...Alyssa X. Wu-zhang; Alexandra C. Newton PKC (protein kinase C) has been in the limelight since the discovery three decades ago that it acts as a major receptor for the tumour-promoting phorbol esters. Phorbol esters, with their potent ability to activate two of the three classes of PKC isoenzymes...

... were measured. These mutants showed reduced binding affinities for phorbol ester [PDBu (phorbol 12,13-dibutyrate)] and diacylglycerol [DOG ( sn -1,2-dioctanoylglycerol), SAG ( sn -1-stearoyl 2-arachidonyl glycerol)]. All five full-length PKCθ mutants exhibited reduced phorbol-ester-induced membrane...

... phosphatidylcholine; 1,2-dipalmitoyl- sn -glycero-3-phosphorylcholine) as well as in the remodelling of PC through the deacylation–reacylation pathway by lung granular pneumocytes (alveolar epithelial type II cells, AECII) [ 9 , 13 – 15 ]. Phorbol esters (e.g. phorbol myristic acid, PMA) are known surfactant...

...) hispidin phorbol ester protein kinase C (PKC) receptor for activated C-kinase 1 (RACK1) translation initiation Translation requires at least 11 eIFs (eukaryotic initiation factors) controlling the rate and accuracy [ 1 ]. The global initiation rate is controlled by signalling on the eIF4F...

... by PMA. A previously identified phorbol-ester-resistant mutant (K422E) displaying an acidic substitution in a regulatory site, exhibits constitutive traffic but, in contrast with the wild-type, fails to show glycine uptake inhibition, membrane raft redistribution and trafficking modulation by PMA. We...

...Emma Villa-Moruzzi Ser 910 of FAK (focal adhesion kinase) was phosphorylated in fibroblasts treated with the phorbol ester PMA and dephosphorylated by PP1δ (protein phosphatase 1δ), as indicated by shRNA (small-hairpin RNA) gene silencing. Ser 910 of FAK was reported previously to be an ERK...

... domain eliminates its contribution to membrane binding. Figure 1 C1 domain sequences ( A ) The C1 domains of the RasGRPs are compared with four C1 domains of known structure. The C1b domain of PKCδ and the C1 domain of β2-chimaerin (B2-chim.) bind DAG and phorbol esters, while the C1 domains of Raf...

...Chengfeng Yang; Marcelo G. Kazanietz Chimaerins are the only known RhoGAPs (Rho GTPase-activating proteins) that bind phorbol ester tumour promoters and the lipid second messenger DAG (diacylglycerol), and show specific GAP activity towards the small GTPase Rac. This review summarizes our knowledge...

...Ignacio Rubio; Knut Rennert; Ute Wittig; Katrin Beer; Matthias Dürst; Stacey L. Stang; Jim Stone; Reinhard Wetzker Ras is a major mediator of PE (phorbol ester) effects in mammalian cells. Various mechanisms for PE activation of Ras have been reported [Downward, Graves, Warne, Rayter and Cantrell...

... Sevilla, Spain. 2 To whom correspondence should be addressed (email [email protected] ). 11 7 2005 9 9 2005 9 9 2005 9 9 2005 The Biochemical Society, London 2005 cAMP cytoskeleton 14-3-3 protein phorbol ester phosphodiesterase 3A (PDE3A) protein...

... in rat renal and rat intestinal epithelial cells have shown that the tumour-promoting phorbol ester PMA potentiates the effect of 1,25D in the up-regulation of the CYP24 promoter and that this synergistic effect corresponds to an increase in CYP24 mRNA levels [ 23 – 27 ]. PMA has traditionally been used...

...Kelley K. KININGHAM; Chotiros DAOSUKHO; Daret K. ST. CLAIR Cytokines, phorbol esters, radiation and chemotherapeutic drugs up-regulate the expression of MnSOD (manganese superoxide dismutase). Using the VA-13 cell line, we studied the regulation of SOD2 upon treatment with PMA. Pre-treatment...

..., University of Utrecht, The Netherlands (email [email protected] ). 13 5 2004 14 7 2004 13 8 2004 13 8 2004 The Biochemical Society, London 2004 glucose uptake myristoylated protein kinase Cβ peptide inhibitor phorbol ester protein kinase C (PKC) 3T3-L1 adipocyte...

... 2004 23 6 2004 1 7 2004 1 7 2004 The Biochemical Society, London 2004 diacylglycerol kinase pleckstrin homology domain phorbol ester protein kinase C translocation We have found that, in addition to the translocation, endogenous or transiently expressed DGKδ1...

... cytidylyltransferase, diacylglycerol, ethanolaminephosphotransferase, McArdle- RH7777, phorbol ester, phosphatidylethanolamine. INTRODUCTION PtdEtn (phosphatidylethanolamine) is a major membrane consti- tuent, being the second most abundant phospholipid class in most mammalian tissues and cell types and often...

...Maria Jose CALOCA; HongBin WANG; Marcelo G. KAZANIETZ The regulation and function of β2-chimaerin, a novel receptor for the phorbol ester tumour promoters and the second messenger DAG (diacylglycerol), is largely unknown. As with PKC (protein kinase C) isoenzymes, phorbol esters bind to β2...

... or forskolin. These results provide insights into the factors and mechanisms that regulate expression of the hPDE7A gene. Key words: cAMP, cAMP-response-element-binding protein (CREB), CpG island, phorbol ester, transcription. INTRODUCTION Signalling by cyclic nucleotides is a major signal-transduction pathway...

... (Saale), Germany (e-mail [email protected] ). 3 1 2003 7 2 2003 11 3 2003 11 3 2003 The Biochemical Society, London ©2003 2003 Ets protein protein kinase C parathyroid hormone-related protein phorbol ester mitogen-activated protein kinase...

..., London ©2001 2001 ERK/MAP kinase phorbol ester PKC inhibitors metalloproteinase Abbreviations used: ADAM, a disintegrin and metalloproteinase; ERK, extracellular signal-regulated protein kinase; FCS, fetal calf serum; IL, interleukin; MAPK, mitogen-activated protein kinase; MFR, mean...

... responded poorly to phorbol ester activation. However, using several analytical techniques, we found no evidence that the somatic ACE cleavage site differed from that characterized in testis ACE. First, anti-peptide antibodies raised to specific sequences on either side of the reported cleavage site (Arg...

.... The effect of activation was sensitive to the antioxidant enzymes superoxide dismutase and catalase. When ascorbate was oxidized in situ by chemical or enzymic oxidation, the rates of uptake were similar to those after activation of the cells by phorbol ester; however, in the latter case the extracellular...

...Iris AUGUSTIN; Andrea BETZ; Claudia HERRMANN; Tobias JO; Nils BROSE Munc13-1, a mammalian homologue of Caenorhabditis elegans unc-13p, is a presynaptic phorbol ester receptor that enhances neurotransmitter release. In the present study we analysed the regional, cellular and subcellular expression...

... on PKC, but that the extent to which PLD becomes activated depends largely, if not entirely, on the magnitude and duration of the agonist-induced increase in [Ca i . Key words: chelerythrine, phorbol ester, phospholipase D, staurosporine, thapsigargin. reaction [7]. To date, the physiological relevance...