..., we found in a previous study that protein levels of several peroxiredoxins, including PRDX6 (peroxiredoxin 6), are highly elevated in experimentally induced melanomas. In the present study, we investigated the functional role of PRDX6 in human melanoma cells. PRDX6 is a bifunctional enzyme, which...

...Roland F. Rivera-Santiago; Sandra L. Harper; Suiping Zhou; Sira Sriswasdi; Sheldon I. Feinstein; Aron B. Fisher; David W. Speicher Peroxiredoxin-6 (PRDX6) is an unusual member of the peroxiredoxin family of antioxidant enzymes that has only one evolutionarily conserved cysteine. It reduces oxidized...

... malaria peroxiredoxin Plasmodium reactive oxygen species (ROS) Malaria, a disease caused by protozoan parasites of the genus Plasmodium and transmitted by female anopheline mosquitoes, is one of the most important infectious diseases of humans. The 2012 WHO (World Health Organization...

... electrophoresis and MS, peroxiredoxin and 15 further potentially redox-regulated proteins were identified. Among them, EhSat1 ( E. histolytica serine acetyltransferase-1), an enzyme involved in the L -cysteine biosynthetic pathway, was selected for detailed analysis. Binding of Trx to EhSat1 was verified by Far...

... the mGSH (mitochondrial glutathione) system, using the enzymes mGPx-1 (mitochondrial glutathione peroxidase-1), GRD (glutathione reductase) and mGSH, or the TRX-2 (thioredoxin-2) system, which uses the enzymes PRX-3 (peroxiredoxin-3) and TRX-2R (thioredoxin reductase-2) and TRX-2. In the present paper we...

...Andrew G. Cox; Christine C. Winterbourn; Mark B. Hampton Prxs (peroxiredoxins) are a family of proteins that are extremely effective at scavenging peroxides. The Prxs exhibit a number of intriguing properties that distinguish them from conventional antioxidants, including a susceptibility...

...Andrew G. Cox; Andree G. Pearson; Juliet M. Pullar; Thomas J. Jönsson; W. Todd Lowther; Christine C. Winterbourn; Mark B. Hampton The Prxs (peroxiredoxins) are a family of cysteine-dependent peroxidases that decompose hydrogen peroxide. Prxs become hyperoxidized when a sulfenic acid formed during...

...Yongzheng Wu; Sheldon I. Feinstein; Yefim Manevich; Ibrul Chowdhury; Jhang Ho Pak; Altaf Kazi; Chandra Dodia; David W. Speicher; Aron B. Fisher Prdx6 (peroxiredoxin 6), a bifunctional protein with both GSH peroxidase and PLA 2 (phospholipase A 2 ) [aiPLA 2 (acidic calcium-independent PLA 2...

...Svetlana N. Radyuk; Katarzyna Michalak; Vladimir I. Klichko; Judith Benes; Igor Rebrin; Rajindar S. Sohal; William C. Orr Peroxiredoxin 5 is a distinct isoform of the peroxiredoxin gene family. The antioxidative and anti-apoptotic functions of peroxiredoxin 5 have been extensively demonstrated...

...Yoshihito Iuchi; Futoshi Okada; Satoshi Tsunoda; Noriko Kibe; Nobuyuki Shirasawa; Masahito Ikawa; Masaru Okabe; Yoshitaka Ikeda; Junichi Fujii Prx (peroxiredoxin) is a multifunctional redox protein with thioredoxin-dependent peroxidase activity. Prx4 is present as a secretory protein in most...

... revealed that high-light-stress-acclimated tapx sapx double mutants compensated for the absence of tAPX and sAPX by increasing the level of 2-cysteine peroxiredoxin. Furthermore, the absence of tAPX and sAPX induced alterations in the transcriptomic profile of tapx sapx double-mutant plants already under...

...Eleanor W. Trotter; Jonathan D. Rand; Jill Vickerstaff; Chris M. Grant The yeast Tsa1 peroxiredoxin, like other 2-Cys peroxiredoxins, has dual activities as a peroxidase and as a molecular chaperone. Its peroxidase function predominates in lower-molecular-mass forms, whereas a super-chaperone form...

... disulfide bridge between Cys 47 and Cys 95 . As shown by MS, regeneration of the reduced enzyme by Tpx involves a transient mixed disulfide between Cys 95 of the peroxidase and Cys 40 of Tpx. The catalytic mechanism of the Tpx peroxidase resembles that of atypical 2-Cys-peroxiredoxins but is distinct from...

...-3 in the light under conditions of limited acceptor availability for photosynthesis as realized under environmental stress. Chloroplast Prx (peroxiredoxins) were identified as interacting partners of CYP20-3 in a DNA-protection assay. A catalytic role in the reduction of 2-Cys PrxA and 2-Cys PrxB...

...Svetlana N. RADYUK; Rajindar S. SOHAL; William C. ORR Recently, we identified a set of five genes constituting the peroxiredoxin gene family in Drosophila melanogaster [Radyuk, Klichko, Spinola, Sohal and Orr (2001) Free Radical Biol. Med. 31 , 1090–1100]. This set includes two abundant thioredoxin...