... potential of a series of complexes: [Cu(II)(BMPA)Cl 2 ] ( 1) , [Fe(III)(BMPA)Cl 3 ] ( 2) , and [Cl(BMPA)MnII-(μ-Cl)2-MnII(BMPA)-(μ-Cl)- MnII(BMPA)(Cl) 2 ]•5H 2 O ( 3) , all synthesized with the ligand bis-(2-pyridylmethyl)amine (BMPA) in Saccharomyces cerevisiae exposed to G-iS caused by methylglyoxal (MG...

... and the Biochemical Society under a transformative agreement with Individual. acetylation/deacetylation acylation glycation mass spectrometry methylglyoxal post-translational modification The ability of cells to rapidly detect and respond to stimuli is critical to maintaining homeostasis [ 1 , 2...

...Su-Ping Ng; Wataru Nomura; Haruya Takahashi; Kazuo Inoue; Teruo Kawada; Tsuyoshi Goto; Yoshiharu Inoue Certain metabolic intermediates produced during metabolism are known to regulate a wide range of cellular processes. Methylglyoxal (MG), a natural metabolite derived from glycolysis, has been...

... study, we show that DHA toxicity is due to its spontaneous conversion to methylglyoxal (MG) within yeast cells. A mutant defective in MG-metabolizing enzymes ( glo1 Δ gre2 Δ gre3 Δ) exhibited higher susceptibility to DHA. Intracellular MG levels increased following the treatment of glo1 Δ gre2 Δ gre3 Δ...

... nitro-drugs such as PA-824. Rather, these enzymes metabolise a variety of toxic ketoaldehydes, such as glyoxal and methylglyoxal, suggesting a role in cellular defence against chemical stress. UPLC-QToF/MS analysis of benznidazole bioactivation by T. cruzi cell lysates confirms previous reports...

... are glycated by methylglyoxal. Even though no consensus has been reached about the actual role of methylglyoxal-derived advanced glycation end-products in amyloid diseases, evidence collected so far points to a role for protein glycation in conformational abnormalities, being ubiquitously found in amyloid...

...Marta Sousa Silva; Ricardo A. Gomes; Antonio E. N. Ferreira; Ana Ponces Freire; Carlos Cordeiro The discovery of the enzymatic formation of lactic acid from methylglyoxal dates back to 1913 and was believed to be associated with one enzyme termed ketonaldehydemutase or glyoxalase, the latter...

...Mingzhan Xue; Naila Rabbani; Hiroshi Momiji; Precious Imbasi; M. Maqsud Anwar; Neil Kitteringham; B. Kevin Park; Tomokazu Souma; Takashi Moriguchi; Masayuki Yamamoto; Paul J. Thornalley Abnormal cellular accumulation of the dicarbonyl metabolite MG (methylglyoxal) occurs on exposure to high glucose...

...Yoshifumi Takatsume; Takumi Ohdate; Kazuhiro Maeta; Wataru Nomura; Shingo Izawa; Yoshiharu Inoue Although methylglyoxal is derived from glycolysis, it has adverse effects on cellular function. Hence, the intrinsic role of methylglyoxal in vivo remains to be determined. Glyoxalase 1 is a pivotal...

...-state of glycation agents, namely methylglyoxal, whereas in vitro , a bolus of a suitable glycation agent is added to diluted protein samples. In the present study, yeast was shown to be an ideal model to investigate glycation in vivo since it shows different glycation phenotypes and presents specific...

... glycation end-products) in various tissues, including the lens. Nevertheless, glycation of α-crystallin with various sugars has resulted in divergent results. In the present in vitro study, we have investigated the effect of glucose, fructose, G6P (glucose 6-phosphate) and MGO (methylglyoxal), which...

... should be addressed (email [email protected] ). 19 3 2007 12 6 2007 19 6 2007 19 6 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 cell death glyoxalase I (GLO1) methylglyoxal nitrosative stress post-translational modification...

...-lysine and the methylglyoxal-derived products, carboxyethyl-lysine, argpyrimidine and MODIC (methylglyoxal-derived imidazolium cross-link). These results provide support for the presence of metal-catalysed oxidation (the Suyama pathway) in diabetes and the possible activation of myeloperoxidase during...

...Neil Greig; Susan Wyllie; Tim J. Vickers; Alan H. Fairlamb The glyoxalase system, comprizing glyoxalase I and glyoxalase II, is a ubiquitous pathway that detoxifies highly reactive aldehydes, such as methylglyoxal, using glutathione as a cofactor. Recent studies of Leishmania major glyoxalase I...

... proteins. It was shown recently that glycation causes the structural transition from the folded soluble form to β-fibrils in serum albumin. We identified for the first time a methylglyoxal-derived advanced glycation end-product, argpyrimidine [ N δ -(5-hydroxy-4,6-dimethylpyrimidin-2-yl)- L -ornithine...

... various stress conditions. Non-enzymic glycation of long-lived proteins has been implicated in several age- and diabetes-related complications, including cataract. Dicarbonyl compounds such as methylglyoxal and glyoxal have been identified as the predominant source for the formation of advanced glycation...

... correspondence should be addressed (e-mail [email protected] ). 23 5 2003 15 7 2003 29 7 2003 29 7 2003 The Biochemical Society, London ©2003 2003 diabetes fructosamine methylglyoxal 3-nitrotyrosine oxidative stress uraemia Abbreviations used: AGE, advanced...

...Soumya Sinha ROY; Swati BISWAS; Manju RAY; Subhankar RAY Previous publications from our laboratory have shown that methylglyoxal inhibits mitochondrial respiration of malignant and cardiac cells, but it has no effect on mitochondrial respiration of other normal cells [Biswas, Ray, Misra, Dutta...

... and methylglyoxal, all of which are pertinent to cataract. Also modification by aspirin, which is known to delay cataract and other diseases, has been investigated. Recently, two point mutations of arginine residues were shown to cause congenital cataract. 1,2-Cyclohexanedione modifies arginine residues...

... isomers of methylglyoxal-derived hydroimidazolone, glyoxal-derived hydroimidazolone, 3-deoxyglucosone-derived hydroimidazolone and N δ -(4-carboxy-4,6-dimethyl-5,6-dihydroxy-1,4,5,6-tetrahydropyrimidin-2-yl)-ornithine (THP) were determined for the first time. AGEs with intrinsic fluorescence...

...Naila AHMED; Paul J. THORNALLEY Glycation of proteins leads to the formation of advanced glycation endproducts (AGEs) of diverse molecular structure and biological function. Serum albumin derivatives modified to minimal and high extents by methylglyoxal and glucose in vitro have been used in many...