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Keywords: proteasome
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Articles
Biochem J (2019) 476 (21): 3211–3226.
Published: 11 November 2019
... degraded primarily by the proteasome since several proteasome inhibitors blocked SK1 degradation, while lysosome, cathepsin B or pan caspase inhibitors had no effect. Importantly, we demonstrate that this proteasomal degradation of SK1 was enabled by its ubiquitination at Lys183 that appears facilitated by...
Articles
Biochem J (2017) 474 (9): 1547–1558.
Published: 24 April 2017
... higher affinity of G2385R compared with the wild-type protein for two proteins involved in proteasomal degradation, Hsc70 and carboxyl-terminus of Hsc70-interacting protein (CHIP). Overexpression of CHIP decreased intracellular protein levels of both G2385R mutant and wild-type LRRK2, while short...
Includes: Supplementary data
Articles
Biochem J (2017) 474 (4): 445–469.
Published: 03 February 2017
... proteins by targeting them for proteolysis via a process known as ER-associated degradation (ERAD). During ERAD, substrates are selected, modified with ubiquitin, removed from the ER, and then degraded by the cytoplasmic 26S proteasome. While integral membrane proteins can directly access the...
Articles
Biochem J (2016) 473 (17): 2655–2670.
Published: 30 August 2016
... detrimental. hA interacted with 20S core and 19S lid subunits of the β-cell proteasomal complex, as suggested by immunoprecipitation and confocal microscopy studies, which subsequently resulted in a decrease in the proteasome's proteolytic activity in these cells. In vitro binding and activity assays...
Includes: Supplementary data
Articles
Biochem J (2015) 472 (3): 353–365.
Published: 27 November 2015
...Alice Zuin; Anne Bichmann; Marta Isasa; Pilar Puig-Sàrries; Luís Miguel Díaz; Bernat Crosas Despite the progress made in understanding the roles of proteasome polyubiquitin receptors, such as the subunits Rpn10 (regulatory particle non-ATPase 10) and Rpn13, and the transient interactors Rad23...
Includes: Supplementary data
Articles
Biochem J (2015) 469 (3): 455–467.
Published: 23 July 2015
... process of monoubiquitination of the proteasomal subunit Rpn10 (regulatory particle non-ATPase 10), involved in the recruitment of polyubiquitinated substrates. Rpn10 is monoubiquitinated in vivo by the Nedd4 (neural precursor cell expressed developmentally down-regulated 4) enzyme Rsp5 (reverses SPT...
Includes: Supplementary data
Articles
Biochem J (2015) 466 (3): 571–585.
Published: 06 March 2015
...Jennifer L. Kopanic; Barbara Schlingmann; Michael Koval; Alan F. Lau; Paul L. Sorgen; Vivian F. Su The connexin43-interacting protein of 75 kDa directly interacts with the gap junction proteins connexin32, connexin40 and connexin45, facilitating their proteasomal degradation. In the present study...
Includes: Supplementary data
Articles
Biochem J (2014) 459 (3): 513–524.
Published: 11 April 2014
... phosphorylation, which targets the protein for proteasome-dependent destruction. In the present study, we examined ERK1/2-dependent regulation of BIK, drawing comparisons with BIM EL (BCL2-interacting mediator of cell death; extra long), a well-known target of ERK1/2. In many ERK1/2-dependent tumour cell lines...
Includes: Supplementary data
Articles
Biochem J (2014) 459 (2): 301–312.
Published: 28 March 2014
... (SLC10A1), OSTα (SLC51A) and ABCG1 (ATP-binding cassette G1), remained unaffected. ASBT inhibition by RSV was reversed by proteasome inhibitors (MG-132 and lactacystin) and the ubiquitin inhibitor LDN57444, suggesting involvement of the ubiquitin–proteasome pathway. Immunoprecipitation revealed high levels...
Articles
Biochem J (2014) 459 (1): 205–216.
Published: 14 March 2014
... process, p97 binds polyubiquitinated ERAD substrates and couples ATP hydrolysis to their dislocation from the ER as a prerequisite to destruction by the proteasome. The ubiquitin signals important for this process are not fully understood. In the present paper we report that p97 interacts with Lys 11...
Articles
Biochem J (2014) 458 (3): 459–467.
Published: 28 February 2014
...Joseph R. Tran; Jeffrey L. Brodsky The 26S proteasome is responsible for most regulated protein turnover and for the degradation of aberrant proteins in eukaryotes. The assembly of this ~2.5 MDa multicatalytic protease requires several dedicated chaperones and, once assembled, substrate selectivity...
Includes: Supplementary data
Articles
Biochem J (2014) 458 (1): 57–67.
Published: 20 January 2014
... (ubiquitin-like) domain-UBA (ubiquitin-associated) domain protein, CIP75 (connexin43-interacting protein of 75 kDa), regulates the proteasomal degradation of Cx43. Subcellular fractionation studies indicated that CIP75 interacts with Cx43 that is localized to the membrane of the ER (endoplasmic reticulum...
Articles
Biochem J (2014) 458 (1): 95–107.
Published: 20 January 2014
... ligand activators such as CITCO {6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O -(3,4-dichlorobenzyl)oxime} or through indirect chemical activation, such as with PB (phenobarbital). In the present study, we demonstrate that proteasomal inhibition markedly disrupts CAR function, repressing...
Includes: Supplementary data
Articles
Biochem J (2014) 457 (1): 99–105.
Published: 10 December 2013
... fails to exit the ER. Mutant PCSK9s which failed to undergo autocatalytic cleavage or failed to exit the ER, failed to increase the amount of WT-ED-LDLR in the medium. These mutants also reduced the amount of WT-ED-LDLR intracellularly, which could partly be prevented by the proteasome inhibitor...
Includes: Supplementary data
Articles
Biochem J (2013) 453 (3): 435–445.
Published: 12 July 2013
...) where its catalytic A chain (RTA) is reductively separated from the holotoxin to enter the cytosol and inactivate ribosomes. The currently accepted model is that the bulk of ER-dislocated RTA is degraded by proteasomes. We show in the present study that the proteasome has a more complex role in ricin...
Includes: Supplementary data
Articles
Biochem J (2013) 452 (3): 391–400.
Published: 31 May 2013
...-terminal and the membrane domain. Together, these studies support the idea that certain misprocessing mutations alter intramolecular interactions within the full-length ClC-5 protein. Further, we found that these misfolded mutants are polyubiquitinated and targeted for proteasomal degradation in the OK...
Articles
Biochem J (2013) 451 (3): 427–437.
Published: 12 April 2013
... (hypoxia-inducible factor 1α) from proteasomal degradation, suggesting that it inhibits the proteasome. The results of the present study indicate that the anti-inflammatory effects of BAY 11-7082, its ability to induce B-cell lymphoma and leukaemic T-cell death and to prevent the recruitment of proteins to...
Includes: Supplementary data
Articles
Biochem J (2012) 448 (1): 55–65.
Published: 18 October 2012
...Jonas Boehringer; Christiane Riedinger; Konstantinos Paraskevopoulos; Eachan O. D. Johnson; Edward D. Lowe; Christina Khoudian; Dominique Smith; Martin E. M. Noble; Colin Gordon; Jane A. Endicott The ubiquitin–proteasome system targets selected proteins for degradation by the 26S proteasome. Rpn12...
Includes: Supplementary data
Articles
Biochem J (2012) 445 (3): 377–382.
Published: 13 July 2012
... unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. cytochrome P450 immunoproteasome interleukin-1 (IL-1) nitric oxide (NO) proteasome Since its discovery as the endothelium-derived factor that relaxes vascular smooth...
Includes: Supplementary data
Articles
Biochem J (2012) 441 (3): 927–939.
Published: 16 January 2012
... (ubiquitylation) targets many substrates, often leading to their proteasomal degradation. NEDD8 (neural-precursor-cell-expressed developmentally down-regulated 8) is the UBL most closely related to ubiquitin, and its best-studied role is the activation of CRLs (cullin-RING ubiquitin ligases) by its conjugation to...
Includes: Supplementary data
Articles
Biochem J (2011) 436 (3): 559–566.
Published: 27 May 2011
.... Consistent with these results, evidence is presented that processing by the proteasome is required for TDP1 cleavage in vivo . 1 Present address: Institute of Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, U.K. 2 To whom correspondence should be addressed (email champoux...
Articles
Biochem J (2010) 432 (1): 217–226.
Published: 25 October 2010
...Sandra P. Melo; Asami Yoshida; Franklin G. Berger Human thymidylate synthase (hTS; EC 2.1.1.45) is one of a small group of proteasomal substrates whose intracellular degradation occurs in a ubiquitin-independent manner. Previous studies have shown that proteolytic breakdown of the hTS polypeptide...
Articles
Biochem J (2010) 431 (3): 391–402.
Published: 11 October 2010
... activation. 1 To whom correspondence should be addressed (email micln@nus.edu.sg ). 28 7 2010 23 8 2010 26 8 2010 26 8 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 de-ubiquitination proteasome protein degradation regulation of gene...
Includes: Supplementary data
Articles
Biochem J (2010) 429 (1): 127–136.
Published: 14 June 2010
... previously been shown to be modulated by RNF12 (RING finger protein 12). RNF12 is an E3 ubiquitin ligase that can target LDB1 for poly-ubiquitination and degradation via the proteasome. We find that in HEK (human embryonic kidney)-293 cells expression of RNF12 leads to mono-ubiquitination of LDB1 and...
Articles
Biochem J (2010) 427 (3): 435–443.
Published: 14 April 2010
..., blockade of the proteasome through epoxomicin or MG132 abolishes PMA-induced production of the CTF of MLK3. This CTF is able to heterodimerize with full-length MLK3, and interact with the active form of the small GTPase Cdc42, resulting in diminished activation loop phosphorylation of MLK3 and reduced...
Includes: Supplementary data
Articles
Biochem J (2010) 426 (1): 13–17.
Published: 27 January 2010
...Vian Azzu; Shona A. Mookerjee; Martin D. Brand UCP3 (uncoupling protein 3) and its homologues UCP2 and UCP1 are regulators of mitochondrial function. UCP2 is known to have a short half-life of approx. 1 h, owing to its rapid degradation by the cytosolic 26S proteasome, whereas UCP1 is turned over...
Articles
Biochem J (2010) 425 (1): 225–236.
Published: 14 December 2009
... inhibitor Z-IETD-FMK (benzyloxycarbonyl-Ile-Glu-Thr-Asp-fluoromethylketone). ALP-resistant cells showed decreased Fas expression, at both the mRNA and protein levels, in a proteasome-dependent fashion. The proteasome inhibitor MG132 partially restored Fas expression and resensitized the cells to FasL, but...
Includes: Supplementary data
Articles
Biochem J (2009) 421 (3): 397–404.
Published: 15 July 2009
...Xiaohua Li; George N. Demartino The 26S proteasome is a 2500 kDa protease complex that degrades polyubiquitylated proteins by a mechanism that requires ATP hydrolysis. It also degrades short non-ubiquitylated peptides and certain unstructured proteins by an energy-independent mechanism that...
Articles
Biochem J (2009) 417 (1): 149–165.
Published: 12 December 2008
... (nuclear factor κB) activation. Additionally, through their E3 ubiquitin ligase activities, c-IAP1 and c-IAP2 promote proteasomal degradation of NIK (NF-κB-inducing kinase) and regulate the non-canonical NF-κB pathway. In the present paper, we describe a novel ubiquitin-binding domain of IAPs. The UBA...
Includes: Supplementary data
Articles
Biochem J (2008) 414 (2): 161–175.
Published: 12 August 2008
... by the proteasome, thereby resulting in the protection of prostate cancer cells from apoptosis. The finding that USP2a is involved in human prostate cancer, coupled with information derived from the crystal structure of the USP2a catalytic domain [ 34 ], has made it a potential target for therapeutic...
Articles
Biochem J (2008) 411 (3): 623–631.
Published: 14 April 2008
... at markedly low levels, whereas their mRNA levels were equal to those of the other SNP variants and ABCG2 WT (wild-type). Interestingly, protein expression levels of the ABCG2 F208S and S441N variants increased 6- to 12-fold when Flp-In-293 cells were treated with MG132, a proteasome inhibitor...
Articles
Biochem J (2008) 411 (3): 581–591.
Published: 14 April 2008
... complex is well conserved in Saccharomyces cerevisiae and has been characterized as a FBPase (fructose-1, 6-bisphosphatase)-degrading complex. The yeast homologue of ARMc8, Gid (glucose-induced degradation) 5p, plays an essential role in the ubiquitin- and proteasome-dependent degradation of FBPase. To...
Articles
Biochem J (2008) 411 (2): 387–397.
Published: 27 March 2008
...Hala Chamieh; Dorian Guetta; Bruno Franzetti The PAN (proteasome-activating nucleotidase) proteins from archaea represent homologues of the eukaryotic 26S proteasome regulatory ATPases. In vitro the PAN complex has been previously shown to have a stimulatory effect on the peptidase activities of...
Articles
Biochem J (2008) 410 (2): 401–407.
Published: 12 February 2008
...Junko Takeuchi; Hui Chen; Martin A. Hoyt; Philip Coffino Mouse ODC (ornithine decarboxylase) is quickly degraded by the 26S proteasome in mammalian and fungal cells. Its degradation is independent of ubiquitin but requires a degradation signal composed of residues 425–461 at the ODC C-terminus...
Articles
Biochem J (2008) 410 (1): 131–140.
Published: 29 January 2008
... Society 2008 aging cathepsin B dopa lysosome oxidized protein proteasome Oxidized protein deposition and accumulation have been implicated in the aetiology of a wide variety of age-related pathologies, including neurodegenerative diseases (for a review, see [ 1 ]), atherosclerosis [ 2...
Articles
Biochem J (2008) 409 (1): 205–213.
Published: 11 December 2007
... protein-degradation machinery of the cell. RNAi-targeted removal of most proteasome subunits in C. elegans caused nuclear localization of SKN-1 and, in some cases, induced transcription of gst - 4 . Most intriguingly, RNAi knockdown of proteasome core subunits caused nuclear localization of SKN-1 and...
Articles
Biochem J (2007) 408 (2): 259–266.
Published: 14 November 2007
... different cell lines or in differentiated brown adipocytes. Proteasome inhibitors specifically increased the stability of both transfected and endogenous Cidea protein. Furthermore, Cidea protein was found to be polyubiquitinated when overexpressed in different culture cells as well as in differentiated...
Articles
Biochem J (2007) 405 (2): 341–349.
Published: 27 June 2007
... growth-arrested mammary epithelial cells but the C/EBPδ protein exhibits a t 1/2 of only ∼120 min. The goal of the present study was to investigate the role of C/EBPδ modification by ubiquitin and C/EBPδ proteasome-mediated degradation. Structural and mutational analyses demonstrate that an intact...
Articles
Biochem J (2007) 404 (3): 353–363.
Published: 29 May 2007
... endoplasmic-reticulum-associated degradation (ERAD) folding heat-shock protein 70 (Hsp70) molecular chaperone proteasome ubiquitin Protein biogenesis is an inherently error-prone process, given the high rate of translation, the synthesis of polypeptides that may be encoded by mutated genes and...
Articles
Biochem J (2007) 404 (3): 487–497.
Published: 29 May 2007
..., and was dependent on the proteasome. The susceptibility of Pak1 to Chp-induced degradation depended on its p21-binding domain, kinase activity and a number of Pak1 autophosphorylation sites, whereas the PIX- (Pak-interacting exchange factor) and Nck-binding sites were not required. Together, these...
Articles
Biochem J (2006) 400 (3): 511–520.
Published: 28 November 2006
... show that tau turnover is dependent on degradation by the proteasome (inhibited by MG132) in HT22 neuronal cells. Recombinant human tau was rapidly degraded by the 20 S proteasome in vitro , but tau phosphorylation by GSK3β (glycogen synthase kinase 3β) significantly inhibited proteolysis. Tau...
Articles
Biochem J (2006) 399 (3): 361–372.
Published: 13 October 2006
... Biochemical Society, London 2006 endocytosis proteasome protein structure ubiquitination ubiquitin-binding domain vesicle trafficking Ubiquitin is a 76-amino-acid protein ( Figure 1 ), so-named for its extraordinarily wide distribution from yeast to man [ 1 ]. The covalent ubiquitination of...
Articles
Biochem J (2006) 394 (2): 501–509.
Published: 10 February 2006
... show that, in both HEK-293 cells and the TSM1 neuronal cell line, the immunoreactivities of overexpressed myc-tagged-APH1a and -PEN2 were enhanced by the proteasome inhibitors ZIE and lactacystin, whereas a broad range of protease inhibitors had no effect. By contrast, proteasome inhibitors were...
Articles
Biochem J (2006) 394 (1): 267–273.
Published: 27 January 2006
.... In the present study, we have evaluated the contribution of the ubiquitin–26 S proteasome pathway to the diet-induced changes in CDO half-life. In the living rat, inhibition of the proteasome with PS1 (proteasome inhibitor 1) dramatically stabilized CDO in the liver under dietary conditions that...
Articles
Biochem J (2006) 394 (1): 355–363.
Published: 27 January 2006
... 26 S proteasome in a ubiquitin-independent manner. Such degradation is directed by the disordered N-terminal region of the TS polypeptide, and is abrogated by ligand binding. In the present study, we have verified the ubiquitin-independent nature of TS proteolysis by showing that a ‘lysine-less...
Articles
Biochem J (2006) 393 (1): 303–309.
Published: 12 December 2005
... and fission yeast that do not have BRCA2-related proteins, indicating that DSS1 has a physiological role independent of BRCA2. The DSS1 orthologue in Saccharomyces cerevisiae has been shown to associate with the 26 S proteasome and, in the present paper, we report that in the distantly related fission...
Articles
Biochem J (2005) 392 (2): 271–281.
Published: 22 November 2005
...Daniel Bailey; Peter O'Hare To investigate potential interplay between the SUMO1 (small ubiquitin-related modifier-1) and ubiquitin pathways of post-translational protein modification, we examined aspects of their localization and conjugation status during proteasome inhibition. Our results...
Articles
Biochem J (2005) 391 (3): 513–525.
Published: 25 October 2005
... the proteasome for degradation. However, through proteasome inhibition studies, we show that this does not occur. Instead, our results suggest that ubiquilin regulates PS fragment production. We also examined whether other components of the γ-secretase complex are affected by ubiquilin expression...
Articles
Biochem J (2005) 388 (2): 647–655.
Published: 24 May 2005
... ER-associated protein degradation. TEB4 is an ER degradation substrate itself, promoting its own degradation in a RING finger- and proteasome-dependent manner. 1 To whom correspondence should be addressed (email Wiertz@lumc.nl ). 22 7 2004 23 12 2004 27 1 2005 27 1 2005...
Articles
Biochem J (2005) 387 (3): 897–903.
Published: 26 April 2005
...Gerda DONOSO; Volker HERZOG; Anton SCHMITZ Misfolded proteins are removed from the ER (endoplasmic reticulum) by retrotranslocation to the cytosol and degradation by the ubiquitin–proteasome system in a process designated ERAD (ER-associated degradation). Analysing the turnover of a misfolded form...