...Yubao Liu; Rui Zou; Zhen Wang; Chuanyang Wen; Fan Zhang; Fuqing Lin The present study was designed to explore whether exosomal lncRNA-KLF3-AS1 derived from human mesenchymal stem cells (hMSCs) can serve as a positive treatment for osteoarthritis (OA). hMSCs and MSC-derived exosomes (MSC-exo) were...

... compressive load. Proteolysis of aggrecan by members of the proteinase family ADAMTS (A disintegrin-like and metalloproteinase domain with thrombospondin type 1 motif), was identified as an early step in the inexorable destruction of cartilage in osteoarthritis (OA). Of the investigated proteinases, ADAMTS5...

... proteoglycan in the cartilage and its activity is implicated in the development of osteoarthritis (OA). To generate specific exosite inhibitors for it, we screened a phage display antibody library in the presence of the zinc-chelating active site-directed inhibitor GM6001 (Ilomastat) and isolated four highly...

... for developmental processes and promotes cartilage maturation into bone. sFRP3 is chondroprotective and is expressed in chondrocytes of healthy articular cartilage. Upon damage to cartilage, sFRP3 is down-regulated. Rare variants of sFRP3 are associated with osteoarthritis. The present study demonstrates a novel...

... in the development of OA (osteoarthritis) through its degradation of aggrecan, the major proteoglycan component of the cartilage extracellular matrix [ 1 , 2 ]. As there are currently no disease-modifying therapeutics available to treat OA, there has been considerable interest in developing ADAMTS-5 inhibitors...

... in any medium, provided the original work is properly cited. a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) aggrecanase collagenase metalloproteinase osteoarthritis tissue inhibitor of metalloproteinases 3 (TIMP-3) The TIMPs (tissue inhibitors...

...Paula J. MROZ; Jeremiah E. SILBERT GlcN (glucosamine) is now promoted over the counter for implied treatment of osteoarthritis, ostensibly by stimulating biosynthesis of cartilage chondroitin sulphate. In order to evaluate whether exogenous GlcN has any stimulatory effect, we have incubated mouse...

...Roman ZWICKY; Kathrin MÜNTENER; Mary B. GOLDRING; Antonio BAICI Cathepsin B, a marker of the dedifferentiated chondrocyte phenotype, contributes to cartilage destruction in osteoarthritis and pathological proteolysis in rheumatoid arthritis and cancer. In search of possible means for neutralizing...

... amounts of PGE # than continuous monolayer cultures (algfi) after stimulation with IL-1b. More- over, IL-1b induced COX-2 expression at 0.01 ng}ml in (alg ) INTRODUCTION Osteoarthritis (OA) results from a failure of chondrocytes within the joint to maintain the balance between synthesis and degra- dation...

... of late-stage osteoarthritis patients is remarkably similar to normal, whereas the synovial fluid aggrecan is more fragmented than that from normal or injured knees. The analyses suggest that the role of the ADAMTS and these MMP-like activities in human cartilage are distinctly different. Excessive ADAMTS...

... The Biochemical Society, London ©2001 2001 ABC lyase chondroitin sulphate endolyase glycosaminoglycan osteoarthritis Abbreviations used: CS, chondroitin sulphate; GAG, glycosaminoglycan; Gal, galactose; GalNAc, N -acetylgalactosamine; GlcA, glucuronic acid; HPAEC, high-pH anion-exchange...

... a delicate balance of biosynthetic and degra- dative processes [1]. It is now clear that accelerated degradation of aggrecan in cartilage, such as that which occurs in cytokine- stimulated cartilage explants [2 4] or in human osteoarthritis [5], is due largely to the activity of one or more aggrecanases...