... that cullin ligases also drive CMG-MCM7 ubiquitylation in human cells, thereby making the helicase into a substrate for the p97 unfoldase. Using purified human proteins, including a panel of E2 ubiquitin-conjugating enzymes, we have reconstituted CMG helicase ubiquitylation, dependent upon neddylated CUL2...

...Matthew Locke; Julia I. Toth; Matthew D. Petroski The ATPase associated with various cellular activities p97 has a critical function in the cytoplasmic degradation of proteins misfolded in the ER (endoplasmic reticulum) through a mechanism known as ERAD (ER-associated degradation). During...

... cycle 48 (Cdc48) chaperone p97 proteasome ubiquitin yeast The UPS (ubiquitin–proteasome system) constitutes the major protein degradation machinery in eukaryotic cells and regulates many essential cellular processes, including the cell cycle, signal transduction, transcription and protein...

.... In the present paper, we report that AIRAPL binds and forms a complex with p97 [VCP (valosin-containing protein)/Cdc48], Ubxd8 (ubiquitin regulatory X domain 8), Npl4–Ufd1, Derlin-1 and Bag6 on the ER (endoplasmic reticulum) membrane. In spite of the fact that AIRAPL complex partners are involved in the ERAD (ER...

... peptide N-glycanase (PNGase) p97 stress-activated protein kinase (SAPK) ubiquitin-associated domain (UBA domain) ubiquitin-like domain (UBX domain) valosin-containing protein (VCP) The human genome encodes approx. 30000 proteins and 500 protein kinases. Since approx. 30% of mammalian proteins...

...: Alzheimer s disease, immunodetection, immuno- globulin depletion, L235 antibody, melanotransferrin, p97. INTRODUCTION Mtf (melanotransferrin) or p97 was initially identified at high levels as a surface marker of malignant melanoma cells [1]. The p97 protein was later called Mtf because of its high sequence...