... in the release of cytochrome c and the commitment of apoptosis in these cells needs further investigation. Several lines of evidence, including directly patch-clamping apoptotic mitochondria, support the idea that MAC is the mediator of cytochrome c release in a variety of cell types with different...

.... Visual patch-clamp recordings were made from neurons of nucleus ambiguus in neonatal rat medullary slices. The whole-cell patch-clamp technique was similar to that described previously [ 39 ]. The hindbrain was dissected and placed in ice-cold Krebs solution (127 mM NaCl, 1.9 mM KCl, 1.2 mM KH 2 PO 4...

[email protected] ). 19 4 2006 7 7 2006 14 7 2006 14 7 2006 The Biochemical Society, London 2006 cytochrome eosinophil NADPH oxidase patch-clamp proton channel phosphoinositide The phagocyte NADPH oxidase (phox) is a transmembrane enzyme complex that translocates...

... into NAD + and fluorimetric determination of NAD + . Intracellular cADPR concentrations were approx. 0.2 μM and not altered by fMLP. In patch–clamp experiments, ADPR (0.1–100 μM) was dialysed into granulocytes to analyse its effects on whole-cell currents characteristic for TRPM2, in the presence of a low...

... not produce its intermediate isoform. Patch-clamp techniques showed that the application of 5 nM PRL increased both the macroscopic K + current amplitude and the single K + -channel open probability. This single-channel activity increase was reduced by the tyrosine kinase inhibitors genistein, herbimycin...

... Ca 2+ channels 1 and 2. TRPC6 was specific for neutrophils, whereas only in HL-60 cells were TRPC1, TRPC2, TRPC3, melastatin 1 and melastatin-related 1 found. Patch-clamp measurements in neutrophils revealed non-selective cation currents evoked by intracellular ADP-ribose and by NAD + . Both...

... (e-mail [email protected] ). 27 6 2001 13 9 2001 19 9 2001 The Biochemical Society, London ©2001 2001 calcium influx patch-clamp ryanodine receptor Abbreviations used: I crac , Ca 2+ -release-activated Ca 2+ current; IP 3 (R), inositol...

...Alexander R. HARMER; David V. GALLACHER; Peter M. SMITH cADP-ribose (cADPr) and nicotinic acid–adenine dinucleotide phosphate (NAADP) are two putative second messengers; they were first shown to stimulate Ca 2+ mobilization in sea urchin eggs. We have used the patch-clamp whole-cell technique...

...), and compare its activity with another well studied protein, hTRP3. When hTRP4 was transiently expressed in human embryonic kidney (HEK)-293 cells, basal bivalent cation permeability (barium) was increased. Whole-cell patch-clamp studies of hTRP4 expressed in Chinese hamster ovary cells revealed...

... integrity. 1 To whom correspondence should be sent (e-mail petesmif@;liv.ac.uk). 27 9 1999 13 12 1999 10 1 2000 The Biochemical Society, London © 2000 2000 Cl − current exocrine InsP 4 K + current patch-clamp Biochem. J. (2000) 347, 77 82 (Printed in Great...