet ( S -adenosyl- L -methionine) producing AdoHcy ( S -adenosyl- L -homocysteine) and methylated DNA. The C 5 and N 4 positions of cytosine and N 6...

...Mark SHEPHERD; C. Neil HUNTER Magnesium protoporphyrin IX methyltransferase (ChlM), an enzyme in the chlorophyll biosynthetic pathway, catalyses the transfer of a methyl group to magnesium protoporphyrin IX (MgP) to form magnesium protoporphyrin IX monomethyl ester (MgPME). S -Adenosyl- L...

... 2003 21 1 2004 28 1 2004 28 1 2004 The Biochemical Society, London ©2004 2004 methylation methylesterase phosphorylation phosphotyrosyl phosphatase activator protein phosphatase 2A (PP2A) signal transduction Abbreviations used: AdoMet, S -adenosylmethionine...

...Mark SHEPHERD; James D. REID; C. Neil HUNTER Magnesium protoporphyrin IX methyltransferase (ChlM), catalyses the methylation of magnesium protoporphyrin IX (MgP) at the C 6 propionate side chain to form magnesium protoporphyrin IX monomethylester (MgPME). Threading methods biased by sequence...

...2003 2003 chaperone Escherichia coli initiation of replication methylation protease sequestration Abbreviations used: LB, Luria—Bertani. Biochem. J. (2003) 370, 867 871 (Printed in Great Britain) 867 Degradation of mutant initiator protein DnaA204 by proteases ClpP, ClpQ...

... cytosine methylation and chromatin remodelling, result in alterations in gene expression which, in turn, affects the phenotype of the organism. Recent evidence, from our work and that of others in mice, suggests that these epigenetic modifications, which in the past were thought to be cleared and reset...

... determine the substrate specificity, (sub)cellular localization and catalytic activity of the PP2A holoenzymes. Moreover, the catalytic subunit is subject to two types of post-translational modification, phosphorylation and methylation, which are also thought to be important regulatory devices...