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Keywords: oligomerization
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Articles
Biochem J (2020) 477 (18): 3471–3497.
Published: 23 September 2020
... numerous types of cellular puncta, including stress-induced biomolecular condensates, autophagosomes, aggresomes, and aggregates. In this review, we focus on deciphering how UBQLNs function on a molecular level. We examine the properties of oligomerization-driven interactions among the structured and...
Articles
Biochem J (2020) 477 (17): 3167–3182.
Published: 04 September 2020
... studies have revealed discrepancies between the oligomerization state of certain DAN family members, with SOST (a poor antagonist of BMP signaling) forming a monomer while Grem1, Grem2, and NBL1 (more potent BMP antagonists) form non-disulfide linked dimers. The protein SOSTDC1 (Sclerostin domain...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (23): 3661–3685.
Published: 12 December 2019
... ]. This effect is reminiscent of an impact of CYP2E1 on H 2 O 2 production by CYP2A6 observed by Tan et al. [ 15 ]. alcohol–drug interactions cytochrome p450 drug metabolism oligomerization protein–protein interactions The functional properties of the drug-metabolizing system of the...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (10): 1465–1482.
Published: 28 May 2019
... compensate for weak Ub binding. On the other hand, tryptophan was omitted from dimeric RINGs during the course of evolution to prevent unwanted modifications and allow regulation of their activity through oligomerization. Correspondence: Ajit B. Datta ( ajit@jcbose.ac.in ) * These two authors...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (10): 1401–1417.
Published: 21 May 2019
... that K274Q mutation enhanced the oligomerization of tau. The K274Q mutation also strongly decreased the critical concentration for the liquid–liquid phase separation of tau. The oligomeric forms of K274Q-tau were found to be more toxic than wild tau to neuroblastoma cells. Using circular dichroism and...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (19): 3039–3055.
Published: 10 October 2018
... available, cholesterol appears to play a less critical role in the binding step. Nevertheless, in the absence of an optimal level of membrane cholesterol in the human erythrocytes, membrane-bound fraction of the toxin remains trapped in the form of abortive oligomeric assembly, devoid of functional pore...
Includes: Supplementary data
Articles
Biochem J (2017) 474 (14): 2405–2416.
Published: 06 July 2017
...), dynamic light scattering (DLS), small-angle X-ray scattering (SAXS) and mutagenesis have been employed for the characterization of the oligomeric states of CK2 in solution. SAXS measurements at 0.35 M NaCl show for the first time the shape of the α 2 β 2 active monomer in solution. At 0.25 M salt, despite...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (21): 3819–3836.
Published: 27 October 2016
... on samples prepared from the retina at low temperatures. To investigate the oligomeric status of opsin in live cells at body temperatures, we utilized a novel approach called Förster resonance energy transfer spectrometry, which previously has allowed the determination of the stoichiometry and...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (1): 43–54.
Published: 09 December 2015
... significance of these metal sites was investigated. Analytical ultracentrifugation (AUC) and CD showed that zinc, but not other divalent metals, causes ZAG to oligomerize in solution. Thus ZAG dimers and trimers were observed in the presence of 1 and 2 mM zinc. Molecular modelling of X-ray scattering curves...
Articles
Biochem J (2015) 472 (3): 329–338.
Published: 27 November 2015
... by using a novel self-association mechanism. The disordered N-terminus of CLK1 induces oligomerization, a necessary event for targeting its physiological substrates the SR protein (splicing factor containing a C-terminal RS domain) family of splicing factors. Increasing the CLK1 concentration...
Articles
Biochem J (2015) 469 (1): 33–44.
Published: 19 June 2015
... His 10 -tagged wild-type (WT) hPCFT co-associated on nickel affinity columns, suggesting that the GXXXG motifs are not directly involved in hPCFT oligomerization. This was substantiated by in situ FRET experiments with co-expressed ECFP*- and YFP-tagged hPCFT. Molecular modelling of dimeric hPCFT...
Includes: Supplementary data
Articles
Biochem J (2015) 469 (1): e1–e3.
Published: 19 June 2015
... that purified Arc protein is capable of self-oligomerization. Intriguingly, these domains show homology with the viral capsid protein found in the gag polypeptide of most retroviruses. These studies provide insight into how Arc may regulate multiple critical cell biological processes in neurons and...
Articles
Biochem J (2015) 468 (1): 145–158.
Published: 05 May 2015
... stability was analysed by CD-monitored thermal denaturation and differential scanning fluorimetry (DSF). Oligomerization states under different conditions were studied by dynamic light scattering (DLS) and visualized by AFM and EM. Biophysical analyses show that hArc is a modular protein with defined...
Includes: Supplementary data
Articles
Biochem J (2015) 465 (3): 371–382.
Published: 22 January 2015
.... Mutational analysis of nSMase-2 identified within its C-terminal catalytic domain several oxidant-sensitive cysteine residues that were shown to be involved in enzyme oligomerization. Changing Cys 617 to Ser for example is a gain-of-function mutation associated with a decreased propensity for oligomerization...
Includes: Supplementary data
Articles
Biochem J (2015) 465 (3): 443–457.
Published: 22 January 2015
...-assemble in solution or in the presence of biological membranes, as a key feature of its multiple biological functions. p -cyano- L -phenylalanine LL-37 oligomerization RL-37 surface plasmon resonance time-resolved fluorescence LL-37, the only human cathelicidin, is an essential...
Includes: Supplementary data
Articles
Biochem J (2014) 459 (3): 539–550.
Published: 11 April 2014
... centrifugation. Our results indicate that dimerization is a conserved property of all long PDE4 forms, whereas short forms are monomers. Dimerization is mediated by the UCR domains. Given their high sequence conservation, the UCR domains mediate not only homo-oligomerization, but also hetero-oligomerization of...
Includes: Supplementary data
Articles
Biochem J (2014) 459 (2): 383–396.
Published: 28 March 2014
... binding of the protoxin or the protease-activated toxin to the cadherin receptor, but before membrane insertion. Both pre-pores actively induce pore formation, although with different characteristics, and contribute to the insecticidal activity. We also analysed the oligomerization of the mutant Cry1AbMod...
Includes: Supplementary data
Articles
Biochem J (2014) 457 (2): 263–275.
Published: 20 December 2013
... lower and more medium-sensitive antimicrobial activity than the second type, but an increased capacity to stimulate host cells. Oligomerization strongly affects the mode of interaction with biological membranes and, consequently, both cytotoxicity and receptor-mediated activities. In the present study...
Includes: Supplementary data
Articles
Biochem J (2014) 457 (1): 107–115.
Published: 10 December 2013
... subcellular domains or sort them into distinct transport pathways. In the present paper we analysed the effect of acidification on the sugar binding and self-oligomerization of galectin-3. Using a fluorescence anisotropy assay we measured decreasing galectin-3 affinities to the blood group antigen GalNAcα1-3...
Includes: Supplementary data
Articles
Biochem J (2013) 453 (2): 179–186.
Published: 28 June 2013
... Authors Journal compilation © 2013 Biochemical Society 2013 amorphous calcium carbonate (ACC) biomineralization homology modelling oligomerization structure–function relationship symmetry Many organisms make use of calcium carbonate as a constituent of their skeleton or shells, such as...
Includes: Supplementary data
Articles
Biochem J (2013) 453 (2): 219–230.
Published: 28 June 2013
...Dmitri R. Davydov; Nadezhda Y. Davydova; Elena V. Sineva; Irina Kufareva; James R. Halpert We investigated the relationship between oligomerization of CYP3A4 (cytochrome P450 3A4) and its response to ANF (α-naphthoflavone), a prototypical heterotropic activator. The addition of ANF resulted in over...
Articles
Biochem J (2013) 449 (2): 437–448.
Published: 14 December 2012
... metalloproteinase (MMP) N-acetylglucosaminyltransferase V oligomerization translocation Glycans are involved in each and every aspect of tumour progression, from cellular proliferation to angiogenesis and metastasis [ 1 ]. Changes in post-translational modification of proteins are closely associated with...
Articles
Biochem J (2012) 446 (2): 321–330.
Published: 14 August 2012
... distinct synapses, with corresponding neurotransmitter and subtype specificities. In the present study, we examined the interactions between the different neuroligins by isolating endogenous oligomeric complexes using in situ cross-linking on primary neurons. Examining hippocampal, striatal, cerebellar and...
Includes: Supplementary data
Articles
Biochem J (2012) 444 (1): 97–104.
Published: 26 April 2012
... when the pH increases. The best Lo18 activities are observed at pH 7 when the particle distribution contains a major proportion of dodecamers. At basic pH, particles corresponding to a dimer prevail and are thought to be the building blocks leading to oligomerization of Lo18. At acidic pH, the dimers...
Includes: Supplementary data
Articles
Biochem J (2011) 439 (3): 375–381.
Published: 13 October 2011
...-terminal domains, which may play regulatory functions. Molecular mobility between these domains plays an important role in substrate binding and catalysis. Evolutionary relationships and the role of (de)oligomerization as a regulatory mechanism are discussed. Despite sharing the same catalytic function...
Includes: Supplementary data
Articles
Biochem J (2011) 436 (1): 35–43.
Published: 27 April 2011
...Agustín D. Martínez; Jaime Maripillán; Rodrigo Acuña; Peter J. Minogue; Viviana M. Berthoud; Eric C. Beyer Oligomerization of connexins is a critical step in gap junction channel formation. Some members of the connexin family can oligomerize with other members and form functional heteromeric...
Includes: Supplementary data
Articles
Biochem J (2011) 435 (3): 733–742.
Published: 13 April 2011
... formed different homo-oligomeric complexes with and without substrate, implying mechanistic differences in comparison with each other and with the well-studied Escherichia coli orthologues DegP (degradation of periplasmic proteins P) and DegS. Deletion of the PDZ domain decreased, but did not abolish...
Includes: Supplementary data
Articles
Biochem J (2010) 429 (1): 171–183.
Published: 14 June 2010
... oligomerization. The present study investigates the aggregation state of purified full-length PKD2L1-CRD as well as truncations of CRDs from PKD2 channels. Far- and near-UV CD spectroscopy show that the full-length PKD2L1 CRD (PKD2L1-198) and the truncated PKD2 CRD (PKD2-244) are α-helical with no β-sheet, the α...
Articles
Biochem J (2008) 416 (2): e7–e9.
Published: 12 November 2008
...-related protein 30 (Acrp30) adipokine adipose tissue C1q/TNF (tumour necrosis factor α)-related protein (CTRP) insulin sensitizer oligomerization The study of adipose tissue as an endocrine organ is a highly active area of research in light of the increasing obesity pandemic. In the 15 years...
Articles
Biochem J (2008) 414 (1): 121–131.
Published: 29 July 2008
... negative effect on H 4 R (390) functionality, as they are able to retain H 4 R (390) intracellularly and inactivate a population of H 4 R (390) , presumably via hetero-oligomerization. alternative splicing histamine H 4 receptor G-protein-coupled receptor (GPCR) isoform oligomerization The...
Includes: Supplementary data
Articles
Biochem J (2008) 412 (3): 399–413.
Published: 28 May 2008
... member of a select group of proteins that control gene expression at the transcriptional and translational levels. Recent biophysical analysis of the PRH protein has shown that it forms homo-oligomeric complexes in vivo and in vitro and that the proline-rich region of PRH forms a novel dimerization...
Articles
Biochem J (2007) 406 (3): 479–489.
Published: 29 August 2007
...-A2, like SP-A2, had a higher degree of oligomerization than SP-A1, and consequently had lower K d for binding to bacterial Re-LPS (rough lipopolysaccharide), higher self-association in the presence of calcium and greater capability to aggregate Re-LPS and phospholipids than SP-A1. On the other hand...
Articles
Biochem J (2007) 403 (2): 313–322.
Published: 26 March 2007
... are resistant to 8 M urea treatment. We also show that oligomerization requires the C-terminal but not the N-terminal halves of reggie-1 and -2. Using deletion constructs, we analysed the functional relevance of the three predicted coiled-coil stretches present in the C-terminus of reggie-1. We...
Includes: Supplementary data
Articles
Biochem J (2007) 401 (1): 39–47.
Published: 11 December 2006
... Biochemical Society, London 2007 allosteric site nucleotide synthesis oligomerization PRPP synthetase regulation mechanism PRPP (phosphoribosylpyrophosphate) synthetases (ATP: D -ribose-5-phosphate pyrophosphotransferase; EC 2.7.6.1) are a family of enzymes that catalyse the synthesis of...
Includes: Supplementary data
Articles
Biochem J (2006) 399 (3): 397–404.
Published: 13 October 2006
... with presenilins. 1 To whom correspondence should be addressed (email monteiro@umbi.umd.edu ). 23 3 2006 28 6 2006 3 7 2006 3 7 2006 The Biochemical Society, London 2006 dimerization immunoprecipitation oligomerization presenilin ubiquilin yeast two-hydrid...
Articles
Biochem J (2006) 394 (1): 217–225.
Published: 27 January 2006
... oligomerization of two different proteins and a still debated number of monomers. To clarify the topology of the pore, we have mutated single residues – placed near the right and left interfaces of each monomer into cysteine. The mutants were labelled with fluorescent probes, forming a donor–acceptor pair for...
Articles
Biochem J (2005) 391 (3): 601–611.
Published: 25 October 2005
... to bound mant-GTP [where mant stands for 2′-(3′)- O -( N -methylanthraniloyl)] suggests that the G-domain and PH domain are in close proximity (5–6 nm). Promotion of dynamin-2 oligomerization, by reduction in ionic strength or increasing protein concentration, had little effect on intrinsic dynamin...
Articles
Biochem J (2005) 391 (2): 203–213.
Published: 10 October 2005
... DNA microarray histone-like nucleoid structuring protein (H-NS) oligomerization temperature regulation Pathogenic bacteria sense environmental stimuli by a range of mechanisms to ensure that virulence factors are only expressed in the correct location within the host [ 1 ]. The integrated...
Includes: Supplementary data
Articles
Biochem J (2005) 386 (1): 73–83.
Published: 08 February 2005
... channel for the passage of cytochrome c are not clear. The present study approaches this problem by addressing the oligomeric status of VDAC and its role in the induction of the permeability transition pore and cytochrome c release. Chemical cross-linking of isolated mitochondria or purified VDAC with...
Articles
Biochem J (2005) 385 (3): 625–637.
Published: 24 January 2005
... concept of GPCR oligomerization, as well as demonstrating GPCR interactions with GPCR kinases, β-arrestins, adenylate cyclase and a subunit of an inwardly rectifying K + channel. The present review examines recent technological advances and experimental applications of FRET and BRET, discussing...
Articles
Biochem J (2004) 384 (2): 255–262.
Published: 23 November 2004
...John D. DORAN; Xun LIU; Paul TASLIMI; Ahmad SAADAT; Ted FOX The effect of the length of ROCK (Rho-associated kinase) on its oligomerization state has been investigated by analysing full-length protein and four truncated constructs using light-scattering and analytical ultracentrifugation methods...
Articles
Biochem J (2004) 378 (3): 735–744.
Published: 15 March 2004
... of self-association into oligomeric structures and use nucleotide binding and hydrolysis to regulate their biological output. The Escherichia coli transcription activator PspF (phage shock protein F) is a member of the σ 54 -dependent transcriptional activators that belong to the AAA protein family...
Articles
Biochem J (2004) 377 (2): 407–417.
Published: 15 January 2004
... martin_hemler@dfci.harvard.edu ). 10 7 2003 1 10 2003 14 10 2003 14 10 2003 The Biochemical Society, London ©2004 2004 CD9 cross-linking cysteine oligomerization palmitoylation tetraspanin Abbreviations used: BMB, 1,4-bismaleimidobutane; BMH, 1,6-bis(maleimido...
Articles
Biochem J (2003) 372 (2): 347–357.
Published: 01 June 2003
... 1 2003 19 2 2003 25 2 2003 25 2 2003 The Biochemical Society, London ©2003 2003 alternative polyadenylation alternative splicing autodephosphorylation calmodulin kinase IIγ oligomerization smooth muscle Abbreviations used: CaM, calmodulin; CaMKII, CaM...
Articles
Biochem J (2003) 370 (3): 881–889.
Published: 15 March 2003
... The Biochemical Society, London ©2003 2003 copper transport membrane topology N-linked glycosylation oligomerization solute carrier Abbreviations used: BCS, bathocuproine disulphonic acid; CTR, copper transporter; ER, endoplasmic reticulum; hCTR, human CTR; ONPG, o -nitrophenyl-β...
Articles
Biochem J (2002) 368 (2): 555–563.
Published: 01 December 2002
... related to the canonical GC box and was bound by a CTIP1 oligomeric complex(es) in vitro . Furthermore, both CTIP1 and CTIP2 repressed transcription of a reporter gene harbouring a multimerized CTIP binding site, and this repression was neither reversed by trichostatin A (an inhibitor of known class I and...
Articles
Biochem J (2002) 366 (2): 423–434.
Published: 01 September 2002
... (e-mail kirsty.hewitson@chemistry.ox.ac.uk ). 21 1 2002 9 5 2002 21 5 2002 21 5 2002 The Biochemical Society, London ©2002 2002 arginase manganese enzyme oligomerization Abbreviations used: CAS, clavaminic acid synthase; CNS, crystallography and NMR...
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Biochem J (2002) 363 (3): 547–552.
Published: 24 April 2002
... translocated to mitochondria, inserts into the outer membrane, oligomerizes and triggers the release of cytochrome c , possibly by channel formation. The BH3 domain-only protein Bid induces a conformational change in Bax before its insertion into the outer membrane. The mechanism by which Bid promotes Bax...
Articles
Biochem J (2001) 356 (3): 859–866.
Published: 08 June 2001
.... 1 To whom correspondence should be addressed (e-mail mhijikat@virus.kyoto-u.ac.jp ). 29 1 2001 29 3 2001 17 4 2001 The Biochemical Society, London ©2001 2001 oligomerization p53 splicing variant transcription Abbreviations used: p53BCS, p53-binding consensus...
Articles
Biochem J (2001) 354 (3): 613–625.
Published: 08 March 2001
... higher-order oligomeric forms. To investigate this phenomenon further, homogeneous preparations of rHuAChE differing in their oligomerization statuses were generated: (1) monomers, represented by the oligomerization-impaired C580A-rHuAChE mutant, (2) wild-type (WT) dimers and (3) tetramers of WT-rHuAChE...