...Kalanethee PAUL-PLETZER; Takeshi YAMAMOTO; Noriaki IKEMOTO; Leslie S. JIMENEZ; Hiromi MORIMOTO; Philip G. WILLIAMS; Jianjie MA; Jerome PARNESS Dantrolene is an inhibitor of intracellular Ca 2+ release from skeletal muscle SR (sarcoplasmic reticulum). Direct photoaffinity labelling experiments using...

..., Japan. 2 To whom correspondence should be addressed (email [email protected] ). 8 4 2004 24 6 2004 28 6 2004 28 6 2004 glycoprotein legume lectin photoaffinity labelling Sophora japonica (Japanese pagoda tree) bark self-aggregation vacuole A large...

... were then incubated for an additional 10 min on ice followed by UV irradiation at 254 nm for 10 min while on ice (Stratgene 1800 UV cross-linker; Stratagene, La Jolla, CA, U.S.A.) [ 14 ]. Following photoaffinity labelling, samples were mixed with 50 μl of buffer A (1% SDS and 0.05 M Tris, pH 7.4...

... Society, London 2004 atherosclerosis CD36 growth hormone-releasing peptide hexarelin oxidized low-density lipoprotein photoaffinity labelling The GHRPs (growth hormone-releasing peptides) comprise a family of small synthetic peptides derived from Met-enkephalin that demonstrate potent...

...-coupled receptor p -benzoyl- l -phenylalanine peptide mapping photoaffinity labelling Abbreviations used: AngII, angiotensin II; Bz-Phe, p -benzoyl- l -phenylalanine; DMEM, Dulbecco's modified Eagle's medium; ECL, extracellular loop; Endo Arg-C, endoproteinase Arg-C; ET-1, endothelin-1; GPCR...

...Kohichi KAWAHARA; Akihiko KUNIYASU; Katsuyoshi MASUDA; Masaji ISHIGURO; Hitoshi NAKAYAMA To identify photoaffinity-labelled amino acid residue(s), we devised an effective method utilizing immunoaffinity purification of photolabelled fragments, followed by matrix-assisted laser-desorption ionization...

... revealed that only the 62kDa form of the enzyme is present in vivo , but we utilized the proteolytic sensitivity of FadD to investigate its structure. Photoaffinity labelling experiments revealed that both intact FadD and the 43kDa fragment bound a long-chain fatty acid. Intact and cleaved FadD were also...

... photoaffinity label, [ 125 I]iodoaminoflisopolol ([ 125 I]IAmF). A unique property of this photolabel is that the photoreactive centre is also the binding pharmacophore, which corresponds to the catechol end of related β 2 AR agonists. [ 125 I]IAmF specifically photolabels membrane-bound and purified β 2 AR...