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Articles

Development of an OTUD1 ubiquitin variant inhibitor

Qi Liu, Evan Mallette, Hui Zheng, Wei Zhang

Biochem J (2023) 480 (16): 1317–1330.

https://doi.org/10.1042/BCJ20230119

Published: 25 August 2023

... and deubiquitinase activity with the OTUD1 substrate RIPK1 were inhibited. Herein we describe the development of molecular tools for exploring the activity of OTUD1 in a cellular context, towards protein-based therapeutics. deubiquitinases inhibitor phage display protein engineering ubiquitin variants UIM...

View article titled, Development of an OTUD1 ubiquitin variant inhibitor

Includes: Supplementary data

Articles

Computational design of Matrix Metalloproteinase-9 (MMP-9) resistant to auto-cleavage

Alessandro Bonadio, Solomon Oguche, Tali Lavy, Oded Kleifeld, Julia Shifman

Biochem J (2023) 480 (14): 1097–1107.

https://doi.org/10.1042/BCJ20230139

Published: 17 July 2023

... in an all-inclusive Read & Publish agreement with Portland Press and the Biochemical Society under a transformative agreement with MALMAD. auto-cleavage auto-degradation enzyme engineering matrix metalloproteases MMP-9 protein engineering Matrix metalloproteinases (MMPs...

View article titled, Computational design of Matrix Metalloproteinase-9 (MMP-9) resistant to auto-cleavage

Includes: Supplementary data

Articles

Structural features of sensory two component systems: a synthetic biology perspective

Marcos Nieves, Alejandro Buschiazzo, Felipe Trajtenberg

Biochem J (2023) 480 (2): 127–140.

https://doi.org/10.1042/BCJ20210798

Published: 23 January 2023

... 2023 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2023 protein engineering signaling pathways synthetic biology All these data concerning the molecular means by which information is allosterically transmitted from the sensory end...

View article titled, Structural features of sensory two component systems: a synthetic biology perspective

Articles

Engineering potassium activation into biosynthetic thiolase

Andrew C. Marshall, John B. Bruning

Biochem J (2021) 478 (15): 3047–3062.

https://doi.org/10.1042/BCJ20210455

Published: 13 August 2021

... enzyme activation metalloenzymes molecular mechanisms protein engineering Metals are present as structural features in over one-third of proteins, underscoring their importance for protein structure and function [ 1 , 2 ]. The incorporation of divalent metal ions into protein structures...

View article titled, Engineering potassium activation into biosynthetic thiolase

Includes: Supplementary data

Articles

Quantitative mapping of binding specificity landscapes for homologous targets by using a high-throughput method

Lidan Aharon, Shay-Lee Aharoni, Evette S. Radisky, Niv Papo

Biochem J (2020) 477 (9): 1701–1719.

https://doi.org/10.1042/BCJ20200188

Published: 11 May 2020

... Papo ( [email protected] ) 3 3 2020 11 4 2020 14 4 2020 15 4 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 metalloproteases next-generation sequencing protease inhibitor protein engineering protein–protein...

View article titled, Quantitative mapping of binding specificity landscapes for homologous targets by using a high-throughput method

Includes: Supplementary data

Articles

Nek7 conformational flexibility and inhibitor binding probed through protein engineering of the R-spine

Matthew J. Byrne, Nazia Nasir, Christine Basmadjian, Chitra Bhatia, Rory F. Cunnison, Katherine H. Carr, Corine Mas-Droux, Sharon Yeoh, Céline Cano, Richard Bayliss

Biochem J (2020) 477 (8): 1525–1539.

https://doi.org/10.1042/BCJ20200128

Published: 29 April 2020

.... We also determined the structure of Nek2 bound to the same inhibitor, to enable a direct comparison of binding modes. These structures provide insights into the differences between Nek family members that might aid in the development of chemical inhibitors. kinases protein engineering small...

View article titled, Nek7 conformational flexibility and inhibitor binding probed through protein engineering of the R-spine

Articles

Structure of a tRNA-specific deaminase with compromised deamination activity

Huijuan Liu, Saibin Wu, Dewei Ran, Wei Xie

Biochem J (2020) 477 (8): 1483–1497.

https://doi.org/10.1042/BCJ20190858

Published: 29 April 2020

... to previous studies from a structural perspective. These clashes would potentially lead to reduced substrate binding affinity of McTadA, consistent with our in vitro deamination activity and binding assays. To rescue the deamination activity of McTadA, we carried out two rounds of protein engineering through...

View article titled, Structure of a tRNA-specific deaminase with compromised deamination activity

Includes: Supplementary data

Articles

Lysosomal protein thermal stability does not correlate with cellular half-life: global observations and a case study of tripeptidyl-peptidase 1

Aaron M. Collier, Yuliya Nemtsova, Narendra Kuber, Whitney Banach-Petrosky, Anurag Modak, David E. Sleat, Vikas Nanda, Peter Lobel

Biochem J (2020) 477 (3): 727–745.

https://doi.org/10.1042/BCJ20190874

Published: 14 February 2020

... tripeptidyl-peptidase 1 (TPP1). Progression of LINCL can be slowed or halted by enzyme replacement therapy, where recombinant human TPP1 is administered to patients. In this study, we utilized protein engineering techniques to increase the stability of recombinant TPP1 with the rationale that this may...

View article titled, Lysosomal protein thermal stability does not correlate with cellular half-life: global observations and a case study of tripeptidyl-peptidase 1

Includes: Supplementary data

Articles

Non-conservation of folding rates in the thioredoxin family reveals degradation of ancestral unassisted-folding

Gloria Gamiz-Arco, Valeria A. Risso, Adela M. Candel, Alvaro Inglés-Prieto, Maria L. Romero-Romero, Eric A. Gaucher, Jose A. Gavira, Beatriz Ibarra-Molero, Jose M. Sanchez-Ruiz

Biochem J (2019) 476 (23): 3631–3647.

https://doi.org/10.1042/BCJ20190739

Published: 10 December 2019

... protein engineering protein folding Instances of so-called imperfect (poor or suboptimal) ‘design’ have been extensively studied in records of evolutionary history, and have served as evidence that living organisms, rather than being designed, are the products of complex evolutionary forces...

View article titled, Non-conservation of folding rates in the thioredoxin family reveals degradation of ancestral unassisted-folding

Includes: Supplementary data

Articles

Adaptation of the Staphylococcus aureus leukocidin LukGH for the rabbit host by protein engineering

Nikolina Trstenjak, Lukas Stulik, Harald Rouha, Jakub Zmajkovic, Manuel Zerbs, Eszter Nagy, Adriana Badarau

Biochem J (2019) 476 (2): 275–292.

https://doi.org/10.1042/BCJ20180691

Published: 25 January 2019

...). Published by Portland Press Limited on behalf of the Biochemical Society 2019 leukocidin protein engineering species adaptation S. aureus Table 1 Cell-tropism and species specificity of the bi-component cytotoxins of Staphylococcus aureus Toxin Cellular receptors...

View article titled, Adaptation of the Staphylococcus aureus leukocidin LukGH for the rabbit host by protein engineering

Includes: Supplementary data

Articles

Engineering Arabidopsis long-chain acyl-CoA synthetase 9 variants with enhanced enzyme activity

Yang Xu, Kristian Mark P. Caldo, Roman Holic, Elzbieta Mietkiewska, Jocelyn Ozga, Syed Masood Rizvi, Guanqun Chen, Randall J. Weselake

Biochem J (2019) 476 (1): 151–164.

https://doi.org/10.1042/BCJ20180787

Published: 15 January 2019

.... Weselake ( [email protected] ) 1 10 2018 11 12 2018 17 12 2018 17 12 2018 Arabidopsis thaliana error-prone PCR LACS protein engineering Saccharomyces cerevisiae Fatty acids are carboxylic acids with highly reduced acyl chains, which act as the major...

View article titled, Engineering Arabidopsis long-chain acyl-CoA synthetase 9 variants with enhanced enzyme activity

Includes: Supplementary data

Articles

Rational engineering of a malate dehydrogenase for microbial production of 2,4-dihydroxybutyric acid via homoserine pathway

Cláudio J.R. Frazão, Christopher M. Topham, Yoann Malbert, Jean Marie François, Thomas Walther

Biochem J (2018) 475 (23): 3887–3901.

https://doi.org/10.1042/BCJ20180765

Published: 12 December 2018

... of the Biochemical Society 2018 homoserine malate dehydrogenase protein engineering site-directed mutagenesis synthetic biology A central challenge in the creation of a resource-efficient and sustainable bioeconomy lies in the reduction in our dependence on fossil resources and a shift in focus...

View article titled, Rational engineering of a malate dehydrogenase for microbial production of 2,4-dihydroxybutyric acid via homoserine pathway

Includes: Supplementary data

Articles

Pre-equilibrium competitive library screening for tuning inhibitor association rate and specificity toward serine proteases

Itay Cohen, Si Naftaly, Efrat Ben-Zeev, Alexandra Hockla, Evette S. Radisky, Niv Papo

Biochem J (2018) 475 (7): 1335–1352.

https://doi.org/10.1042/BCJ20180070

Published: 16 April 2018

.... directed evolution protease inhibitor protein engineering protein–protein interactions (PPIs) serine proteases Extracellular proteases that are aberrantly expressed in the tumor microenvironment are key contributors to cancer growth, progression and metastasis [ 1 – 3 ] and hence constitute...

View article titled, Pre-equilibrium competitive library screening for tuning inhibitor association rate and specificity toward serine proteases

Includes: Supplementary data

Articles

Exploring the past and the future of protein evolution with ancestral sequence reconstruction: the ‘retro’ approach to protein engineering

Yosephine Gumulya, Elizabeth M.J. Gillam

Biochem J (2017) 474 (1): 1–19.

https://doi.org/10.1042/BCJ20160507

Published: 22 December 2016

... sequences and limited phylogenetic trees. This review will focus, firstly, on the use of ASR to uncover links between sequence and phenotype and, secondly, on the practical application of ASR in protein engineering. Correspondence: Elizabeth M.J. Gillam ( [email protected] ) 27 5 2016 7 11...

View article titled, Exploring the past and the future of protein evolution with ancestral sequence reconstruction: the ‘retro’ approach to protein engineering

Articles

Engineering ancestral protein hyperstability

M. Luisa Romero-Romero, Valeria A. Risso, Sergio Martinez-Rodriguez, Beatriz Ibarra-Molero, Jose M. Sanchez-Ruiz

Biochem J (2016) 473 (20): 3611–3620.

https://doi.org/10.1042/BCJ20160532

Published: 11 October 2016

... approach to expand the capability of ancestral reconstruction to search sequence space for extreme protein properties of biotechnological interest. protein engineering protein stability resurrected proteins thioredoxin The engineering of proteins with enhanced stability has drawn...

View article titled, Engineering ancestral protein hyperstability

Articles

High-affinity Anticalins with aggregation-blocking activity directed against the Alzheimer β-amyloid peptide

Sabine Rauth, Dominik Hinz, Michael Börger, Markus Uhrig, Manuel Mayhaus, Matthias Riemenschneider, Arne Skerra

Biochem J (2016) 473 (11): 1563–1578.

https://doi.org/10.1042/BCJ20160114

Published: 27 May 2016

...) and alternative drug candidates compared with current biopharmaceutical treatments. Aβ peptide lipocalin neurodegeneration protein engineering Alzheimer's disease (AD) is the most prevalent form of dementia, with 10% of the human population older than 65 years and 40% older than 85 years affected...

View article titled, High-affinity Anticalins with aggregation-blocking activity directed against the Alzheimer β-amyloid peptide

Includes: Supplementary data

Articles

Combinatorial protein engineering of proteolytically resistant mesotrypsin inhibitors as candidates for cancer therapy

Itay Cohen, Olumide Kayode, Alexandra Hockla, Banumathi Sankaran, Derek C. Radisky, Evette S. Radisky, Niv Papo

Biochem J (2016) 473 (10): 1329–1341.

https://doi.org/10.1042/BJ20151410

Published: 11 May 2016

... enzyme inhibition mesotrypsin protease inhibitor protein engineering proteolysis X-ray structure The human serine protease mesotrypsin, encoded by the PRSS3 gene, has recently emerged as a compelling new candidate drug target in cancer. Although its specific pathological roles are yet...

View article titled, Combinatorial protein engineering of proteolytically resistant mesotrypsin inhibitors as candidates for cancer therapy

Includes: Supplementary data

Articles

Human P450-like oxidation of diverse proton pump inhibitor drugs by ‘gatekeeper’ mutants of flavocytochrome P450 BM3

Christopher F. Butler, Caroline Peet, Kirsty J. McLean, Michael T. Baynham, Richard T. Blankley, Karl Fisher, Stephen E. J. Rigby, David Leys, Michael W. Voice, Andrew W. Munro

Biochem J (2014) 460 (2): 247–259.

https://doi.org/10.1042/BJ20140030

Published: 13 May 2014

... P450 drug metabolism protein engineering proton pump inhibitor Replacing traditional synthetic routes to complex organic molecules using enzyme-catalysed reactions has many advantages. These include lower energy requirements, fewer synthetic steps and the ability to activate centres...

View article titled, Human P450-like oxidation of diverse proton pump inhibitor drugs by ‘gatekeeper’ mutants of flavocytochrome P450 BM3

Includes: Supplementary data

Articles

Structure of a class III engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity

Emily Golden, Rachel Paterson, Wan Jun Tie, Anandhi Anandan, Gavin Flematti, Gianluca Molla, Elena Rosini, Loredano Pollegioni, Alice Vrielink

Biochem J (2013) 451 (2): 217–226.

https://doi.org/10.1042/BJ20121715

Published: 28 March 2013

... deposited in the PDB (accession codes 4HSR and 4HST respectively). crystal structure enzyme activity protein engineering substrate specificity transition state analogue Cephalosporins are currently the most widely prescribed class of antibiotics on the market and are used to treat both...

View article titled, Structure of a class III engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity

Includes: Supplementary data

Articles

The P 2 ′ residue is a key determinant of mesotrypsin specificity: engineering a high-affinity inhibitor with anticancer activity

Moh'd A. Salameh, Alexei S. Soares, Alexandra Hockla, Derek C. Radisky, Evette S. Radisky

Biochem J (2011) 440 (1): 95–105.

https://doi.org/10.1042/BJ20110788

Published: 27 October 2011

... discovery endopeptidase enzyme inhibition mesotrypsin protease inhibitor protein engineering Proteases that are aberrantly expressed in the tumour microenvironment represent key contributors to tumour growth and progression [ 1 ], as well as a potentially promising category of drug targets [ 2...

View article titled, The P 2 ′ residue is a key determinant of mesotrypsin specificity: engineering a high-affinity inhibitor with anticancer activity

Articles

Substrate specificity of haloalkane dehalogenases

Tana Koudelakova, Eva Chovancova, Jan Brezovsky, Marta Monincova, Andrea Fortova, Jiri Jarkovsky, Jiri Damborsky

Biochem J (2011) 435 (2): 345–354.

https://doi.org/10.1042/BJ20101405

Published: 29 March 2011

... ). 1 9 2010 14 1 2011 4 2 2011 4 2 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 haloalkane dehalogenase phylogenetic analysis principal component analysis protein engineering substrate specificity Enzymes are biological catalysts...

View article titled, Substrate specificity of haloalkane dehalogenases

Includes: Supplementary data

Articles

How the biotin–streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer

Claire E. Chivers, Apurba L. Koner, Edward D. Lowe, Mark Howarth

Biochem J (2011) 435 (1): 55–63.

https://doi.org/10.1042/BJ20101593

Published: 15 March 2011

...://creativecommons.org/licenses/by-nc/2.5/ ) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. avidin biotin protein engineering protein–ligand interaction streptavidin traptavidin The capture of the small molecule...

View article titled, How the biotin–streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer

Includes: Supplementary data

Articles

Proteases as therapeutics

Charles S. Craik, Michael J. Page, Edwin L. Madison

Biochem J (2011) 435 (1): 1–16.

https://doi.org/10.1042/BJ20100965

Published: 15 March 2011

... © 2011 Biochemical Society 2011 procoagulant protease therapy protein degradation protein engineering trypsin fold Sequencing of the human genome revealed that more than 2% of our genes encode proteases, suggesting that these enzymes possess functions more complex than the simple...

View article titled, Proteases as therapeutics

Includes: Supplementary data

Articles

X-ray crystallographic snapshots of reaction intermediates in the G117H mutant of human butyrylcholinesterase, a nerve agent target engineered into a catalytic bioscavenger

Florian Nachon, Eugenie Carletti, Marielle Wandhammer, Yvain Nicolet, Lawrence M. Schopfer, Patrick Masson, Oksana Lockridge

Biochem J (2011) 434 (1): 73–82.

https://doi.org/10.1042/BJ20101648

Published: 27 January 2011

... that the dephosphylation mechanism involves either a substantial conformational change of the His 117 residue or an adjacent nucleophilic substitution by water. acetylcholinesterase bioscavenger butyrylcholinesterase organophosphorus protein engineering spontaneous reactivation Recent progress...

View article titled, X-ray crystallographic snapshots of reaction intermediates in the G117H mutant of human butyrylcholinesterase, a nerve agent target engineered into a catalytic bioscavenger

Articles

Evolving improved Synechococcus Rubisco functional expression in Escherichia coli

Oliver Mueller-Cajar, Spencer M. Whitney

Biochem J (2008) 414 (2): 205–214.

https://doi.org/10.1042/BJ20080668

Published: 12 August 2008

[email protected] ). 27 3 2008 2 5 2008 19 5 2008 19 5 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 chaperonin CO 2 fixation directed evolution protein engineering RbcX ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) Rubisco...

View article titled, Evolving improved Synechococcus Rubisco functional expression in Escherichia coli

Includes: Supplementary data

Articles

Artificially evolved Synechococcus PCC6301 Rubisco variants exhibit improvements in folding and catalytic efficiency

Dina N. Greene, Spencer M. Whitney, Ichiro Matsumura

Biochem J (2007) 404 (3): 517–524.

https://doi.org/10.1042/BJ20070071

Published: 29 May 2007

... protein engineering ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyses the nucleophilic carboxylation of ribulose-P 2 ( D -ribulose-1,5-bisphosphate). This conversion of inorganic CO 2 into carbohydrate is frequently...

View article titled, Artificially evolved Synechococcus PCC6301 Rubisco variants exhibit improvements in folding and catalytic efficiency

Includes: Supplementary data

Articles

Mutagenesis of solvent-exposed amino acids in Photinus pyralis luciferase improves thermostability and pH-tolerance

G. H. Erica Law, Olga A. Gandelman, Laurence C. Tisi, Christopher R. Lowe, James A. H. Murray

Biochem J (2006) 397 (2): 305–312.

https://doi.org/10.1042/BJ20051847

Published: 28 June 2006

... pyralis protein engineering Beetle luciferases catalyse the efficient transfer of chemical energy into light via a two-step process, utilizing ATP-Mg 2+ , firefly luciferin ( D -LH 2 ) and molecular oxygen, yielding oxyluciferin (LO): A wide range of novel in vitro and in vivo...

View article titled, Mutagenesis of solvent-exposed amino acids in Photinus pyralis luciferase improves thermostability and pH-tolerance

Includes: Supplementary data

Articles

Structural plasticity of the cyclic-cystine-knot framework: implications for biological activity and drug design

Richard J. Clark, Norelle L. Daly, David J. Craik

Biochem J (2006) 394 (1): 85–93.

https://doi.org/10.1042/BJ20051691

Published: 27 January 2006

... 2005 21 11 2005 21 11 2005 The Biochemical Society, London 2006 cyclic protein cyclotide haemolytic activity kalata NMR protein engineering The cyclotides [ 1 ] are a fascinating family of plant proteins that are distinguished by a head-to-tail peptide backbone...

View article titled, Structural plasticity of the cyclic-cystine-knot framework: implications for biological activity and drug design

Articles

Tetravalent single-chain avidin: from subunits to protein domains via circularly permuted avidins

Henri R. Nordlund, Vesa P. Hytönen, Jarno Hörhä, Juha A. E. Määttä, Daniel J. White, Katrin Halling, Eevaleena J. Porkka, J. Peter Slotte, Olli H. Laitinen, Markku S. Kulomaa

Biochem J (2005) 392 (3): 485–491.

https://doi.org/10.1042/BJ20051038

Published: 06 December 2005

... chain. In contrast with wild-type avidin, which contains four identical avidin monomers, scAvd enables each one of the four avidin domains to be independently modified by protein engineering. Therefore the scAvd scaffold can be used to construct spatially and stoichiometrically defined pseudotetrameric...

View article titled, Tetravalent single-chain avidin: from subunits to protein domains via circularly permuted avidins

Articles

Characterization of the ligandin site of maize glutathione S-transferase I

Irine A. AXARLI, Daniel J. RIGDEN, Nikolaos E. LABROU

Biochem J (2004) 382 (3): 885–893.

https://doi.org/10.1042/BJ20040298

Published: 07 September 2004

... 3GA glutathione S-transferase herbicide detoxification ligandin protein engineering triazine dye GSTs (glutathione S-transferases; EC 2.5.1.18) are dimeric detoxification enzymes that catalyse a wide variety of conjugations of GSH to hydrophobic electrophilic compounds [ 1 , 2 ]. Each GST...

View article titled, Characterization of the ligandin site of maize glutathione S-transferase I

Articles

Tailoring tissue inhibitor of metalloproteinases-3 to overcome the weakening effects of the cysteine-rich domains of tumour necrosis factor-alpha converting enzyme

Meng-Huee LEE, Philippa DODDS, Vandana VERMA, Klaus MASKOS, Vera KNÄUPER, Gillian MURPHY

Biochem J (2003) 371 (2): 369–376.

https://doi.org/10.1042/bj20021538

Published: 15 April 2003

... 2003 30 1 2003 30 1 2003 The Biochemical Society, London ©2003 2003 binding affinity conformation tuning protein engineering Abbreviations used: ADAM, a disintegrin and metalloproteinase; DMEM, Dulbecco's modified Eagle's medium; ECM, extracellular matrix; EGF...

View article titled, Tailoring tissue inhibitor of metalloproteinases-3 to overcome the weakening effects of the cysteine-rich domains of tumour necrosis factor-alpha converting enzyme

Articles

Organization of the multifunctional enzyme type 1: interaction between N- and C-terminal domains is required for the hydratase-1/isomerase activity

Tiila-Riikka KIEMA, Jukka P. TASKINEN, Päivi L. PIRILÄ, Kari T. KOIVURANTA, Rik K. WIERENGA, J. Kalervo HILTUNEN

Biochem J (2002) 367 (2): 433–441.

https://doi.org/10.1042/bj20020292

Published: 15 October 2002

..., (ii) stabilization of the dehydrogenation domains by interaction with the domain D and (iii) the targeting of the perMFE-1 to peroxisomes via its C-terminal tripeptide. Key words: b-oxidation, lipid metabolism, peroxisome, protein engineering, structure function. strategy of an oxyanion intermediate...

View article titled, Organization of the multifunctional enzyme type 1: interaction between N- and C-terminal domains is required for the hydratase-1/isomerase activity

Articles

The transfer of transthyretin and receptor-binding properties from the plasma retinol-binding protein to the epididymal retinoic acid-binding protein

Manickavasagam SUNDARAM, Daan M. F. van AALTEN, John B. C. FINDLAY, Asipu SIVAPRASADARAO

Biochem J (2002) 362 (2): 265–271.

https://doi.org/10.1042/bj3620265

Published: 22 February 2002

... and L-3 were found to be the major determinants of the receptor- and TTR-binding speci- ficities respectively. Thus we demonstrate that lipocalins serve as excellent scaffolds for engineering novel biological functions. Key words: vitamin A, lipocalin, protein engineering, RBP ERABP chimaera, RBP...

View article titled, The transfer of transthyretin and receptor-binding properties from the plasma retinol-binding protein to the epididymal retinoic acid-binding protein

Articles

Conserved tryptophan in cytochrome c: importance of the unique side-chain features of the indole moiety

Karen M. BLACK, Ian CLARK-LEWIS, Carmichael J. A. WALLACE

Biochem J (2001) 359 (3): 715–720.

https://doi.org/10.1042/bj3590715

Published: 25 October 2001

... 9 2001 The Biochemical Society, London ©2001 2001 non-coded amino acids protein engineering semisynthesis structure–function Biochem. J. (2001) 359, 715 720 (Printed in Great Britain) 715 Conserved tryptophan in cytochrome c : importance of the unique side-chain features...

View article titled, Conserved tryptophan in cytochrome c: importance of the unique side-chain features of the indole moiety

Articles

Cloning and expression of a single-chain catalytic antibody that acts as a glutathione peroxidase mimic with high catalytic efficiency

Xiaojun REN, Shujuan GAO, Delin YOU, Hualiang HUANG, Zi LIU, Ying MU, Junqiu LIU, Yan ZHANG, Ganglin YAN, Guimin LUO, Tongshu YANG, Jiacong SHEN

Biochem J (2001) 359 (2): 369–374.

https://doi.org/10.1042/bj3590369

Published: 08 October 2001

... words: artificial enzyme, chemical mutation, enzyme mimics, protein engineering, selenium. is highly expressed in the mucosal epithelium of the gastro- intestinal tract [9,10]. Of these forms, GSHPx-1 has been well studied [3]. The mechanism by which GSHPx-1 catalyses the breakdown of hydroperoxides has...

View article titled, Cloning and expression of a single-chain catalytic antibody that acts as a glutathione peroxidase mimic with high catalytic efficiency

Articles

Functional and structural roles of the glutathione-binding residues in maize (Zea mays) glutathione S-transferase I

Nikolaos E. LABROU, Luciane V. MELLO, Yannis D. CLONIS

Biochem J (2001) 358 (1): 101–110.

https://doi.org/10.1042/bj3580101

Published: 08 August 2001

...2001 2001 essential dynamics herbicide detoxification molecular dynamics protein engineering Abbreviations used: CDNB, 1-chloro-2,4-dinitrobenzene; G-site, GSH binding site; GST, glutathione S-transferase; H-site, electrophile binding site; Ni-NTA, Ni 2+ -nitrilotriacetate. Biochem...

View article titled, Functional and structural roles of the glutathione-binding residues in maize (Zea mays) glutathione S-transferase I

Articles

Second generation antitumour human RNase: significance of its structural and functional features for the mechanism of antitumour action

Sonia Di GAETANO, Giuseppe D'ALESSIO, Renata PICCOLI

Biochem J (2001) 358 (1): 241–247.

https://doi.org/10.1042/bj3580241

Published: 08 August 2001

... be addressed (e-mail [email protected] ). 7 3 2001 10 4 2001 11 6 2001 The Biochemical Society, London ©2001 2001 antitumour agents protein engineering ribonucleases RNase inhibitor Abbreviations used: BS-RNase, bovine seminal RNase; cRI, cytosolic RNase inhibitor; DTT...

View article titled, Second generation antitumour human RNase: significance of its structural and functional features for the mechanism of antitumour action

Articles

Active-site characterization of Candida boidinii formate dehydrogenase

Nikolaos E. LABROU, Daniel J. RIGDEN

Biochem J (2001) 354 (2): 455–463.

https://doi.org/10.1042/bj3540455

Published: 22 February 2001

... properties of His- 311 are important for enzyme function. An important structural role has also been attributed to cis-Pro-288. This residue may provide the key residues Gln-287 and His-311 with the proper orientation for productive binding of formate. Key words: kinetic mechanism, protein engineering, site...

View article titled, Active-site characterization of Candida boidinii formate dehydrogenase

Articles

Probing the NADPH-binding site of Escherichia coli flavodoxin oxidoreductase

Claire LEADBEATER, Lisa MCIVER, Dominic J. CAMPOPIANO, Scott P. WEBSTER, Robert L. BAXTER, Sharon M. KELLY, Nicholas C. PRICE, Dominikus A. LYSEK, Michael A. NOBLE, Stephen K. CHAPMAN, Andrew W. MUNRO

Biochem J (2000) 352 (2): 257–266.

https://doi.org/10.1042/bj3520257

Published: 24 November 2000

... and R184 located close to the adenosine ribose 2«-phosphate group, and R144 likely to interact with the nicotinamide ribose 5«-phosphate group. Key words: coenzyme affinity, enzyme kinetics, flavodoxin NADP+ oxidoreductase, protein engineering. with other members of the ferredoxin reductase family...

View article titled, Probing the NADPH-binding site of Escherichia coli flavodoxin oxidoreductase

Articles

The influence of secretory-protein charge on late stages of secretion from the Gram-positive bacterium Bacillus subtilis

Keith STEPHENSON, Christina L. JENSEN, Steen T. JØRGENSEN, Jeremy H. LAKEY, Colin R. HARWOOD

Biochem J (2000) 350 (1): 31–39.

https://doi.org/10.1042/bj3500031

Published: 09 August 2000

... 25 5 2000 The Biochemical Society, London © 2000 2000 protein secretion protein engineering cell walls α-amylase Biochem. J. (2000) 350, 31 39 (Printed in Great Britain) 31 The influence of secretory-protein charge on late stages of secretion from the Gram-positive bacterium...

View article titled, The influence of secretory-protein charge on late stages of secretion from the Gram-positive bacterium Bacillus subtilis

Articles

Mechanistic studies of morphine dehydrogenase and stabilization against covalent inactivation

Edward H. WALKER, Christopher E. FRENCH, Deborah A. RATHBONE, Neil C. BRUCE

Biochem J (2000) 345 (3): 687–692.

https://doi.org/10.1042/bj3450687

Published: 25 January 2000

..., 504 510 34 Ricigliano, J. W. and Penning, T. M. (1990) Biochem. J. 269, 749 755 # 2000 Biochemical Society biocatalysis dehydrogenases opiates protein engineering ...

View article titled, Mechanistic studies of morphine dehydrogenase and stabilization against covalent inactivation

Articles

Inclusion of a furin-sensitive spacer enhances the cytotoxicity of ribotoxin restrictocin containing recombinant single-chain immunotoxins

Anita GOYAL, Janendra K. BATRA

Biochem J (2000) 345 (2): 247–254.

https://doi.org/10.1042/bj3450247

Published: 10 January 2000

... . 1 To whom correspondence should be addressed (e-mail janendra@;nii.res.in or jkbatra@;yahoo.com). 12 7 1999 30 9 1999 27 10 1999 The Biochemical Society, London © 2000 2000 chimaeric toxins protease protein engineering targeting toxins Biochem. J. (2000) 345, 247...

View article titled, Inclusion of a furin-sensitive spacer enhances the cytotoxicity of ribotoxin restrictocin containing recombinant single-chain immunotoxins

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Reaction mechanism of recombinant 3-oxoacyl-(acyl-carrier-protein) synthase III from Cuphea wrightii embryo, a fatty acid synthase type II condensing enzyme

Amine ABBADI, Monika BRUMMEL, Burkhardt S. SCHüTT, Mary B. SLABAUGH, Ricardo SCHUCH, Friedrich SPENER

Biochem J (2000) 345 (1): 153–160.

https://doi.org/10.1042/bj3450153

Published: 17 December 1999

.... Key words: ACP, active-site residues, fatty acid synthesis, protein engineering. transfer of the acyl group from the pantetheine moiety of an acyl donor to the substrate-binding site on the condensing enzyme. In the condensing domain of the yeast multifunctional-fatty-acid synthase (FAS, type I...

View article titled, Reaction mechanism of recombinant 3-oxoacyl-(acyl-carrier-protein) synthase III from Cuphea wrightii embryo, a fatty acid synthase type II condensing enzyme

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An aromatic, but not a basic, residue is involved in the toxicity of group-II phospholipase A2 neurotoxins

Jože PUNGERĆAR, Igor KRIAJ, Ning-Sheng LIANG, Franc GUBENŠEK

Biochem J (1999) 341 (1): 139–145.

https://doi.org/10.1042/bj3410139

Published: 24 June 1999

... activity, protein engineering, Vipera ammodytes ammodytes. have been used, from comparison of their primary and tertiary structures, chemical modification and the use of specific site- directed antibodies to acceptor-binding studies. Despite nu- merous attempts, toxic PLA # s still remain a complex multi...

View article titled, An aromatic, but not a basic, residue is involved in the toxicity of group-II phospholipase A2 neurotoxins

Articles

Prediction and experimental testing of Bacillus acidocaldarius thioredoxin stability

Emilia PEDONE, Raffaele CANNIO, Michele SAVIANO, Mosè ROSSI, Simonetta BARTOLUCCI

Biochem J (1999) 339 (2): 309–317.

https://doi.org/10.1042/bj3390309

Published: 08 April 1999

... to denaturants such as guanidine HCl and urea was revealed; the wild-type protein always proved to be the most resistant. The results obtained show the importance of hydrogen bonds and ion pairs in determining protein stability and confirm that simulation methods are able to direct protein engineering in site...

View article titled, Prediction and experimental testing of Bacillus acidocaldarius thioredoxin stability

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