... of 2′-5′-oligoadenylate synthase 2 (OAS2) and interferon-induced protein with tetratricopeptide repeats 1 (Ifit1) correlated with the kinetics of STAT2 phosphorylation, and the presence of a STAT2/IRF9 complex requiring STAT2 phosphorylation and the STAT2 transactivation domain. Subsequent microarray...

.... To identify Atf3-regulated genes, we knocked down Atf3 expression in cardiomyocytes exposed to endothelin-1 and used microarrays to interrogate the transcriptomic effects. The expression of 23 mRNAs (including Egr1 and Ptgs2) was enhanced and the expression of 25 mRNAs was inhibited by Atf3 knockdown. Using...

... or how their expression is altered in diseased states is sparse. To facilitate these studies we have developed a snoRNA microarray platform for the analysis of the abundance of snoRNAs in different RNA samples. In the present study we show that the microarray is sensitive and specific for the detection...

... correspondence should be addressed (email [email protected] ). 30 1 2008 13 3 2008 28 3 2008 28 3 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 gene expression liver microarray selenocysteine (Sec) tRNA Trsp knockout xenobiotic...

... demonstrated that indeed PGXB and PGXC act as exo-polygalacturonases, whereas PGXA acts as an exo-xylogalacturonan hydrolase. The expression levels of all 21 genes were assessed by microarray analysis. The results from the present study demonstrate that exo-acting glycoside hydrolases play a prominent role...

...Hong Soon Kang; Ju Youn Beak; Yong-Sik Kim; Robert M. Petrovich; Jennifer B. Collins; Sherry F. Grissom; Anton M. Jetten RORγ2 (retinoid-related orphan receptor γ2) plays a critical role in the regulation of thymopoiesis. Microarray analysis was performed in order to uncover differences in gene...

...Jennifer R. Mackley; Joji Ando; Pawel Herzyk; Steven J. Winder Primary fibroblasts isolated from foetal mouse cornea, skin and tendon were subjected to linear shear stress and analysed for morphological parameters and by microarray, as compared with unstimulated controls. Approx. 350 genes were...

[email protected] ). 19 2 2004 17 8 2004 23 8 2004 22 11 2004 The Biochemical Society, London 2005 To investigate alterations in gene expression, microarray technology is a useful and highly specific tool. We used customdesigned cDNA microarrays, containing 793 defined...

... from each steady-state culture, labelled with Cy3 or Cy5 and hybridized to commercial oligonucleotide microarrays (Agilent Technologies) representing 6256 S. cerevisiae ORFs. The resulting data set is shown in Table S1 (available at http://www.BiochemJ.org/bj/382/bj3820867add.htm ), and is deposited...

..., hybridizations and scaling of raw data were performed by Expression Analysis Inc. (Durham, NC, U.S.A.) in accordance with Affymetrix protocols. Data were analysed using Microarray Suite 5.0. The amount of starting RNA was determined by absorption spectrophotometry, and the quality was confirmed using...

... (Santa Clara, CA, U.S.A.) oligonucleotide microarray analysis, we found that TGIF mRNA level was elevated by transforming-growth-factor-β (TGF-β) treatment in a human T-cell line, HuT78. Subsequent reverse-transcription PCR assays indicated that TGF-β1 and activin were able to induce a rapid...

....) 101 , 249–258]. In the present study, we conducted microarray analysis of HeLa cells, although our analysis covered only a small fraction of the human genome. A great majority of human ER stress-inducible genes (approx. 1% of 1800 genes examined) were classified into two groups. One group consisted...