...-terminal region, an OTU-fold catalytic domain, and a ubiquitin-interacting motif (UIM) containing region. Enhanced enzymatic activity and a strong preference for K63-linked substrates are imparted by the UIM containing region. We used phage display with a ubiquitin variant (UbV) library to identify binders...

... a human antibody phage display library, which forms a distinct macromolecular complex with HtrA1 and inhibits the enzymatic activity of recombinant and native HtrA1 forms. Using biochemical methods and negative-staining EM we were able to elucidate the molecular composition of the IgG94 and Fab94...

... proteoglycan in the cartilage and its activity is implicated in the development of osteoarthritis (OA). To generate specific exosite inhibitors for it, we screened a phage display antibody library in the presence of the zinc-chelating active site-directed inhibitor GM6001 (Ilomastat) and isolated four highly...

... domain of P2X 3 using phage display. The genes encoding the scFv and activated di-chain form of BoNT/A without the C-terminal-binding subdomain (LC–H N –H CN /A) were ligated and expressed in Escherichia coli cells as a composite fusion protein. The purified protein bound and entered P2X 3 -containing...

... phage display pseudo-protease serine protease Antibodies (or immunoglobulins) are produced by immune cells in response to substances (antigens) that are recognized as foreign by the human body. The five different classes of antibodies (IgD, IgA, IgM, IgE and IgG) perform different functions...

... of the current study was to use phage display to investigate the roles played by residues near the C-termini in these interactions. Unfortunately, hsp90's terminal carboxylic group is essential for TPR binding [ 27 ]. This meant that it was not feasible to utilize many of the bacteriophages commonly used...

... the synthesized nucleotide substrate UDP-MurNAc-Ala-Glu (uridine 5′-diphosphoryl N -acetylmuramoyl- L -alanyl- D -glutamate). When coupled to a competitive bio-panning technique using a M13 phage display library encoding ∼2.7×10 9 random peptide permutations and the specific substrates meso-A2pm (meso...

... a phage-displayed peptide library with murine uPA as bait. We thereby isolated several murine uPA-binding peptide sequences, the predominant of which was the disulfide-bridged constrained sequence CPAYSRYLDC, which we will refer to as mupain-1. A chemically synthesized peptide corresponding...

... we determined that the N-terminal domain of FAK physically interacted with the N-terminal domain of p53. In the present study, using phage display, site-directed mutagenesis, pulldown and immunoprecipitation assays we localized the site of FAK binding to a 7-amino-acid region (amino acids 65–71...

... (urokinase-type and tissue-type plasminogen activator respectively), with vitronectin and with endocytosis receptors of the low-density-lipoprotein family. Understanding the significance of these interactions would be facilitated by the ability to block them individually. Using phage display, we have...

...-peptide library (Ph.D.-12 phage display peptide library kit) used in the present study was obtained from New England BioLabs. This library has a complexity of 2.7×10 9 transformants, and is based on a combinatorial library of random 12-mers fused to the minor coat protein (pIII) of the M13 phage...

...) as an example. The target gene was fragmented and cloned upstream of an antibiotic-resistance gene, in the vector pPAO2, to select for in-frame polypeptides. After removal of the antibiotic-resistance gene by Cre/Lox recombination, an antigen fragment phage display library was created and selected against...

.... In the present study, we report the successful antigenic and immunogenic mimicry of mannose-containing cell-wall compounds of M. tuberculosis by dodecamer peptides identified by phage-display technology. Using a rabbit antiserum raised against M. tuberculosis cell-surface saccharides as a target for biopanning...

... the successful mimicry of IFN-β by a peptide isolated by phage-display screening using a neutralizing anti-IFN-β monoclonal antibody. The 15-mer peptide, designated SYR6, was shown to compete with IFN-β for binding to type I IFN receptor in a concentration-dependent manner, and was shown to elicit antiviral...

...Patricia H. TANI; Joseph C. LOFTUS; Ron D. BOWDITCH Directed protein evolution, which employs a combination of random mutagenesis, phage display, and in vitro selection, was used to identify second-site suppressors of the fibronectin (Fn) cell binding domain mutation Asp 1495 Ala (RGA...

... enrich a mutant enzyme [6]. A glutathione transferase with a novel binding specificity has been selected by using phage display; unfortunately the activity of this protein was severely decreased [7]. The functional display of monomeric enzymes has opened the way to using phage fd as a vehicle to select...

... 1999 cADP-ribose ecto-enzyme phage display Biochem. J. (1999) 337, 491 496 (Printed in Great Britain) 491 Novel peptide inhibitor of ecto-ADP-ribosyl cyclase of bone marrow stromal cell antigen-1 (BST-1/CD157) Atsushi SATO*, Sumie YAMAMOTO*, Katsuhiko ISHIHARA‹, Toshio HIRANO‹ and Hisato...

.... This is the first time phage display technology has been succesfully applied for direct selection, from a large naive antibody library, of antibodies that recognize selected membrane proteins in their natural context. 1 To whom correspondence should be addressed (e-mail [email protected] ). 12 8...

...Glenn E. MORRIS; Nguyen thi MAN; Nguyen thi Ngoc HUYEN; Alexander PEREBOEV; John KENDRICK-JONES; Steven J. WINDER Monoclonal antibody (mAb) binding sites in the N-terminal actin-binding domain of utrophin have been identified using phage-displayed peptide libraries, and the mAbs have been used...