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Articles

Pyroptotic executioner pore-forming protein gasdermin D forms oligomeric assembly and exhibits amyloid-like attributes that could contribute for its pore-forming function

Shamaita Chatterjee, Tarang Gupta, Gurvinder Kaur, Kausik Chattopadhyay

Biochem J (2024) 481 (22): 1679–1705.

https://doi.org/10.1042/BCJ20240416

Published: 14 November 2024

...Shamaita Chatterjee; Tarang Gupta; Gurvinder Kaur; Kausik Chattopadhyay Gasdermin D (GSDMD) is the chief executioner of inflammatory cell death or pyroptosis. During pyroptosis, proteolytic processing of GSDMD releases its N-terminal domain (NTD), which then forms large oligomeric pores...

View articletitled, Pyroptotic executioner pore-forming protein gasdermin D forms oligomeric assembly and exhibits amyloid-like attributes that could contribute for its pore-forming function

Includes: Supplementary data

Articles

Structure, dynamics and functions of UBQLNs: at the crossroads of protein quality control machinery

Tongyin Zheng, Yiran Yang, Carlos A. Castañeda

Biochem J (2020) 477 (18): 3471–3497.

https://doi.org/10.1042/BCJ20190497

Published: 23 September 2020

... to numerous types of cellular puncta, including stress-induced biomolecular condensates, autophagosomes, aggresomes, and aggregates. In this review, we focus on deciphering how UBQLNs function on a molecular level. We examine the properties of oligomerization-driven interactions among the structured...

View articletitled, Structure, dynamics and functions of UBQLNs: at the crossroads of protein quality control machinery

Articles

Characterization of the different oligomeric states of the DAN family antagonists SOSTDC1 and SOST

Gregory R. Gipson, Chandramohan Kattamuri, Magdalena Czepnik, Thomas B. Thompson

Biochem J (2020) 477 (17): 3167–3182.

https://doi.org/10.1042/BCJ20200552

Published: 04 September 2020

... studies have revealed discrepancies between the oligomerization state of certain DAN family members, with SOST (a poor antagonist of BMP signaling) forming a monomer while Grem1, Grem2, and NBL1 (more potent BMP antagonists) form non-disulfide linked dimers. The protein SOSTDC1 (Sclerostin domain...

View articletitled, Characterization of the different oligomeric states of the DAN family antagonists SOSTDC1 and SOST

Includes: Supplementary data

Articles

Toward a systems approach to cytochrome P450 ensemble: interactions of CYP2E1 with other P450 species and their impact on CYP1A2

Nadezhda Y. Davydova, Bikash Dangi, Marc A. Maldonado, Nikita E. Vavilov, Victor G. Zgoda, Dmitri R. Davydov

Biochem J (2019) 476 (23): 3661–3685.

https://doi.org/10.1042/BCJ20190532

Published: 12 December 2019

... interactions cytochrome p450 drug metabolism oligomerization protein–protein interactions The functional properties of the drug-metabolizing system of the human liver are largely determined by the properties of the ensemble of cytochromes P450, which is responsible for metabolism of over 75% of all...

View articletitled, Toward a systems approach to cytochrome P450 ensemble: interactions of CYP2E1 with other P450 species and their impact on CYP1A2

Includes: Supplementary data

Articles

Designing active RNF4 monomers by introducing a tryptophan: avidity towards E2∼Ub conjugates dictates the activity of ubiquitin RING E3 ligases

Sayani Sarkar, Adaitya Prasad Behera, Prateeka Borar, Prerana Agarwal Banka, Ajit B. Datta

Biochem J (2019) 476 (10): 1465–1482.

https://doi.org/10.1042/BCJ20180883

Published: 28 May 2019

... to compensate for weak Ub binding. On the other hand, tryptophan was omitted from dimeric RINGs during the course of evolution to prevent unwanted modifications and allow regulation of their activity through oligomerization. Correspondence: Ajit B. Datta ( [email protected] ) * These two authors...

View articletitled, Designing active RNF4 monomers by introducing a tryptophan: avidity towards E2∼Ub conjugates dictates the activity of ubiquitin RING E3 ligases

Includes: Supplementary data

Articles

An acetylation mimicking mutation, K274Q, in tau imparts neurotoxicity by enhancing tau aggregation and inhibiting tubulin polymerization

Jitendra S. Rane, Anuradha Kumari, Dulal Panda

Biochem J (2019) 476 (10): 1401–1417.

https://doi.org/10.1042/BCJ20190042

Published: 21 May 2019

... that K274Q mutation enhanced the oligomerization of tau. The K274Q mutation also strongly decreased the critical concentration for the liquid–liquid phase separation of tau. The oligomeric forms of K274Q-tau were found to be more toxic than wild tau to neuroblastoma cells. Using circular dichroism...

View articletitled, An acetylation mimicking mutation, K274Q, in tau imparts neurotoxicity by enhancing tau aggregation and inhibiting tubulin polymerization

Includes: Supplementary data

Articles

Revisiting the role of cholesterol in regulating the pore-formation mechanism of Vibrio cholerae cytolysin, a membrane-damaging β-barrel pore-forming toxin

Reema Kathuria, Anish Kumar Mondal, Rohan Sharma, Samarjit Bhattacharyya, Kausik Chattopadhyay

Biochem J (2018) 475 (19): 3039–3055.

https://doi.org/10.1042/BCJ20180387

Published: 10 October 2018

... are available, cholesterol appears to play a less critical role in the binding step. Nevertheless, in the absence of an optimal level of membrane cholesterol in the human erythrocytes, membrane-bound fraction of the toxin remains trapped in the form of abortive oligomeric assembly, devoid of functional pore...

View articletitled, Revisiting the role of cholesterol in regulating the pore-formation mechanism of Vibrio cholerae cytolysin, a membrane-damaging β-barrel pore-forming toxin

Includes: Supplementary data

Articles

Characterization of the oligomeric states of the CK2 α 2 β 2 holoenzyme in solution

Graziano Lolli, Denise Naressi, Stefania Sarno, Roberto Battistutta

Biochem J (2017) 474 (14): 2405–2416.

https://doi.org/10.1042/BCJ20170189

Published: 06 July 2017

...), dynamic light scattering (DLS), small-angle X-ray scattering (SAXS) and mutagenesis have been employed for the characterization of the oligomeric states of CK2 in solution. SAXS measurements at 0.35 M NaCl show for the first time the shape of the α 2 β 2 active monomer in solution. At 0.25 M salt, despite...

View articletitled, Characterization of the oligomeric states of the CK2 α 2 β 2 holoenzyme in solution

Includes: Supplementary data

Articles

Quaternary structures of opsin in live cells revealed by FRET spectrometry

Ashish K. Mishra, Megan Gragg, Michael R. Stoneman, Gabriel Biener, Julie A. Oliver, Przemyslaw Miszta, Slawomir Filipek, Valerică Raicu, Paul S.-H. Park

Biochem J (2016) 473 (21): 3819–3836.

https://doi.org/10.1042/BCJ20160422

Published: 27 October 2016

... on samples prepared from the retina at low temperatures. To investigate the oligomeric status of opsin in live cells at body temperatures, we utilized a novel approach called Förster resonance energy transfer spectrometry, which previously has allowed the determination of the stoichiometry and geometry (i.e...

View articletitled, Quaternary structures of opsin in live cells revealed by FRET spectrometry

Includes: Supplementary data

Articles

Zinc-induced oligomerization of zinc α2 glycoprotein reveals multiple fatty acid-binding sites

Henna Zahid, Layeque Miah, Andy M. Lau, Lea Brochard, Debolina Hati, Tam T.T. Bui, Alex F. Drake, Jayesh Gor, Stephen J. Perkins, Lindsay C. McDermott

Biochem J (2016) 473 (1): 43–54.

https://doi.org/10.1042/BJ20150836

Published: 09 December 2015

... significance of these metal sites was investigated. Analytical ultracentrifugation (AUC) and CD showed that zinc, but not other divalent metals, causes ZAG to oligomerize in solution. Thus ZAG dimers and trimers were observed in the presence of 1 and 2 mM zinc. Molecular modelling of X-ray scattering curves...

View articletitled, Zinc-induced oligomerization of zinc α2 glycoprotein reveals multiple fatty acid-binding sites

Articles

Nuclear protein kinase CLK1 uses a non-traditional docking mechanism to select physiological substrates

Malik M. Keshwani, Kendra L. Hailey, Brandon E. Aubol, Laurent Fattet, Maria L. McGlone, Patricia A. Jennings, Joseph A. Adams

Biochem J (2015) 472 (3): 329–338.

https://doi.org/10.1042/BJ20150903

Published: 27 November 2015

... by using a novel self-association mechanism. The disordered N-terminus of CLK1 induces oligomerization, a necessary event for targeting its physiological substrates the SR protein (splicing factor containing a C-terminal RS domain) family of splicing factors. Increasing the CLK1 concentration enhances...

View articletitled, Nuclear protein kinase CLK1 uses a non-traditional docking mechanism to select physiological substrates

Articles

Arc: building a bridge from viruses to memory

Cameron Day, Jason D. Shepherd

Biochem J (2015) 469 (1): e1–e3.

https://doi.org/10.1042/BJ20150487

Published: 19 June 2015

... and that purified Arc protein is capable of self-oligomerization. Intriguingly, these domains show homology with the viral capsid protein found in the gag polypeptide of most retroviruses. These studies provide insight into how Arc may regulate multiple critical cell biological processes in neurons and reveals...

View articletitled, Arc: building a bridge from viruses to memory

Articles

Structural determinants of human proton-coupled folate transporter oligomerization: role of GXXXG motifs and identification of oligomeric interfaces at transmembrane domains 3 and 6

Mike R. Wilson, Sita Kugel, Jenny Huang, Lucas J. Wilson, Patrick A. Wloszczynski, Jun Ye, Larry H. Matherly, Zhanjun Hou

Biochem J (2015) 469 (1): 33–44.

https://doi.org/10.1042/BJ20150169

Published: 19 June 2015

... -tagged wild-type (WT) hPCFT co-associated on nickel affinity columns, suggesting that the GXXXG motifs are not directly involved in hPCFT oligomerization. This was substantiated by in situ FRET experiments with co-expressed ECFP*- and YFP-tagged hPCFT. Molecular modelling of dimeric hPCFT structures...

View articletitled, Structural determinants of human proton-coupled folate transporter oligomerization: role of GXXXG motifs and identification of oligomeric interfaces at transmembrane domains 3 and 6

Includes: Supplementary data

Articles

Arc is a flexible modular protein capable of reversible self-oligomerization

Craig Myrum, Anne Baumann, Helene J. Bustad, Marte Innselset Flydal, Vincent Mariaule, Sara Alvira, Jorge Cuéllar, Jan Haavik, Jonathan Soulé, José Maria Valpuesta, José Antonio Márquez, Aurora Martinez, Clive R. Bramham

Biochem J (2015) 468 (1): 145–158.

https://doi.org/10.1042/BJ20141446

Published: 05 May 2015

..., and stability was analysed by CD-monitored thermal denaturation and differential scanning fluorimetry (DSF). Oligomerization states under different conditions were studied by dynamic light scattering (DLS) and visualized by AFM and EM. Biophysical analyses show that hArc is a modular protein with defined...

View articletitled, Arc is a flexible modular protein capable of reversible self-oligomerization

Includes: Supplementary data

Articles

Neutral sphingomyelinase-2 is a redox sensitive enzyme: role of catalytic cysteine residues in regulation of enzymatic activity through changes in oligomeric state

P. Patrick Dotson, II, Alexander A. Karakashian, Mariana N. Nikolova-Karakashian

Biochem J (2015) 465 (3): 371–382.

https://doi.org/10.1042/BJ20140665

Published: 22 January 2015

.... Mutational analysis of nSMase-2 identified within its C-terminal catalytic domain several oxidant-sensitive cysteine residues that were shown to be involved in enzyme oligomerization. Changing Cys 617 to Ser for example is a gain-of-function mutation associated with a decreased propensity for oligomerization...

View articletitled, Neutral sphingomyelinase-2 is a redox sensitive enzyme: role of catalytic cysteine residues in regulation of enzymatic activity through changes in oligomeric state

Includes: Supplementary data

Articles

New aspects of the structure and mode of action of the human cathelicidin LL-37 revealed by the intrinsic probe p -cyanophenylalanine

Daniela Xhindoli, Francesca Morgera, Ursula Zinth, Roberto Rizzo, Sabrina Pacor, Alessandro Tossi

Biochem J (2015) 465 (3): 443–457.

https://doi.org/10.1042/BJ20141016

Published: 22 January 2015

... cytometry and AFM, we have previously observed that the oligomeric human peptide appears to form discrete channels in anionic membranes, compatible with toroidal pores, whereas the monomeric RL-37 results in the rapid formation of smaller, irregular lesions [ 3 , 15 ]. Apart from pore formation, it has been...

View articletitled, New aspects of the structure and mode of action of the human cathelicidin LL-37 revealed by the intrinsic probe p -cyanophenylalanine

Includes: Supplementary data

Articles

The upstream conserved regions (UCRs) mediate homo- and hetero-oligomerization of type 4 cyclic nucleotide phosphodiesterases (PDE4s)

Moses Xie, Brigitte Blackman, Colleen Scheitrum, Delphine Mika, Elise Blanchard, Tao Lei, Marco Conti, Wito Richter

Biochem J (2014) 459 (3): 539–550.

https://doi.org/10.1042/BJ20131681

Published: 11 April 2014

... centrifugation. Our results indicate that dimerization is a conserved property of all long PDE4 forms, whereas short forms are monomers. Dimerization is mediated by the UCR domains. Given their high sequence conservation, the UCR domains mediate not only homo-oligomerization, but also hetero-oligomerization...

View articletitled, The upstream conserved regions (UCRs) mediate homo- and hetero-oligomerization of type 4 cyclic nucleotide phosphodiesterases (PDE4s)

Includes: Supplementary data

Articles

Bacillus thuringiensis Cry1A toxins are versatile proteins with multiple modes of action: two distinct pre-pores are involved in toxicity

Isabel Gómez, Jorge Sánchez, Carlos Muñoz-Garay, Violeta Matus, Sarjeet S. Gill, Mario Soberón, Alejandra Bravo

Biochem J (2014) 459 (2): 383–396.

https://doi.org/10.1042/BJ20131408

Published: 28 March 2014

... binding of the protoxin or the protease-activated toxin to the cadherin receptor, but before membrane insertion. Both pre-pores actively induce pore formation, although with different characteristics, and contribute to the insecticidal activity. We also analysed the oligomerization of the mutant Cry1AbMod...

View articletitled, Bacillus thuringiensis Cry1A toxins are versatile proteins with multiple modes of action: two distinct pre-pores are involved in toxicity

Includes: Supplementary data

Articles

Native oligomerization determines the mode of action and biological activities of human cathelicidin LL-37

Daniela Xhindoli, Sabrina Pacor, Filomena Guida, Nikolinka Antcheva, Alessandro Tossi

Biochem J (2014) 457 (2): 263–275.

https://doi.org/10.1042/BJ20131048

Published: 20 December 2013

... a lower and more medium-sensitive antimicrobial activity than the second type, but an increased capacity to stimulate host cells. Oligomerization strongly affects the mode of interaction with biological membranes and, consequently, both cytotoxicity and receptor-mediated activities. In the present study...

View articletitled, Native oligomerization determines the mode of action and biological activities of human cathelicidin LL-37

Includes: Supplementary data

Articles

Ligand binding and complex formation of galectin-3 is modulated by pH variations

Tobias von Mach, Michael C. Carlsson, Tamara Straube, Ulf Nilsson, Hakon Leffler, Ralf Jacob

Biochem J (2014) 457 (1): 107–115.

https://doi.org/10.1042/BJ20130933

Published: 10 December 2013

... subcellular domains or sort them into distinct transport pathways. In the present paper we analysed the effect of acidification on the sugar binding and self-oligomerization of galectin-3. Using a fluorescence anisotropy assay we measured decreasing galectin-3 affinities to the blood group antigen GalNAcα1-3...

View articletitled, Ligand binding and complex formation of galectin-3 is modulated by pH variations

Includes: Supplementary data

Articles

Structural characterization of amorphous calcium carbonate-binding protein: an insight into the mechanism of amorphous calcium carbonate formation

Jingtan Su, Xiao Liang, Qiang Zhou, Guiyou Zhang, Hongzhong Wang, Liping Xie, Rongqing Zhang

Biochem J (2013) 453 (2): 179–186.

https://doi.org/10.1042/BJ20130285

Published: 28 June 2013

... correspondence should be addressed (email: [email protected] ). 22 2 2013 2 5 2013 7 5 2013 7 5 2013 © The Authors Journal compilation © 2013 Biochemical Society 2013 amorphous calcium carbonate (ACC) biomineralization homology modelling oligomerization structure...

View articletitled, Structural characterization of amorphous calcium carbonate-binding protein: an insight into the mechanism of amorphous calcium carbonate formation

Includes: Supplementary data

Articles

Pivotal role of P450–P450 interactions in CYP3A4 allostery: the case of α-naphthoflavone

Dmitri R. Davydov, Nadezhda Y. Davydova, Elena V. Sineva, Irina Kufareva, James R. Halpert

Biochem J (2013) 453 (2): 219–230.

https://doi.org/10.1042/BJ20130398

Published: 28 June 2013

...Dmitri R. Davydov; Nadezhda Y. Davydova; Elena V. Sineva; Irina Kufareva; James R. Halpert We investigated the relationship between oligomerization of CYP3A4 (cytochrome P450 3A4) and its response to ANF (α-naphthoflavone), a prototypical heterotropic activator. The addition of ANF resulted in over...

View articletitled, Pivotal role of P450–P450 interactions in CYP3A4 allostery: the case of α-naphthoflavone

Articles

Modulation of CD147-induced matrix metalloproteinase activity: role of CD147 N-glycosylation

Wan Huang, Wen-Juan Luo, Ping Zhu, Juan Tang, Xiao-Ling Yu, Hong-Yong Cui, Bin Wang, Yang Zhang, Jian-Li Jiang, Zhi-Nan Chen

Biochem J (2013) 449 (2): 437–448.

https://doi.org/10.1042/BJ20120343

Published: 14 December 2012

[email protected] ). 27 2 2012 7 9 2012 24 9 2012 24 9 2012 © The Authors Journal compilation © 2013 Biochemical Society 2013 carcinoma extracellular matrix metalloproteinase inducer (EMMPRIN) matrix metalloproteinase (MMP) N-acetylglucosaminyltransferase V oligomerization...

View articletitled, Modulation of CD147-induced matrix metalloproteinase activity: role of CD147 N-glycosylation

Articles

Homodimerization and isoform-specific heterodimerization of neuroligins

Alexandros Poulopoulos, Tolga Soykan, Liam P. Tuffy, Matthieu Hammer, Frédérique Varoqueaux, Nils Brose

Biochem J (2012) 446 (2): 321–330.

https://doi.org/10.1042/BJ20120808

Published: 14 August 2012

... at distinct synapses, with corresponding neurotransmitter and subtype specificities. In the present study, we examined the interactions between the different neuroligins by isolating endogenous oligomeric complexes using in situ cross-linking on primary neurons. Examining hippocampal, striatal, cerebellar...

View articletitled, Homodimerization and isoform-specific heterodimerization of neuroligins

Includes: Supplementary data

Articles

The oligomer plasticity of the small heat-shock protein Lo18 from Oenococcus oeni influences its role in both membrane stabilization and protein protection

Magali Maitre, Stéphanie Weidmann, Aurélie Rieu, Daphna Fenel, Guy Schoehn, Christine Ebel, Jacques Coves, Jean Guzzo

Biochem J (2012) 444 (1): 97–104.

https://doi.org/10.1042/BJ20120066

Published: 26 April 2012

... when the pH increases. The best Lo18 activities are observed at pH 7 when the particle distribution contains a major proportion of dodecamers. At basic pH, particles corresponding to a dimer prevail and are thought to be the building blocks leading to oligomerization of Lo18. At acidic pH, the dimers...

View articletitled, The oligomer plasticity of the small heat-shock protein Lo18 from Oenococcus oeni influences its role in both membrane stabilization and protein protection

Includes: Supplementary data

Articles

A common structural blueprint for plant UDP-sugar-producing pyrophosphorylases

Leszek A. Kleczkowski, Matt Geisler, Elisabeth Fitzek, Malgorzata Wilczynska

Biochem J (2011) 439 (3): 375–381.

https://doi.org/10.1042/BJ20110730

Published: 13 October 2011

...-terminal domains, which may play regulatory functions. Molecular mobility between these domains plays an important role in substrate binding and catalysis. Evolutionary relationships and the role of (de)oligomerization as a regulatory mechanism are discussed. On the basis of comparison between structures...

View articletitled, A common structural blueprint for plant UDP-sugar-producing pyrophosphorylases

Includes: Supplementary data

Articles

Different domains are critical for oligomerization compatibility of different connexins

Agustín D. Martínez, Jaime Maripillán, Rodrigo Acuña, Peter J. Minogue, Viviana M. Berthoud, Eric C. Beyer

Biochem J (2011) 436 (1): 35–43.

https://doi.org/10.1042/BJ20110008

Published: 27 April 2011

...Agustín D. Martínez; Jaime Maripillán; Rodrigo Acuña; Peter J. Minogue; Viviana M. Berthoud; Eric C. Beyer Oligomerization of connexins is a critical step in gap junction channel formation. Some members of the connexin family can oligomerize with other members and form functional heteromeric...

View articletitled, Different domains are critical for oligomerization compatibility of different connexins

Includes: Supplementary data

Articles

Recombinant Deg/HtrA proteases from Synechocystis sp. PCC 6803 differ in substrate specificity, biochemical characteristics and mechanism

Pitter F. Huesgen, Helder Miranda, XuanTam Lam, Manuela Perthold, Holger Schuhmann, Iwona Adamska, Christiane Funk

Biochem J (2011) 435 (3): 733–742.

https://doi.org/10.1042/BJ20102131

Published: 13 April 2011

... formed different homo-oligomeric complexes with and without substrate, implying mechanistic differences in comparison with each other and with the well-studied Escherichia coli orthologues DegP (degradation of periplasmic proteins P) and DegS. Deletion of the PDZ domain decreased, but did not abolish...

View articletitled, Recombinant Deg/HtrA proteases from Synechocystis sp. PCC 6803 differ in substrate specificity, biochemical characteristics and mechanism

Includes: Supplementary data

Articles

Identification of the structural motif responsible for trimeric assembly of the C-terminal regulatory domains of polycystin channels PKD2L1 and PKD2

Katrina L. Molland, Anoop Narayanan, John W. Burgner, II, Dinesh A. Yernool

Biochem J (2010) 429 (1): 171–183.

https://doi.org/10.1042/BJ20091843

Published: 14 June 2010

... oligomerization. The present study investigates the aggregation state of purified full-length PKD2L1-CRD as well as truncations of CRDs from PKD2 channels. Far- and near-UV CD spectroscopy show that the full-length PKD2L1 CRD (PKD2L1-198) and the truncated PKD2 CRD (PKD2-244) are α-helical with no β-sheet, the α...

View articletitled, Identification of the structural motif responsible for trimeric assembly of the C-terminal regulatory domains of polycystin channels PKD2L1 and PKD2

Articles

Adiponectin: no longer the lone soul in the fight against insulin resistance?

Kathryn E. Davis, Philipp E. Scherer

Biochem J (2008) 416 (2): e7–e9.

https://doi.org/10.1042/BJ20082033

Published: 12 November 2008

.... W. Krawczyk S. A. Kitidis-Mitrokostas C. Revett T. Gimeno R. Lodish H. F. Molecular, biochemical and functional characterizations of C1q/TNF family members: adipose-tissue-selective expression patterns, regulation by PPAR-γ agonist, cysteine-mediated oligomerizations...

View articletitled, Adiponectin: no longer the lone soul in the fight against insulin resistance?

Articles

Cloning and characterization of dominant negative splice variants of the human histamine H 4 receptor

Richard M. van Rijn, André van Marle, Paul L. Chazot, Ellen Langemeijer, Yongjun Qin, Fiona C. Shenton, Herman D. Lim, Obbe P. Zuiderveld, Kamonchanok Sansuk, Michel Dy, Martine J. Smit, Cornelis P. Tensen, Remko A. Bakker, Rob Leurs

Biochem J (2008) 414 (1): 121–131.

https://doi.org/10.1042/BJ20071583

Published: 29 July 2008

... negative effect on H 4 R (390) functionality, as they are able to retain H 4 R (390) intracellularly and inactivate a population of H 4 R (390) , presumably via hetero-oligomerization. alternative splicing histamine H 4 receptor G-protein-coupled receptor (GPCR) isoform oligomerization...

View articletitled, Cloning and characterization of dominant negative splice variants of the human histamine H 4 receptor

Includes: Supplementary data

Articles

PRH/Hex: an oligomeric transcription factor and multifunctional regulator of cell fate

Abdenour Soufi, Padma-Sheela Jayaraman

Biochem J (2008) 412 (3): 399–413.

https://doi.org/10.1042/BJ20080035

Published: 28 May 2008

... a member of a select group of proteins that control gene expression at the transcriptional and translational levels. Recent biophysical analysis of the PRH protein has shown that it forms homo-oligomeric complexes in vivo and in vitro and that the proline-rich region of PRH forms a novel dimerization...

View articletitled, PRH/Hex: an oligomeric transcription factor and multifunctional regulator of cell fate

Articles

Structural and functional differences among human surfactant proteins SP-A1, SP-A2 and co-expressed SP-A1/SP-A2: role of supratrimeric oligomerization

Fernando Sánchez-Barbero, Germán Rivas, Wolfram Steinhilber, Cristina Casals

Biochem J (2007) 406 (3): 479–489.

https://doi.org/10.1042/BJ20070275

Published: 29 August 2007

...-A2, like SP-A2, had a higher degree of oligomerization than SP-A1, and consequently had lower K d for binding to bacterial Re-LPS (rough lipopolysaccharide), higher self-association in the presence of calcium and greater capability to aggregate Re-LPS and phospholipids than SP-A1. On the other hand...

View articletitled, Structural and functional differences among human surfactant proteins SP-A1, SP-A2 and co-expressed SP-A1/SP-A2: role of supratrimeric oligomerization

Articles

Reggie/flotillin proteins are organized into stable tetramers in membrane microdomains

Gonzalo P. Solis, Maja Hoegg, Christina Munderloh, Yvonne Schrock, Edward Malaga-Trillo, Eric Rivera-Milla, Claudia A. O. Stuermer

Biochem J (2007) 403 (2): 313–322.

https://doi.org/10.1042/BJ20061686

Published: 26 March 2007

... are resistant to 8 M urea treatment. We also show that oligomerization requires the C-terminal but not the N-terminal halves of reggie-1 and -2. Using deletion constructs, we analysed the functional relevance of the three predicted coiled-coil stretches present in the C-terminus of reggie-1. We confirmed...

View articletitled, Reggie/flotillin proteins are organized into stable tetramers in membrane microdomains

Includes: Supplementary data

Articles

Crystal structure of human phosphoribosylpyrophosphate synthetase 1 reveals a novel allosteric site

Sheng Li, Yongcheng Lu, Baozhen Peng, Jianping Ding

Biochem J (2007) 401 (1): 39–47.

https://doi.org/10.1042/BJ20061066

Published: 11 December 2006

... allosteric site nucleotide synthesis oligomerization PRPP synthetase regulation mechanism The crystal structure of wild-type hPRS1 was determined using the MR method. The final structure model contains two hPRS1 subunits forming a homodimer, six SO 4 2− ions and 183 water molecules...

View articletitled, Crystal structure of human phosphoribosylpyrophosphate synthetase 1 reveals a novel allosteric site

Includes: Supplementary data

Articles

Dimerization of ubiquilin is dependent upon the central region of the protein: evidence that the monomer, but not the dimer, is involved in binding presenilins

Diana L. Ford, Mervyn J. Monteiro

Biochem J (2006) 399 (3): 397–404.

https://doi.org/10.1042/BJ20060441

Published: 13 October 2006

... with presenilins. 1 To whom correspondence should be addressed (email [email protected] ). 23 3 2006 28 6 2006 3 7 2006 3 7 2006 The Biochemical Society, London 2006 dimerization immunoprecipitation oligomerization presenilin ubiquilin yeast two-hydrid assay...

View articletitled, Dimerization of ubiquilin is dependent upon the central region of the protein: evidence that the monomer, but not the dimer, is involved in binding presenilins

Articles

Homologous versus heterologous interactions in the bicomponent staphylococcal γ-haemolysin pore 1

Gabriella Viero, Romina Cunaccia, Gilles Prévost, Sandra Werner, Henri Monteil, Daniel Keller, Olivier Joubert, Gianfranco Menestrina, Mauro Dalla Serra

Biochem J (2006) 394 (1): 217–225.

https://doi.org/10.1042/BJ20051210

Published: 27 January 2006

... by the oligomerization of two different proteins and a still debated number of monomers. To clarify the topology of the pore, we have mutated single residues – placed near the right and left interfaces of each monomer into cysteine. The mutants were labelled with fluorescent probes, forming a donor–acceptor pair...

View articletitled, Homologous versus heterologous interactions in the bicomponent staphylococcal γ-haemolysin pore 1

Articles

Conformational changes in dynamin on GTP binding and oligomerization reported by intrinsic and extrinsic fluorescence

Elena Solomaha, H. Clive Palfrey

Biochem J (2005) 391 (3): 601–611.

https://doi.org/10.1042/BJ20050707

Published: 25 October 2005

... to bound mant-GTP [where mant stands for 2′-(3′)- O -( N -methylanthraniloyl)] suggests that the G-domain and PH domain are in close proximity (5–6 nm). Promotion of dynamin-2 oligomerization, by reduction in ionic strength or increasing protein concentration, had little effect on intrinsic dynamin...

View articletitled, Conformational changes in dynamin on GTP binding and oligomerization reported by intrinsic and extrinsic fluorescence

Articles

H-NS is a part of a thermally controlled mechanism for bacterial gene regulation

Shusuke Ono, Martin D. Goldberg, Tjelvar Olsson, Diego Esposito, Jay C. D. Hinton, John E. Ladbury

Biochem J (2005) 391 (2): 203–213.

https://doi.org/10.1042/BJ20050453

Published: 10 October 2005

... DNA microarray histone-like nucleoid structuring protein (H-NS) oligomerization temperature regulation Pathogenic bacteria sense environmental stimuli by a range of mechanisms to ensure that virulence factors are only expressed in the correct location within the host [ 1 ]. The integrated...

View articletitled, H-NS is a part of a thermally controlled mechanism for bacterial gene regulation

Includes: Supplementary data

Articles

Oligomeric states of the voltage-dependent anion channel and cytochrome c release from mitochondria

Ran ZALK, Adrian ISRAELSON, Erez S. GARTY, Heftsi AZOULAY-ZOHAR, Varda SHOSHAN-BARMATZ

Biochem J (2005) 386 (1): 73–83.

https://doi.org/10.1042/BJ20041356

Published: 08 February 2005

... channel for the passage of cytochrome c are not clear. The present study approaches this problem by addressing the oligomeric status of VDAC and its role in the induction of the permeability transition pore and cytochrome c release. Chemical cross-linking of isolated mitochondria or purified VDAC...

View articletitled, Oligomeric states of the voltage-dependent anion channel and cytochrome c release from mitochondria

Articles

Monitoring the formation of dynamic G-protein-coupled receptor–protein complexes in living cells

Kevin D. G. PFLEGER, Karin A. EIDNE

Biochem J (2005) 385 (3): 625–637.

https://doi.org/10.1042/BJ20041361

Published: 24 January 2005

... the concept of GPCR oligomerization, as well as demonstrating GPCR interactions with GPCR kinases, β-arrestins, adenylate cyclase and a subunit of an inwardly rectifying K + channel. The present review examines recent technological advances and experimental applications of FRET and BRET, discussing...

View articletitled, Monitoring the formation of dynamic G-protein-coupled receptor–protein complexes in living cells

Articles

New insights into the structure–function relationships of Rho-associated kinase: a thermodynamic and hydrodynamic study of the dimer-to-monomer transition and its kinetic implications

John D. DORAN, Xun LIU, Paul TASLIMI, Ahmad SAADAT, Ted FOX

Biochem J (2004) 384 (2): 255–262.

https://doi.org/10.1042/BJ20040344

Published: 23 November 2004

...John D. DORAN; Xun LIU; Paul TASLIMI; Ahmad SAADAT; Ted FOX The effect of the length of ROCK (Rho-associated kinase) on its oligomerization state has been investigated by analysing full-length protein and four truncated constructs using light-scattering and analytical ultracentrifugation methods...

View articletitled, New insights into the structure–function relationships of Rho-associated kinase: a thermodynamic and hydrodynamic study of the dimer-to-monomer transition and its kinetic implications

Articles

sigma54-dependent transcription activator phage shock protein F of Escherichia coli: a fragmentation approach to identify sequences that contribute to self-association

Patricia BORDES, Siva R. WIGNESHWERARAJ, Xiaodong ZHANG, Martin BUCK

Biochem J (2004) 378 (3): 735–744.

https://doi.org/10.1042/bj20031464

Published: 15 March 2004

... of self-association into oligomeric structures and use nucleotide binding and hydrolysis to regulate their biological output. The Escherichia coli transcription activator PspF (phage shock protein F) is a member of the σ 54 -dependent transcriptional activators that belong to the AAA protein family...

View articletitled, sigma54-dependent transcription activator phage shock protein F of Escherichia coli: a fragmentation approach to identify sequences that contribute to self-association

Articles

Evidence for specific tetraspanin homodimers: inhibition of palmitoylation makes cysteine residues available for cross-linking

Oleg V. KOVALENKO, Xiuwei YANG, Tatiana V. KOLESNIKOVA, Martin E. HEMLER

Biochem J (2004) 377 (2): 407–417.

https://doi.org/10.1042/bj20031037

Published: 15 January 2004

..., multicomponent tetraspanin protein complexes. Key words: CD9, cross-linking, cysteine, oligomerization, palmi- toylation, tetraspanin. INTRODUCTION Proteins of the tetraspanin family contain four transmembrane do- mains, connected by one small (EC1) and one large extracellular loop (EC2) and short intracellular...

View articletitled, Evidence for specific tetraspanin homodimers: inhibition of palmitoylation makes cysteine residues available for cross-linking

Articles

Differential functional properties of calmodulin-dependent protein kinase IIgamma variants isolated from smooth muscle

Samudra S. GANGOPADHYAY, Amy L. BARBER, Cynthia GALLANT, Zenon GRABAREK, Janet L. SMITH, Kathleen G. MORGAN

Biochem J (2003) 372 (2): 347–357.

https://doi.org/10.1042/bj20030015

Published: 01 June 2003

... 2003 19 2 2003 25 2 2003 25 2 2003 The Biochemical Society, London ©2003 2003 alternative polyadenylation alternative splicing autodephosphorylation calmodulin kinase IIγ oligomerization smooth muscle Abbreviations used: CaM, calmodulin; CaMKII, CaM-dependent...

View articletitled, Differential functional properties of calmodulin-dependent protein kinase IIgamma variants isolated from smooth muscle

Articles

The N-terminus of the human copper transporter 1 (hCTR1) is localized extracellularly, and interacts with itself

Adriana E.M. KLOMP, Jenneke A. JUIJN, Linda T.M. van der GUN, Inge E.T. van den BERG, Ruud BERGER, Leo W.J. KLOMP

Biochem J (2003) 370 (3): 881–889.

https://doi.org/10.1042/bj20021128

Published: 15 March 2003

... six times, permitting forma- tion of a channel, which is consistent with its proposed role as a copper transporter. Key words: copper transport, membrane topology, N-linked glycosylation, oligomerization, solute carrier. 35 kDa, which is expressed ubiquitously [12]. Between different cell types...

View articletitled, The N-terminus of the human copper transporter 1 (hCTR1) is localized extracellularly, and interacts with itself

Articles

COUP-TF (chicken ovalbumin upstream promoter transcription factor)-interacting protein 1 (CTIP1) is a sequence-specific DNA binding protein

Dorina AVRAM, Andrew FIELDS, Thanaset SENAWONG, Acharawan TOPARK-NGARM, Mark LEID

Biochem J (2002) 368 (2): 555–563.

https://doi.org/10.1042/bj20020496

Published: 01 December 2002

... to the canonical GC box and was bound by a CTIP1 oligomeric complex(es) in vitro . Furthermore, both CTIP1 and CTIP2 repressed transcription of a reporter gene harbouring a multimerized CTIP binding site, and this repression was neither reversed by trichostatin A (an inhibitor of known class I and II histone...

View articletitled, COUP-TF (chicken ovalbumin upstream promoter transcription factor)-interacting protein 1 (CTIP1) is a sequence-specific DNA binding protein

Articles

Oligomeric structure of proclavaminic acid amidino hydrolase: evolution of a hydrolytic enzyme in clavulanic acid biosynthesis

Jonathan M. ELKINS, Ian. J. CLIFTON, Helena HERNÁNDEZ, Linh X. DOAN, Carol V. ROBINSON, Christopher J. SCHOFIELD, Kirsty S. HEWITSON

Biochem J (2002) 366 (2): 423–434.

https://doi.org/10.1042/bj20020125

Published: 01 September 2002

...-mail [email protected] ). 21 1 2002 9 5 2002 21 5 2002 21 5 2002 The Biochemical Society, London ©2002 2002 arginase manganese enzyme oligomerization Abbreviations used: CAS, clavaminic acid synthase; CNS, crystallography and NMR system; ESI, electrospray...

View articletitled, Oligomeric structure of proclavaminic acid amidino hydrolase: evolution of a hydrolytic enzyme in clavulanic acid biosynthesis

Articles

Bid induces cytochrome c-impermeable Bax channels in liposomes

Xavier ROUCOU, Tatiana ROSTOVTSEVA, Sylvie MONTESSUIT, Jean-Claude MARTINOU, Bruno ANTONSSON

Biochem J (2002) 363 (3): 547–552.

https://doi.org/10.1042/bj3630547

Published: 24 April 2002

... translocated to mitochondria, inserts into the outer membrane, oligomerizes and triggers the release of cytochrome c , possibly by channel formation. The BH3 domain-only protein Bid induces a conformational change in Bax before its insertion into the outer membrane. The mechanism by which Bid promotes Bax...

View articletitled, Bid induces cytochrome c-impermeable Bax channels in liposomes

Articles

Transcriptional activities of p73 splicing variants are regulated by inter-variant association

Yoshihide UEDA, Makoto HIJIKATA, Shinji TAKAGI, Tsutomu CHIBA, Kunitada SHIMOTOHNO

Biochem J (2001) 356 (3): 859–866.

https://doi.org/10.1042/bj3560859

Published: 08 June 2001

.... 1 To whom correspondence should be addressed (e-mail [email protected] ). 29 1 2001 29 3 2001 17 4 2001 The Biochemical Society, London ©2001 2001 oligomerization p53 splicing variant transcription Abbreviations used: p53BCS, p53-binding consensus...

View articletitled, Transcriptional activities of p73 splicing variants are regulated by inter-variant association

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