...Déborah Bréa; François Meurens; Alice V. Dubois; Julien Gaillard; Claire Chevaleyre; Marie-Lise Jourdan; Nathalie Winter; Brigitte Arbeille; Mustapha Si-Tahar; Francis Gauthier; Sylvie Attucci The serine proteases released by activated polymorphonuclear neutrophils [NSPs (neutrophil serine...

... Biochemical Society 2010 haemolytic uraemic syndrome neutrophil ribosomeinactivating protein ricin Shiga toxin Toll-like receptor (TLR) Stx (Shiga toxins) are produced by pathogenic STEC (Stx-producing Escherichia coli ) strains which are responsible for severe human illnesses...

... (transfusion-related acute lung injury) and directly affect the physiology of neutrophils [PMNs (polymorphonuclear leucocytes)]. Because the G2A receptor, expressed on PMNs, has been reported to recognize lyso-PCs, we hypothesize that lyso-PC activation of G2A causes the increases in cytosolic Ca 2+ via...

... patterns of GBS Sia O-acetylation that can be manipulated by genetic and biochemical means. In vitro and in vivo assays demonstrate that this subtle modification attenuates GBS Sia-mediated neutrophil suppression and animal virulence. 1 To whom correspondence should be addressed (email allewis...

...Valdecir F. Ximenes; Ghassan J. Maghzal; Rufus Turner; Yoji Kato; Christine C. Winterbourn; Anthony J. Kettle During inflammatory events, neutrophils and platelets interact to release a variety of mediators. Neutrophils generate superoxide and hydrogen peroxide, and also discharge the haem enzyme...

... and by neutrophils with IC 50 values of approx. 1 and 6 μM respectively. Structure–activity relationships were determined for a range of aliphatic and aromatic nitroxides, and inhibition of oxidative damage to two biologically-important protein targets (albumin and perlecan) are demonstrated. Inhibition was shown...

...Katja Lehmann; Jörg P. Müller; Bernhard Schlott; Philipp Skroblin; Dagmar Barz; Johannes Norgauer; Reinhard Wetzker Neutrophils release reactive oxygen species (ROS) as part of the innate inflammatory immune response. Phosphoinositide 3-kinase γ (PI3Kγ), which is induced by the bacterial peptide N...

... susceptibility to bacterial infection. Because neutrophils bind to collagen during their locomotion in extravascular tissue towards the infected area we investigated whether glycoxidation of collagen (AGE-collagen) alters neutrophil migration. Type I collagen extracted from rat tail tendons was used for in vitro...

... NCRDs to induce viral aggregation and to increase viral uptake by neutrophils and virus-induced respiratory burst responses by these cells. These results show that antiviral activities of SP-D can be reproduced without the N-terminal and collagen domains and that cross-linking of NCRDs is essential...

...Sandrine Pacquelet; Jennifer L. Johnson; Beverly A. Ellis; Agnieszka A. Brzezinska; William S. Lane; Daniela B. Munafo; Sergio D. Catz Exposure of neutrophils to LPS (lipopolysaccharide) triggers their oxidative response. However, the relationship between the signalling downstream of TLR4 (Toll...

... characterized in human neutrophils. We provide direct evidence that CD16b constitutively partitions with both low- and high-density DRMs. Moreover, upon CD16b engagement, a significant increase in the amount of the receptor is observed in high-density DRMs. Similarly to CD16b, CD11b also resides in low...

... towards heparan sulphate-derived saccharides. Subcellular fractionation of neutrophil organelles showed the presence of enzymatic activity mainly in the same fractions as primary granules. Furthermore, PMA treatment of the neutrophils induced release of the enzyme, indicating its matrix protein nature...

...Emmanuelle ROLLET-LABELLE; Sébastien MAROIS; Kathy BARBEAU; Stephen E. MALAWISTA; Paul H. NACCACHE We have previously shown that CD32A (or FcγRIIA), one of the main opsonin receptors, was rapidly insolubilized and degraded in intact neutrophils after its cross-linking. In view of these experimental...

... injury. The Biochemical Society, London ©2003 2003 haemoglobin myeloperoxidase myoglobin neutrophil protein aggregation protein oxidation Abbreviations used: DNP, 2,4-dinitrophenylhydrazine; ESI, electrospray ionization; HDL, high-density lipoprotein; LDL, low-density lipoprotein...

... and neutrophils eliminate invading micro-orga- nisms and other foreign particles by first ingesting them into a plasma membrane-derived intracellular vacuole, or phagosome. The ingestion, or phagocytosis, is a receptor-mediated, actin- dependent process. The resulting intracellular vacuoles, termed phagosomes...

...-binding lectin, CL-43 did not induce viral or bacterial aggregation and did not enhance IAV-induced neutrophil H 2 O 2 generation. Like SP-D, CL-43 also strongly enhanced neutrophil uptake of IAV. However, the mechanism of this enhanced internalization is different from that of SP-D in that it did...

... The Biochemical Society, London ©2002 2002 heterotrimeric G-protein neutrophil PI3K PtdIns(3,4,5) P 3 signal transduction Abbreviations used: PI3K, phosphoinositide 3OH-kinase; 6H tag, hexahistidine tag; EE tag, glutamine tag; SPR, surface plasmon resonance; PtdCho, phosphatidylcholine...

... jasplakinolide leukotriene neutrophil Abbreviations used: PMN, polymorphonuclear granulocyte; 5-LO, 5-lipoxygenase; LT, leukotriene; AA, arachidonic acid; ROS, reactive oxygen species; HSA, human serum albumin; HBSS, Hank's balanced salt solution; LTB 4 , leukotriene B 4 ; 5-HETE, 5...

... 1999 28 5 1999 The Biochemical Society, London © 1999 1999 flavocytochrome b neutrophil phox proteins superoxide Biochem. J. (1999) 341, 251 255 (Printed in Great Britain) 251 RESEARCH COMMUNICATION The mechanism of activation of NADPH oxidase in the cell-free system...

[email protected] ). 25 8 1998 26 10 1998 26 11 1998 The Biochemical Society, London © 1999 1999 MAP kinase mutagenesis neutrophil phospho-peptide mapping Biochem. J. (1999) 338, 99 105 (Printed in Great Britain) 99 The major phosphorylation site of the NADPH oxidase component...