...Pravin Patel; Meghna U. Naik; Kalyan Golla; Noor F. Shaik; Ulhas P. Naik Apoptosis signal-regulating kinase 1 (ASK1) is a mitogen-activated protein kinase kinase kinase (MAPKKK) that regulates activation of the c-Jun N-terminal kinase (JNK)- and p38-stress response pathways leading to apoptosis...

... was set to 1% at the peptide, protein and site level. The MaxQuant output file PhosphoSTY Sites.txt was then imported into Perseus (version 1.4.0.2). IKK NF-κB1 p38 TLR TPL-2 tumour necrosis factors Toll-like receptors (TLRs) play a central role in the initiation of innate immune...

... that GBA prevented ovariectomy-induced bone loss in a dose-dependent manner. Moreover, we demonstrated that GBA suppressed RANKL (receptor activator of nuclear factor κB ligand)-induced JNK (c-Jun N-terminal kinase), p38 and Akt phosphorylation. Taken together, our results demonstrate that GBA inhibits...

...Manoj B. Menon; Christopher Tiedje; Juri Lafera; Natalia Ronkina; Timo Konen; Alexey Kotlyarov; Matthias Gaestel The p38 MAPK (mitogen-activated protein kinase)/MK2 [MAPKAP (MAPK-activated protein) kinase-2] signalling pathway is a major regulator of stress- and cytokine-induced gene expression...

...-terminal kinase), p38 MAPK , ERK (extracellular-signal-regulated kinase) and Akt were activated, and NP202, a pro-drug of DiOHF, reduced infarct size and inhibited JNK and p38 MAPK activation, whereas ERK and Akt phosphorylation were unaltered. Similarly, in cultured myoblasts, DiOHF pre-treatment...

...-bisphosphate), a key modulator of glycolysis and gluconeogenesis. The PFKFB3 gene is involved in cell proliferation owing to its role in carbohydrate metabolism. In the present study we analysed the mechanism of regulation of PFKFB3 as an immediate early gene controlled by stress stimuli that activates the p38...

... 2011 3 10 2011 5 10 2011 5 10 2011 © The Authors Journal compilation © 2012 Biochemical Society 2012 cAMP-response-element-binding protein (CREB) mitogen-activated protein kinase (MAPK) mitogen- and stress-activated kinase (MSK) p38 ribosomal S6 kinase (RSK) MAPK...

... permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. cell signalling hypothiocyanous acid (HOSCN) mitogen-activated protein kinase (MAPK) myeloperoxidase p38 protein tyrosine phosphatase thiol 1 To whom...

...Ana Cuadrado; Angel R. Nebreda The p38 MAPK (mitogen-activated protein kinase) signalling pathway allows cells to interpret a wide range of external signals and respond appropriately by generating a plethora of different biological effects. The diversity and specificity in cellular outcomes...

... analysed the activity, regulation and phenotypic effects of mammalian MAPKs in yeast. Unexpectedly, all mammalian MAPKs tested were spontaneously phosphorylated in yeast. JNKs (c-Jun N-terminal kinases) lost their phosphorylation in pbs2 Δ cells, but p38s and ERKs (extracellular-signal-regulated kinases...

...Claire E. Mccoy; Andrew Macdonald; Nick A. Morrice; David G. Campbell; Maria Deak; Rachel Toth; Joanne Mcilrath; J. Simon C. Arthur MSK1 (mitogen- and stress-activated kinase 1) is a dual kinase domain protein that acts downstream of the ERK1/2 (extracellular-signal-regulated kinase 1/2) and p38...

... proliferation and motility. Thus the ATF3 gene can be characterized as an ‘adaptive response’ gene for the cells to cope with extra- and/or intra-cellular changes. In the present study, we demonstrate that the p38 signalling pathway is involved in the induction of ATF3 by stress signals. Ectopic expression...

...Joanne Darragh; Ana Soloaga; Victoria A. Beardmore; Andrew D. Wingate; Giselle R. Wiggin; Mark Peggie; J. Simon C. Arthur MSK (mitogen- and stress-activated protein kinase) 1 and MSK2 are kinases activated downstream of either the ERK (extracellular-signal-regulated kinase) 1/2 or p38 MAPK (mitogen...

...Claire E. McCOY; David G. CAMPBELL; Maria DEAK; Graham B. BLOOMBERG; J. Simon C. ARTHUR MSK1 (mitogen- and stress-activated protein kinase) is a kinase activated in cells downstream of both the ERK1/2 (extracellular-signal-regulated kinase) and p38 MAPK (mitogen-activated protein kinase) cascades...

...) and metformin and the p38 inhibitor, SB203580. To differentiate between a sodium-coupled (SGLT1) and diffusive (GLUT2) route of entry, fluxes were measured in the presence of the SGLT1 and GLUT2 inhibitors phloridzin and phloretin. Glucose transporter mRNA levels were measured by reverse transcriptase–PCR...

...-specificity phosphatase activity. When expressed in mammalian cells, LDP-3 protein is localized mainly to the apical submembrane area. Forced expression of LDP-3 does not alter activation of ERK (extracellular-signal-regulated kinase), but rather enhances activation of JNK (c-Jun N-terminal kinase) and p38...

... of signal pathways in MG132-induced HO-1 gene expression was examined using chemical inhibitors. Antioxidant N -acetylcysteine and SB203580, an antioxidant and inhibitor of p38 MAPK (mitogen-activated protein kinase), abolished MG132-inducible HO-1 expression. Furthermore, MG132 activated the p38 MAPK...

... controlling these processes are not well understood. To analyse the expression and roles of p38 mitogen-activated protein kinases in this process, we have established a micromass system for the reproducible hypertrophic differentiation of mouse mesenchymal limb bud cells. Our results show that all four...

... of that cascade by low levels of GPX1 activity is associated with its protection against the pro-oxidant-induced liver aponecrosis. Key words: apoptosis, oxidative stress, p38, paraquat, signalling. death of liver cells, including necrosis, apoptosis or aponecrosis, a combined process of apoptosis and necrosis...

... and phosphorylation of this site, induced by TNF-α and low (but not high) concentrations of anisomycin, is prevented by SB 203580, which inhibits SAPK2a/p38, their ‘upstream’ activator. The phosphorylation of Ser-359 induced by high concentrations of anisomycin is probably catalysed by SAPK4/p38Δ in cells, because...

... retained intrinsic tyrosine phosphatase activity. In addition, DMKP-3 specifically inhibited extracellular-signal-regulated kinase (ERK) activity, but was without a significant affect on c-Jun N-terminal kinase (JNK) and p38 activities, when it was overexpressed in Schneider cells. DMKP-3 interacted...

... examined the association of these molecules by immunoprecipitation and immunoblot analysis. The association of Pyk2 with Lyn was dependent on stimulation with MCP-1 and on tyrosine phosphorylation of Pyk2. Phosphorylation of p38 was also dependent on tyrosine phosphorylation of Pyk2. However...