... and the Biochemical Society under a transformative agreement with CAUL. lipid kinase membrane proteins phosphoinositide 3-kinase PIK3CA protein conformation small molecules Class I PI3Ks catalyse the conversion of membrane embedded phosphatidylinositol(4,5)-bisphosphate (PIP 2...

... and the substrate. Thus, a regulated docking interaction between a substrate and its upstream kinase enables the differential phosphorylation of specific substrates by a protein kinase. AGC kinase allosteric regulation PDPK1 phosphorylation/dephosphorylation protein conformation protein kinase...

.... The media was then changed after 6 h. Experiments were conducted 24 h after transfection. cellular targeting endoplasmic reticulum ERAD molecular chaperones protein conformation ubiquitin proteasome system The regulation of salt and fluid balance across membranes is an essential function...

... the structure–function model stating that the unique 3D structure is a prerequisite of specific protein function was a dominating paradigm in the scientific minds. intrinsically disordered proteins protein conformation protein structure structure–function continuum © 2021 The Author(s...

... Commons Attribution License 4.0 (CC BY) . ARHL circular cross-correlation interaction networks protein conformation statistical and computational modeling tip-link proteins Table 1. WSME Model Parameters WT S47P PDB ID 4AQ8 4AQE ξ (van der Waals interaction...

... aggregation HSP malaria plasmodium protein conformation The human malaria parasite encounters frequent environmental fluctuations (pH, nutrients, temperature, oxygen) and immune threats as it homes different tissues; and transits through warm-blooded host (human for asexual cycle) and cold-blooded...

... be very challenging to extricate, it should still be possible to relate the sequence patterns to the energetic outcomes and the eventual impact on folding and function. kinetic stability point mutations protein conformation sequence evolution statistical model thermodynamics...

..., U.S.A.) for band density analysis. Both techniques were performed according to the manufacturer's instructions. Band densities were calculated using GelAnalyzer 9.1 ( http://www.gelanalyzer.com/ ). α-lactalubumin β-casein mass spectrometry molecular chaperones protein aggregation protein...

... with the experimental SAXS data using CRYSOL [ 32 ]. crystallography GTPases hydrogen–deuterium exchange mass spectrometry leucine-rich repeat kinase protein conformation Roco proteins SEC-MALS (multi-angle light scattering) analysis was performed by injecting 10 µl of an 8 mg ml −1 protein sample...

... ) 3 4 2018 16 8 2018 28 8 2018 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 cytoskeleton molecular chaperones protein conformation Chaperonin proteins are ATPases which assemble into single- and double-ring...

... binding IDP molecular interactions nuclear–protein protein conformation protein stability NUPR1 is an 82-residue-long (8 kDa), highly basic, monomeric IDP that is overexpressed during the acute phase of pancreatitis [ 1 , 2 ]. It does not have a stable secondary and tertiary structure in any...

... 27 2 2018 1 3 2018 2 3 2018 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 apoptosis CARD caspase-9 circular dichroism interdomain interactions protein conformation Apoptosis or programmed cell death...

... Å). protein conformation protein structure RNA polymerase RpoS Salmonella sigma factor The ability to adapt quickly to variations of environmental conditions is crucial for the growth and survival of bacteria in their natural environments. One major strategy used by bacteria, to co-ordinate...

... with 1 M H 2 SO 4 , and an endpoint measurement of HRP activity at 450 nm was made using a SpectraMax M2e plate reader (Molecular Devices). All steps were performed at room temperature. allosteric regulation antibodies protein aggregation protein conformation protein–protein interactions...

... and increases solubility. Alzheimer's disease amyloid BRICHOS molecular chaperone protein conformation protein inclusions In neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease, amyloid formation is believed to be a main cause [ 1 – 3 ]. Although amyloid-forming...

... 119 phosphorylation modulates PRRXL1 conformation and transcriptional activity. embryonic and fetal development nociception paired related homeobox-like 1 (PRRXL1) phosphorylation protein conformation spinal cord dSC nuclear lysates for 1D PAGE were obtained as described...

... phosphorylation of the NF-κB subunit p65 results in conformational changes of the protein. heat-shock protein p65 protein conformation nuclear factor κB (NF-κB) transcription The NF-κB (nuclear factor κB) DNA-binding subunits p65 (RelA), p50 (NFKB1), p52 (NFKB2), c-Rel and RelB form homo...

... disease prion protein (PrP) protein conformation transgenic Mammalian prion diseases are fatal neurodegenerative conditions of humans and various other vertebrate species. These diseases include scrapie in sheep, BSE (bovine spongiform encephalopathy) in cattle and CJD (Creutzfeldt–Jakob...

... [email protected] ). 3 2 2010 17 3 2010 29 3 2010 29 3 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 disulfide bond pattern metalloproteinase protein conformation protein disulfide isomerase (PDI) redox regulation shedding...

... of the prion diseases, a detailed mechanistic understanding of this conversion process is yet to be elucidated [ 4 , 16 ]. disulfide bond prion prion protein protein conformation Human prion protein contains a single disulfide bond linking Cys 179 and Cys 214 [ 17 , 18 ]. The role...

...) glucokinase disease (GK disease) glucokinase protein conformation tryptophan fluorescence A leading player in the regulation of blood glucose is the enzyme GK (glucokinase; ATP: D -glucose-6-phosphotransferase; EC 2.7.1.1). GK has low affinity for glucose, is not under feedback control by hexose...

..., a panel of four monoclonal antibodies was raised to the denatured protein and used as probes of protein conformation. The linear sequence epitope reactivity of a polyclonal antibody raised against the native protein and the monoclonal antibodies were defined and mapped to distinct regions on the IDS...

... translocase, atractyloside (ATR), mito- chondrial transport protein, protein conformation. INTRODUCTION The mitochondrial ADP/ATP translocase (AAT) catalyses the exchange, across the inner membrane of the organelle, of cytosolic ADP for ATP, produced in the matrix by ATP-synthase. The AAT is the most highly...

... The Biochemical Society, London © 2000 2000 actin binding phosphorylation protein conformation protein kinase C smooth muscle tropomyosin Biochem. J. (2000) 350, 579 588 (Printed in Great Britain) 579 A role for serine-175 in modulating the molecular conformation of calponin Jian-Ping JIN*1, Michael...

... (C91T). The C91T mutation caused a loss of 4-sulphatase activity, a detectable protein conformational change and a lower level of intracellular 4-sulphatase protein [Brooks, Robertson, Bindloss, Litjens, Anson, Peters, Morris and Hopwood (1995) Biochem. J. 307 , 457-463]. In the present study, we report...

... Society, London © 1999 1999 metal binding NMR spectroscopy protein conformation stability constant serum protein Biochem. J. (1999) 337, 105 111 (Printed in Great Britain) 105 N-lobe versus C-lobe complexation of bismuth by human transferrin Hongzhe SUN*, Hongyan LI*, Anne B. MASON‹, Robert C...