... The redox state of the two-surface exposed cysteine residues in cytoglobin (Cygb) regulates the biochemical and potential physiological properties of the protein. Significant changes to ligand-binding kinetics, peroxidase activity and lipid-binding-induced structural changes are observed. cysteine...

...Tracy M. Josephs; Ian M. Morison; Catherine L. Day; Sigurd M. Wilbanks; Elizabeth C. Ledgerwood The peroxidase activity of cytochrome c may play a key role in the release of cytochrome c from the mitochondrial intermembrane space in the intrinsic apoptosis pathway. Induction of the peroxidase...

... of the G41S mutant of human cytochrome c can be explained by a higher than wild-type peroxidase activity triggered by phospholipid binding. A key complex in mitochondrial apoptosis involves cytochrome c and the phospholipid cardiolipin. In this complex cytochrome c has its native axial Met 80 ligand...

... Journal compilation © 2012 Biochemical Society 2012 crystal structure overoxidation peroxidase peroxiredoxin 4 (Prx4) In eukaryotes, disulfide bond formation driven by the Ero1/PDI (protein disulfide-isomerase) system in the ER (endoplasmic reticulum) generates H 2 O 2 , a highly reactive...

[email protected] ). 28 7 2010 16 12 2010 20 12 2010 20 12 2010 © The Authors Journal compilation © 2011 Biochemical Society 2011 cell signalling cytoglobin haemoglobin lipid peroxidase redox sensor Cygb (cytoglobin) is a recent addition to the haemoglobin superfamily...

...Alexander V. Peskin; Andrew G. Cox; Péter Nagy; Philip E. Morgan; Mark B. Hampton; Michael J. Davies; Christine C. Winterbourn Prxs (peroxiredoxins) are a ubiquitous family of cysteine-dependent peroxidases that react rapidly with H 2 O 2 and alkyl hydroperoxides and provide defence against...

... metabolic pathways that utilize trypanothione for the reduction of peroxides are being explored as potential novel therapeutic targets. In the present study we solved the X-ray crystal structure of one of the T. cruzi enzymes involved in peroxide reduction, the glutathione peroxidase-like enzyme TcGPXI ( T...

...Yongzheng Wu; Sheldon I. Feinstein; Yefim Manevich; Ibrul Chowdhury; Jhang Ho Pak; Altaf Kazi; Chandra Dodia; David W. Speicher; Aron B. Fisher Prdx6 (peroxiredoxin 6), a bifunctional protein with both GSH peroxidase and PLA 2 (phospholipase A 2 ) [aiPLA 2 (acidic calcium-independent PLA 2...

...Marcel Zamocky; Paul G. Furtmüller; Marzia Bellei; Gianantonio Battistuzzi; Johannes Stadlmann; Jutta Vlasits; Christian Obinger Phytopathogenic fungi such as the rice blast fungus Magnaporthe grisea are unique in having two catalase/peroxidase (KatG) paralogues located either intracellularly...

... in ascorbate peroxidase leads to the formation of a covalent haem–methionine linkage under oxidative conditions (i.e. on reaction with H 2 O 2 ). In the present study, spectroscopic, HPLC and mass spectrometric evidence is presented to show that covalent attachment of the haem to an engineered cysteine residue...

... for ascorbate peroxidase [ 31 ], using 600 μM ascorbate, 1 mM H 2 O 2 , and 5 μM Mb in 20 mM phosphate, pH 7, at room temperature (25 °C). Urate turnover experiments were similar to ascorbate turnover, except for the use of 500 μM urate instead of ascorbate, and 8 μM Hb instead of Mb. Turnover rates varied...

... and 0.45±0.16 μM respectively. These data indicate that PGHS-2 catalytically consumes NO during peroxidase turnover and that pro-inflammatory cytokines simultaneously upregulate NO synthesis and degradation pathways in murine macrophages. Catalytic NO consumption by PGHS-2 represents a novel interaction...

... faster than dimerization, and forms the addition product tyrosine hydroperoxide. We have explored structural requirements for hydroperoxide formation using tyrosine analogues and di- and tri-peptides. Superoxide and phenoxyl radicals were generated using xanthine oxidase, peroxidase and the respective...

... demonstrated broad substrate selectivity, its specificity constant ( V max / K m ) with phosphoenolpyruvate was >250% greater than that obtained with any other substrate. IAP exhibited significant peroxidase activity, which was optimal at pH 9.0 and insensitive to Mg 2+ or molybdate. This IAP is proposed...

...Radha CHAUHAN; Shekhar C. MANDE An alkyl hydroperoxidase (AhpC) has been found frequently to be overexpressed in isoniazid-resistant strains of Mycobacterium tuberculosis . These strains have an inactivated katG gene encoding a catalase peroxidase, which might render mycobacteria susceptible...

... cytotoxicity myeloma peroxidase Biochem. J. (1999) 344, 293 296 (Printed in Great Britain) 293 RESEARCH COMMUNICATION Amyloid-b binds catalase with high affinity and inhibits hydrogen peroxide breakdown Nathaniel G. N. MILTON1 Department of Molecular Pathology and Clinical Biochemistry, Royal Free...

... been shown here for the first time that only plant peroxidases are able to catalyse the reaction of IAA oxidation with molecular oxygen. Cytochrome c peroxidase (CcP), fungal peroxidases (manganese-dependent peroxidase, lignin peroxidase and Arthromyces ramosus peroxidase) and microperoxidase were...

...Weichi WANG; Stéphanie NOËL; Michel DESMADRIL; Jacques GUÉGUEN; Thierry MICHON The formation of a reversible adsorption complex between a dimer of N -acetyl- L -tyrosine [di-( N -acetyl-ʟ-tyrosine), (NAT) 2 ] and horseradish peroxidase (HRP) compound II (C II ) was demonstrated using a kinetic...