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Keywords: photoaffinity
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Articles

Photoaffinity labelling displacement assay using multiple recombinant protein domains

Open Access
In Collection
Awards
David J. Fallon, Alex Phillipou, Christopher J. Schofield, David House, Nicholas C. O. Tomkinson, Jacob T. Bush
Journal: Biochemical Journal
Biochem J (2023) 480 (15): 1183–1197.
https://doi.org/10.1042/BCJ20230129
Published: 31 August 2023
...David J. Fallon; Alex Phillipou; Christopher J. Schofield; David House; Nicholas C. O. Tomkinson; Jacob T. Bush The development and optimisation of a photoaffinity labelling (PAL) displacement assay is presented, where a highly efficient PAL probe was used to report on the relative binding...
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Includes: Supplementary data
Articles

PKCϵ has an alcohol-binding site in its second cysteine-rich regulatory domain

Available to Purchase
Joydip Das, Satyabrata Pany, Ghazi M. Rahman, Simon J. Slater
Journal: Biochemical Journal
Biochem J (2009) 421 (3): 405–413.
https://doi.org/10.1042/BJ20082271
Published: 15 July 2009
... alcohol-binding site alcoholism fluorescence spectroscopy mass spectrometry (MS) photoaffinity protein kinase Cϵ (PKCϵ) Alcoholism and alcohol abuse causes major health problems worldwide. Alcohol is casually related to more than 60 different medical conditions. Overall 4% of the global...
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Includes: Supplementary data