1-50 of 107
Keywords: protein–protein interaction
Close
Sort by
Articles
Biochem J (2024) 481 (1): 17–32.
Published: 04 January 2024
... protein calmodulin phosphorylation proteinprotein interaction water permeability Light reaches the retina of the eye, allowing us to perceive the world. Before it does, it passes through the lens, which focuses the light, and makes the image sharp. A lens has many unique properties, which...
Includes: Supplementary data
Articles
Biochem J (2023) 480 (6): 421–432.
Published: 24 March 2023
... Chaetocin may also non-specifically inhibit proteinprotein interactions. As we previously showed that cryptochrome circadian regulator (CRY2) and calcium- and integrin-binding protein 1 (CIB1) interact upon blue light stimulation in a similar two-hybrid assay [ 11 ], we can use this system as a convenient...
Includes: Supplementary data
Articles
Articles
Articles
Biochem J (2018) 475 (16): 2667–2679.
Published: 31 August 2018
... bands were quantified by densitometry and analyzed using Multi Gauge software (Fujifilm Co., Ltd). © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 crystal structure NuRD proteinprotein interaction RBBP4 zinc finger ZNF827...
Articles
Biochem J (2017) 474 (22): 3747–3761.
Published: 01 November 2017
... to be a highly versatile domain mediating proteinprotein interactions in a large number of transcriptional repressors as well as E3 ubiquitin ligases [ 3 ]. BTB domain interaction with Cul3 is required for certain KCTD family members to assemble into CRL3s. To investigate the potential...
Includes: Supplementary data
Articles
Articles
Biochem J (2017) 474 (7): 1109–1125.
Published: 15 March 2017
...Patrick G. Dougherty; Ziqing Qian; Dehua Pei Macrocyclic compounds such as cyclic peptides have emerged as a new and exciting class of drug candidates for inhibition of intracellular proteinprotein interactions, which are challenging targets for conventional drug modalities (i.e. small molecules...
Articles
Biochem J (2016) 473 (21): 3889–3901.
Published: 27 October 2016
...); published by Portland Press Limited on behalf of the Biochemical Society 2016 core domain DNAJA1/HSP40 in situ cross-linking activity proteinprotein interaction transamidation transglutaminase 2 Transglutaminase 2 (TG2) is a widely expressed multifaceted enzyme with distinct...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (16): 2463–2469.
Published: 11 August 2016
...Jiwon Hwang; Peter J. Espenshade The engineered ascorbate peroxidase (APEX2) has been effectively employed in mammalian cells to identify proteinprotein interactions. APEX2 fused to a protein of interest covalently tags nearby proteins with biotin-phenol (BP) when H 2 O 2 is added to the cell...
Includes: Supplementary data
Articles
Articles
Articles
Articles
Biochem J (2015) 465 (2): 325–335.
Published: 06 January 2015
... specificity proteinprotein interaction Streptococcus pneumoniae structural biology The complement system is a major component of vertebrate innate immunity and one of the most important frontline defenders against microbial invaders [ 1 , 2 ]. Uncontrolled or inappropriate complement activation...
Includes: Supplementary data
Articles
Biochem J (2015) 465 (2): 247–257.
Published: 06 January 2015
... Leishmania peroxin PEX14 PEX5 protein translocation proteinprotein interaction The human protozoan parasite Leishmania is the causative agent of a spectrum of diseases known as leishmaniasis, a disease that currently affects ~12 million people in 88 countries in tropical and subtropical...
Articles
Biochem J (2014) 462 (3): 555–565.
Published: 22 August 2014
... to the endoplasmic reticulum membrane. coiled-coil domain multiprotein complex proteinprotein interaction selenocysteine selenoprotein S HEK-293T cells (5×10 8 ) transfected with HHT-SelS CU, HHT-SelS CC, HHT-SelS C, HHT-SelS-∆ CC and HT-SelS- CT constructs were collected in PBS buffer 48–61...
Articles
Biochem J (2014) 462 (2): 337–345.
Published: 07 August 2014
... protein described above, since the low activity detected in the presence of AA-activated p67 phox with gp91 phox derived from its low capacity to develop proteinprotein interactions with gp91 phox . In the absence of p22 phox , the active complex interactions might have been modified in which...
Articles
Articles
Biochem J (2014) 459 (1): 15–25.
Published: 14 March 2014
... phosphorylation. 14-3-3 protein Arabidopsis calcium-dependent protein kinase kinase assay proteinprotein interaction tyrosine phosphorylation To determine whether CPKs can phosphorylate 14-3-3 isoforms χ and ε, a series of kinase assays were performed using phylogenetically distinct CPKs (1, 3...
Includes: Supplementary data
Articles
Biochem J (2014) 457 (1): 137–149.
Published: 10 December 2013
... CUB domains inhibit angiogenesis in vitro . affinity extracellular matrix kinetics network proteinprotein interaction surface plasmon resonance PCPE-1 (procollagen C-proteinase enhancer-1) and PCPE-2 are two closely related ECM (extracellular matrix) glycoproteins that, although...
Includes: Supplementary data
Articles
Biochem J (2013) 454 (3): 459–466.
Published: 29 August 2013
... optineurin proteinprotein interaction selective autophagy X-ray crystallography Additionally we have solved the NMR structure of LC3B in complex with the phosphorylated LIR motif of OPTN and the crystal structures of LC3B free and in complex with a phospho-mimicking LIR mutant...
Includes: Supplementary data
Articles
Biochem J (2013) 453 (3): 475–485.
Published: 12 July 2013
... oxidative stress proteinprotein interaction signalling thiol The pET28a-His-Prx2 construct, which has been described previously [ 28 ], encodes N-terminally His-tagged human Prx2 with a linker sequence of 30 amino acids. Mutagenesis reactions to create peroxidative (C51S) or resolving (C172S...
Includes: Supplementary data
Articles
Biochem J (2013) 453 (2): 219–230.
Published: 28 June 2013
... (LRET) microsome oligomerization proteinprotein interaction α-naphthoflavone Analysis of series of spectra obtained in fluorescence spectroscopy experiments was done by PCA (principal component analysis) [ 35 , 36 ]. To resolve the changes in the fluorescence of the donor and acceptor...
Articles
Articles
Biochem J (2013) 450 (3): 497–509.
Published: 28 February 2013
... 2012 © The Authors Journal compilation © 2013 Biochemical Society 2013 California poppy ( Eschscholzia californica ) cyclophilin phospholipase A 2 plant G-proteins plant plasma membrane plant signalling proteinprotein interaction Heterotrimeric G-proteins are conserved...
Includes: Supplementary data
Articles
Articles
Articles
Biochem J (2013) 449 (3): 649–659.
Published: 09 January 2013
... 23 10 2012 23 10 2012 © The Authors Journal compilation © 2013 Biochemical Society 2013 apoptosis-stimulating protein of p53 (ASPP) protein phosphatase-1 catalytic subunit (PP-1c) proteinprotein interaction RVXF-binding motif Src homology 3 domain (SH3 domain) Nearly...
Includes: Supplementary data
Articles
Biochem J (2013) 449 (2): 415–425.
Published: 14 December 2012
... images were collected at an oscillation angle of 1°. Raw data images were processed using HKL2000 [ 19 ]. binding-induced folding lipoate protein ligase lipoyl domain NMR spectroscopy proteinprotein interaction X-ray crystallography Aerobic metabolism of 2-oxoacids and C1 metabolism...
Articles
Biochem J (2012) 448 (3): 373–387.
Published: 21 November 2012
... causing major alterations to amylopectin architecture. The present study reports a biochemical and molecular analysis of an allelic variant of the sugary-2 mutation expressing a catalytically inactive form of SSIIa and sheds new light on its central role in proteinprotein interactions and determination...
Includes: Supplementary data
Articles
Biochem J (2012) 444 (2): 261–267.
Published: 11 May 2012
... binding. Replacement of Cys 65 in the ethylene-binding domain by serine dramatically reduced binding of radiolabelled cyanide. In contrast with wild-type ETR1, autokinase activity of the receptor is not reduced in the ETR1-C65S mutant upon addition of cyanide. Additionally, proteinprotein interaction...
Includes: Supplementary data
Articles
Biochem J (2012) 442 (3): 573–581.
Published: 24 February 2012
... of the interaction domain in DREB2A appears to be required for proper DREB2A function under stress conditions. abiotic stress alternative splicing CMIV-3 domain proteinprotein interaction protein stability transcription factor Protein structure predictions for the RST domain of RCD1 and for the C...
Includes: Supplementary data
Articles
Articles
Articles
Biochem J (2011) 439 (2): 313–319.
Published: 28 September 2011
... 6 2011 28 6 2011 28 6 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 cardiac Ca 2+ -ATPase enzyme regulation equilibrium phospholamban proteinprotein interaction The cardiac muscle Ca 2+ -ATPase [SERCA2a (sarcoplasmic/endoplasmic reticulum...
Articles
Articles
Articles
Biochem J (2011) 438 (1): 191–202.
Published: 27 July 2011
... β-adrenoceptor β-arrestin-2 G-protein-coupled receptor (GPCR) ligand efficacy proteinprotein interaction resonance energy transfer Although β 1 - and β 2 -AR (adrenoceptor) subtypes are structurally alike [ 1 , 2 ] and interact similarly with the G-protein G s , which controls...
Includes: Supplementary data
Articles
Articles
Biochem J (2011) 436 (2): 429–436.
Published: 13 May 2011
... The Authors Journal compilation © 2011 Biochemical Society 2011 drug target fusicoccin glycoprotein Ib-IX-V platelet aggregation 14-3-3 protein proteinprotein interaction receptor toxin FC (fusicoccin) ( Figure 1 A), a diterpene glucoside produced by fungus Phomopsis amygdali...
Articles
Articles
Biochem J (2011) 435 (3): 701–709.
Published: 13 April 2011
... adenosine deaminase adenosine receptor allosteric interaction G-protein-coupled receptor proteinprotein interaction receptor binding parameter Self-association of proteins to form dimers and higher-order oligomers and/or interaction with other proteins are key factors in cell signalling [ 1...
Articles
Biochem J (2011) 435 (3): 711–722.
Published: 13 April 2011
... 2011 14 2 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 calcium calmodulin connexin50 gap junction junctional conductance proteinprotein interaction The circulation of current fluid flow through the closely packed cells of the lens of the eye...
Includes: Supplementary data
Articles
Biochem J (2011) 435 (2): e1–e4.
Published: 29 March 2011
... the idea that the cAMP-dependent channel activity of CFTR could be effectively enhanced by disruption of a proteinprotein interaction which is normally inhibitory for the production of cAMP. This particular proteinprotein interaction [between the PDZ motif of LPA 2 (type 2 lysophosphatidic acid receptor...
Articles
Articles
Biochem J (2010) 431 (1): 23–29.
Published: 14 September 2010
... E3 ligase protein domain proteinprotein interaction really interesting new gene finger (RING finger) RING finger protein 4 (RNF4) small ubiquitin-like modifier (SUMO) ubiquitin RING (really interesting new gene) domain ubiquitin ligases promote the transfer of ubiquitin from...
Includes: Supplementary data
Articles
Articles
Biochem J (2010) 430 (3): e5–e6.
Published: 27 August 2010
... of complex proteins)] domain. As well as the two catalytic domains, LRRK2 possesses several proteinprotein interaction domains, but their function and the proteins with which they interact are poorly understood. In this issue of the Biochemical Journal , Nichols et al. study the interaction of the N...
Articles
Articles
Biochem J (2010) 427 (3): 467–475.
Published: 14 April 2010
... [email protected] ). 15 10 2009 5 2 2010 15 2 2010 15 2 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 endostatin (ES) extracellular matrix proteinprotein interaction surface plasmon resonance (SPR) binding assays transglutaminase-2 (TG-2...
Includes: Supplementary data