... canine–human therapeutics model is being developed in B-cell lymphoma through the generation of a hybridoma cell that produces a murine monoclonal antibody specific for canine CD20. The hybridoma cell produces two light chains, light chain-3, and light chain-7. However, the contribution of either light...

...Emma J. Mead; Rosalyn J. Masterton; Marc Feary; Olga Obrezanova; Lin Zhang; Robert Young; C. Mark Smales Translation initiation is on the critical pathway for the production of monoclonal antibodies (mAbs) by mammalian cells. Formation of a closed loop structure comprised of mRNA, a number...

... LKB1 S431A/S431A and LKB1 C433S/C433S knockin mice. These animals were viable, fertile and displayed no overt phenotypes. Employing a farnesylation-specific monoclonal antibody that we generated, we established by immunoprecipitation that the vast majority, if not all, of the endogenous LKB1...

.... Hilton; Andrew D. Nash; Jian-Guo Zhang; Nicos A. Nicola Gene deletion studies in mice have revealed critical roles for IL (interleukin)-4 and -13 in asthma development, with the latter controlling lung airways resistance and mucus secretion. We have now developed human neutralizing monoclonal antibodies...

... and cytokine receptors. Recently it was shown that ADAM17 is highly expressed on the surface of many cancer cells, whereas normal cells express low levels of ADAM17, implying that ADAM17 is a potential immunotherapeutic target. We have generated a monoclonal antibody against human ADAM17, which recognized...

... patch at the LG2 domain are important for receptor binding [Sasaki, Knyazev, Cheburkin, Gohring, Tisi, Ullrich, Timpl and Hohenester (2002) J. Biol. Chem. 277 , 44164–44170]. In the present study, we developed a neutralizing human monoclonal antibody, named CNTO300, for Gas6. The antibody was generated...

... 2004 1 3 2004 1 3 2004 The Biochemical Society, London ©2004 2004 biosynthesis cellular localization endoplasmic reticulum endothelin-converting enzyme-like 1 (ECEL1) heterologous expression monoclonal antibody Abbreviations used: DMEM, Dulbecco's modified Eagle's...

... and was facilitated by a cell-surface protein(s). Within 5min at 37°C, cell-bound Ib generated Na + /K + permeable channels that were blocked by Ia. However, Ib-induced channels or oligomers were not formed at 4°C. Two monoclonal antibodies raised against Ib that recognize unique, neutralizing epitopes within...

... enzyme following cleavage mediated by an unidentified protease(s), referred to as ACE secretase, in a process known as ‘shedding'. The effects of monoclonal antibodies (mAbs) to eight different epitopes on the N-terminal domain of ACE on shedding was investigated using Chinese hamster ovary cells (CHO...

... deletion of the C-terminal three residues (268-270) still retained full binding activity. Consistently, these alterations in LOX-1 impaired the recognition by a functionally blocking monoclonal antibody for LOX-1. These data demonstrated the distinct role of the lectin domain as the functional domain...

..., demonstrating that p53 can be heterogeneously modified and providing support for the use of insect cells as a model system for identifying novel signalling pathways that target p53. One p53 isoform that was reduced in its binding to the monoclonal antibody DO-1 could be stimulated in its binding to DO-1...

... be addressed (e-mail [email protected]). 16 12 1998 16 3 1999 31 3 1999 The Biochemical Society, London © 1999 1999 cDNA expression drug interaction drug metabolism enzyme kinetics monoclonal antibody Biochem. J. (1999) 340, 845 853 (Printed in Great Britain) 845...