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Keywords: mitochondria
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Articles
Biochem J (2024) 481 (23): 1831–1854.
Published: 04 December 2024
...Bernardo Gindri dos Santos; Niki F. Brisnovali; Leigh Goedeke Mild uncoupling of oxidative phosphorylation is an intrinsic property of all mitochondria, allowing for adjustments in cellular energy metabolism to maintain metabolic homeostasis. Small molecule uncouplers have been extensively studied...
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Biochem J (2024) 481 (18): 1203–1219.
Published: 12 September 2024
... and the Biochemical Society under a transformative agreement with JISC. AMPK C13 formaldehyde mitochondria mitochondrial respiration AMP-activated protein kinase (AMPK) is the key component of a signalling pathway that is activated either by deprivation of cellular nutrients (especially glucose [ 1...
Includes: Supplementary data
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Biochem J (2024) 481 (10): 643–651.
Published: 10 May 2024
... Thapa ( [email protected] ) * These authors contributed equally to this work. 24 7 2023 18 4 2024 29 4 2024 © 2024 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2024 antibodies lysosomes mitochondria validation...
Includes: Supplementary data
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Biochem J (2024) 481 (4): 295–312.
Published: 19 February 2024
... synthase-2 inflammatory bowel disease inflammatory cytokines microbiome mitochondria Ketone bodies (acetoacetate, β-hydroxybutyrate) are generated via ketogenesis in mitochondria using acetyl-CoA and acetoacetyl-CoA produced by β-oxidation of fatty acids [ 1 , 2 ]. This metabolic pathway...
Includes: Supplementary data
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Biochem J (2024) 481 (3): 161–175.
Published: 29 January 2024
... subunits are present in the mature proteins and corroborate mitochondrial targeting sequence predictions ( Supplementary Figure S5 ). alphafold ATP synthase cryo-electron tomography mitochondria sub-tomogram averaging The F 1 F o ATP synthase is a molecular motor ubiquitous to all living...
Includes: Multimedia, Supplementary data
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Biochem J (2024) 481 (3): 119–139.
Published: 29 January 2024
... to humans and it regulates several aspects of mitochondrial physiology and, hence, cellular functions. In neurons, mitochondria are emerging as platforms for RNA transport and local protein translation. In this review, we discuss how RNA localization to mitochondria helps to sustain mitochondrial function...
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Biochem J (2023) 480 (21): 1767–1789.
Published: 15 November 2023
... by the restoration to baseline levels of mitochondrial stress markers with the expansion of healthy mitochondria in the skeletal muscle of RIRCD patients [ 54 ]. Mitochondrial metabolism produces metabolites, including acetyl-CoA and α-ketoglutarate, which are involved in epigenetic changes that modulate gene...
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Biochem J (2023) 480 (11): 773–789.
Published: 07 June 2023
... This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . diabetes glucose homeostasis hormone secretion insulin mitochondria pancreatic beta cell Type 2 diabetes (T2D...
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Biochem J (2021) 478 (24): 4153–4167.
Published: 16 December 2021
... agreement with JISC. aging mitochondria molecular chaperones Saccharomyces cerevisiae Dietary restriction (DR) is the most robust form of environmental manipulation known to increase longevity in a range of organisms [ 1 ]. First discovered to extend the lifespan of laboratory rats [ 2...
Includes: Supplementary data
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Biochem J (2021) 478 (21): 3809–3826.
Published: 09 November 2021
... source of ROS production. Classic studies have suggested that reductions in skeletal muscle mitochondrial content and/or function contribute to lipid-induced insulin resistance; however, in recent years the role of mitochondria in the pathophysiology of insulin resistance has been gradually re-evaluated...
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Biochem J (2021) 478 (16): 3125–3143.
Published: 26 August 2021
...Fabian den Brave; Jeannine Engelke; Thomas Becker Mitochondria import about 1000 proteins that are produced as precursors on cytosolic ribosomes. Defects in mitochondrial protein import result in the accumulation of non-imported precursor proteins and proteotoxic stress. The cell is equipped...
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Biochem J (2021) 478 (13): 2539–2553.
Published: 09 July 2021
... of intracellular lipids. insulin resistance mitochondria muscle metabolism obesity skeletal muscle Skeletal muscle plays a fundamental role in the maintenance of glucose homeostasis, and as a result, a reduction in skeletal muscle insulin responsiveness is implicated in the development of type 2...
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Biochem J (2021) 478 (10): 1977–1984.
Published: 28 May 2021
... compartments. We point out the role of mitochondria as an environmental and developmental sensor organelle that regulates ROS homeostasis and downstream events and we propose future directions of research that should help better understanding the roles of ROS in germination and seedling emergence...
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Biochem J (2021) 478 (4): 749–764.
Published: 24 February 2021
.... In mitochondria, the production of ATP by oxidative phosphorylation depends on the efficient functioning of the electron transport chain (ETC) complexes I–IV and ATP synthase (also known as complex V) located on the inner mitochondrial membrane. The passage of electrons through the ETC results in the pumping...
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Biochem J (2020) 477 (21): 4085–4132.
Published: 05 November 2020
...Daniella H. Hock; David R. L. Robinson; David A. Stroud Mitochondria produce the bulk of the energy used by almost all eukaryotic cells through oxidative phosphorylation (OXPHOS) which occurs on the four complexes of the respiratory chain and the F 1 –F 0 ATPase. Mitochondrial diseases...
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Biochem J (2020) 477 (18): 3613–3623.
Published: 28 September 2020
... is not involved in this paradoxical effect of amino acid shortage. We correlate the autophagy failure with mitochondria aggregation and we show that amino acid starvation-induced autophagy is restored in lipid droplet-deficient yeast by increasing mitochondrial biomass physiologically (respiration) or genetically...
Includes: Supplementary data
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Biochem J (2020) 477 (17): 3417–3431.
Published: 17 September 2020
... a role in elevated respiration in mitochondria, it remains unclear whether thermogenic plants possess molecular mechanisms for the mitochondrial degradation of AOX. To better understand the mechanism of AOX turnover in mitochondria, we performed a series of in organello AOX degradation assays using...
Includes: Supplementary data
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Biochem J (2020) 477 (8): 1515–1524.
Published: 29 April 2020
... ). Moreover, PolyP can stimulate F 0 F 1 -ATPase activity in the presence of ATP and, importantly, can be hydrolyzed in this enzyme instead of ATP. Furthermore, PolyP can be produced in mitochondria in the presence of substrates for respiration and phosphate by the F 0 F 1 -ATPsynthase. Thus, polyP...
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Adipose biology
Biochem J (2020) 477 (3): 709–725.
Published: 14 February 2020
... of the Biochemical Society 2020 brown adipose tissue mitochondria obesity thermogenesis The significance of brown adipose tissue (BAT) in body weight regulation is under intense investigation. The study of thermogenic mechanisms in mammals may lay the foundation to exploit these biochemical pathways...
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Biochem J (2019) 476 (15): 2235–2237.
Published: 15 August 2019
...Henver S. Brunetta; Graham P. Holloway Branched-chain keto acids (BCKA) metabolism involves several well-regulated steps within mitochondria, requires cofactors, and is modulated according to the metabolic status of the cells. This regulation has made it challenging to utilize in vitro approaches...
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Biochem J (2019) 476 (12): 1805–1815.
Published: 28 June 2019
... be tuned. mitochondria molybdenum cofactor nitric oxide nitrite reduction sulfite oxidase PCR fragments of human SO cDNA (NM_000456) were cloned into pQE80L (Qiagen, Hilden, Germany) without mitochondrial targeting sequence (Δ1-79; full-length SO) using SacI and SalI as restriction...
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Biochem J (2019) 476 (1): 25–37.
Published: 07 January 2019
...David P. Scanlon; Michael W. Salter The mitochondrial proteome is estimated to contain ∼1100 proteins, the vast majority of which are nuclear-encoded, with only 13 proteins encoded by the mitochondrial genome. The import of these nuclear-encoded proteins into mitochondria was widely believed...
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Biochem J (2018) 475 (22): 3535–3560.
Published: 20 November 2018
... signaling, mitochondria and relevant pathways for the nutritional support of spermatogenesis. 9 8 2018 28 9 2018 19 10 2018 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 leptin male fertility mitochondria obesity Sertoli...
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Biochem J (2018) 475 (21): 3471–3492.
Published: 15 November 2018
...) on the MOM. BH3 profiling and examination of Bcl-2 and BH3-only protein concentrations revealed that cells with high Sab concentrations were primed for apoptosis, as determined by the decrease in pro-survival Bcl-2 proteins and an increase in pro-apoptotic BH3-only proteins on mitochondria. Furthermore...
Includes: Supplementary data
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Biochem J (2018) 475 (13): 2191–2208.
Published: 16 July 2018
... of the Biochemical Society 2018 cellular targeting mitochondria protein import It would be tempting to speculate that an additional plant-specific outer membrane receptor exists. A recent study by Li et al. [ 41 ] characterised a novel plant-specific mitochondrial β-barrel protein named Om47...
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Biochem J (2018) 475 (9): 1611–1634.
Published: 09 May 2018
... to identify and selectively eradicate CSCs, together with the possibility to ‘force’ CSCs within certain metabolic dependences, in order to effectively target such metabolic biochemical inflexibilities. Finally, we focus on targeting mitochondria to halt CSC dissemination and effectively eradicate cancer. ©...
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Biochem J (2018) 475 (2): 495–509.
Published: 31 January 2018
...Inga Kruse; Andrew E. Maclean; Lionel Hill; Janneke Balk Mitochondria play a key role in the biosynthesis of two metal cofactors, iron–sulfur (FeS) clusters and molybdenum cofactor (Moco). The two pathways intersect at several points, but a scarcity of mutants has hindered studies to better...
Includes: Supplementary data
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Biochem J (2017) 474 (23): 4019–4034.
Published: 24 November 2017
... quantification. We propose novel experiments to verify the hypotheses made here to further develop our understanding of the threshold effect and connect with rational choices for mtDNA disease therapies. MELAS mitochondria mitochondrial dysfunction mtDNA threshold effect Mitochondria are organelles...
Includes: Supplementary data
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Biochem J (2017) 474 (16): 2785–2801.
Published: 07 August 2017
... and phospholipids, respectively. Pyrimidines, UDP- N -acetylhexosamine, and the fatty acyl chains of phosphatidylinositol and triglycerides showed lower 13 C incorporation under conditions of high PFK activity; the isotopologue 13 C enrichment pattern of each metabolite indicated limitations in mitochondria...
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Biochem J (2017) 474 (15): 2533–2545.
Published: 12 July 2017
...Lisa MacPherson; Kostas Tokatlidis Mitochondria fulfill a diverse range of functions in cells including oxygen metabolism, homeostasis of inorganic ions and execution of apoptosis. Biogenesis of mitochondria relies on protein import pathways that are ensured by dedicated multiprotein translocase...
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Biochem J (2017) 474 (12): 2067–2094.
Published: 09 June 2017
...Charles Owen Smith; Keith Nehrke; Paul S. Brookes Mitochondria play an important role in tissue ischemia and reperfusion (IR) injury, with energetic failure and the opening of the mitochondrial permeability transition pore being the major causes of IR-induced cell death. Thus, mitochondria...
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Biochem J (2016) 473 (23): 4443–4456.
Published: 25 November 2016
... and pathophysiologic states. Current methods do not provide the sensitivity, specificity and spatiotemporal resolution needed for such experiments on intact cells. We developed the use of HyPer, a genetic indicator for H 2 O 2 that can be expressed in the cytosol (cyto-HyPer) or the mitochondria (mito-HyPer) of live...
Includes: Supplementary data
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Biochem J (2016) 473 (23): 4457–4471.
Published: 25 November 2016
... of 280 ± 2 pS in a symmetrical 150 mM KCl solution. The mitoBK Ca channel was activated by the Ca 2+ and by potassium channel opener NS1619. The channel activity was irreversibly inhibited by paxilline, a selective inhibitor of the BK Ca channels. In isolated fibroblast mitochondria NS1619 depolarized...
Includes: Supplementary data
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Biochem J (2016) 473 (22): 4103–4127.
Published: 10 November 2016
...Dave Speijer Reactive oxygen species (ROS) formation by mitochondria is an incompletely understood eukaryotic process. I proposed a kinetic model [BioEssays (2011) 33 , 88–94] in which the ratio between electrons entering the respiratory chain via FADH 2 or NADH (the F/N ratio) is a crucial...
Includes: Supplementary data
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Biochem J (2016) 473 (21): 3755–3758.
Published: 27 October 2016
.... Mitochondria are a class of energy-generating semi-autonomous organelles present in the eukaryotic cytoplasm. In this context, ‘semi-autonomous’ indicates that mitochondria contain independently replicating DNA. The ‘chondriome’ includes genes encoding tRNAs and rRNAs, plus genes encoding fewer than 100...
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Biochem J (2016) 473 (21): 3903–3921.
Published: 27 October 2016
...Guoqiang Wang; Elisabeth Mémin; Ishwarya Murali; Lawrence D. Gaspers The damage to liver mitochondria is universally observed in both humans and animal models after excessive alcohol consumption. Acute alcohol treatment has been shown to stimulate calcium (Ca 2+ ) release from internal stores...
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Biochem J (2016) 473 (18): 2813–2829.
Published: 12 September 2016
...). To attain such high levels, GDH depends on very efficient mitochondrial targeting that, for human isoenzymes hGDH1 and hGDH2, is mediated by an unusually long cleavable presequence (N53). Here, we studied the mitochondrial transport of these proteins using isolated yeast mitochondria and human cell lines...
Includes: Supplementary data
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Biochem J (2016) 473 (12): 1693–1702.
Published: 10 June 2016
...: 5′-gcctg-ttgaagtcactgagtcagcagctgactacaaggacgatgacgataagtaagcggccgcg-actctagagtgag-3′ and 5′-ctcactctagagtcgcggccgcttacttatcgtcatcgt-ccttgtagtcagctgctgactcagtgacttcaacaggc-3′. Mitochondria were purified from mouse liver and HEK 293 suspension cells as described previously [ 47 ] with minor...
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Biochem J (2016) 473 (9): 1191–1202.
Published: 26 April 2016
... myocardium after acute ischaemia/reperfusion. The aim of the present study was to identify harmful FA intermediates and their detrimental mechanisms of action in mitochondria and the ischaemic myocardium. In the present study, we found that the long-chain acyl-CoA and acylcarnitine content is increased...
Includes: Supplementary data
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Biochem J (2016) 473 (6): 797–804.
Published: 10 March 2016
... in a discontinuous sucrose density gradient (1–1.5 M) by ultracentrifugation (85000  g ) essentially as described in [ 12 ]. Optimal cell disruption was monitored using microscopy and nuclear contamination was monitored by Western blotting. To prepare submitochondrial fractions, mitochondria at a protein...