..., that can be used for the treatment of COVID-19. To identify additional potential therapeutics, we investigated the enzymatic proteins encoded in the SARS-CoV-2 genome. In this study, we focussed on the viral RNA cap methyltransferases, which play key roles in enabling viral protein translation...

...Pål Ø. Falnes; Magnus E. Jakobsson; Erna Davydova; Angela Ho; Jędrzej Małecki Methylation of biomolecules is a frequent biochemical reaction within the cell, and a plethora of highly specific methyltransferases (MTases) catalyse the transfer of a methyl group from S -adenosylmethionine (AdoMet...

...Janusz J. Petkowski; Lindsay A. Bonsignore; John G. Tooley; Daniel W. Wilkey; Michael L. Merchant; Ian G. Macara; Christine E. Schaner Tooley NRMT (N-terminal regulator of chromatin condensation 1 methyltransferase) was the first eukaryotic methyltransferase identified to specifically methylate...

...Alexandra Plotnikova; Simona Baranauskė; Aleksandr Osipenko; Saulius Klimašauskas; Giedrius Vilkaitis The HEN1 methyltransferase from Arabidopsis thaliana modifies the 3′-terminal nucleotides of small regulatory RNAs. Although it is one of the best characterized members of the 2′- O...

... it is only active in the context of the multi-subunit PRC2 (polycomb repressive complex 2). vSET is a viral lysine methyltransferase that represents the smallest protein unit capable of catalysing H3K27 methylation. The crystal structure of this minimal catalytic protein has been solved and researchers have...

... 2011 18 1 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 chalcogen, detoxification methyltransferase nucleophilic attack selenium oxyanion tellurite oxyanion The chalcogens selenium and tellurium can be found as trace elements in the environment...

...-functional R-M systems or R-M systems with new properties. hpyAVIAM and hpyAVIBM are two solitary DNA MTase (methyltransferase) genes adjacent to each other and lacking a cognate restriction enzyme gene in H. pylori strain 26695. Interestingly, in an Indian strain D27, hpyAVIAM – hpyAVIBM encodes a single...

...Artur Sawicki; Robert D. Willows The enzyme BchM ( S -adenosyl- L -methionine:magnesium-protoporphyrin IX O -methyltransferase) from Rhodobacter capsulatus catalyses an intermediate reaction in the bacteriochlorophyll biosynthetic pathway. Overexpression of His 6 -tagged protein in Escherichia coli...

... the identification of biochemical targets not found in host organisms. Recent studies suggest that Caenorhabditis elegans synthesizes phosphocholine through the action of PEAMT ( S -adenosyl- L -methionine:phosphoethanolamine N -methyltransferases) that convert phosphoethanolamine into phosphocholine. Here, we...

... arginine methyltransferases that catalyses the formation of ω- N G -monomethylarginine and symmetric ω- N G , N ′ G -dimethylarginine residues in a number of methyl-accepting species. A full-length HSL7 construct was expressed as a FLAG-tagged protein in Saccharomyces cerevisiae . We found that FLAG-tagged...

...Marie-Chloé BOULANGER; Tina Branscombe MIRANDA; Steven CLARKE; Marco di FRUSCIO; Beat SUTER; Paul LASKO; Stéphane RICHARD The role of arginine methylation in Drosophila melanogaster is unknown. We identified a family of nine PRMTs (protein arginine methyltransferases) by sequence homology...

...Sriharsa PRADHAN; Nigel A. R. URWIN; Gareth I. JENKINS; Roger L. P. ADAMS The presence of two DNA methyltransferases in Pisum raises the possibility that they serve different functions. In vitro methylation of CWG sequences in the strong cauliflower mosaic virus 35S promoter had no effect...

...Jennifer A. COBB; Chang-Hoon HAN; David M. WILLS; Daniel M. ROBERTS Calmodulin is trimethylated by a specific methyltransferase on Lys 115 , a residue located in a six amino acid loop (LGEKLT) between EF hands III and IV. To investigate the structural requirements for methylation, domain exchange...