... expression. Lipophilic molecules were extracted from rat livers, saponified and re-constituted as an LE (lipophilic extract). LE synergized with insulin to induce primary hepatocyte, but not β-cell, Gck expression in an SREBP-1c (sterol-regulatory-element-binding protein-1c)-independent manner. The dramatic...

... manner, and this DR-1 is required for full glucose induction of the FAS promoter in primary hepatocytes. In addition, a 3-fold knockdown of hepatocyte HNF-4α by small interfering RNA produces a corresponding decrease in FAS gene induction by glucose. Co-immunoprecipitation experiments demonstrate...

[email protected] ). The methods for hepatocyte isolation and culture in serum-free medium, essentially those of Waxman et al. [ 6 ], as well as those for liposome-mediated transfection [ 25 ] and plasmid purification, were as described previously [ 14 ]. Typically, for transfection of primary hepatocytes...