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Keywords: promoter
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Articles
The cooperation of cis -elements during M-cadherin promoter activation
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2021) 478 (4): 911–926.
Published: 26 February 2021
...) during terminal myogenic differentiation. Several conserved cis -elements, including 5 E-boxes, 2 GC boxes, and 1 conserved downstream element (CDE) were identified in the M-cadherin proximal promoter. We found that E-box-3 and -4 close to the transcription initiation site (TIS) mediated most of its...
Includes: Supplementary data
Articles
Investigation of specific interactions between T7 promoter and T7 RNA polymerase by force spectroscopy using atomic force microscope
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Journal:
Biochemical Journal
Biochem J (2018) 475 (1): 319–328.
Published: 11 January 2018
...Xiaojuan Zhang; Zhixuan Yao; Yanting Duan; Xiaomei Zhang; Jinsong Shi; Zhenghong Xu The specific recognition and binding of promoter and RNA polymerase is the first step of transcription initiation in bacteria and largely determines transcription activity. Therefore, direct analysis...
Articles
Transcription factors YY1, Sp1 and Sp3 modulate dystrophin Dp71 gene expression in hepatic cells
Available to PurchaseKatia Peñuelas-Urquides, Carolina Becerril-Esquivel, Laura C. Mendoza-de-León, Beatriz Silva-Ramírez, José Dávila-Velderrain, Bulmaro Cisneros, Mario Bermúdez de León
Journal:
Biochemical Journal
Biochem J (2016) 473 (13): 1967–1976.
Published: 28 June 2016
... aimed at identifying DNA elements and transcription factors involved in Dp71 expression in hepatic cells. Relevant DNA elements on the Dp71 promoter were identified by comparing Dp71 5′-end flanking regions between species. The functionality of these elements was demonstrated by site-directed...
Articles
Kinetics of promoter escape by bacterial RNA polymerase: effects of promoter contacts and transcription bubble collapse
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Journal:
Biochemical Journal
Biochem J (2014) 463 (1): 135–144.
Published: 08 September 2014
...Je Ko; Tomasz Heyduk Promoter escape by RNA polymerase, the transition between the initiation and elongation, is a critical step that defines transcription output at many promoters. In the present study we used a real-time fluorescence assay for promoter melting and escape to study the determinants...
Includes: Supplementary data
Articles
Mechanisms of transcriptional regulation by WT1 (Wilms’ tumour 1)
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Journal:
Biochemical Journal
Biochem J (2014) 461 (1): 15–32.
Published: 13 June 2014
...–epithelial transition). 3 12 2013 10 4 2014 24 4 2014 © The Authors Journal compilation © 2014 Biochemical Society 2014 co-activator co-repressor promoter transcription Wilms’ tumour 1 (WT1) zinc finger The WT1 (Wilms’ tumour 1) protein was among the first tumour...
Articles
Synthetic retinoid Am80 up-regulates apelin expression by promoting interaction of RARα with KLF5 and Sp1 in vascular smooth muscle cells
Available to PurchaseXin-rui Lv, Bin Zheng, Shu-ya Li, Ai-li Han, Chang Wang, Jian-hong Shi, Xin-hua Zhang, Yan Liu, Yong-hui Li, Jin-kun Wen
Journal:
Biochemical Journal
Biochem J (2013) 456 (1): 35–46.
Published: 24 October 2013
... markedly up-regulated apelin mRNA and protein levels in VSMCs. Furthermore, KLF5 (Krüppel-like factor 5) and Sp1 (stimulating protein-1) co-operatively mediated Am80-induced apelin expression through their direct binding to the TCE (transforming growth factor-β control element) on the apelin promoter...
Includes: Supplementary data
Articles
Nuclear factor κB down-regulates human UDP-glucuronosyltransferase 1A1: a novel mechanism involved in inflammation-associated hyperbilirubinaemia
Available to PurchaseTzu-Yue Shiu, Tien-Yu Huang, Shih-Ming Huang, Yu-Lueng Shih, Heng-Cheng Chu, Wei-Kuo Chang, Tsai-Yuan Hsieh
Journal:
Biochemical Journal
Biochem J (2013) 449 (3): 761–770.
Published: 09 January 2013
... regulatory elements in the upstream promoter region of the human UGT1A1 gene were determined using EMSA (electrophoretic mobility-shift assay) and ChIP (chromatin immunoprecipitation). The important role of the transcription regulatory element was examined using a luciferase assay, and was determined by qRT...
Articles
The human NUPR1/P8 gene is transcriptionally activated by transforming growth factor β via the SMAD signalling pathway
Available to PurchaseRoxane M. Pommier, Johann Gout, David F. Vincent, Carla E. Cano, Bastien Kaniewski, Sylvie Martel, Jonathan Rodriguez, Geneviève Fourel, Ulrich Valcourt, Julien C. Marie, Juan L. Iovanna, Laurent Bartholin
Journal:
Biochemical Journal
Biochem J (2012) 445 (2): 285–293.
Published: 27 June 2012
... was activated by TGFβ at the transcriptional level. We show that this activation is mediated by the SMAD proteins, which are transcription factors specifically involved in the signalling of TGFβ superfamily members. NUPR1 promoter analysis reveals the presence of a functional TGFβ-response element binding...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2012) 443 (3): 857–867.
Published: 16 April 2012
... that TNFα (tumour necrosis factor α) stimulation induces p110δ expression in human ECs (endothelial cells) and synovial fibroblasts, but not in leucocytes, through transcription start sites located in a novel promoter region in the p110δ gene ( PIK3CD ). This promoter is used in all cell types, including...
Includes: Supplementary data
Articles
Transforming growth factor β1 represses proximal tubular cell microRNA-192 expression through decreased hepatocyte nuclear factor DNA binding
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Journal:
Biochemical Journal
Biochem J (2012) 443 (2): 407–416.
Published: 27 March 2012
.... We found that TGF-β1 (transforming growth factor β1) down-regulates miR-192 and miR-194, co-transcribed in the shared precursor pri-miR (primary miR transcript)-192/194. Luciferase reporter analysis showed constitutive promoter activity within nucleotides +21 to −223. We identified HNF (hepatocyte...
Includes: Supplementary data
Articles
Transcriptional regulation mechanisms of hypoxia-induced neuroglobin gene expression
Available to PurchaseNing Liu, Zhanyang Yu, Shuanglin Xiang, Song Zhao, Anna Tjärnlund-Wolf, Changhong Xing, Jian Zhang, Xiaoying Wang
Journal:
Biochemical Journal
Biochem J (2012) 443 (1): 153–164.
Published: 14 March 2012
... of hypoxia. In the present study, we located the core proximal promoter of the mouse Ngb gene to a 554 bp segment, which harbours putative conserved NF-κB (nuclear factor κB)- and Egr1 (early growth-response factor 1) -binding sites. Overexpression and knockdown of transcription factors p65, p50, Egr1 or Sp1...
Articles
Unusual organization, complexity and redundancy at the Escherichia coli hcp-hcr operon promoter
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Journal:
Biochemical Journal
Biochem J (2010) 430 (1): 61–68.
Published: 28 July 2010
...David L. Chismon; Douglas F. Browning; Gregory K. Farrant; Stephen J. W. Busby Expression from the Escherichia coli hcp - hcr operon promoter is optimally induced during anaerobic conditions in the presence of nitrite. This expression depends on transcription activation by FNR (fumarate and nitrate...
Articles
Sterol-regulatory-element-binding protein 2 and nuclear factor Y control human farnesyl diphosphate synthase expression and affect cell proliferation in hepatoblastoma cells
Available to PurchaseKenji Ishimoto, Keisuke Tachibana, Ikuko Hanano, Daisuke Yamasaki, Hiroki Nakamura, Megumi Kawai, Yasuomi Urano, Toshiya Tanaka, Takao Hamakubo, Juro Sakai, Tatsuhiko Kodama, Takefumi Doi
Journal:
Biochemical Journal
Biochem J (2010) 429 (2): 347–357.
Published: 28 June 2010
... site in the human FDPS promoter. Using a luciferase assay, electrophoretic mobility-shift assay and chromatin immunoprecipitation assay, we demonstrated that these elements are responsible for the transcription of the FDPS gene, and that its transcriptional activation is mediated by SREBP-2 (sterol...
Includes: Supplementary data
Articles
Genomic organization and regulation of the human orexin (hypocretin) receptor 2 gene: identification of alternative promoters
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Journal:
Biochemical Journal
Biochem J (2010) 427 (3): 377–390.
Published: 14 April 2010
... different translational efficiencies, which regulate the level of protein expression. In the present study, we have demonstrated that the hOX2R gene is regulated by two promoters and the novel transcripts are regulated by the distal promoter located upstream of exon 1A. We have demonstrated that the AP-1...
Includes: Supplementary data
Articles
The human HIF (hypoxia-inducible factor)- 3 α gene is a HIF-1 target gene and may modulate hypoxic gene induction
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Journal:
Biochemical Journal
Biochem J (2009) 424 (1): 143–151.
Published: 23 October 2009
... is a specific target gene of HIF-1α, but is not affected by HIF-2 α knockdown. In contrast with HIF-1α and HIF-2α, HIF-3α is not regulated at the level of protein stability. HIF-3α protein could be detected under normoxia in the cytoplasm and nuclei, but increased under hypoxic conditions. Promoter analyses...
Includes: Supplementary data
Articles
A novel p53 target gene, S100A9 , induces p53-dependent cellular apoptosis and mediates the p53 apoptosis pathway
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Journal:
Biochemical Journal
Biochem J (2009) 422 (2): 363–372.
Published: 13 August 2009
... previous work showed that S100A9 was severely down-regulated in human ESCC (oesophageal squamous cell carcinoma). To further investigate the transcriptional regulation of S100A9, we analysed the S100A9 promoter region and found several putative p53BS (p53-binding sites). Luciferase reporter assays showed...
Includes: Supplementary data
Articles
KLF4 positively regulates human ghrelin expression
Available to PurchaseHyo Jung Lee, Young Mi Kang, Chang Suk Moon, Myung Kuk Joe, Joo Hyun Lim, Young Ho Suh, Jihyun Song, Myeong Ho Jung
Journal:
Biochemical Journal
Biochem J (2009) 420 (3): 403–411.
Published: 27 May 2009
..., suggesting that ghrelin expression is associated with KLF4. Then, we investigated the effects of KLF4 on the human ghrelin -promoter activity and identified a KLF4-responsive region in the promoter. KLF4 expression specifically stimulated human ghrelin -promoter activity in a dose-dependent manner in human...
Articles
Competition between NarL-dependent activation and Fis-dependent repression controls expression from the Escherichia coli yeaR and ogt promoters
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Journal:
Biochemical Journal
Biochem J (2009) 420 (2): 249–257.
Published: 13 May 2009
...Derrick J. P. Squire; Meng Xu; Jeffrey A. Cole; Stephen J. W. Busby; Douglas F. Browning The Escherichia coli NarL protein is a global gene regulatory factor that activates transcription at many target promoters in response to nitrate and nitrite ions. Although most NarL-dependent promoters...
Articles
Regulation of Jumonji-domain-containing histone demethylases by hypoxia-inducible factor (HIF)-1α
Available to PurchasePatrick J. Pollard, Christoph Loenarz, David R. Mole, Michael A. McDonough, Jonathan M. Gleadle, Christopher J. Schofield, Peter J. Ratcliffe
Journal:
Biochemical Journal
Biochem J (2008) 416 (3): 387–394.
Published: 26 November 2008
... To whom correspondence should be addressed (email [email protected] ). 18 6 2008 14 8 2008 19 8 2008 19 8 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 histone demethylase hypoxia hypoxia-inducible factor 1 (HIF-1) Jumonji domain promoter...
Includes: Supplementary data
Articles
Transcriptional regulation of the TGF -β 1 promoter by androgen receptor
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Journal:
Biochemical Journal
Biochem J (2008) 416 (3): 453–462.
Published: 26 November 2008
... prostate cancer cells. Here, we examined regulation of human TGF -β 1 promoter activity by androgen in prostate cancer cells. The full-length (−3363 to +110) promoter showed a high level of activity in response to androgen in PC3mm2 cells expressing AR (androgen receptor). Further deletion analysis...
Articles
p53 regulates its own activator: transcriptional co-activator PC4 , a new p53-responsive gene
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Journal:
Biochemical Journal
Biochem J (2007) 406 (3): 437–444.
Published: 29 August 2007
... PC4 is a unique activator of p53 function. In the present study, we report that PC4 is a p53-inducible gene. Bioinformatics analysis reveals multiple p53-binding sites in the PC4 promoter. We have found that indeed p53 binds to all the identified sites in vitro and in vivo with varying affinities. p53...
Includes: Supplementary data
Articles
Alternative promoters and repetitive DNA elements define the species-dependent tissue-specific expression of the FMO1 genes of human and mouse
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Journal:
Biochemical Journal
Biochem J (2007) 406 (3): 491–499.
Published: 29 August 2007
... the basis of this species-dependent tissue-specific transcription of FMO1 . Our results indicate the use of three alternative promoters. Transcription of the gene in fetal human and adult mouse liver is exclusively from the P0 promoter, whereas in extra-hepatic tissues of both species, P1 and P2 are active...
Articles
Insulin activates human sterol-regulatory-element-binding protein-1c (SREBP-1c) promoter through SRE motifs
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Journal:
Biochemical Journal
Biochem J (2006) 400 (1): 179–188.
Published: 27 October 2006
...Nicolas Dif; Vanessa Euthine; Estelle Gonnet; Martine Laville; Hubert Vidal; Etienne Lefai In the present study, we aimed to decipher the mechanisms involved in the transcriptional effect of insulin on the SREBP-1c specific promoter of the human srebf - 1 gene. Using luciferase reporter gene...
Articles
Dual promoters control the cell-specific expression of the human cell death-inducing DFF45-like effector B gene
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Journal:
Biochemical Journal
Biochem J (2006) 393 (3): 779–788.
Published: 13 January 2006
... that a mechanism exists for the tissue- and cell-specific regulation of transcription of this gene. We have analysed the promoters of the human CIDE - B gene, particularly the mechanism of cell-specific transcription. Expression of CIDE - B is driven by two promoters which are responsible for the synthesis of two...
Articles
Identification and analysis of the promoter region of the human methionine sulphoxide reductase A gene
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Journal:
Biochemical Journal
Biochem J (2006) 393 (1): 321–329.
Published: 12 December 2005
... of oxidative damage to cells. To understand mechanisms of human msrA gene expression and regulation, we cloned and characterized the 5′ promoter region of the human msrA gene. Using 5′-RACE (rapid amplification of cDNA ends) analysis of purified mRNA from human cells, we located the transcription initiation...
Articles
Functional analysis of the promoter of the mitochondrial phosphate carrier human gene: identification of activator and repressor elements and their transcription factors
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Journal:
Biochemical Journal
Biochem J (2005) 391 (3): 613–621.
Published: 25 October 2005
... transcriptional initiation site and lacks a TATA box. Through deletion analysis of the −1213/−25 nt region, we identified an activation domain (−223/−25) and an inhibition domain (−1017/−814). The most effective promoter activity in transfected HeLa cells corresponded to the region containing putative binding...
Articles
Regulation of the poly(ADP-ribose) polymerase-1 gene expression by the transcription factors Sp1 and Sp3 is under the influence of cell density in primary cultured cells
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Journal:
Biochemical Journal
Biochem J (2005) 389 (2): 423–433.
Published: 05 July 2005
... gene promoter is mainly dictated through its recognition by the transcription factors Sp1 and Sp3 (where Sp is specificity protein). In the present study, we investigated whether (i) both PARP-1 expression and PARP-1 enzymatic activity are under the influence of cell density in primary cultured cells...
Articles
Characterization of the promoters of Epsilon glutathione transferases in the mosquito Anopheles gambiae and their response to oxidative stress
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Journal:
Biochemical Journal
Biochem J (2005) 387 (3): 879–888.
Published: 26 April 2005
...-susceptible individuals. At least one of the eight Epsilon GSTs in this species, GSTe2, efficiently metabolizes DDT to DDE [1,1-dichloro-2,2-bis-( p -chlorophenyl)ethane]. In the present study, we investigated the factors regulating expression of this class of GSTs. The activity of the promoter regions...
Articles
Genomic organization of human arylamine N -acetyltransferase Type I reveals alternative promoters that generate different 5′-UTR splice variants with altered translational activities
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Journal:
Biochemical Journal
Biochem J (2005) 387 (1): 119–127.
Published: 22 March 2005
... according to the location of the first exon, which suggested that NAT1 expression occurs from three alternative promoters. Using RT (reverse transcriptase)-PCR, we identified one major transcript in various epithelial cells derived from different tissues. In contrast, multiple transcripts were observed...
Includes: Supplementary data
Articles
Regulation of human methylthioadenosine phosphorylase gene by the CBF (CCAAT binding factor)/NF-Y (nuclear factor-Y)
Available to PurchaseYuwaraj KADARIYA, Kaname NAKATANI, Junji NISHIOKA, Takahiko FUJIKAWA, Warren D. KRUGER, Tsutomu NOBORI
Journal:
Biochemical Journal
Biochem J (2005) 387 (1): 175–183.
Published: 22 March 2005
... of the transcriptional regulation of the MTAP gene, we have cloned the 1.29 kb fragment of the hMTAP promoter and identified cis -acting regulatory sequences using a luciferase reporter gene assay. Maximal promoter activity was associated with sequences between −446 and −152, where two CCAAT elements were located...
Articles
The expression of the human neuronal α3 Na + ,K + -ATPase subunit gene is regulated by the activity of the Sp1 and NF-Y transcription factors
Available to PurchaseRoberta BENFANTE, Ruth Adele ANTONINI, Monica VACCARI, Adriano FLORA, Fabian CHEN, Francesco CLEMENTI, Diego FORNASARI
Journal:
Biochemical Journal
Biochem J (2005) 386 (1): 63–72.
Published: 08 February 2005
... is strictly required in excitable tissues. In the present study, we used structural, biochemical and functional criteria to analyse the transcriptional mechanisms controlling the expression of the human gene in neurons, and identified a minimal promoter region of approx. 100 bp upstream of the major...
Includes: Supplementary data
Articles
A simple promoter containing two Sp1 sites controls the expression of sterol-regulatory-element-binding protein 1a (SREBP-1a)
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Journal:
Biochemical Journal
Biochem J (2005) 386 (1): 161–168.
Published: 08 February 2005
...Chengkang ZHANG; Dong-Ju SHIN; Timothy F. OSBORNE The mammalian gene for SREBP-1 (sterol-regulatory-element-binding protein 1) contains two promoters that control the production of two proteins, SREBP-1a and -1c, and each contains a unique N-terminal transcriptional activation domain...
Articles
Insulin activates the rat sterol-regulatory-element-binding protein 1c (SREBP-1c) promoter through the combinatorial actions of SREBP, LXR, Sp-1 and NF-Y cis -acting elements
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Journal:
Biochemical Journal
Biochem J (2005) 385 (1): 207–216.
Published: 14 December 2004
...). Insulin increases the expression of the SREBP-1c gene in intact liver and in hepatocytes cultured in vitro . To learn the mechanism of this stimulation, we analysed the activation of the rat SREBP-1c promoter and its truncated or mutated congeners driving a luciferase reporter gene in transiently...
Articles
The gene encoding human retinoic acid-receptor-related orphan receptor α is a target for hypoxia-inducible factor 1
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Journal:
Biochemical Journal
Biochem J (2004) 384 (1): 79–85.
Published: 09 November 2004
.... In the present study we first demonstrate that hypoxia increases the amount of Rora transcripts in a wide panel of cell lines derived from diverse tissues. In addition, we identified a functional promoter sequence upstream of the first exon of the human Rora gene, spanning −487 and −45 from the translation...
Articles
Sp1 and Smad transcription factors co-operate to mediate TGF-β-dependent activation of amyloid-β precursor protein gene transcription
Available to PurchaseFabian DOCAGNE, Cecilia GABRIEL, Nathalie LEBEURRIER, Sylvain LESNÉ, Yannick HOMMET, Laurent PLAWINSKI, Eric T. MacKENZIE, Denis VIVIEN
Journal:
Biochemical Journal
Biochem J (2004) 383 (2): 393–399.
Published: 08 October 2004
... promoter, named APP TRE (APP TGF-β-responsive element); secondly, although this effect is mediated by a transduction pathway involving Smad3 (signalling mother against decapentaplegic peptide 3) and Smad4, Smad2 or other Smads failed to induce the activity of APP TRE . We also observed that the APP TRE...
Articles
Characterization and functional analysis of the 5′-flanking region of the mouse 20α-hydroxysteroid dehydrogenase gene
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Journal:
Biochemical Journal
Biochem J (2004) 382 (3): 975–980.
Published: 07 September 2004
... the oestrous cycles in these rodent species. We determined the nucleotide sequence of the 5′-flanking region of the mouse 20α-HSD gene, and examined its promoter activity using a rat luteinized granulosa cell culture. A reporter assay, using reporter constructs of various lengths of the 5′-flanking region...
Articles
Glutamine availability up-regulates expression of the amino acid transporter protein ASCT2 in HepG2 cells and stimulates the ASCT2 promoter
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Journal:
Biochemical Journal
Biochem J (2004) 382 (1): 27–32.
Published: 10 August 2004
... these conditions. The sequence of the 5′-flanking region of the ASCT2 gene was derived from the human genome database. A 907 bp fragment of this sequence was directionally ligated into a luciferase reporter vector and was shown to exhibit promoter activity when transfected into HepG2 cells. Promoter activity...
Articles
Epigenetic modifications affect Dnmt3L expression
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Journal:
Biochemical Journal
Biochem J (2004) 380 (3): 705–713.
Published: 15 June 2004
... family, is a regulator of maternal imprinting. In the present study, we have characterized the promoter region of the mouse Dnmt3L gene. Transient transfection assays performed with 5´-deletion promoter constructs indicated a minimal promoter area within 440 bp upstream from the translational start site...
Articles
Peroxisomal-proliferator-activated receptor alpha activates transcription of the rat hepatic malonyl-CoA decarboxylase gene: a key regulation of malonyl-CoA level
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Journal:
Biochemical Journal
Biochem J (2004) 378 (3): 983–990.
Published: 15 March 2004
...–4103]. This suggests that MCD may be transcriptionally regulated by PPARα. To investigate whether PPARα is truly responsible for transcriptional regulation of the rat MCD gene, transient reporter assay was performed in CV-1 cells. The promoter activity was increased by 17-fold in CV-1 cells co...
Articles
B-Myb acts as a repressor of human COL1A1 collagen gene expression by interacting with Sp1 and CBF factors in scleroderma fibroblasts
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Journal:
Biochemical Journal
Biochem J (2004) 378 (2): 609–616.
Published: 01 March 2004
... in SSc fibroblasts was correlated with decreased COL1A1 mRNA expression. Transient transfections localized the down-regulatory effect of B-Myb to a region containing the proximal 174 bp of the COL1A1 promoter that does not contain B-Myb consensus binding sites. Gel-shift analysis, using nuclear extracts...
Articles
Identification of a minimal promoter sequence for the human N-acetyltransferase Type I gene that binds AP-1 (activator protein 1) and YY-1 (Yin and Yang 1)
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Journal:
Biochemical Journal
Biochem J (2003) 376 (2): 441–448.
Published: 01 December 2003
..., although there are considerable differences in the level of activity between tissues. A search of the mRNA databases revealed the presence of several NAT1 transcripts in human tissue that appear to be derived from different promoters. Because little is known about NAT1 gene regulation, the present study...
Articles
Characterization of the human activator protein-2gamma (AP-2gamma) gene: control of expression by Sp1/Sp3 in breast tumour cells
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Journal:
Biochemical Journal
Biochem J (2003) 373 (3): 925–932.
Published: 01 August 2003
... at a single site within a classical initiator motif. We have gone on to investigate why some breast tumour-derived cell lines readily express AP-2γ, whereas others do not, and show that the proximal promoter (+191 to −312) is differentially active in the two cell phenotypes. DNase footprinting led...
Articles
Paraoxonase-1 promoter haplotypes and serum paraoxonase: a predominant role for polymorphic position −107, implicating the Sp1 transcription factor
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Journal:
Biochemical Journal
Biochem J (2003) 372 (2): 643–649.
Published: 01 June 2003
... the functions of both. There is a wide spectrum of serum PON1 mass in humans, to which promoter polymorphisms make an important contribution. The present studies attempted to define: (i) the relevance in vivo of promoter polymorphisms by analysing haplotype structure; and (ii) molecular mechanisms implicated...
Articles
Cyrus C. MARTIN, Christina A. SVITEK, James K. OESER, Eva HENDERSON, Roland STEIN, Richard M. O'BRIEN
Journal:
Biochemical Journal
Biochem J (2003) 371 (3): 675–686.
Published: 01 May 2003
... of the gluconeogenic pathway. The analysis of IGRP-chloramphenicol acetyltransferase (CAT) fusion-gene expression through transient transfection of islet-derived βTC-3 cells revealed that multiple promoter regions, located between −306 and −97, are required for maximal IGRP-CAT fusion-gene expression. These regions...
Articles
Myeloid zinc finger (MZF)-like, Kruppel-like and Ets families of transcription factors determine the cell-specific expression of mouse extracellular superoxide dismutase
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Journal:
Biochemical Journal
Biochem J (2003) 369 (2): 375–386.
Published: 15 January 2003
... mouse lung fibroblasts (MLg), kidney medulla (mIMCD3) and hepatocytes (Hepa 1-6). Real-time PCR showed that basal expression of EC-SOD was considerably higher in MLg cells compared with the other cell types. Reporter-gene assays revealed that the proximal promoter region was sufficient to support...
Articles
Valérie ANTONIO, Arthur BROUILLET, Brigitte JANVIER, Claire MONNE, Gilbert BEREZIAT, Marise ANDREANI, Michel RAYMONDJEAN
Journal:
Biochemical Journal
Biochem J (2002) 368 (2): 415–424.
Published: 01 December 2002
... is implicated in activation by synergistically acting cAMP and IL-1β. We transiently transfected and stimulated rat smooth muscle cells in primary culture and measured the promoter activities of serial and site-directed deletion mutants of sPLA 2 —luciferase constructs. A distal region, between −488 and −157 bp...
Articles
JFC1 is transcriptionally activated by nuclear factor-κB and up-regulated by tumour necrosis factor α in prostate carcinoma cells
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Journal:
Biochemical Journal
Biochem J (2002) 367 (3): 791–799.
Published: 01 November 2002
...Sergio D. CATZ; Bernard M. BABIOR; Jennifer L. JOHNSON The human promoter region of JFC1, a phosphatidylinositol 3,4,5-trisphosphate binding ATPase, was isolated by amplification of a 549bp region upstream of the jfc1 gene by the use of a double-PCR system. By primer extension analysis we mapped...
Articles
Signal transducers and activators of transcription 3 (STAT3) inhibits transcription of the inducible nitric oxide synthase gene by interacting with nuclear factor κB
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Journal:
Biochemical Journal
Biochem J (2002) 367 (1): 97–105.
Published: 01 October 2002
... assays with glutathione S-transferase-NF-κB p65 fusion protein and in vitro -translated STAT3α. Overexpression of STAT3 dramatically inhibited IL-1β- or LPS+IFN-γ-mediated induction of iNOS promoter-luciferase constructs that contained the wild-type iNOS promoter or ones harbouring mutated STAT-binding...
Articles
Multiple regions within the promoter of the murine Ifnar-2 gene confer basal and inducible expression
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Journal:
Biochemical Journal
Biochem J (2002) 365 (2): 355–367.
Published: 15 July 2002
... and II IFN showed that the muIfnar-2a and −2c mRNA isoforms were up-regulated independently of each other in L929 fibroblasts and hepa-1c1c7 hepatoma cells, but not in M1 myeloid leukaemia cells. Analysis of the 5′ flanking region of muIfnar-2 using promoter—luciferase reporter constructs defined three...
Articles
Identification of an initiator-like element essential for the expression of the tissue inhibitor of metalloproteinases-4 (Timp-4) gene
Available to PurchaseDavid A. YOUNG, Blaine W. PHILLIPS, Caroline LUNDY, Robert K. NUTTALL, Aileen HOGAN, Gilbert A. SCHULTZ, Kevin J. LECO, Ian M. CLARK, Dylan R. EDWARDS
Journal:
Biochemical Journal
Biochem J (2002) 364 (1): 89–99.
Published: 08 May 2002
... amplification of cDNA ends PCR identified multiple transcription starts for Timp-4 from brain and heart mRNA. The promoter region of Timp-4 was functional in transient transfection analysis in mouse C3H10T1/2 fibroblasts, where it directed basal expression that was non-inducible by serum. The TATA-less promoter...
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