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Articles

Biochemical basis and therapeutic potential of mitochondrial uncoupling in cardiometabolic syndrome

Bernardo Gindri dos Santos, Niki F. Brisnovali, Leigh Goedeke

Biochem J (2024) 481 (23): 1831–1854.

https://doi.org/10.1042/BCJ20240005

Published: 04 December 2024

...Bernardo Gindri dos Santos; Niki F. Brisnovali; Leigh Goedeke Mild uncoupling of oxidative phosphorylation is an intrinsic property of all mitochondria, allowing for adjustments in cellular energy metabolism to maintain metabolic homeostasis. Small molecule uncouplers have been extensively studied...

View articletitled, Biochemical basis and therapeutic potential of mitochondrial uncoupling in cardiometabolic syndrome

Articles

The pro-drug C13 activates AMPK by two distinct mechanisms

Jordana B. Freemantle, Dinesh Shah, Dylan M. Lynch, Alessio Ciulli, Harinder S. Hundal, D. Grahame Hardie

Biochem J (2024) 481 (18): 1203–1219.

https://doi.org/10.1042/BCJ20240425

Published: 12 September 2024

... and the Biochemical Society under a transformative agreement with JISC. AMPK C13 formaldehyde mitochondria mitochondrial respiration AMP-activated protein kinase (AMPK) is the key component of a signalling pathway that is activated either by deprivation of cellular nutrients (especially glucose [ 1...

View articletitled, The pro-drug C13 activates AMPK by two distinct mechanisms

Includes: Supplementary data

Articles

Validation of GCN5L1/BLOC1S1/BLOS1 antibodies using knockout cells and tissue

Paramesha Bugga, Michael W. Stoner, Janet R. Manning, Bellina A.S. Mushala, Nisha Bhattarai, Maryam Sharifi-Sanjani, Bradley R. Webster, Dharendra Thapa, Iain Scott

Biochem J (2024) 481 (10): 643–651.

https://doi.org/10.1042/BCJ20230302

Published: 10 May 2024

... mitochondria validation GCN5L1 (also known as BLOC1S1 and BLOS1; hereafter referred to as GCN5L1) is a highly conserved small protein of 15–17 kDa, with homologues found throughout the plant and animal kingdom [ 1 ]. First identified as a human cDNA with partial sequence homology to the lysine...

View articletitled, Validation of GCN5L1/BLOC1S1/BLOS1 antibodies using knockout cells and tissue

Includes: Supplementary data

Articles

Cardiomyocyte-specific deletion of GCN5L1 reduces lysine acetylation and attenuates diastolic dysfunction in aged mice by improving cardiac fatty acid oxidation

Jackson E. Stewart, Jenna M. Crawford, William E. Mullen, Angelica Jacques, Michael W. Stoner, Iain Scott, Dharendra Thapa

Biochem J (2024) 481 (6): 423–436.

https://doi.org/10.1042/BCJ20230421

Published: 11 March 2024

... aging cardiac function fatty acid oxidation lysine acetylation mitochondria Cardiovascular disease (CVD) is the leading cause of death globally, and aging has presented as a significant CVD risk factor [ 1 , 2 ]. By the year 2030, ∼20% of the world's population will reach the age of 65...

View articletitled, Cardiomyocyte-specific deletion of GCN5L1 reduces lysine acetylation and attenuates diastolic dysfunction in aged mice by improving cardiac fatty acid oxidation

Articles

Colonic ketogenesis, a microbiota-regulated process, contributes to blood ketones and protects against colitis in mice

Kevin Bass, Sathish Sivaprakasam, Gunadharini Dharmalingam-Nandagopal, Muthusamy Thangaraju, Vadivel Ganapathy

Biochem J (2024) 481 (4): 295–312.

https://doi.org/10.1042/BCJ20230403

Published: 19 February 2024

.... These findings demonstrate a sex-dependent obligatory role for ketogenesis in protection against colonic inflammation in mice. blood ketones colonic ketogenesis 3-hydroxy-3-methylglutaryl-CoA synthase-2 inflammatory bowel disease inflammatory cytokines microbiome mitochondria Figure 3...

View articletitled, Colonic ketogenesis, a microbiota-regulated process, contributes to blood ketones and protects against colitis in mice

Includes: Supplementary data

Articles

The consequence of ATP synthase dimer angle on mitochondrial morphology studied by cryo-electron tomography

Emma Buzzard, Mathew McLaren, Piotr Bragoszewski, Andrea Brancaccio, Holly C. Ford, Bertram Daum, Patricia Kuwabara, Ian Collinson, Vicki A.M. Gold

Biochem J (2024) 481 (3): 161–175.

https://doi.org/10.1042/BCJ20230450

Published: 29 January 2024

... cryo-electron tomography mitochondria sub-tomogram averaging The F 1 F o ATP synthase is a molecular motor ubiquitous to all living organisms, required for the essential conversion of an electrochemical gradient into the universal energy currency ATP [ 1 ]. The ATP synthase is composed...

View articletitled, The consequence of ATP synthase dimer angle on mitochondrial morphology studied by cryo-electron tomography

Includes: Multimedia, Supplementary data

Articles

The role of mitochondrial RNA association for mitochondrial homeostasis in neurons

Inmaculada Segura, Angelika Harbauer

Biochem J (2024) 481 (3): 119–139.

https://doi.org/10.1042/BCJ20230110

Published: 29 January 2024

... to humans and it regulates several aspects of mitochondrial physiology and, hence, cellular functions. In neurons, mitochondria are emerging as platforms for RNA transport and local protein translation. In this review, we discuss how RNA localization to mitochondria helps to sustain mitochondrial function...

View articletitled, The role of mitochondrial RNA association for mitochondrial homeostasis in neurons

Articles

Interactions of mitochondrial and skeletal muscle biology in mitochondrial myopathy

In Collection

Awards

Valeria Di Leo, Tiago M. Bernardino Gomes, Amy E. Vincent

Biochem J (2023) 480 (21): 1767–1789.

https://doi.org/10.1042/BCJ20220233

Published: 15 November 2023

... and perinuclear mitochondria and intermyofibrillar mitochondria Human [ 86–88 ] Continuous mitochondrial network for efficient energy distribution Wild-type mice [ 91 , 92 ] Decreased cristae density with mitochondrial dysfunction Cells [ 99 ] Donut mitochondria, concentric...

View articletitled, Interactions of mitochondrial and skeletal muscle biology in mitochondrial myopathy

Articles

Seasonal cold induces divergent structural/biochemical adaptations in different skeletal muscles of Columba livia : evidence for nonshivering thermogenesis in adult birds

Punyadhara Pani, Gourabamani Swalsingh, Sunil Pani, Unmod Senapati, Bijayashree Sahu, Benudhara Pati, Subhasmita Rout, Naresh C. Bal

Biochem J (2023) 480 (17): 1397–1409.

https://doi.org/10.1042/BCJ20230245

Published: 04 September 2023

... 24 8 2023 © 2023 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2023 cold acclimatization futile Ca 2+ cycling mitochondria non-shivering thermogenesis skeletal muscle Most of the avian species have developed mechanisms to maintain...

View articletitled, Seasonal cold induces divergent structural/biochemical adaptations in different skeletal muscles of Columba livia : evidence for nonshivering thermogenesis in adult birds

Includes: Supplementary data

Articles

Mitochondrial metabolism and dynamics in pancreatic beta cell glucose sensing

In Collection

Awards

Guy A. Rutter, Vaibhav Sidarala, Brett A. Kaufman, Scott A. Soleimanpour

Biochem J (2023) 480 (11): 773–789.

https://doi.org/10.1042/BCJ20230167

Published: 07 June 2023

... by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . diabetes glucose homeostasis hormone secretion insulin mitochondria pancreatic beta cell Type 2 diabetes (T2D) affects almost 1 in 10 adults worldwide...

View articletitled, Mitochondrial metabolism and dynamics in pancreatic beta cell glucose sensing

Articles

Site I Q in mitochondrial complex I generates S1QEL-sensitive superoxide/hydrogen peroxide in both the reverse and forward reactions

Edwin T. Gibbs, II, Chad A. Lerner, Mark A. Watson, Hoi-Shan Wong, Akos A. Gerencser, Martin D. Brand

Biochem J (2023) 480 (5): 363–384.

https://doi.org/10.1042/BCJ20220611

Published: 15 March 2023

..., the endogenous matrix NAD pool will become more reduced if flow before the challenge was forward, but more oxidised if flow was reverse. Using this assay we show in the model system of isolated rat skeletal muscle mitochondria that superoxide/hydrogen peroxide production by site I Q can be equally great whether...

View articletitled, Site I Q in mitochondrial complex I generates S1QEL-sensitive superoxide/hydrogen peroxide in both the reverse and forward reactions

Articles

Proteomic analysis of dietary restriction in yeast reveals a role for Hsp26 in replicative lifespan extension

Richard Campion, Leanne Bloxam, Kimberley Burrow, Philip J. Brownridge, Daniel R. Pentland, Patricia Thomas, Campbell W. Gourlay, Claire E. Eyers, Jeff W. Barclay, Alan Morgan

Biochem J (2021) 478 (24): 4153–4167.

https://doi.org/10.1042/BCJ20210432

Published: 16 December 2021

... was enabled by the participation of University of Liverpool in an all-inclusive Read & Publish pilot with Portland Press and the Biochemical Society under a transformative agreement with JISC. aging mitochondria molecular chaperones Saccharomyces cerevisiae Correspondence: Alan...

View articletitled, Proteomic analysis of dietary restriction in yeast reveals a role for Hsp26 in replicative lifespan extension

Includes: Supplementary data

Articles

Revisiting the contribution of mitochondrial biology to the pathophysiology of skeletal muscle insulin resistance

In Collection

Celebrating 100 years of insulin research

Sara M. Frangos, David J. Bishop, Graham P. Holloway

Biochem J (2021) 478 (21): 3809–3826.

https://doi.org/10.1042/BCJ20210145

Published: 09 November 2021

... source of ROS production. Classic studies have suggested that reductions in skeletal muscle mitochondrial content and/or function contribute to lipid-induced insulin resistance; however, in recent years the role of mitochondria in the pathophysiology of insulin resistance has been gradually re-evaluated...

View articletitled, Revisiting the contribution of mitochondrial biology to the pathophysiology of skeletal muscle insulin resistance

Articles

Quality control of protein import into mitochondria

Fabian den Brave, Jeannine Engelke, Thomas Becker

Biochem J (2021) 478 (16): 3125–3143.

https://doi.org/10.1042/BCJ20190584

Published: 26 August 2021

...Fabian den Brave; Jeannine Engelke; Thomas Becker Mitochondria import about 1000 proteins that are produced as precursors on cytosolic ribosomes. Defects in mitochondrial protein import result in the accumulation of non-imported precursor proteins and proteotoxic stress. The cell is equipped...

View articletitled, Quality control of protein import into mitochondria

Articles

Insulin rapidly increases skeletal muscle mitochondrial ADP sensitivity in the absence of a high lipid environment

Henver S. Brunetta, Heather L. Petrick, Bayley Vachon, Everson A. Nunes, Graham P. Holloway

Biochem J (2021) 478 (13): 2539–2553.

https://doi.org/10.1042/BCJ20210264

Published: 09 July 2021

... ) insulin resistance mitochondria muscle metabolism obesity skeletal muscle Skeletal muscle plays a fundamental role in the maintenance of glucose homeostasis, and as a result, a reduction in skeletal muscle insulin responsiveness is implicated in the development of type 2 diabetes mellitus...

View articletitled, Insulin rapidly increases skeletal muscle mitochondrial ADP sensitivity in the absence of a high lipid environment

Articles

Oxidative signalling in seed germination and early seedling growth: an emerging role for ROS trafficking and inter-organelle communication

Christophe Bailly, Livia Merendino

Biochem J (2021) 478 (10): 1977–1984.

https://doi.org/10.1042/BCJ20200934

Published: 28 May 2021

... compartments. We point out the role of mitochondria as an environmental and developmental sensor organelle that regulates ROS homeostasis and downstream events and we propose future directions of research that should help better understanding the roles of ROS in germination and seedling emergence. We claim...

View articletitled, Oxidative signalling in seed germination and early seedling growth: an emerging role for ROS trafficking and inter-organelle communication

Articles

The role of mitochondria in cocaine addiction

Claire Thornton, Etty Grad, Rami Yaka

Biochem J (2021) 478 (4): 749–764.

https://doi.org/10.1042/BCJ20200615

Published: 24 February 2021

... influence of cocaine, respectively. Inset: chemical structure of cocaine, a weak base. TCA, tricarboxylic acid cycle; mtDNA, mitochondrial DNA. In mitochondria, the production of ATP by oxidative phosphorylation depends on the efficient functioning of the electron transport chain (ETC) complexes I–IV...

View articletitled, The role of mitochondria in cocaine addiction

Articles

Blackout in the powerhouse: clinical phenotypes associated with defects in the assembly of OXPHOS complexes and the mitoribosome

Daniella H. Hock, David R. L. Robinson, David A. Stroud

Biochem J (2020) 477 (21): 4085–4132.

https://doi.org/10.1042/BCJ20190767

Published: 05 November 2020

...Daniella H. Hock; David R. L. Robinson; David A. Stroud Mitochondria produce the bulk of the energy used by almost all eukaryotic cells through oxidative phosphorylation (OXPHOS) which occurs on the four complexes of the respiratory chain and the F 1 –F 0 ATPase. Mitochondrial diseases...

View articletitled, Blackout in the powerhouse: clinical phenotypes associated with defects in the assembly of OXPHOS complexes and the mitoribosome

Articles

Amino acid starvation inhibits autophagy in lipid droplet-deficient cells through mitochondrial dysfunction

Pierre Voisin, Marianne Bernard, Thierry Bergès, Matthieu Régnacq

Biochem J (2020) 477 (18): 3613–3623.

https://doi.org/10.1042/BCJ20200551

Published: 28 September 2020

... is not involved in this paradoxical effect of amino acid shortage. We correlate the autophagy failure with mitochondria aggregation and we show that amino acid starvation-induced autophagy is restored in lipid droplet-deficient yeast by increasing mitochondrial biomass physiologically (respiration) or genetically...

View articletitled, Amino acid starvation inhibits autophagy in lipid droplet-deficient cells through mitochondrial dysfunction

Includes: Supplementary data

Articles

Degradation of mitochondrial alternative oxidase in the appendices of Arum maculatum

Kikukatsu Ito, Takafumi Ogata, Takanari Seito, Yui Umekawa, Yusuke Kakizaki, Hiroshi Osada, Anthony L. Moore

Biochem J (2020) 477 (17): 3417–3431.

https://doi.org/10.1042/BCJ20200515

Published: 17 September 2020

... a role in elevated respiration in mitochondria, it remains unclear whether thermogenic plants possess molecular mechanisms for the mitochondrial degradation of AOX. To better understand the mechanism of AOX turnover in mitochondria, we performed a series of in organello AOX degradation assays using...

View articletitled, Degradation of mitochondrial alternative oxidase in the appendices of Arum maculatum

Includes: Supplementary data

Articles

Inorganic polyphosphate is produced and hydrolyzed in F 0 F 1 -ATP synthase of mammalian mitochondria

Artyom Y. Baev, Plamena R. Angelova, Andrey Y. Abramov

Biochem J (2020) 477 (8): 1515–1524.

https://doi.org/10.1042/BCJ20200042

Published: 29 April 2020

... ). Moreover, PolyP can stimulate F 0 F 1 -ATPase activity in the presence of ATP and, importantly, can be hydrolyzed in this enzyme instead of ATP. Furthermore, PolyP can be produced in mitochondria in the presence of substrates for respiration and phosphate by the F 0 F 1 -ATPsynthase. Thus, polyP...

View articletitled, Inorganic polyphosphate is produced and hydrolyzed in F 0 F 1 -ATP synthase of mammalian mitochondria

Articles

UCP1-independent thermogenesis

In Collection

Adipose biology

Anna Roesler, Lawrence Kazak

Biochem J (2020) 477 (3): 709–725.

https://doi.org/10.1042/BCJ20190463

Published: 14 February 2020

... that lies in refining our understanding of the diversity of biochemical pathways controlling thermogenic respiration. brown adipose tissue mitochondria obesity thermogenesis The significance of brown adipose tissue (BAT) in body weight regulation is under intense investigation. The study...

View articletitled, UCP1-independent thermogenesis

Articles

Lack of mitochondrial NADP(H)-transhydrogenase expression in macrophages exacerbates atherosclerosis in hypercholesterolemic mice

Alessandro G. Salerno, Thiago Rentz, Gabriel G. Dorighello, Ana Carolina Marques, Estela Lorza-Gil, Amarylis C. B. A. Wanschel, Audrey de Moraes, Anibal E. Vercesi, Helena C. F. Oliveira

Biochem J (2019) 476 (24): 3769–3789.

https://doi.org/10.1042/BCJ20190543

Published: 20 December 2019

... oxidative stress and increase atherosclerosis development. Thus, we compared peritoneal macrophages and liver mitochondria from three C57BL/6J mice lines: Ldlr and Nnt double mutant, single Nnt mutant and wild-type. We found increased oxidants production in both mitochondria and macrophages according...

View articletitled, Lack of mitochondrial NADP(H)-transhydrogenase expression in macrophages exacerbates atherosclerosis in hypercholesterolemic mice

Includes: Supplementary data

Articles

Mitofusins modulate the increase in mitochondrial length, bioenergetics and secretory phenotype in therapy-induced senescent melanoma cells

In Collection

Editors' pick of research highlights published in the Biochemical Journal in 2018 & 2019

Jennyfer Martínez, Doménica Tarallo, Laura Martínez-Palma, Sabina Victoria, Mariana Bresque, Sebastián Rodríguez-Bottero, Inés Marmisolle, Carlos Escande, Patricia Cassina, Gabriela Casanova, Mariela Bollati-Fogolín, Caroline Agorio, María Moreno, Celia Quijano

Biochem J (2019) 476 (17): 2463–2486.

https://doi.org/10.1042/BCJ20190405

Published: 10 September 2019

... metabolism; and highlight mitochondria as potential pharmacological targets to modulate senescence and the SASP. In senescent cells, proliferation arrest involves the increase in cyclin-dependent kinase inhibitor 2A (p16-INK4), and/or tumor suppressor p53 that promotes the transcription of cyclin-dependent...

View articletitled, Mitofusins modulate the increase in mitochondrial length, bioenergetics and secretory phenotype in therapy-induced senescent melanoma cells

Includes: Supplementary data

Articles

New tools for an old question: dependence of ATP and bicarbonate for branched-chain keto acids oxidation

Henver S. Brunetta, Graham P. Holloway

Biochem J (2019) 476 (15): 2235–2237.

https://doi.org/10.1042/BCJ20190368

Published: 15 August 2019

...Henver S. Brunetta; Graham P. Holloway Branched-chain keto acids (BCKA) metabolism involves several well-regulated steps within mitochondria, requires cofactors, and is modulated according to the metabolic status of the cells. This regulation has made it challenging to utilize in vitro approaches...

View articletitled, New tools for an old question: dependence of ATP and bicarbonate for branched-chain keto acids oxidation

Articles

Mechanism of nitrite-dependent NO synthesis by human sulfite oxidase

Daniel Bender, Alexander Tobias Kaczmarek, Dimitri Niks, Russ Hille, Guenter Schwarz

Biochem J (2019) 476 (12): 1805–1815.

https://doi.org/10.1042/BCJ20190143

Published: 28 June 2019

...). Published by Portland Press Limited on behalf of the Biochemical Society 2019 mitochondria molybdenum cofactor nitric oxide nitrite reduction sulfite oxidase In mammals, two biochemical pathways are known for the de novo generation of nitric oxide (NO): an oxidative pathway catalyzed...

View articletitled, Mechanism of nitrite-dependent NO synthesis by human sulfite oxidase

Articles

Tissue-specific characterization of mitochondrial branched-chain keto acid oxidation using a multiplexed assay platform

Emma J. Goldberg, Katherine A. Buddo, Kelsey L. McLaughlin, Regina F. Fernandez, Andrea S. Pereyra, Christine E. Psaltis, Chien-Te Lin, James T. Hagen, Ilya N. Boykov, Tiffany K. Nguyen, Kymberly M. Gowdy, Jessica M. Ellis, P. Darrell Neufer, Joseph M. McClung, Kelsey H. Fisher-Wellman

Biochem J (2019) 476 (10): 1521–1537.

https://doi.org/10.1042/BCJ20190182

Published: 31 May 2019

... conditions, the underlying biochemical mechanism(s) within the mitochondrion remain largely unknown. In vitro experiments using isolated mitochondria represent a powerful biochemical tool for elucidating the role of the mitochondrion in driving disease. Such analyses have routinely been utilized across...

View articletitled, Tissue-specific characterization of mitochondrial branched-chain keto acid oxidation using a multiplexed assay platform

Includes: Supplementary data

Articles

Strangers in strange lands: mitochondrial proteins found at extra-mitochondrial locations

David P. Scanlon, Michael W. Salter

Biochem J (2019) 476 (1): 25–37.

https://doi.org/10.1042/BCJ20180473

Published: 07 January 2019

...David P. Scanlon; Michael W. Salter The mitochondrial proteome is estimated to contain ∼1100 proteins, the vast majority of which are nuclear-encoded, with only 13 proteins encoded by the mitochondrial genome. The import of these nuclear-encoded proteins into mitochondria was widely believed...

View articletitled, Strangers in strange lands: mitochondrial proteins found at extra-mitochondrial locations

Articles

Insights into leptin signaling and male reproductive health: the missing link between overweight and subfertility?

Bruno P. Moreira, Mariana P. Monteiro, Mário Sousa, Pedro F. Oliveira, Marco G. Alves

Biochem J (2018) 475 (22): 3535–3560.

https://doi.org/10.1042/BCJ20180631

Published: 20 November 2018

... signaling, mitochondria and relevant pathways for the nutritional support of spermatogenesis. Leptin signaling dysfunction seems to be associated with obesity-related inflammation via ER stress, through activation of specific inflammatory signaling pathways. ER is responsible for the correct folding...

View articletitled, Insights into leptin signaling and male reproductive health: the missing link between overweight and subfertility?

Articles

Sab concentrations indicate chemotherapeutic susceptibility in ovarian cancer cell lines

Iru Paudel, Sean M. Hernandez, Gilda M. Portalatin, Tara P. Chambers, Jeremy W. Chambers

Biochem J (2018) 475 (21): 3471–3492.

https://doi.org/10.1042/BCJ20180603

Published: 15 November 2018

...) on the MOM. BH3 profiling and examination of Bcl-2 and BH3-only protein concentrations revealed that cells with high Sab concentrations were primed for apoptosis, as determined by the decrease in pro-survival Bcl-2 proteins and an increase in pro-apoptotic BH3-only proteins on mitochondria. Furthermore...

View articletitled, Sab concentrations indicate chemotherapeutic susceptibility in ovarian cancer cell lines

Includes: Supplementary data

Articles

Interaction of the cryptic fragment of myelin basic protein with mitochondrial voltage-dependent anion-selective channel-1 affects cell energy metabolism

Albert G. Remacle, Swathi K. Hullugundi, Jennifer Dolkas, Mila Angert, Piotr Cieplak, David Scott, Andrei V. Chernov, Veronica I. Shubayev, Alex Y. Strongin

Biochem J (2018) 475 (14): 2355–2376.

https://doi.org/10.1042/BCJ20180137

Published: 31 July 2018

...), and polyclonal α-tubulin (#2144) antibodies were obtained from Cell Signaling Technology. A murine monoclonal antibody to LRP-1 (clone 8G1; #ab20384) was from Abcam. The secondary species-specific antibodies conjugated with either Alexa Fluor 488 or Alexa Fluor 594 were from Molecular Probes. The mitochondria...

View articletitled, Interaction of the cryptic fragment of myelin basic protein with mitochondrial voltage-dependent anion-selective channel-1 affects cell energy metabolism

Includes: Supplementary data

Articles

Plant mitochondrial protein import: the ins and outs

Abi S. Ghifari, Mabel Gill-Hille, Monika W. Murcha

Biochem J (2018) 475 (13): 2191–2208.

https://doi.org/10.1042/BCJ20170521

Published: 16 July 2018

... of the Biochemical Society 2018 cellular targeting mitochondria protein import The majority of mitochondrial precursor proteins contain either cleavable peptide extensions known as presequences or internal signals that serve as sorting signals to the mitochondria [ 19 , 20 ]. These signals...

View articletitled, Plant mitochondrial protein import: the ins and outs

Articles

Cancer stem cells (CSCs): metabolic strategies for their identification and eradication

In Collection

Editors' pick of review article highlights published in the Biochemical Journal in 2018 & 2019

Ernestina M. De Francesco, Federica Sotgia, Michael P. Lisanti

Biochem J (2018) 475 (9): 1611–1634.

https://doi.org/10.1042/BCJ20170164

Published: 09 May 2018

... to identify and selectively eradicate CSCs, together with the possibility to ‘force’ CSCs within certain metabolic dependences, in order to effectively target such metabolic biochemical inflexibilities. Finally, we focus on targeting mitochondria to halt CSC dissemination and effectively eradicate cancer...

View articletitled, Cancer stem cells (CSCs): metabolic strategies for their identification and eradication

Articles

Genetic dissection of cyclic pyranopterin monophosphate biosynthesis in plant mitochondria

Inga Kruse, Andrew E. Maclean, Lionel Hill, Janneke Balk

Biochem J (2018) 475 (2): 495–509.

https://doi.org/10.1042/BCJ20170559

Published: 31 January 2018

...Inga Kruse; Andrew E. Maclean; Lionel Hill; Janneke Balk Mitochondria play a key role in the biosynthesis of two metal cofactors, iron–sulfur (FeS) clusters and molybdenum cofactor (Moco). The two pathways intersect at several points, but a scarcity of mutants has hindered studies to better...

View articletitled, Genetic dissection of cyclic pyranopterin monophosphate biosynthesis in plant mitochondria

Includes: Supplementary data

Articles

Mitochondrial DNA density homeostasis accounts for a threshold effect in a cybrid model of a human mitochondrial disease

Juvid Aryaman, Iain G. Johnston, Nick S. Jones

Biochem J (2017) 474 (23): 4019–4034.

https://doi.org/10.1042/BCJ20170651

Published: 24 November 2017

... quantification. We propose novel experiments to verify the hypotheses made here to further develop our understanding of the threshold effect and connect with rational choices for mtDNA disease therapies. Mitochondria are organelles which are present across much of eukaryotic life and are known for their role...

View articletitled, Mitochondrial DNA density homeostasis accounts for a threshold effect in a cybrid model of a human mitochondrial disease

Includes: Supplementary data

Articles

Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes

Andrew A. Gibb, Pawel K. Lorkiewicz, Yu-Ting Zheng, Xiang Zhang, Aruni Bhatnagar, Steven P. Jones, Bradford G. Hill

Biochem J (2017) 474 (16): 2785–2801.

https://doi.org/10.1042/BCJ20170474

Published: 07 August 2017

... and phospholipids, respectively. Pyrimidines, UDP- N -acetylhexosamine, and the fatty acyl chains of phosphatidylinositol and triglycerides showed lower 13 C incorporation under conditions of high PFK activity; the isotopologue 13 C enrichment pattern of each metabolite indicated limitations in mitochondria...

View articletitled, Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes

Articles

Protein trafficking in the mitochondrial intermembrane space: mechanisms and links to human disease

Lisa MacPherson, Kostas Tokatlidis

Biochem J (2017) 474 (15): 2533–2545.

https://doi.org/10.1042/BCJ20160627

Published: 12 July 2017

...Lisa MacPherson; Kostas Tokatlidis Mitochondria fulfill a diverse range of functions in cells including oxygen metabolism, homeostasis of inorganic ions and execution of apoptosis. Biogenesis of mitochondria relies on protein import pathways that are ensured by dedicated multiprotein translocase...

View articletitled, Protein trafficking in the mitochondrial intermembrane space: mechanisms and links to human disease

Articles

The Slo(w) path to identifying the mitochondrial channels responsible for ischemic protection

Charles Owen Smith, Keith Nehrke, Paul S. Brookes

Biochem J (2017) 474 (12): 2067–2094.

https://doi.org/10.1042/BCJ20160623

Published: 09 June 2017

...Charles Owen Smith; Keith Nehrke; Paul S. Brookes Mitochondria play an important role in tissue ischemia and reperfusion (IR) injury, with energetic failure and the opening of the mitochondrial permeability transition pore being the major causes of IR-induced cell death. Thus, mitochondria...

View articletitled, The Slo(w) path to identifying the mitochondrial channels responsible for ischemic protection

Articles

Duplication of Drosophila melanogaster mitochondrial EF-Tu: pre-adaptation to T-arm truncation and exclusion of bulky aminoacyl residues

Aya Sato, Takuma Suematsu, Koh-ki Aihara, Kiyoshi Kita, Tsutomu Suzuki, Kimitsuna Watanabe, Takashi Ohtsuki, Yoh-ichi Watanabe

Biochem J (2017) 474 (6): 957–969.

https://doi.org/10.1042/BCJ20160929

Published: 07 March 2017

...- and T-stems of aa-tRNAs. However, nematode mitochondrial (mt) tRNAs frequently lack all or part of the T-arm that is recognized by canonical EF-Tu. We previously reported that two distinct EF-Tu species, EF-Tu1 and EF-Tu2, respectively, recognize mt tRNAs lacking T-arms and D-arms in the mitochondria...

View articletitled, Duplication of Drosophila melanogaster mitochondrial EF-Tu: pre-adaptation to T-arm truncation and exclusion of bulky aminoacyl residues

Includes: Supplementary data

Articles

Real-time imaging of intracellular hydrogen peroxide in pancreatic islets

Adam Neal, Austin Rountree, Kelly Kernan, Brian Van Yserloo, Huiliang Zhang, Benjamin J. Reed, William Osborne, Wang Wang, Ian R. Sweet

Biochem J (2016) 473 (23): 4443–4456.

https://doi.org/10.1042/BCJ20160481

Published: 25 November 2016

... and pathophysiologic states. Current methods do not provide the sensitivity, specificity and spatiotemporal resolution needed for such experiments on intact cells. We developed the use of HyPer, a genetic indicator for H 2 O 2 that can be expressed in the cytosol (cyto-HyPer) or the mitochondria (mito-HyPer) of live...

View articletitled, Real-time imaging of intracellular hydrogen peroxide in pancreatic islets

Includes: Supplementary data

Articles

A large-conductance calcium-regulated K + channel in human dermal fibroblast mitochondria

Anna Kicinska, Bartlomiej Augustynek, Bogusz Kulawiak, Wieslawa Jarmuszkiewicz, Adam Szewczyk, Piotr Bednarczyk

Biochem J (2016) 473 (23): 4457–4471.

https://doi.org/10.1042/BCJ20160732

Published: 25 November 2016

... of 280 ± 2 pS in a symmetrical 150 mM KCl solution. The mitoBK Ca channel was activated by the Ca 2+ and by potassium channel opener NS1619. The channel activity was irreversibly inhibited by paxilline, a selective inhibitor of the BK Ca channels. In isolated fibroblast mitochondria NS1619 depolarized...

View articletitled, A large-conductance calcium-regulated K + channel in human dermal fibroblast mitochondria

Includes: Supplementary data

Articles

Being right on Q: shaping eukaryotic evolution

Dave Speijer

Biochem J (2016) 473 (22): 4103–4127.

https://doi.org/10.1042/BCJ20160647

Published: 10 November 2016

...Dave Speijer Reactive oxygen species (ROS) formation by mitochondria is an incompletely understood eukaryotic process. I proposed a kinetic model [BioEssays (2011) 33 , 88–94] in which the ratio between electrons entering the respiratory chain via FADH 2 or NADH (the F/N ratio) is a crucial...

View articletitled, Being right on Q: shaping eukaryotic evolution

Includes: Supplementary data

Articles

Getting into mitochondria

Ján A. Miernyk

Biochem J (2016) 473 (21): 3755–3758.

https://doi.org/10.1042/BCJ20160667C

Published: 27 October 2016

.... Correspondence: Ján A. Miernyk ( [email protected] ) or ( [email protected] ) 11 7 2016 19 8 2016 22 8 2016 © 2016 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2016 biogenesis bioinformatics mitochondria secondary structure...

View articletitled, Getting into mitochondria

Articles

The effect of chronic alcohol consumption on mitochondrial calcium handling in hepatocytes

Guoqiang Wang, Elisabeth Mémin, Ishwarya Murali, Lawrence D. Gaspers

Biochem J (2016) 473 (21): 3903–3921.

https://doi.org/10.1042/BCJ20160255

Published: 27 October 2016

...Guoqiang Wang; Elisabeth Mémin; Ishwarya Murali; Lawrence D. Gaspers The damage to liver mitochondria is universally observed in both humans and animal models after excessive alcohol consumption. Acute alcohol treatment has been shown to stimulate calcium (Ca 2+ ) release from internal stores...

View articletitled, The effect of chronic alcohol consumption on mitochondrial calcium handling in hepatocytes

Articles

Import of a major mitochondrial enzyme depends on synergy between two distinct helices of its presequence

Ester Kalef-Ezra, Dimitra Kotzamani, Ioannis Zaganas, Nitsa Katrakili, Andreas Plaitakis, Kostas Tokatlidis

Biochem J (2016) 473 (18): 2813–2829.

https://doi.org/10.1042/BCJ20160535

Published: 12 September 2016

...). To attain such high levels, GDH depends on very efficient mitochondrial targeting that, for human isoenzymes hGDH1 and hGDH2, is mediated by an unusually long cleavable presequence (N53). Here, we studied the mitochondrial transport of these proteins using isolated yeast mitochondria and human cell lines...

View articletitled, Import of a major mitochondrial enzyme depends on synergy between two distinct helices of its presequence

Includes: Supplementary data

Articles

Proteomic and functional analysis of proline dehydrogenase 1 link proline catabolism to mitochondrial electron transport in Arabidopsis thaliana

Cécile Cabassa-Hourton, Peter Schertl, Marianne Bordenave-Jacquemin, Kaouthar Saadallah, Anne Guivarc'h, Sandrine Lebreton, Séverine Planchais, Jennifer Klodmann, Holger Eubel, Emilie Crilat, Delphine Lefebvre-De Vos, Thanos Ghelis, Luc Richard, Chedly Abdelly, Pierre Carol, Hans-Peter Braun, Arnould Savouré

Biochem J (2016) 473 (17): 2623–2634.

https://doi.org/10.1042/BCJ20160314

Published: 30 August 2016

...; Arnould Savouré Proline accumulates in many plant species in response to environmental stresses. Upon relief from stress, proline is rapidly oxidized in mitochondria by proline dehydrogenase (ProDH) and then by pyrroline-5-carboxylate dehydrogenase (P5CDH). Two ProDH genes have been identified...

View articletitled, Proteomic and functional analysis of proline dehydrogenase 1 link proline catabolism to mitochondrial electron transport in Arabidopsis thaliana

Includes: Supplementary data

Articles

α-Lipoic acid promotes α-tubulin hyperacetylation and blocks the turnover of mitochondria through mitophagy

Michael W. Stoner, Dharendra Thapa, Manling Zhang, Gregory A. Gibson, Michael J. Calderon, Claudette M. St. Croix, Iain Scott

Biochem J (2016) 473 (12): 1821–1830.

https://doi.org/10.1042/BCJ20160281

Published: 10 June 2016

... inhibition of mitochondrial citrate export (a source for mitochondria-derived acetyl-CoA in the cytosol), was dependent on the α-tubulin acetyltransferase (αTAT) and was coupled to a loss in function of the cytosolic histone deacetylase, HDAC6. We further demonstrate that αLA slows the flux of substrates...

View articletitled, α-Lipoic acid promotes α-tubulin hyperacetylation and blocks the turnover of mitochondria through mitophagy

Articles

Nucleocytoplasmic human O-GlcNAc transferase is sufficient for O-GlcNAcylation of mitochondrial proteins

Riccardo Trapannone, Daniel Mariappa, Andrew T. Ferenbach, Daan M.F. van Aalten

Biochem J (2016) 473 (12): 1693–1702.

https://doi.org/10.1042/BCJ20160092

Published: 10 June 2016

... splicing glycobiology mitochondria O-GlcNAc transferase (OGT) O-linked N -acetylglucosamine (O-GlcNAc) post-translational modification (PTM) O-linked N -acetylglucosamine modification (O-GlcNAcylation) is a dynamic post-translational modification (PTM), characterized by the reversible...

View articletitled, Nucleocytoplasmic human O-GlcNAc transferase is sufficient for O-GlcNAcylation of mitochondrial proteins

Articles

Long-chain acylcarnitines determine ischaemia/reperfusion-induced damage in heart mitochondria

Edgars Liepinsh, Marina Makrecka-Kuka, Kristine Volska, Janis Kuka, Elina Makarova, Unigunde Antone, Eduards Sevostjanovs, Reinis Vilskersts, Arnis Strods, Kaspars Tars, Maija Dambrova

Biochem J (2016) 473 (9): 1191–1202.

https://doi.org/10.1042/BCJ20160164

Published: 26 April 2016

... myocardium after acute ischaemia/reperfusion. The aim of the present study was to identify harmful FA intermediates and their detrimental mechanisms of action in mitochondria and the ischaemic myocardium. In the present study, we found that the long-chain acyl-CoA and acylcarnitine content is increased...

View articletitled, Long-chain acylcarnitines determine ischaemia/reperfusion-induced damage in heart mitochondria

Includes: Supplementary data

Articles

The mammalian homologue of yeast Afg1 ATPase (lactation elevated 1) mediates degradation of nuclear-encoded complex IV subunits

Jana Cesnekova, Marie Rodinova, Hana Hansikova, Josef Houstek, Jiri Zeman, Lukas Stiburek

Biochem J (2016) 473 (6): 797–804.

https://doi.org/10.1042/BJ20151029

Published: 10 March 2016

... in a discontinuous sucrose density gradient (1–1.5 M) by ultracentrifugation (85000 g ) essentially as described in [ 12 ]. Optimal cell disruption was monitored using microscopy and nuclear contamination was monitored by Western blotting. To prepare submitochondrial fractions, mitochondria at a protein...

View articletitled, The mammalian homologue of yeast Afg1 ATPase (lactation elevated 1) mediates degradation of nuclear-encoded complex IV subunits

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