... that bind directly to Impβ and to the other ten Kaps that perform nuclear import [ 27 , 70 , 71 ]. Since the 1980s, classical-NLSs have been identified in many cargos, mostly through independent studies of the individual cargos. More recently, systematic approaches including peptide library, proteomic...

... to disease states such as viral disease and oncogenesis. We recently developed a high-throughput screen to identify specific and general inhibitors of protein nuclear import, from which ivermectin was identified as a potential inhibitor of importin α/β-mediated transport. In the present study, we...

... in this process. DnaJB6, a chaperone protein of the DnaJ/Hsp (heat-shock protein) 40 family, stabilizes Slfn1 together with its partner Hsp70, and, more importantly, it enhances the nuclear import of Slfn1. Overexpression of DnaJB6 was found to increase Slfn1 nuclear accumulation and resulted in cell-cycle arrest...

... techniques to assess the nuclear import properties of the IN protein, and show that IN interacts with members of the Imp (Importin) family of nuclear transport proteins with high affinity and exhibits rapid nuclear accumulation within an in vitro assay, indicating that IN possesses potent nucleophilic...

.... As in yeast, the bipartite nuclear localization signal is necessary for Stau1 nuclear import in mammalian cells. It is also required for Stau1 nucleolar trafficking. However, Stau1 nuclear transit seems to be regulated by mechanisms that involve cytoplasmic retention and/or facilitated nuclear export...

... effective. Current work regarding non-viral gene delivery is now focused on understanding the mechanisms involved in cell entry, endosomal escape and nuclear import of the transgene. There is now increasing evidence to suggest that additional ligands that facilitate endosomal escape or contain a nuclear...

... directly and specifically in vitro with the nuclear import receptor family member, importin β1, and that this interaction is mediated by the PDX-1 homeodomain (amino acids 146–206). Demonstrating the functional importance of the PDX-1–importin β1 interaction, microinjection of MIN6 β-cells with anti...