... by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . αB-crystallin Hsp27 molecular chaperones photobleaching proteostasis single-molecule fluorescence Small heat shock proteins (sHsps) are a ubiquitously...

... complex. The method relies both on sensitized acceptor emission and on donor de-quenching (by photobleaching of the acceptor molecules), coupled with full spectral analysis of the differential fluorescence signature, in order to quantify the donor/acceptor energy transfer. The approach and sensitivity...

... with FITC-labelled β-enolase in the presence or absence of free CK. A small amount of bound β-enolase was observed in the presence of large concentrations of CK. The mobility of enolase was measured in cultured satellite cells by modulated-fringe-pattern photobleaching. FITC-labelled β-enolase was totally...