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1-46 of 46
Keywords: protein engineering
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Articles
Journal:
Biochemical Journal
Biochem J (2024) 481 (24): 2017–2036.
Published: 23 December 2024
..., the electrochemical performance of Geobacter ’s biofilms in MFCs remains challenging to implement industrially. Multiple approaches are being investigated to enhance MFC technologies. Protein engineering of multihaem cytochromes, key components of Geobacter ’s EET pathways, can, conceivably, be pursued to improve...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2023) 480 (16): 1317–1330.
Published: 25 August 2023
... and deubiquitinase activity with the OTUD1 substrate RIPK1 were inhibited. Herein we describe the development of molecular tools for exploring the activity of OTUD1 in a cellular context, towards protein-based therapeutics. deubiquitinases inhibitor phage display protein engineering ubiquitin variants UIM...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2023) 480 (14): 1097–1107.
Published: 17 July 2023
... a transformative agreement with MALMAD. auto-cleavage auto-degradation enzyme engineering matrix metalloproteases MMP-9 protein engineering Matrix metalloproteinases (MMPs) are a family of proteases, comprising more than twenty different enzymes in humans. They are composed of a catalytic domain...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2023) 480 (2): 127–140.
Published: 23 January 2023
..., and that strongly depend on the precise goals that each Synthetic Biology project pursues. Protein Engineering is also a very recent area of research, and de novo design of proteins is much increasing our repertoire of nanodevices [ 8 ], instrumental for Synthetic Biology purposes. One of the first attempts...
Articles
Journal:
Biochemical Journal
Biochem J (2021) 478 (15): 3047–3062.
Published: 13 August 2021
...). Protein was concentrated to 3 mg/ml, flash-cooled in liquid nitrogen and stored at −80°C. © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 acetyltransferases enzyme activation metalloenzymes molecular mechanisms protein engineering...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2020) 477 (9): 1701–1719.
Published: 11 May 2020
... i , and ∑ # reads mut, i is the sum of all the reads for all mutants in library fraction i . metalloproteases next-generation sequencing protease inhibitor protein engineering protein–protein interactions Protein fitness — the ability of a protein to perform its main function...
Includes: Supplementary data
Articles
Matthew J. Byrne, Nazia Nasir, Christine Basmadjian, Chitra Bhatia, Rory F. Cunnison, Katherine H. Carr, Corine Mas-Droux, Sharon Yeoh, Céline Cano, Richard Bayliss
Journal:
Biochemical Journal
Biochem J (2020) 477 (8): 1525–1539.
Published: 29 April 2020
... have previously generated active, phosphorylated Nek7 using genetically encoded phospho-Ser [ 34 ]. Unfortunately, we were unable to generate diffraction-quality crystals using this protein. We, therefore, turned to protein engineering to generate an alternative crystal form through manipulation...
Articles
Journal:
Biochemical Journal
Biochem J (2020) 477 (8): 1483–1497.
Published: 29 April 2020
... to previous studies from a structural perspective. These clashes would potentially lead to reduced substrate binding affinity of McTadA, consistent with our in vitro deamination activity and binding assays. To rescue the deamination activity of McTadA, we carried out two rounds of protein engineering through...
Includes: Supplementary data
Articles
Aaron M. Collier, Yuliya Nemtsova, Narendra Kuber, Whitney Banach-Petrosky, Anurag Modak, David E. Sleat, Vikas Nanda, Peter Lobel
Journal:
Biochemical Journal
Biochem J (2020) 477 (3): 727–745.
Published: 14 February 2020
... tripeptidyl-peptidase 1 (TPP1). Progression of LINCL can be slowed or halted by enzyme replacement therapy, where recombinant human TPP1 is administered to patients. In this study, we utilized protein engineering techniques to increase the stability of recombinant TPP1 with the rationale that this may...
Includes: Supplementary data
Articles
Gloria Gamiz-Arco, Valeria A. Risso, Adela M. Candel, Alvaro Inglés-Prieto, Maria L. Romero-Romero, Eric A. Gaucher, Jose A. Gavira, Beatriz Ibarra-Molero, Jose M. Sanchez-Ruiz
Journal:
Biochemical Journal
Biochem J (2019) 476 (23): 3631–3647.
Published: 10 December 2019
... is not possible, since position 76 is close to the catalytic disulfide bridge and the presence of a proline at that position is required for a fully functional active-site conformation [ 12 ]. Pro76 is thus strictly conserved in thioredoxins. molecular evolution protein engineering protein folding...
Includes: Supplementary data
Articles
Nikolina Trstenjak, Lukas Stulik, Harald Rouha, Jakub Zmajkovic, Manuel Zerbs, Eszter Nagy, Adriana Badarau
Journal:
Biochemical Journal
Biochem J (2019) 476 (2): 275–292.
Published: 25 January 2019
... is the adaptation of Listeria monocytogenes to mouse by improving binding of the listerial invasion protein InlA to the murine variant of its cognate receptor E-cadherin by protein engineering [ 26 ]. LukGH is the most potent leukocidin in in vitro assays and ex vivo models [ 13 , 27 – 29...
Includes: Supplementary data
Articles
Yang Xu, Kristian Mark P. Caldo, Roman Holic, Elzbieta Mietkiewska, Jocelyn Ozga, Syed Masood Rizvi, Guanqun Chen, Randall J. Weselake
Journal:
Biochemical Journal
Biochem J (2019) 476 (1): 151–164.
Published: 15 January 2019
.... Weselake ( [email protected] ) 1 10 2018 11 12 2018 17 12 2018 17 12 2018 Arabidopsis thaliana error-prone PCR LACS protein engineering Saccharomyces cerevisiae Fatty acids are carboxylic acids with highly reduced acyl chains, which act as the major...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2018) 475 (23): 3887–3901.
Published: 12 December 2018
...). Published by Portland Press Limited on behalf of the Biochemical Society 2018 homoserine malate dehydrogenase protein engineering site-directed mutagenesis synthetic biology A central challenge in the creation of a resource-efficient and sustainable bioeconomy lies in the reduction in our...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2018) 475 (7): 1335–1352.
Published: 16 April 2018
... 2018 directed evolution protease inhibitor protein engineering protein–protein interactions (PPIs) serine proteases Extracellular proteases that are aberrantly expressed in the tumor microenvironment are key contributors to cancer growth, progression and metastasis [ 1 – 3 ] and hence...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2017) 474 (1): 1–19.
Published: 22 December 2016
... sequences and limited phylogenetic trees. This review will focus, firstly, on the use of ASR to uncover links between sequence and phenotype and, secondly, on the practical application of ASR in protein engineering. Correspondence: Elizabeth M.J. Gillam ( [email protected] ) 27 5 2016 7 11...
Articles
M. Luisa Romero-Romero, Valeria A. Risso, Sergio Martinez-Rodriguez, Beatriz Ibarra-Molero, Jose M. Sanchez-Ruiz
Journal:
Biochemical Journal
Biochem J (2016) 473 (20): 3611–3620.
Published: 11 October 2016
... to infinite scan rate (in practice to 1/scan rate = 0) should correct the effect of any, presumably small, kinetic distortion on the outcome of the pseudo-two-state fits to the truncated profiles. Such extrapolations are described below. protein engineering protein stability resurrected proteins...
Articles
Sabine Rauth, Dominik Hinz, Michael Börger, Markus Uhrig, Manuel Mayhaus, Matthias Riemenschneider, Arne Skerra
Journal:
Biochemical Journal
Biochem J (2016) 473 (11): 1563–1578.
Published: 27 May 2016
... neurodegeneration protein engineering Alzheimer's disease (AD) is the most prevalent form of dementia, with 10% of the human population older than 65 years and 40% older than 85 years affected [ 1 ]. Apart from certain forms of inherited AD [ 2 ], age is the major risk factor associated...
Includes: Supplementary data
Articles
Itay Cohen, Olumide Kayode, Alexandra Hockla, Banumathi Sankaran, Derek C. Radisky, Evette S. Radisky, Niv Papo
Journal:
Biochemical Journal
Biochem J (2016) 473 (10): 1329–1341.
Published: 11 May 2016
..., an enzyme that contributes to progression and metastasis of lung, prostate, breast and pancreatic cancers. cancer therapy directed evolution enzyme inhibition mesotrypsin protease inhibitor protein engineering proteolysis X-ray structure Our unique screening strategy, designated ‘triple...
Includes: Supplementary data
Articles
Christopher F. Butler, Caroline Peet, Kirsty J. McLean, Michael T. Baynham, Richard T. Blankley, Karl Fisher, Stephen E. J. Rigby, David Leys, Michael W. Voice, Andrew W. Munro
Journal:
Biochemical Journal
Biochem J (2014) 460 (2): 247–259.
Published: 13 May 2014
... were shown to catalyse oxidation of several proton pump inhibitor drugs, and to generate products identical to those formed by major human drug metabolizing P450s. crystal structure cytochrome P450 drug metabolism protein engineering proton pump inhibitor Replacing traditional...
Includes: Supplementary data
Articles
Emily Golden, Rachel Paterson, Wan Jun Tie, Anandhi Anandan, Gavin Flematti, Gianluca Molla, Elena Rosini, Loredano Pollegioni, Alice Vrielink
Journal:
Biochemical Journal
Biochem J (2013) 451 (2): 217–226.
Published: 28 March 2013
... ]. crystal structure enzyme activity protein engineering substrate specificity transition state analogue GAs have been divided into five classes on the basis of substrate specificity and sequence conservation [ 5 , 10 , 11 ]. To date, only the 3D (three-dimensional) structure of protein members...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2011) 440 (1): 95–105.
Published: 27 October 2011
... 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 crystal structure drug discovery endopeptidase enzyme inhibition mesotrypsin protease inhibitor protein engineering Proteases that are aberrantly expressed in the tumour microenvironment represent key...
Articles
Tana Koudelakova, Eva Chovancova, Jan Brezovsky, Marta Monincova, Andrea Fortova, Jiri Jarkovsky, Jiri Damborsky
Journal:
Biochemical Journal
Biochem J (2011) 435 (2): 345–354.
Published: 29 March 2011
... analysis protein engineering substrate specificity Enzymes are biological catalysts that are essential components of every biological system and are valuable in biotechnology. The key functional characteristics of an enzyme are its catalytic activity towards different substrates and its substrate...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2011) 435 (1): 55–63.
Published: 15 March 2011
...://creativecommons.org/licenses/by-nc/2.5/ ) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. avidin biotin protein engineering protein–ligand interaction streptavidin traptavidin The capture of the small molecule...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2011) 435 (1): 1–16.
Published: 15 March 2011
... 2010 10 12 2010 © The Authors Journal compilation © 2011 Biochemical Society 2011 procoagulant protease therapy protein degradation protein engineering trypsin fold In an indirect route to protease therapy, stimulation of endogenous protease activity by protein cofactors...
Includes: Supplementary data
Articles
Florian Nachon, Eugenie Carletti, Marielle Wandhammer, Yvain Nicolet, Lawrence M. Schopfer, Patrick Masson, Oksana Lockridge
Journal:
Biochemical Journal
Biochem J (2011) 434 (1): 73–82.
Published: 27 January 2011
... that the dephosphylation mechanism involves either a substantial conformational change of the His 117 residue or an adjacent nucleophilic substitution by water. acetylcholinesterase bioscavenger butyrylcholinesterase organophosphorus protein engineering spontaneous reactivation Recent progress...
Articles
Journal:
Biochemical Journal
Biochem J (2008) 414 (2): 205–214.
Published: 12 August 2008
... protein engineering RbcX ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) Rubisco [ribulose-P 2 (D-ribulose-1,5-bisphosphate) carboxylase/oxygenase; EC 4.1.1.39] is the enzyme employed by almost all photoautotrophic organisms to initiate the fixation of CO 2 into biomass via the CBB...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2007) 404 (3): 517–524.
Published: 29 May 2007
... metabolic engineering protein engineering ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyses the nucleophilic carboxylation of ribulose-P 2 ( D -ribulose-1,5-bisphosphate). This conversion of inorganic CO 2...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2006) 397 (2): 305–312.
Published: 28 June 2006
... luciferase imaging Photinus pyralis protein engineering Beetle luciferases catalyse the efficient transfer of chemical energy into light via a two-step process, utilizing ATP-Mg 2+ , firefly luciferin ( D -LH 2 ) and molecular oxygen, yielding oxyluciferin (LO): A wide range of novel...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2006) 394 (1): 85–93.
Published: 27 January 2006
... 2005 21 11 2005 21 11 2005 The Biochemical Society, London 2006 cyclic protein cyclotide haemolytic activity kalata NMR protein engineering Human type A erythrocytes were washed with PBS and centrifuged at 150 g for 30 s in a microcentrifuge several times until...
Articles
Henri R. Nordlund, Vesa P. Hytönen, Jarno Hörhä, Juha A. E. Määttä, Daniel J. White, Katrin Halling, Eevaleena J. Porkka, J. Peter Slotte, Olli H. Laitinen, Markku S. Kulomaa
Journal:
Biochemical Journal
Biochem J (2005) 392 (3): 485–491.
Published: 06 December 2005
... chain. In contrast with wild-type avidin, which contains four identical avidin monomers, scAvd enables each one of the four avidin domains to be independently modified by protein engineering. Therefore the scAvd scaffold can be used to construct spatially and stoichiometrically defined pseudotetrameric...
Articles
Journal:
Biochemical Journal
Biochem J (2004) 382 (3): 885–893.
Published: 07 September 2004
... To whom correspondence should be addressed (email [email protected] ). 24 2 2004 25 5 2004 11 6 2004 15 6 2004 The Biochemical Society, London 2004 Cibacron Blue 3GA glutathione S-transferase herbicide detoxification ligandin protein engineering triazine dye...
Articles
Journal:
Biochemical Journal
Biochem J (2003) 371 (2): 369–376.
Published: 15 April 2003
... 2003 30 1 2003 30 1 2003 The Biochemical Society, London ©2003 2003 binding affinity conformation tuning protein engineering Abbreviations used: ADAM, a disintegrin and metalloproteinase; DMEM, Dulbecco's modified Eagle's medium; ECM, extracellular matrix; EGF...
Articles
Tiila-Riikka KIEMA, Jukka P. TASKINEN, Päivi L. PIRILÄ, Kari T. KOIVURANTA, Rik K. WIERENGA, J. Kalervo HILTUNEN
Journal:
Biochemical Journal
Biochem J (2002) 367 (2): 433–441.
Published: 15 October 2002
...; mfeHAD, HAD part of MFE-1; perMFE-1, mammalian peroxisomal MFE-1; PTS-1, peroxisomal targeting signal type 1. β-oxidation lipid metabolism peroxisome protein engineering structure–function The Biochemical Society, London ©2002 2002 19 2 2002 18 6 2002 10 7 2002 10...
Articles
Journal:
Biochemical Journal
Biochem J (2002) 362 (2): 265–271.
Published: 22 February 2002
... The Biochemical Society, London ©2002 2002 vitamin A lipocalin protein engineering RBP–ERABP chimaera RBP receptor Abbreviations used: ERABP, epididymal retinoic acid-binding protein; RBP, retinol-binding protein; TTR, transthyretin; PEG 8000, poly(ethylene glycol) 8000. Biochem. J. (2002...
Articles
Journal:
Biochemical Journal
Biochem J (2001) 359 (3): 715–720.
Published: 25 October 2001
... from those of abolishing hydrogen-bond donor potential. This suggests that Trp-59 has both a local and a global stability effect by solvating a buried charge and by having a key role in the packing of the cytochrome c hydrophobic core. Key words: non-coded amino acids, protein engineering, semi...
Articles
Xiaojun REN, Shujuan GAO, Delin YOU, Hualiang HUANG, Zi LIU, Ying MU, Junqiu LIU, Yan ZHANG, Ganglin YAN, Guimin LUO, Tongshu YANG, Jiacong SHEN
Journal:
Biochemical Journal
Biochem J (2001) 359 (2): 369–374.
Published: 08 October 2001
... Society, London ©2001 2001 artificial enzyme chemical mutation enzyme mimics protein engineering selenium Abbreviations used: 2F3-scFv, single-chain Fv fragment of monoclonal antibody 2F3; GPX, glutathione peroxidase; GSHPx-1, classical cellular GPX; IMAC, immobilized metal-affinity...
Articles
Journal:
Biochemical Journal
Biochem J (2001) 358 (1): 101–110.
Published: 08 August 2001
...2001 2001 essential dynamics herbicide detoxification molecular dynamics protein engineering Abbreviations used: CDNB, 1-chloro-2,4-dinitrobenzene; G-site, GSH binding site; GST, glutathione S-transferase; H-site, electrophile binding site; Ni-NTA, Ni 2+ -nitrilotriacetate. Biochem...
Articles
Journal:
Biochemical Journal
Biochem J (2001) 358 (1): 241–247.
Published: 08 August 2001
... be addressed (e-mail [email protected] ). 7 3 2001 10 4 2001 11 6 2001 The Biochemical Society, London ©2001 2001 antitumour agents protein engineering ribonucleases RNase inhibitor Abbreviations used: BS-RNase, bovine seminal RNase; cRI, cytosolic RNase inhibitor; DTT...
Articles
Journal:
Biochemical Journal
Biochem J (2001) 354 (2): 455–463.
Published: 22 February 2001
... protein engineering site-directed mutagenesis Biochem. J. (2001) 354, 455 463 (Printed in Great Britain) 455 Active-site characterization of Candida boidinii formate dehydrogenase Nikolaos E. LABROU*1 and Daniel J. RIGDEN *Laboratory of Enzyme Technology, Department of Agricultural Biotechnology...
Articles
Claire LEADBEATER, Lisa MCIVER, Dominic J. CAMPOPIANO, Scott P. WEBSTER, Robert L. BAXTER, Sharon M. KELLY, Nicholas C. PRICE, Dominikus A. LYSEK, Michael A. NOBLE, Stephen K. CHAPMAN, Andrew W. MUNRO
Journal:
Biochemical Journal
Biochem J (2000) 352 (2): 257–266.
Published: 24 November 2000
...-mail [email protected] ). 24 3 2000 12 9 2000 28 9 2000 The Biochemical Society, London © 2000 2000 coenzyme affinity enzyme kinetics flavodoxin NADP + oxidoreductase protein engineering Biochem. J. (2000) 352, 257 266 (Printed in Great Britain) 257 Probing the NADPH...
Articles
Journal:
Biochemical Journal
Biochem J (2000) 350 (1): 31–39.
Published: 09 August 2000
... 25 5 2000 The Biochemical Society, London © 2000 2000 protein secretion protein engineering cell walls α-amylase Biochem. J. (2000) 350, 31 39 (Printed in Great Britain) 31 The influence of secretory-protein charge on late stages of secretion from the Gram-positive bacterium...
Articles
Journal:
Biochemical Journal
Biochem J (2000) 345 (3): 687–692.
Published: 25 January 2000
..., 504 510 34 Ricigliano, J. W. and Penning, T. M. (1990) Biochem. J. 269, 749 755 # 2000 Biochemical Society biocatalysis dehydrogenases opiates protein engineering ...
Articles
Journal:
Biochemical Journal
Biochem J (2000) 345 (2): 247–254.
Published: 10 January 2000
... of the location of the ligand on the toxin and they were found to release the restrictocin fragment efficiently on proteolysis in itro. Key words: chimaeric toxins, protease, protein engineering, targeting, toxins. an intervening specific protease recognition site made them cytotoxic [12,13]. Chimaeric toxins...
Articles
Amine ABBADI, Monika BRUMMEL, Burkhardt S. SCHüTT, Mary B. SLABAUGH, Ricardo SCHUCH, Friedrich SPENER
Journal:
Biochemical Journal
Biochem J (2000) 345 (1): 153–160.
Published: 17 December 1999
... scanning revealed the involvement of Arg and Arg in KAS III catalysis. The results offer for the first time a detailed mechanism for a condensing reaction catalysed by a FAS type II condensing enzyme. Key words: ACP, active-site residues, fatty acid synthesis, protein engineering. transfer of the acyl...
Articles
Journal:
Biochemical Journal
Biochem J (1999) 341 (1): 139–145.
Published: 24 June 1999
...). 4 1 1999 30 3 1999 23 4 1999 The Biochemical Society, London © 1999 1999 acceptor-binding affinity ammodytoxin enzyme activity protein engineering Vipera ammodytes ammodytes Biochem. J. (1999) 341, 139 145 (Printed in Great Britain) 139 An aromatic, but not a basic...
Articles
Journal:
Biochemical Journal
Biochem J (1999) 339 (2): 309–317.
Published: 08 April 1999
... to denaturants such as guanidine HCl and urea was revealed; the wild-type protein always proved to be the most resistant. The results obtained show the importance of hydrogen bonds and ion pairs in determining protein stability and confirm that simulation methods are able to direct protein engineering in site...
