... nucleosome substrates, the study of multivalent chromatin interactions, and new technologies driving the next era of histone PTM research. Despite much evidence to the contrary, the nucleosome model that emerged from this time showed histone tails extended outward from the nucleosome core ( Figure 2A...

.... However, to perform these functions, they need to occupy their sites in chromatin. In the present study, we have systematically assessed TALE binding to chromatin substrates and find that in vitro TALEs bind to their target site on nucleosomes at the more accessible entry/exit sites...

... respectively. EMSAs (electrophoretic mobility-shift assays) illustrated the ability of the Pdp1 PWWP domain to bind to the nucleosome core particle, and further mutagenesis experiments indicated the crucial role of this binding activity in histone H4K20 di- and tri-methylation in yeast cells. The present study...

...Jin Zhao; Wee Leng Siew; Weiqi Sun; Norbert Lehming The nucleosomes occupying the chromosomal start sites of transcription contain the histone H2A variant H2A.Z in place of H2A. Upon galactose induction, nucleosomes are evicted from the GAL1 locus in Saccharomyces cerevisiae cells. H2A.Z (which...

.... In this study, we examine chromatin structural changes and protein interactions at the MHRE nucleosome in response to PGC-1α expression in HK2 human kidney cells. We mapped the nucleosome positions of the ERRα gene promoter and examined the changes of histone acetylation in response to PGC-1α expression...

... topoisomerase I in the presence of camptothecin on a repeated trinucleotide sequence, (TTA) 35 , lying in chromosome XIII of Saccharomyces cerevisiae , we can conclude that nucleosomes represent a physical barrier for the enzyme activity. 1 To whom correspondence should be addressed (email...

...Wenzheng ZHANG; Yoshihide HAYASHIZAKI; Bruce C. KONE Recently, a new class of histone methyltransferases that plays an indirect role in chromatin silencing by targeting a conserved lysine residue in the nucleosome core was described, namely the Dot1 (disruptor of telomeric silencing) family [Feng...

..., histone H2A has extremely strong anti-microbial properties and could therefore help contribute to protection against bacterial invasion. anti-bacterial protein epithelium mucosal immunity nucleosome The Biochemical Society, London ©2002 2002 25 6 2002 2 8 2002 6 8 2002...