Page 1 | Search Results | Biochemical Journal

1-50 of 197

Sort by

Sort Order Select

Articles

Biochemical basis and therapeutic potential of mitochondrial uncoupling in cardiometabolic syndrome

Bernardo Gindri dos Santos, Niki F. Brisnovali, Leigh Goedeke

Biochem J (2024) 481 (23): 1831–1854.

https://doi.org/10.1042/BCJ20240005

Published: 04 December 2024

...Bernardo Gindri dos Santos; Niki F. Brisnovali; Leigh Goedeke Mild uncoupling of oxidative phosphorylation is an intrinsic property of all mitochondria, allowing for adjustments in cellular energy metabolism to maintain metabolic homeostasis. Small molecule uncouplers have been extensively studied...

View article titled, Biochemical basis and therapeutic potential of mitochondrial uncoupling in cardiometabolic syndrome

Articles

The pro-drug C13 activates AMPK by two distinct mechanisms

Jordana B. Freemantle, Dinesh Shah, Dylan M. Lynch, Alessio Ciulli, Harinder S. Hundal, D. Grahame Hardie

Biochem J (2024) 481 (18): 1203–1219.

https://doi.org/10.1042/BCJ20240425

Published: 12 September 2024

... and the Biochemical Society under a transformative agreement with JISC. AMPK C13 formaldehyde mitochondria mitochondrial respiration AMP-activated protein kinase (AMPK) is the key component of a signalling pathway that is activated either by deprivation of cellular nutrients (especially glucose [ 1...

View article titled, The pro-drug C13 activates AMPK by two distinct mechanisms

Includes: Supplementary data

Articles

Validation of GCN5L1/BLOC1S1/BLOS1 antibodies using knockout cells and tissue

Paramesha Bugga, Michael W. Stoner, Janet R. Manning, Bellina A.S. Mushala, Nisha Bhattarai, Maryam Sharifi-Sanjani, Bradley R. Webster, Dharendra Thapa, Iain Scott

Biochem J (2024) 481 (10): 643–651.

https://doi.org/10.1042/BCJ20230302

Published: 10 May 2024

... Thapa ( [email protected] ) * These authors contributed equally to this work. 24 7 2023 18 4 2024 29 4 2024 © 2024 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2024 antibodies lysosomes mitochondria validation...

View article titled, Validation of GCN5L1/BLOC1S1/BLOS1 antibodies using knockout cells and tissue

Includes: Supplementary data

Articles

Cardiomyocyte-specific deletion of GCN5L1 reduces lysine acetylation and attenuates diastolic dysfunction in aged mice by improving cardiac fatty acid oxidation

Jackson E. Stewart, Jenna M. Crawford, William E. Mullen, Angelica Jacques, Michael W. Stoner, Iain Scott, Dharendra Thapa

Biochem J (2024) 481 (6): 423–436.

https://doi.org/10.1042/BCJ20230421

Published: 11 March 2024

... in the corresponding deacetylase, total lysine acetylation was measured by utilizing a pan acetyl lysine antibody and all bands in individual lanes were quantified to capture overall mitochondrial protein acetylation. As reported, overall acetylation in the aged mouse heart mitochondria was significantly elevated when...

View article titled, Cardiomyocyte-specific deletion of GCN5L1 reduces lysine acetylation and attenuates diastolic dysfunction in aged mice by improving cardiac fatty acid oxidation

Articles

Colonic ketogenesis, a microbiota-regulated process, contributes to blood ketones and protects against colitis in mice

Kevin Bass, Sathish Sivaprakasam, Gunadharini Dharmalingam-Nandagopal, Muthusamy Thangaraju, Vadivel Ganapathy

Biochem J (2024) 481 (4): 295–312.

https://doi.org/10.1042/BCJ20230403

Published: 19 February 2024

... synthase-2 inflammatory bowel disease inflammatory cytokines microbiome mitochondria Ketone bodies (acetoacetate, β-hydroxybutyrate) are generated via ketogenesis in mitochondria using acetyl-CoA and acetoacetyl-CoA produced by β-oxidation of fatty acids [ 1 , 2 ]. This metabolic pathway...

View article titled, Colonic ketogenesis, a microbiota-regulated process, contributes to blood ketones and protects against colitis in mice

Includes: Supplementary data

Articles

The consequence of ATP synthase dimer angle on mitochondrial morphology studied by cryo-electron tomography

Emma Buzzard, Mathew McLaren, Piotr Bragoszewski, Andrea Brancaccio, Holly C. Ford, Bertram Daum, Patricia Kuwabara, Ian Collinson, Vicki A.M. Gold

Biochem J (2024) 481 (3): 161–175.

https://doi.org/10.1042/BCJ20230450

Published: 29 January 2024

... subunits are present in the mature proteins and corroborate mitochondrial targeting sequence predictions ( Supplementary Figure S5 ). alphafold ATP synthase cryo-electron tomography mitochondria sub-tomogram averaging The F 1 F o ATP synthase is a molecular motor ubiquitous to all living...

View article titled, The consequence of ATP synthase dimer angle on mitochondrial morphology studied by cryo-electron tomography

Includes: Multimedia, Supplementary data

Articles

The role of mitochondrial RNA association for mitochondrial homeostasis in neurons

Inmaculada Segura, Angelika Harbauer

Biochem J (2024) 481 (3): 119–139.

https://doi.org/10.1042/BCJ20230110

Published: 29 January 2024

... to humans and it regulates several aspects of mitochondrial physiology and, hence, cellular functions. In neurons, mitochondria are emerging as platforms for RNA transport and local protein translation. In this review, we discuss how RNA localization to mitochondria helps to sustain mitochondrial function...

View article titled, The role of mitochondrial RNA association for mitochondrial homeostasis in neurons

Articles

Interactions of mitochondrial and skeletal muscle biology in mitochondrial myopathy

In Collection

Awards

Valeria Di Leo, Tiago M. Bernardino Gomes, Amy E. Vincent

Biochem J (2023) 480 (21): 1767–1789.

https://doi.org/10.1042/BCJ20220233

Published: 15 November 2023

... by the restoration to baseline levels of mitochondrial stress markers with the expansion of healthy mitochondria in the skeletal muscle of RIRCD patients [ 54 ]. Mitochondrial metabolism produces metabolites, including acetyl-CoA and α-ketoglutarate, which are involved in epigenetic changes that modulate gene...

View article titled, Interactions of mitochondrial and skeletal muscle biology in mitochondrial myopathy

Articles

Seasonal cold induces divergent structural/biochemical adaptations in different skeletal muscles of Columba livia : evidence for nonshivering thermogenesis in adult birds

Punyadhara Pani, Gourabamani Swalsingh, Sunil Pani, Unmod Senapati, Bijayashree Sahu, Benudhara Pati, Subhasmita Rout, Naresh C. Bal

Biochem J (2023) 480 (17): 1397–1409.

https://doi.org/10.1042/BCJ20230245

Published: 04 September 2023

... acclimatization futile Ca 2+ cycling mitochondria non-shivering thermogenesis skeletal muscle Skeletal muscle has long been proposed as the site of avian NST since the first study as early as 1928 in denervated pigeons [ 7 ]. Major support for muscle as the primary site of avian NST came from...

View article titled, Seasonal cold induces divergent structural/biochemical adaptations in different skeletal muscles of Columba livia : evidence for nonshivering thermogenesis in adult birds

Includes: Supplementary data

Articles

Mitochondrial metabolism and dynamics in pancreatic beta cell glucose sensing

In Collection

Awards

Guy A. Rutter, Vaibhav Sidarala, Brett A. Kaufman, Scott A. Soleimanpour

Biochem J (2023) 480 (11): 773–789.

https://doi.org/10.1042/BCJ20230167

Published: 07 June 2023

... This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . diabetes glucose homeostasis hormone secretion insulin mitochondria pancreatic beta cell Type 2 diabetes (T2D...

View article titled, Mitochondrial metabolism and dynamics in pancreatic beta cell glucose sensing

Articles

Site I Q in mitochondrial complex I generates S1QEL-sensitive superoxide/hydrogen peroxide in both the reverse and forward reactions

Edwin T. Gibbs, II, Chad A. Lerner, Mark A. Watson, Hoi-Shan Wong, Akos A. Gerencser, Martin D. Brand

Biochem J (2023) 480 (5): 363–384.

https://doi.org/10.1042/BCJ20220611

Published: 15 March 2023

..., the endogenous matrix NAD pool will become more reduced if flow before the challenge was forward, but more oxidised if flow was reverse. Using this assay we show in the model system of isolated rat skeletal muscle mitochondria that superoxide/hydrogen peroxide production by site I Q can be equally great whether...

View article titled, Site I Q in mitochondrial complex I generates S1QEL-sensitive superoxide/hydrogen peroxide in both the reverse and forward reactions

Articles

Proteomic analysis of dietary restriction in yeast reveals a role for Hsp26 in replicative lifespan extension

Richard Campion, Leanne Bloxam, Kimberley Burrow, Philip J. Brownridge, Daniel R. Pentland, Patricia Thomas, Campbell W. Gourlay, Claire E. Eyers, Jeff W. Barclay, Alan Morgan

Biochem J (2021) 478 (24): 4153–4167.

https://doi.org/10.1042/BCJ20210432

Published: 16 December 2021

... agreement with JISC. aging mitochondria molecular chaperones Saccharomyces cerevisiae Dietary restriction (DR) is the most robust form of environmental manipulation known to increase longevity in a range of organisms [ 1 ]. First discovered to extend the lifespan of laboratory rats [ 2...

View article titled, Proteomic analysis of dietary restriction in yeast reveals a role for Hsp26 in replicative lifespan extension

Includes: Supplementary data

Articles

Revisiting the contribution of mitochondrial biology to the pathophysiology of skeletal muscle insulin resistance

In Collection

Celebrating 100 years of insulin research

Sara M. Frangos, David J. Bishop, Graham P. Holloway

Biochem J (2021) 478 (21): 3809–3826.

https://doi.org/10.1042/BCJ20210145

Published: 09 November 2021

... source of ROS production. Classic studies have suggested that reductions in skeletal muscle mitochondrial content and/or function contribute to lipid-induced insulin resistance; however, in recent years the role of mitochondria in the pathophysiology of insulin resistance has been gradually re-evaluated...

View article titled, Revisiting the contribution of mitochondrial biology to the pathophysiology of skeletal muscle insulin resistance

Articles

Quality control of protein import into mitochondria

Fabian den Brave, Jeannine Engelke, Thomas Becker

Biochem J (2021) 478 (16): 3125–3143.

https://doi.org/10.1042/BCJ20190584

Published: 26 August 2021

...Fabian den Brave; Jeannine Engelke; Thomas Becker Mitochondria import about 1000 proteins that are produced as precursors on cytosolic ribosomes. Defects in mitochondrial protein import result in the accumulation of non-imported precursor proteins and proteotoxic stress. The cell is equipped...

View article titled, Quality control of protein import into mitochondria

Articles

Insulin rapidly increases skeletal muscle mitochondrial ADP sensitivity in the absence of a high lipid environment

Henver S. Brunetta, Heather L. Petrick, Bayley Vachon, Everson A. Nunes, Graham P. Holloway

Biochem J (2021) 478 (13): 2539–2553.

https://doi.org/10.1042/BCJ20210264

Published: 09 July 2021

... of intracellular lipids. insulin resistance mitochondria muscle metabolism obesity skeletal muscle Skeletal muscle plays a fundamental role in the maintenance of glucose homeostasis, and as a result, a reduction in skeletal muscle insulin responsiveness is implicated in the development of type 2...

View article titled, Insulin rapidly increases skeletal muscle mitochondrial ADP sensitivity in the absence of a high lipid environment

Articles

Oxidative signalling in seed germination and early seedling growth: an emerging role for ROS trafficking and inter-organelle communication

Christophe Bailly, Livia Merendino

Biochem J (2021) 478 (10): 1977–1984.

https://doi.org/10.1042/BCJ20200934

Published: 28 May 2021

... compartments. We point out the role of mitochondria as an environmental and developmental sensor organelle that regulates ROS homeostasis and downstream events and we propose future directions of research that should help better understanding the roles of ROS in germination and seedling emergence...

View article titled, Oxidative signalling in seed germination and early seedling growth: an emerging role for ROS trafficking and inter-organelle communication

Articles

The role of mitochondria in cocaine addiction

Claire Thornton, Etty Grad, Rami Yaka

Biochem J (2021) 478 (4): 749–764.

https://doi.org/10.1042/BCJ20200615

Published: 24 February 2021

.... In mitochondria, the production of ATP by oxidative phosphorylation depends on the efficient functioning of the electron transport chain (ETC) complexes I–IV and ATP synthase (also known as complex V) located on the inner mitochondrial membrane. The passage of electrons through the ETC results in the pumping...

View article titled, The role of mitochondria in cocaine addiction

Articles

Blackout in the powerhouse: clinical phenotypes associated with defects in the assembly of OXPHOS complexes and the mitoribosome

Daniella H. Hock, David R. L. Robinson, David A. Stroud

Biochem J (2020) 477 (21): 4085–4132.

https://doi.org/10.1042/BCJ20190767

Published: 05 November 2020

...Daniella H. Hock; David R. L. Robinson; David A. Stroud Mitochondria produce the bulk of the energy used by almost all eukaryotic cells through oxidative phosphorylation (OXPHOS) which occurs on the four complexes of the respiratory chain and the F 1 –F 0 ATPase. Mitochondrial diseases...

View article titled, Blackout in the powerhouse: clinical phenotypes associated with defects in the assembly of OXPHOS complexes and the mitoribosome

Articles

Amino acid starvation inhibits autophagy in lipid droplet-deficient cells through mitochondrial dysfunction

Pierre Voisin, Marianne Bernard, Thierry Bergès, Matthieu Régnacq

Biochem J (2020) 477 (18): 3613–3623.

https://doi.org/10.1042/BCJ20200551

Published: 28 September 2020

... is not involved in this paradoxical effect of amino acid shortage. We correlate the autophagy failure with mitochondria aggregation and we show that amino acid starvation-induced autophagy is restored in lipid droplet-deficient yeast by increasing mitochondrial biomass physiologically (respiration) or genetically...

View article titled, Amino acid starvation inhibits autophagy in lipid droplet-deficient cells through mitochondrial dysfunction

Includes: Supplementary data

Articles

Degradation of mitochondrial alternative oxidase in the appendices of Arum maculatum

Kikukatsu Ito, Takafumi Ogata, Takanari Seito, Yui Umekawa, Yusuke Kakizaki, Hiroshi Osada, Anthony L. Moore

Biochem J (2020) 477 (17): 3417–3431.

https://doi.org/10.1042/BCJ20200515

Published: 17 September 2020

... a role in elevated respiration in mitochondria, it remains unclear whether thermogenic plants possess molecular mechanisms for the mitochondrial degradation of AOX. To better understand the mechanism of AOX turnover in mitochondria, we performed a series of in organello AOX degradation assays using...

View article titled, Degradation of mitochondrial alternative oxidase in the appendices of Arum maculatum

Includes: Supplementary data

Articles

Inorganic polyphosphate is produced and hydrolyzed in F 0 F 1 -ATP synthase of mammalian mitochondria

Artyom Y. Baev, Plamena R. Angelova, Andrey Y. Abramov

Biochem J (2020) 477 (8): 1515–1524.

https://doi.org/10.1042/BCJ20200042

Published: 29 April 2020

... ). Moreover, PolyP can stimulate F 0 F 1 -ATPase activity in the presence of ATP and, importantly, can be hydrolyzed in this enzyme instead of ATP. Furthermore, PolyP can be produced in mitochondria in the presence of substrates for respiration and phosphate by the F 0 F 1 -ATPsynthase. Thus, polyP...

View article titled, Inorganic polyphosphate is produced and hydrolyzed in F 0 F 1 -ATP synthase of mammalian mitochondria

Articles

UCP1-independent thermogenesis

In Collection

Adipose biology

Anna Roesler, Lawrence Kazak

Biochem J (2020) 477 (3): 709–725.

https://doi.org/10.1042/BCJ20190463

Published: 14 February 2020

... of the Biochemical Society 2020 brown adipose tissue mitochondria obesity thermogenesis The significance of brown adipose tissue (BAT) in body weight regulation is under intense investigation. The study of thermogenic mechanisms in mammals may lay the foundation to exploit these biochemical pathways...

View article titled, UCP1-independent thermogenesis

Articles

Lack of mitochondrial NADP(H)-transhydrogenase expression in macrophages exacerbates atherosclerosis in hypercholesterolemic mice

Alessandro G. Salerno, Thiago Rentz, Gabriel G. Dorighello, Ana Carolina Marques, Estela Lorza-Gil, Amarylis C. B. A. Wanschel, Audrey de Moraes, Anibal E. Vercesi, Helena C. F. Oliveira

Biochem J (2019) 476 (24): 3769–3789.

https://doi.org/10.1042/BCJ20190543

Published: 20 December 2019

... oxidative stress and increase atherosclerosis development. Thus, we compared peritoneal macrophages and liver mitochondria from three C57BL/6J mice lines: Ldlr and Nnt double mutant, single Nnt mutant and wild-type. We found increased oxidants production in both mitochondria and macrophages according...

View article titled, Lack of mitochondrial NADP(H)-transhydrogenase expression in macrophages exacerbates atherosclerosis in hypercholesterolemic mice

Includes: Supplementary data

Articles

Mitofusins modulate the increase in mitochondrial length, bioenergetics and secretory phenotype in therapy-induced senescent melanoma cells

In Collection

Editors' pick of research highlights published in the Biochemical Journal in 2018 & 2019

Jennyfer Martínez, Doménica Tarallo, Laura Martínez-Palma, Sabina Victoria, Mariana Bresque, Sebastián Rodríguez-Bottero, Inés Marmisolle, Carlos Escande, Patricia Cassina, Gabriela Casanova, Mariela Bollati-Fogolín, Caroline Agorio, María Moreno, Celia Quijano

Biochem J (2019) 476 (17): 2463–2486.

https://doi.org/10.1042/BCJ20190405

Published: 10 September 2019

... metabolism; and highlight mitochondria as potential pharmacological targets to modulate senescence and the SASP. Correspondence: Celia Quijano ( [email protected] ; [email protected] ) 22 5 2019 8 8 2019 19 8 2019 20 8 2019 © 2019 The Author(s) 2019 This is an open...

View article titled, Mitofusins modulate the increase in mitochondrial length, bioenergetics and secretory phenotype in therapy-induced senescent melanoma cells

Includes: Supplementary data

Articles

New tools for an old question: dependence of ATP and bicarbonate for branched-chain keto acids oxidation

Henver S. Brunetta, Graham P. Holloway

Biochem J (2019) 476 (15): 2235–2237.

https://doi.org/10.1042/BCJ20190368

Published: 15 August 2019

...Henver S. Brunetta; Graham P. Holloway Branched-chain keto acids (BCKA) metabolism involves several well-regulated steps within mitochondria, requires cofactors, and is modulated according to the metabolic status of the cells. This regulation has made it challenging to utilize in vitro approaches...

View article titled, New tools for an old question: dependence of ATP and bicarbonate for branched-chain keto acids oxidation

Articles

Mechanism of nitrite-dependent NO synthesis by human sulfite oxidase

Daniel Bender, Alexander Tobias Kaczmarek, Dimitri Niks, Russ Hille, Guenter Schwarz

Biochem J (2019) 476 (12): 1805–1815.

https://doi.org/10.1042/BCJ20190143

Published: 28 June 2019

... be tuned. mitochondria molybdenum cofactor nitric oxide nitrite reduction sulfite oxidase PCR fragments of human SO cDNA (NM_000456) were cloned into pQE80L (Qiagen, Hilden, Germany) without mitochondrial targeting sequence (Δ1-79; full-length SO) using SacI and SalI as restriction...

View article titled, Mechanism of nitrite-dependent NO synthesis by human sulfite oxidase

Articles

Tissue-specific characterization of mitochondrial branched-chain keto acid oxidation using a multiplexed assay platform

Emma J. Goldberg, Katherine A. Buddo, Kelsey L. McLaughlin, Regina F. Fernandez, Andrea S. Pereyra, Christine E. Psaltis, Chien-Te Lin, James T. Hagen, Ilya N. Boykov, Tiffany K. Nguyen, Kymberly M. Gowdy, Jessica M. Ellis, P. Darrell Neufer, Joseph M. McClung, Kelsey H. Fisher-Wellman

Biochem J (2019) 476 (10): 1521–1537.

https://doi.org/10.1042/BCJ20190182

Published: 31 May 2019

... conditions, the underlying biochemical mechanism(s) within the mitochondrion remain largely unknown. In vitro experiments using isolated mitochondria represent a powerful biochemical tool for elucidating the role of the mitochondrion in driving disease. Such analyses have routinely been utilized across...

View article titled, Tissue-specific characterization of mitochondrial branched-chain keto acid oxidation using a multiplexed assay platform

Includes: Supplementary data

Articles

Strangers in strange lands: mitochondrial proteins found at extra-mitochondrial locations

David P. Scanlon, Michael W. Salter

Biochem J (2019) 476 (1): 25–37.

https://doi.org/10.1042/BCJ20180473

Published: 07 January 2019

...David P. Scanlon; Michael W. Salter The mitochondrial proteome is estimated to contain ∼1100 proteins, the vast majority of which are nuclear-encoded, with only 13 proteins encoded by the mitochondrial genome. The import of these nuclear-encoded proteins into mitochondria was widely believed...

View article titled, Strangers in strange lands: mitochondrial proteins found at extra-mitochondrial locations

Articles

Insights into leptin signaling and male reproductive health: the missing link between overweight and subfertility?

Bruno P. Moreira, Mariana P. Monteiro, Mário Sousa, Pedro F. Oliveira, Marco G. Alves

Biochem J (2018) 475 (22): 3535–3560.

https://doi.org/10.1042/BCJ20180631

Published: 20 November 2018

... signaling, mitochondria and relevant pathways for the nutritional support of spermatogenesis. 9 8 2018 28 9 2018 19 10 2018 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 leptin male fertility mitochondria obesity Sertoli...

View article titled, Insights into leptin signaling and male reproductive health: the missing link between overweight and subfertility?

Articles

Sab concentrations indicate chemotherapeutic susceptibility in ovarian cancer cell lines

Iru Paudel, Sean M. Hernandez, Gilda M. Portalatin, Tara P. Chambers, Jeremy W. Chambers

Biochem J (2018) 475 (21): 3471–3492.

https://doi.org/10.1042/BCJ20180603

Published: 15 November 2018

...) on the MOM. BH3 profiling and examination of Bcl-2 and BH3-only protein concentrations revealed that cells with high Sab concentrations were primed for apoptosis, as determined by the decrease in pro-survival Bcl-2 proteins and an increase in pro-apoptotic BH3-only proteins on mitochondria. Furthermore...

View article titled, Sab concentrations indicate chemotherapeutic susceptibility in ovarian cancer cell lines

Includes: Supplementary data

Articles

Interaction of the cryptic fragment of myelin basic protein with mitochondrial voltage-dependent anion-selective channel-1 affects cell energy metabolism

Albert G. Remacle, Swathi K. Hullugundi, Jennifer Dolkas, Mila Angert, Piotr Cieplak, David Scott, Andrei V. Chernov, Veronica I. Shubayev, Alex Y. Strongin

Biochem J (2018) 475 (14): 2355–2376.

https://doi.org/10.1042/BCJ20180137

Published: 31 July 2018

..., ipsilateral nerve. glycolysis mitochondria myelin neuroinflammation voltage-gated channels Warburg effect Specialized glial cells [Schwann cells and oligodendrocytes in the peripheral and central nervous systems (PNS/CNS), respectively] assemble the myelin sheath by enwrapping their cell...

View article titled, Interaction of the cryptic fragment of myelin basic protein with mitochondrial voltage-dependent anion-selective channel-1 affects cell energy metabolism

Includes: Supplementary data

Articles

Plant mitochondrial protein import: the ins and outs

Abi S. Ghifari, Mabel Gill-Hille, Monika W. Murcha

Biochem J (2018) 475 (13): 2191–2208.

https://doi.org/10.1042/BCJ20170521

Published: 16 July 2018

... of the Biochemical Society 2018 cellular targeting mitochondria protein import It would be tempting to speculate that an additional plant-specific outer membrane receptor exists. A recent study by Li et al. [ 41 ] characterised a novel plant-specific mitochondrial β-barrel protein named Om47...

View article titled, Plant mitochondrial protein import: the ins and outs

Articles

Cancer stem cells (CSCs): metabolic strategies for their identification and eradication

In Collection

Editors' pick of review article highlights published in the Biochemical Journal in 2018 & 2019

Ernestina M. De Francesco, Federica Sotgia, Michael P. Lisanti

Biochem J (2018) 475 (9): 1611–1634.

https://doi.org/10.1042/BCJ20170164

Published: 09 May 2018

... to identify and selectively eradicate CSCs, together with the possibility to ‘force’ CSCs within certain metabolic dependences, in order to effectively target such metabolic biochemical inflexibilities. Finally, we focus on targeting mitochondria to halt CSC dissemination and effectively eradicate cancer. ©...

View article titled, Cancer stem cells (CSCs): metabolic strategies for their identification and eradication

Articles

Genetic dissection of cyclic pyranopterin monophosphate biosynthesis in plant mitochondria

Inga Kruse, Andrew E. Maclean, Lionel Hill, Janneke Balk

Biochem J (2018) 475 (2): 495–509.

https://doi.org/10.1042/BCJ20170559

Published: 31 January 2018

...Inga Kruse; Andrew E. Maclean; Lionel Hill; Janneke Balk Mitochondria play a key role in the biosynthesis of two metal cofactors, iron–sulfur (FeS) clusters and molybdenum cofactor (Moco). The two pathways intersect at several points, but a scarcity of mutants has hindered studies to better...

View article titled, Genetic dissection of cyclic pyranopterin monophosphate biosynthesis in plant mitochondria

Includes: Supplementary data

Articles

Mitochondrial DNA density homeostasis accounts for a threshold effect in a cybrid model of a human mitochondrial disease

Juvid Aryaman, Iain G. Johnston, Nick S. Jones

Biochem J (2017) 474 (23): 4019–4034.

https://doi.org/10.1042/BCJ20170651

Published: 24 November 2017

... quantification. We propose novel experiments to verify the hypotheses made here to further develop our understanding of the threshold effect and connect with rational choices for mtDNA disease therapies. MELAS mitochondria mitochondrial dysfunction mtDNA threshold effect Mitochondria are organelles...

View article titled, Mitochondrial DNA density homeostasis accounts for a threshold effect in a cybrid model of a human mitochondrial disease

Includes: Supplementary data

Articles

Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes

Andrew A. Gibb, Pawel K. Lorkiewicz, Yu-Ting Zheng, Xiang Zhang, Aruni Bhatnagar, Steven P. Jones, Bradford G. Hill

Biochem J (2017) 474 (16): 2785–2801.

https://doi.org/10.1042/BCJ20170474

Published: 07 August 2017

... and phospholipids, respectively. Pyrimidines, UDP- N -acetylhexosamine, and the fatty acyl chains of phosphatidylinositol and triglycerides showed lower 13 C incorporation under conditions of high PFK activity; the isotopologue 13 C enrichment pattern of each metabolite indicated limitations in mitochondria...

View article titled, Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes

Articles

Protein trafficking in the mitochondrial intermembrane space: mechanisms and links to human disease

Lisa MacPherson, Kostas Tokatlidis

Biochem J (2017) 474 (15): 2533–2545.

https://doi.org/10.1042/BCJ20160627

Published: 12 July 2017

...Lisa MacPherson; Kostas Tokatlidis Mitochondria fulfill a diverse range of functions in cells including oxygen metabolism, homeostasis of inorganic ions and execution of apoptosis. Biogenesis of mitochondria relies on protein import pathways that are ensured by dedicated multiprotein translocase...

View article titled, Protein trafficking in the mitochondrial intermembrane space: mechanisms and links to human disease

Articles

The Slo(w) path to identifying the mitochondrial channels responsible for ischemic protection

Charles Owen Smith, Keith Nehrke, Paul S. Brookes

Biochem J (2017) 474 (12): 2067–2094.

https://doi.org/10.1042/BCJ20160623

Published: 09 June 2017

...Charles Owen Smith; Keith Nehrke; Paul S. Brookes Mitochondria play an important role in tissue ischemia and reperfusion (IR) injury, with energetic failure and the opening of the mitochondrial permeability transition pore being the major causes of IR-induced cell death. Thus, mitochondria...

View article titled, The Slo(w) path to identifying the mitochondrial channels responsible for ischemic protection

Articles

Duplication of Drosophila melanogaster mitochondrial EF-Tu: pre-adaptation to T-arm truncation and exclusion of bulky aminoacyl residues

Aya Sato, Takuma Suematsu, Koh-ki Aihara, Kiyoshi Kita, Tsutomu Suzuki, Kimitsuna Watanabe, Takashi Ohtsuki, Yoh-ichi Watanabe

Biochem J (2017) 474 (6): 957–969.

https://doi.org/10.1042/BCJ20160929

Published: 07 March 2017

...- and T-stems of aa-tRNAs. However, nematode mitochondrial (mt) tRNAs frequently lack all or part of the T-arm that is recognized by canonical EF-Tu. We previously reported that two distinct EF-Tu species, EF-Tu1 and EF-Tu2, respectively, recognize mt tRNAs lacking T-arms and D-arms in the mitochondria...

View article titled, Duplication of Drosophila melanogaster mitochondrial EF-Tu: pre-adaptation to T-arm truncation and exclusion of bulky aminoacyl residues

Includes: Supplementary data

Articles

Real-time imaging of intracellular hydrogen peroxide in pancreatic islets

Adam Neal, Austin Rountree, Kelly Kernan, Brian Van Yserloo, Huiliang Zhang, Benjamin J. Reed, William Osborne, Wang Wang, Ian R. Sweet

Biochem J (2016) 473 (23): 4443–4456.

https://doi.org/10.1042/BCJ20160481

Published: 25 November 2016

... and pathophysiologic states. Current methods do not provide the sensitivity, specificity and spatiotemporal resolution needed for such experiments on intact cells. We developed the use of HyPer, a genetic indicator for H 2 O 2 that can be expressed in the cytosol (cyto-HyPer) or the mitochondria (mito-HyPer) of live...

View article titled, Real-time imaging of intracellular hydrogen peroxide in pancreatic islets

Includes: Supplementary data

Articles

A large-conductance calcium-regulated K + channel in human dermal fibroblast mitochondria

Anna Kicinska, Bartlomiej Augustynek, Bogusz Kulawiak, Wieslawa Jarmuszkiewicz, Adam Szewczyk, Piotr Bednarczyk

Biochem J (2016) 473 (23): 4457–4471.

https://doi.org/10.1042/BCJ20160732

Published: 25 November 2016

... of 280 ± 2 pS in a symmetrical 150 mM KCl solution. The mitoBK Ca channel was activated by the Ca 2+ and by potassium channel opener NS1619. The channel activity was irreversibly inhibited by paxilline, a selective inhibitor of the BK Ca channels. In isolated fibroblast mitochondria NS1619 depolarized...

View article titled, A large-conductance calcium-regulated K + channel in human dermal fibroblast mitochondria

Includes: Supplementary data

Articles

Being right on Q: shaping eukaryotic evolution

Dave Speijer

Biochem J (2016) 473 (22): 4103–4127.

https://doi.org/10.1042/BCJ20160647

Published: 10 November 2016

...Dave Speijer Reactive oxygen species (ROS) formation by mitochondria is an incompletely understood eukaryotic process. I proposed a kinetic model [BioEssays (2011) 33 , 88–94] in which the ratio between electrons entering the respiratory chain via FADH 2 or NADH (the F/N ratio) is a crucial...

View article titled, Being right on Q: shaping eukaryotic evolution

Includes: Supplementary data

Articles

Getting into mitochondria

Ján A. Miernyk

Biochem J (2016) 473 (21): 3755–3758.

https://doi.org/10.1042/BCJ20160667C

Published: 27 October 2016

.... Mitochondria are a class of energy-generating semi-autonomous organelles present in the eukaryotic cytoplasm. In this context, ‘semi-autonomous’ indicates that mitochondria contain independently replicating DNA. The ‘chondriome’ includes genes encoding tRNAs and rRNAs, plus genes encoding fewer than 100...

View article titled, Getting into mitochondria

Articles

The effect of chronic alcohol consumption on mitochondrial calcium handling in hepatocytes

Guoqiang Wang, Elisabeth Mémin, Ishwarya Murali, Lawrence D. Gaspers

Biochem J (2016) 473 (21): 3903–3921.

https://doi.org/10.1042/BCJ20160255

Published: 27 October 2016

...Guoqiang Wang; Elisabeth Mémin; Ishwarya Murali; Lawrence D. Gaspers The damage to liver mitochondria is universally observed in both humans and animal models after excessive alcohol consumption. Acute alcohol treatment has been shown to stimulate calcium (Ca 2+ ) release from internal stores...

View article titled, The effect of chronic alcohol consumption on mitochondrial calcium handling in hepatocytes

Articles

Import of a major mitochondrial enzyme depends on synergy between two distinct helices of its presequence

Ester Kalef-Ezra, Dimitra Kotzamani, Ioannis Zaganas, Nitsa Katrakili, Andreas Plaitakis, Kostas Tokatlidis

Biochem J (2016) 473 (18): 2813–2829.

https://doi.org/10.1042/BCJ20160535

Published: 12 September 2016

...). To attain such high levels, GDH depends on very efficient mitochondrial targeting that, for human isoenzymes hGDH1 and hGDH2, is mediated by an unusually long cleavable presequence (N53). Here, we studied the mitochondrial transport of these proteins using isolated yeast mitochondria and human cell lines...

View article titled, Import of a major mitochondrial enzyme depends on synergy between two distinct helices of its presequence

Includes: Supplementary data

Articles

Proteomic and functional analysis of proline dehydrogenase 1 link proline catabolism to mitochondrial electron transport in Arabidopsis thaliana

Cécile Cabassa-Hourton, Peter Schertl, Marianne Bordenave-Jacquemin, Kaouthar Saadallah, Anne Guivarc'h, Sandrine Lebreton, Séverine Planchais, Jennifer Klodmann, Holger Eubel, Emilie Crilat, Delphine Lefebvre-De Vos, Thanos Ghelis, Luc Richard, Chedly Abdelly, Pierre Carol, Hans-Peter Braun, Arnould Savouré

Biochem J (2016) 473 (17): 2623–2634.

https://doi.org/10.1042/BCJ20160314

Published: 30 August 2016

...; Arnould Savouré Proline accumulates in many plant species in response to environmental stresses. Upon relief from stress, proline is rapidly oxidized in mitochondria by proline dehydrogenase (ProDH) and then by pyrroline-5-carboxylate dehydrogenase (P5CDH). Two ProDH genes have been identified...

View article titled, Proteomic and functional analysis of proline dehydrogenase 1 link proline catabolism to mitochondrial electron transport in Arabidopsis thaliana

Includes: Supplementary data

Articles

Nucleocytoplasmic human O-GlcNAc transferase is sufficient for O-GlcNAcylation of mitochondrial proteins

Riccardo Trapannone, Daniel Mariappa, Andrew T. Ferenbach, Daan M.F. van Aalten

Biochem J (2016) 473 (12): 1693–1702.

https://doi.org/10.1042/BCJ20160092

Published: 10 June 2016

...: 5′-gcctg-ttgaagtcactgagtcagcagctgactacaaggacgatgacgataagtaagcggccgcg-actctagagtgag-3′ and 5′-ctcactctagagtcgcggccgcttacttatcgtcatcgt-ccttgtagtcagctgctgactcagtgacttcaacaggc-3′. Mitochondria were purified from mouse liver and HEK 293 suspension cells as described previously [ 47 ] with minor...

View article titled, Nucleocytoplasmic human O-GlcNAc transferase is sufficient for O-GlcNAcylation of mitochondrial proteins

Articles

α-Lipoic acid promotes α-tubulin hyperacetylation and blocks the turnover of mitochondria through mitophagy

Michael W. Stoner, Dharendra Thapa, Manling Zhang, Gregory A. Gibson, Michael J. Calderon, Claudette M. St. Croix, Iain Scott

Biochem J (2016) 473 (12): 1821–1830.

https://doi.org/10.1042/BCJ20160281

Published: 10 June 2016

... inhibition of mitochondrial citrate export (a source for mitochondria-derived acetyl-CoA in the cytosol), was dependent on the α-tubulin acetyltransferase (αTAT) and was coupled to a loss in function of the cytosolic histone deacetylase, HDAC6. We further demonstrate that αLA slows the flux of substrates...

View article titled, α-Lipoic acid promotes α-tubulin hyperacetylation and blocks the turnover of mitochondria through mitophagy

Articles

Long-chain acylcarnitines determine ischaemia/reperfusion-induced damage in heart mitochondria

Edgars Liepinsh, Marina Makrecka-Kuka, Kristine Volska, Janis Kuka, Elina Makarova, Unigunde Antone, Eduards Sevostjanovs, Reinis Vilskersts, Arnis Strods, Kaspars Tars, Maija Dambrova

Biochem J (2016) 473 (9): 1191–1202.

https://doi.org/10.1042/BCJ20160164

Published: 26 April 2016

... myocardium after acute ischaemia/reperfusion. The aim of the present study was to identify harmful FA intermediates and their detrimental mechanisms of action in mitochondria and the ischaemic myocardium. In the present study, we found that the long-chain acyl-CoA and acylcarnitine content is increased...

View article titled, Long-chain acylcarnitines determine ischaemia/reperfusion-induced damage in heart mitochondria

Includes: Supplementary data

Articles

The mammalian homologue of yeast Afg1 ATPase (lactation elevated 1) mediates degradation of nuclear-encoded complex IV subunits

Jana Cesnekova, Marie Rodinova, Hana Hansikova, Josef Houstek, Jiri Zeman, Lukas Stiburek

Biochem J (2016) 473 (6): 797–804.

https://doi.org/10.1042/BJ20151029

Published: 10 March 2016

... in a discontinuous sucrose density gradient (1–1.5 M) by ultracentrifugation (85000 g ) essentially as described in [ 12 ]. Optimal cell disruption was monitored using microscopy and nuclear contamination was monitored by Western blotting. To prepare submitochondrial fractions, mitochondria at a protein...

View article titled, The mammalian homologue of yeast Afg1 ATPase (lactation elevated 1) mediates degradation of nuclear-encoded complex IV subunits

Email alerts

CONNECT

EXPLORE

This Feature Is Available To Subscribers Only