...Mohammed M. Alhadidy; Rebecca L. Mueller; Jared Lamp; Nicholas M. Kanaan Tau is subject to a broad range of post-translational modifications (PTMs) that regulate its biological activity in health and disease, including microtubule (MT) dynamics, aggregation, and adoption of pathogenic conformations...

...Shweta Shyam Prassanawar; Dulal Panda Microtubules, composed of αβ-tubulin heterodimers, exhibit diverse structural and functional properties in different cell types. The diversity in the microtubule structure originates from tubulin heterogeneities, namely tubulin isotypes and their post...

... acetylation and ablated the dynamic behaviour of microtubules in live cells. siRNA-mediated knockdown (KD) of HDAC3 in PC3 cells, significantly increased levels of tubulin acetylation, and overexpression reduced it. However, the active HDAC3–silencing mediator of retinoic and thyroid receptors (SMRT...

... (acetylated tubulin) and (for comparison) preparations lacking AcTubulin. Assembly–disassembly cycling of microtubules gives tubulin preparations that contain little or no AcTubulin. In the present study we demonstrated that this result is owing to the presence of high deacetylating activity in the extracts...

... the microtubule-associated colonic and hepatic tumour overexpressed (ch-TOG) protein, and the oncogenic transcription factor glioma amplified sequence 41/NuMA binding protein 1 (GAS41/NuBI1; where NuMA stands for nuclear mitotic apparatus protein 1). This suggests that the TACC proteins can form multiple...

...Francisco RAMOS-MORALES; Carmen VIME; Michel BORNENS; Concepción FEDRIANI; Rosa M. RIOS GMAP-210 (Golgi-microtubule-associated protein of 210kDa) is a peripheral Golgi protein that interacts with the minus end of microtubules through its C-terminus and with cis -Golgi network membranes through its...

... by protein sequencing. In addition, not only the β isoform of tubulin, but also the α and γ isoforms, were detected in the protein absorbed from cell lysates with GST-p55γN 1-34 and GST-p55αN 1-34 by immunoblotting. Indeed, the only regulatory subunit present in the purified microtubule assembly from rat...