...D. Judy Shon; Angel Kuo; Michael J. Ferracane; Stacy A. Malaker Epithelial surfaces throughout the body are coated by mucins, a class of proteins carrying domains characterized by a high density of O-glycosylated serine and threonine residues. The resulting mucosal layers form crucial host-microbe...

...Hannah Schneider; Evelin Berger; Brendan Dolan; Beatriz Martinez-Abad; Liisa Arike; Thaher Pelaseyed; Gunnar C. Hansson Transmembrane mucin MUC17 is an integral part of the glycocalyx as it covers the brush border membrane of small intestinal enterocytes and presents an extended O -glycosylated...

...Fatima Lahdaoui; Mathieu Messager; Audrey Vincent; Flora Hec; Anne Gandon; Maxime Warlaumont; Florence Renaud; Emmanuelle Leteurtre; Guillaume Piessen; Nicolas Jonckheere; Christophe Mariette; Isabelle Van Seuningen Secreted mucins are large O-glycosylated proteins that participate...

... Airway mucin secretion and MC (mast cell) degranulation must be tightly controlled for homoeostasis of the lungs and immune system respectively. We found the exocytic protein Munc18b to be highly expressed in mouse airway epithelial cells and MCs, and localized to the apical pole of airway secretory...

...Gaspar E. Cánepa; Maria Sol Degese; Alexandre Budu; Celia R. S. Garcia; Carlos A. Buscaglia TSSA (trypomastigote small surface antigen) is a polymorphic mucin-like molecule displayed on the surface of Trypanosoma cruzi trypomastigote forms. To evaluate its functional properties, we undertook...

...Ivana Urban; Lucía Boiani Santurio; Agustina Chidichimo; Hai Yu; Xi Chen; Juan Mucci; Fernán Agüero; Carlos A. Buscaglia The surface of the protozoan Trypanosoma cruzi is covered by a dense coat of mucin-type glycoconjugates, which make a pivotal contribution to parasite protection and host immune...

..., including a sialyl-Tn antigen that is one of the typical tumour-associated carbohydrate antigens expressed on various mucins. Many epithelial tumours secrete mucins into tissues and/or the bloodstream. Mouse mammary adenocarcinoma cells, TA3-Ha, produce a mucin named epiglycanin, but a subline of them, TA3...

...Karine Rousseau; Sara Kirkham; Lindsay Johnson; Brian Fitzpatrick; Marj Howard; Emily J. Adams; Duncan F. Rogers; David Knight; Peter Clegg; David J. Thornton MUC5B is the predominant polymeric mucin in human saliva [Thornton, Khan, Mehrotra, Howard, Veerman, Packer and Sheehan (1999) Glycobiology...

...Sophie Groux-Degroote; Marie-Ange Krzewinski-Recchi; Aurélie Cazet; Audrey Vincent; Sylvain Lehoux; Jean-Jacques Lafitte; Isabelle van Seuningen; Philippe Delannoy Bronchial mucins from patients suffering from CF (cystic fibrosis) exhibit glycosylation alterations, especially increased amounts...

...Martin E. Lidell; Gunnar C. Hansson MUC5AC is the main gel-forming mucin expressed by goblet cells of the airways and stomach where it protects the underlying epithelia. We expressed the C-terminal cysteine-rich part of the human MUC5AC mucin in CHO-K1 cells (Chinese-hamster ovary K1 cells) where...

... fraction corresponded to BSSL (bile-salt-stimulated lipase) and the second to associated mucins MUC1 and MUC4. These proteins present tandem repeat O-glycosylated sequences that should act as decoy receptors for the NV, depending on the combined mother/child secretor status. 1 To whom correspodence...

...Akira Seko; Jun-ichi Sumiya; Katsuko Yamashita Sulphation of galactose at the C-3 position is one of the major post-translational modifications of colorectal mucin. Thus we partially purified a Gal 3-O-sulphotransferase from porcine colonic mucosa (pGal3ST) and studied its enzymatic characteristics...

... to pulmonary infection. Glycoproteomic analysis was performed on SMG secretions collected from explanted bronchial tissue of subjects with severe lung disease, with and without CF, and controls without lung disease. Mucins MUC5B and MUC5AC were shown to be the dominant high-molecular-mass glycoprotein...

...Valérie FAUQUETTE; Michael PERRAIS; Sylvain CERULIS; Nicolas JONCKHEERE; Marie-Paule DUCOUROUBLE; Jean-Pierre AUBERT; Pascal PIGNY; Isabelle Van SEUNINGEN The human transmembrane mucin MUC4 is aberrantly expressed in 75% of pancreatic ductal adenocarcinomas, whereas no expression is found in normal...

...Catherine ROBBE; Calliope CAPON; Bernadette CODDEVILLE; Jean-Claude MICHALSKI Purified human mucins from different parts of the intestinal tract (ileum, cecum, transverse and sigmoid colon and rectum) were isolated from two individuals with blood group ALe b (A-Lewis b ). After alkaline borohydride...

... structures of mucin oligosaccharides, and in demonstrating that there are variations in structure along the sections of the gut. In contrast to previous studies, these results described by Robbe et al. show some correlation with the activities of enzymes synthesizing mucin oligosaccharides in the human colon...

...Sara LINDÉN; Thomas BORÉN; André DUBOIS; Ingemar CARLSTEDT Mucins isolated from the stomach of Rhesus monkey are oligomeric glycoproteins with a similar mass, density, glycoform profile and tissue localization as human MUC5AC and MUC6. Antibodies raised against the human mucins recognize those from...

... premalignant lesion associated with an increase in mucin-producing goblet cells and MUC4 mucin gene overexpression. However, the molecular mechanisms underlying the regulation of MUC4 by bile acids are unknown. Since total bile is a complex mixture, we undertook to identify which bile acids are responsible...

...Nicolas JONCKHEERE; Maria van der SLUIS; Amélie VELGHE; Marie-Pierre BUISINE; Marjolein SUTMULLER; Marie-Paule DUCOUROUBLE; Pascal PIGNY; Hans A. BÜLLER; Jean-Pierre AUBERT; Alexandra W. C. EINERHAND; Isabelle VAN SEUNINGEN Changes in the expression of mucin genes in gastrointestinal cancers...

... that these were localized to the ends opposite to the parts responsible for the dimerization. The C-terminus expressed in LS 174T cells formed heterodimers with the full-length wild-type MUC2, but not with the MUC5AC mucin, normally expressed in LS 174T cells. The homodimers of the MUC2 C-termini were secreted...

... that the soluble form is created early in transit to the cell surface. These studies provide the first clear evidence that membrane mucins can be released from cells by an intracellular proteolytic mechanism that leads to secretion of the soluble form of the mucin. 1 To whom correspondence should be addressed...

[email protected] ). 21 12 2001 2 4 2002 17 5 2002 17 5 2002 The Biochemical Society, London ©2002 2002 eye glycosylation mucin O-linked oligosaccharide sialyl-Le a tear film Abbreviations used: AgPAGE, agarose/polyacrylamide composite gel electrophoresis; LC-MS...

...Shari A. PRICE-SCHIAVI; Aymee PEREZ; Roy BARCO; Kermit L. CARRAWAY Sialomucin complex (SMC/Muc4) is a heterodimeric glycoprotein complex consisting of a mucin subunit ascites sialoglycoprotein-1 (ASGP-1) and a transmembrane subunit (ASGP-2), which is aberrantly expressed on the surfaces...

...Shin-ichiro IIDA; Hideyuki TAKEUCHI; Kentaro KATO; Kazuo YAMAMOTO; Tatsuro IRIMURA Mucin 2 (MUC2) is the major intestinal mucin. O-glycans are attached to MUC2 in a potentially diverse arrangement, which is crucial for their interaction with endogeneous and exogeneous lectins. In the present report...

...Julia R. DAVIES; Naila SVITACHEVA; Louise LANNEFORS; Ragnhild KORNFÄLT; Ingemar CARLSTEDT To investigate the genetic identities of the mucins secreted in cystic fibrosis (CF) airways, sputum was collected from five individuals. Samples were separated into gel and sol phases by high-speed...