... of the active state raises the question of how serpins fold, whereas the misfolding of some serpin variants that leads to polymerization and pathologies of liver disease, emphysema and dementia makes it clinically important to understand how such polymerization might occur. Finally, since binding of serpins...

... of palladin on actin dynamics has remained elusive. In the present study, we show that the actin-binding immunoglobulin-like domain of palladin, which is directly responsible for both actin binding and bundling, also stimulates actin polymerization in vitro . Palladin eliminated the lag phase...

... activity of their cognate catalytic domains by increasing enzyme substrate proximity. Mimicking their role in Nature, we, in the present study, propose that CBMs may assist in vitro glycosynthase-catalysed polymerization reactions to produce artificial polysaccharides. Glycosynthases are GHs that have been...

... transition to the polymerization intermediate, and which persists in the ensuing oligomer. This epitope is not shared by loop-inserted monomeric conformations. We show the increased amenity to polymerization by either the pathogenic E342K mutation or the binding of mAb 5E3 occurs without affecting...

..., which can result in a growing polymer chain of inactive molecules. Different types of polymer are possible, but, experimentally only heat has been shown to generate polymers in vitro consistent with ex vivo pathological specimens. Many mutations that alter the rate of heat-induced polymerization have...

[email protected] ). actin depolymerizing factor (ADF)/cofilin actin dynamics cyclase-associated protein (CAP) depolymerization nucleotide exchange polymerization Modulation of actin filament turnover is critical for many cell biological events. Actin by itself can undergo turnover or treadmilling...

... loss of SH groups leads to progressive increases in the critical concentration and loss of polymerization competence that can be reversed by assembly promoters such as higher protein concentration, taxol or high ionic strength. Under such conditions, the substituted tubulin forms protofilament-based...

... by native gel electrophoresis, the size of the PAI-1 polymers ranged from dimers to multimers of more than 20 units. As compared with native PAI-1, the polymers exhibited an increased resistance to temperature-induced unfolding. Polymerization was associated with specific changes in patterns of digestion...

... confirmed by surface-plasmon-resonance assays demonstrating moderate affinities ( K d = 0.02 to > 6 μ M) for the binding to laminin-1. This indicated that laminins containing α3B or α5 chains should also be able to form non-covalent networks by polymerization. The LN modules also showed heparin binding...

... 1 3 2000 15 3 2000 The Biochemical Society, London © 2000 2000 polymerization retrotransposon sequence comparison template-primer yeast. Biochem. J. (2000) 38, 337 342 (Printed in Great Britain) 337 Expression of an active form of recombinant Ty1 reverse transcriptase...

... temperatures and long reaction times. Here we show that certain widely used reaction conditions, involving very high concentrations of acetic acid/acetate mixtures, do not produce substantial amounts of polymeric β-haematin on immediate isolation of the reaction products, but only during prolonged drying...