... Lipids. Cholesterol was purchased from Sigma–Aldrich. apoptosis Bcl-2 dipole mitochondria planar membrane pore Recombinant full-length Bax was purified as reported previously [ 8 , 12 ]. Only the soluble protein was recovered from the bacterial lysate leaving any inclusion bodies...

..., substrates and intermediates. Recent structural studies of bacterial microcompartment proteins have revealed both hexamers and pentamers that assemble to generate the pseudo-icosahedral bacterial microcompartment shell. Some of these shell proteins have pores on their symmetry axes. Here we report...

... by electrospray MS. Using a liposome glucose-release assay, the membrane-inserted structure was seen to retain its function as a membrane pore even after removal of exposed N-terminal segments by proteolysis. These data strongly suggest that the pores for glucose release are assembled from the three major β...