1-42 of 42
Keywords: protein engineering
Close
Follow your search
Access your saved searches in your account

Would you like to receive an alert when new items match your search?
Close Modal
Sort by
Articles
Biochem J (2021) 478 (15): 3047–3062.
Published: 13 August 2021
... enzyme activation metalloenzymes molecular mechanisms protein engineering Metals are present as structural features in over one-third of proteins, underscoring their importance for protein structure and function [ 1 , 2 ]. The incorporation of divalent metal ions into protein structures...
Includes: Supplementary data
Articles
Biochem J (2020) 477 (9): 1701–1719.
Published: 11 May 2020
... ( papo@bgu.ac.il ) 3 3 2020 11 4 2020 14 4 2020 15 4 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 metalloproteases next-generation sequencing protease inhibitor protein engineering protein–protein...
Includes: Supplementary data
Articles
Biochem J (2020) 477 (8): 1525–1539.
Published: 29 April 2020
... and distributed under the Creative Commons Attribution License 4.0 (CC BY) . kinases protein engineering small molecules The NIMA-related kinases (Neks) are a family of Ser/Thr protein kinases, first discovered in Aspergillus nidulans that are conserved in eukaryotes [ 1 ]. Nek7 is 1 of 11 Neks...
Articles
Biochem J (2020) 477 (8): 1483–1497.
Published: 29 April 2020
... to previous studies from a structural perspective. These clashes would potentially lead to reduced substrate binding affinity of McTadA, consistent with our in vitro deamination activity and binding assays. To rescue the deamination activity of McTadA, we carried out two rounds of protein engineering through...
Includes: Supplementary data
Articles
Biochem J (2020) 477 (3): 727–745.
Published: 14 February 2020
... tripeptidyl-peptidase 1 (TPP1). Progression of LINCL can be slowed or halted by enzyme replacement therapy, where recombinant human TPP1 is administered to patients. In this study, we utilized protein engineering techniques to increase the stability of recombinant TPP1 with the rationale that this may...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (23): 3631–3647.
Published: 10 December 2019
... protein engineering protein folding Instances of so-called imperfect (poor or suboptimal) ‘design’ have been extensively studied in records of evolutionary history, and have served as evidence that living organisms, rather than being designed, are the products of complex evolutionary forces...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (2): 275–292.
Published: 25 January 2019
... address: EveliQure Biotechnologies, Helmut-Qualtinger-Gasse 2, Vienna, Austria. 24 8 2018 12 12 2018 14 12 2018 17 12 2018 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 leukocidin protein engineering species...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (1): 151–164.
Published: 15 January 2019
... of LACS with the downstream lipid biosynthetic enzymes [ 22 , 23 ]. In these regards, further improvement of the enzyme activity of LACS via protein engineering might represent novel perspectives for engineering lipid production in oleaginous organisms. Indeed, overexpression of cDNAs encoding improved...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (23): 3887–3901.
Published: 12 December 2018
... of the Biochemical Society 2018 homoserine malate dehydrogenase protein engineering site-directed mutagenesis synthetic biology We recently identified enzymes displaying HMS transaminase and OHB reductase activities, but which however possessed low affinities towards the corresponding substrates...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (7): 1335–1352.
Published: 16 April 2018
... mechanisms by which mesotrypsin drives cancer progression and for understanding the basis of target specificity and proteolytic resistance of inhibitors to proteolytic enzymes in general and serine proteases in particular. directed evolution protease inhibitor protein engineering protein–protein...
Includes: Supplementary data
Articles
Biochem J (2017) 474 (1): 1–19.
Published: 22 December 2016
... sequences and limited phylogenetic trees. This review will focus, firstly, on the use of ASR to uncover links between sequence and phenotype and, secondly, on the practical application of ASR in protein engineering. Correspondence: Elizabeth M.J. Gillam ( e.gillam@uq.edu.au ) 27 5 2016 7 11...
Articles
Biochem J (2016) 473 (20): 3611–3620.
Published: 11 October 2016
... 2016 15 8 2016 15 8 2016 © 2016 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2016 protein engineering protein stability resurrected proteins thioredoxin The engineering of proteins with enhanced stability has drawn considerable...
Articles
Biochem J (2016) 473 (11): 1563–1578.
Published: 27 May 2016
... neurodegeneration protein engineering Alzheimer's disease (AD) is the most prevalent form of dementia, with 10% of the human population older than 65 years and 40% older than 85 years affected [ 1 ]. Apart from certain forms of inherited AD [ 2 ], age is the major risk factor associated...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (10): 1329–1341.
Published: 11 May 2016
..., prostate, breast and pancreatic cancers. cancer therapy directed evolution enzyme inhibition mesotrypsin protease inhibitor protein engineering proteolysis X-ray structure 25 1 2016 29 2 2016 8 3 2016 8 3 2016 Cells, reagents and additional methods...
Includes: Supplementary data
Articles
Biochem J (2014) 460 (2): 247–259.
Published: 13 May 2014
...-oxygenase were shown to catalyse oxidation of several proton pump inhibitor drugs, and to generate products identical to those formed by major human drug metabolizing P450s. crystal structure cytochrome P450 drug metabolism protein engineering proton pump inhibitor Replacing traditional...
Includes: Supplementary data
Articles
Biochem J (2013) 451 (2): 217–226.
Published: 28 March 2013
... X-ray structure of the class III acylase: the structure of the wild-type VAC has been determined to 2.1 Å resolution, whereas the SeMet H57βS/H70βS engineered mutant was determined and refined to 1.57 Å resolution. crystal structure enzyme activity protein engineering substrate specificity...
Includes: Supplementary data
Articles
Biochem J (2011) 440 (1): 95–105.
Published: 27 October 2011
... mesotrypsin protease inhibitor protein engineering Proteases that are aberrantly expressed in the tumour microenvironment represent key contributors to tumour growth and progression [ 1 ], as well as a potentially promising category of drug targets [ 2 , 3 ]. However, a major challenge...
Articles
Biochem J (2011) 435 (2): 345–354.
Published: 29 March 2011
... ). 1 9 2010 14 1 2011 4 2 2011 4 2 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 haloalkane dehalogenase phylogenetic analysis principal component analysis protein engineering substrate specificity Enzymes are biological catalysts...
Includes: Supplementary data
Articles
Biochem J (2011) 435 (1): 55–63.
Published: 15 March 2011
...://creativecommons.org/licenses/by-nc/2.5/ ) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. avidin biotin protein engineering protein–ligand interaction streptavidin traptavidin The capture of the small molecule...
Includes: Supplementary data
Articles
Biochem J (2011) 435 (1): 1–16.
Published: 15 March 2011
... © 2011 Biochemical Society 2011 procoagulant protease therapy protein degradation protein engineering trypsin fold FVIIa belongs to a subset of proteases whose active form presents a non-canonical active-site cleft that allow them to evade interaction with endogenous inhibitors...
Includes: Supplementary data
Articles
Biochem J (2011) 434 (1): 73–82.
Published: 27 January 2011
... The Authors Journal compilation © 2011 Biochemical Society 2011 acetylcholinesterase bioscavenger butyrylcholinesterase organophosphorus protein engineering spontaneous reactivation Recent progress in the understanding of enzyme catalysis, through the use of structural biology and molecular...
Articles
Biochem J (2008) 414 (2): 205–214.
Published: 12 August 2008
... 5 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 chaperonin CO 2 fixation directed evolution protein engineering RbcX ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) Rubisco [ribulose-P 2 (D-ribulose-1,5-bisphosphate) carboxylase...
Includes: Supplementary data
Articles
Biochem J (2007) 404 (3): 517–524.
Published: 29 May 2007
... to this work. 2 To whom correspondence should be addressed (email imatsum@emory.edu ). 11 1 2007 12 3 2007 21 3 2007 21 3 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 CO 2 fixation directed evolution metabolic engineering protein...
Includes: Supplementary data
Articles
Biochem J (2006) 397 (2): 305–312.
Published: 28 June 2006
... will be superior to wild-type luciferase for many in vitro and in vivo applications. bioluminescence assay firefly luciferase imaging Photinus pyralis protein engineering Beetle luciferases catalyse the efficient transfer of chemical energy into light via a two-step process, utilizing ATP-Mg 2...
Includes: Supplementary data
Articles
Biochem J (2006) 394 (1): 85–93.
Published: 27 January 2006
.... This finding confirms the tolerance of the cyclotide framework to residue substitutions and opens up possibilities for the substitution of biologically active peptide epitopes into the framework. cyclic protein cyclotide haemolytic activity kalata NMR protein engineering 1 To whom...
Articles
Biochem J (2005) 392 (3): 485–491.
Published: 06 December 2005
... chain. In contrast with wild-type avidin, which contains four identical avidin monomers, scAvd enables each one of the four avidin domains to be independently modified by protein engineering. Therefore the scAvd scaffold can be used to construct spatially and stoichiometrically defined pseudotetrameric...
Articles
Biochem J (2004) 382 (3): 885–893.
Published: 07 September 2004
... glutathione S-transferase herbicide detoxification ligandin protein engineering triazine dye GSTs (glutathione S-transferases; EC 2.5.1.18) are dimeric detoxification enzymes that catalyse a wide variety of conjugations of GSH to hydrophobic electrophilic compounds [ 1 , 2 ]. Each GST monomer has...
Articles
Biochem J (2003) 371 (2): 369–376.
Published: 15 April 2003
... 2003 30 1 2003 30 1 2003 The Biochemical Society, London ©2003 2003 binding affinity conformation tuning protein engineering Abbreviations used: ADAM, a disintegrin and metalloproteinase; DMEM, Dulbecco's modified Eagle's medium; ECM, extracellular matrix; EGF...
Articles
Biochem J (2002) 367 (2): 433–441.
Published: 15 October 2002
..., (ii) stabilization of the dehydrogenation domains by interaction with the domain D and (iii) the targeting of the perMFE-1 to peroxisomes via its C-terminal tripeptide. Key words: b-oxidation, lipid metabolism, peroxisome, protein engineering, structure function. strategy of an oxyanion intermediate...
Articles
Biochem J (2002) 362 (2): 265–271.
Published: 22 February 2002
... The Biochemical Society, London ©2002 2002 vitamin A lipocalin protein engineering RBP–ERABP chimaera RBP receptor Abbreviations used: ERABP, epididymal retinoic acid-binding protein; RBP, retinol-binding protein; TTR, transthyretin; PEG 8000, poly(ethylene glycol) 8000. Biochem. J. (2002...
Articles
Biochem J (2001) 359 (3): 715–720.
Published: 25 October 2001
... from those of abolishing hydrogen-bond donor potential. This suggests that Trp-59 has both a local and a global stability effect by solvating a buried charge and by having a key role in the packing of the cytochrome c hydrophobic core. Key words: non-coded amino acids, protein engineering, semi...
Articles
Biochem J (2001) 359 (2): 369–374.
Published: 08 October 2001
... words: artificial enzyme, chemical mutation, enzyme mimics, protein engineering, selenium. is highly expressed in the mucosal epithelium of the gastro- intestinal tract [9,10]. Of these forms, GSHPx-1 has been well studied [3]. The mechanism by which GSHPx-1 catalyses the breakdown of hydroperoxides has...
Articles
Biochem J (2001) 358 (1): 101–110.
Published: 08 August 2001
...2001 2001 essential dynamics herbicide detoxification molecular dynamics protein engineering Abbreviations used: CDNB, 1-chloro-2,4-dinitrobenzene; G-site, GSH binding site; GST, glutathione S-transferase; H-site, electrophile binding site; Ni-NTA, Ni 2+ -nitrilotriacetate. Biochem...
Articles
Biochem J (2001) 358 (1): 241–247.
Published: 08 August 2001
... be addressed (e-mail piccoli@unina.it ). 7 3 2001 10 4 2001 11 6 2001 The Biochemical Society, London ©2001 2001 antitumour agents protein engineering ribonucleases RNase inhibitor Abbreviations used: BS-RNase, bovine seminal RNase; cRI, cytosolic RNase inhibitor; DTT...
Articles
Biochem J (2001) 354 (2): 455–463.
Published: 22 February 2001
... protein engineering site-directed mutagenesis Biochem. J. (2001) 354, 455 463 (Printed in Great Britain) 455 Active-site characterization of Candida boidinii formate dehydrogenase Nikolaos E. LABROU*1 and Daniel J. RIGDEN‹ *Laboratory of Enzyme Technology, Department of Agricultural Biotechnology...
Articles
Biochem J (2000) 352 (2): 257–266.
Published: 24 November 2000
..., enzyme kinetics, flavodoxin NADP+ oxidoreductase, protein engineering. with other members of the ferredoxin reductase family of enzymes, including spinach ferredoxin NADP+ oxidoreductase (FNR) [10], corn nitrate reductase [11] and porcine cytochrome b & reductase [12]. The FLDR structure reveals...
Articles
Biochem J (2000) 350 (1): 31–39.
Published: 09 August 2000
... 25 5 2000 The Biochemical Society, London © 2000 2000 protein secretion protein engineering cell walls α-amylase Biochem. J. (2000) 350, 31 39 (Printed in Great Britain) 31 The influence of secretory-protein charge on late stages of secretion from the Gram-positive bacterium...
Articles
Biochem J (2000) 345 (3): 687–692.
Published: 25 January 2000
... biocatalysis dehydrogenases opiates protein engineering Biochem. J. (2000) 345, 687 692 (Printed in Great Britain) 687 Mechanistic studies of morphine dehydrogenase and stabilization against covalent inactivation Edward H. WALKER1, Christopher E. FRENCH2, Deborah A. RATHBONE and Neil C. BRUCE3 Institute...
Articles
Biochem J (2000) 345 (2): 247–254.
Published: 10 January 2000
... . 1 To whom correspondence should be addressed (e-mail janendra@;nii.res.in or jkbatra@;yahoo.com). 12 7 1999 30 9 1999 27 10 1999 The Biochemical Society, London © 2000 2000 chimaeric toxins protease protein engineering targeting toxins Biochem. J. (2000) 345, 247...
Articles
Biochem J (2000) 345 (1): 153–160.
Published: 17 December 1999
... synthesis protein engineering Biochem. J. (2000) 345, 153 160 (Printed in Great Britain) 153 Reaction mechanism of recombinant 3-oxoacyl-(acyl-carrier-protein) synthase III from Cuphea wrightii embryo, a fatty acid synthase type II condensing enzyme Amine ABBADI*, Monika BRUMMEL*, Burkhardt S. SCHU$ TT...
Articles
Biochem J (1999) 341 (1): 139–145.
Published: 24 June 1999
...). 4 1 1999 30 3 1999 23 4 1999 The Biochemical Society, London © 1999 1999 acceptor-binding affinity ammodytoxin enzyme activity protein engineering Vipera ammodytes ammodytes Biochem. J. (1999) 341, 139 145 (Printed in Great Britain) 139 An aromatic, but not a basic...
Articles
Biochem J (1999) 339 (2): 309–317.
Published: 08 April 1999
... to denaturants such as guanidine HCl and urea was revealed; the wild-type protein always proved to be the most resistant. The results obtained show the importance of hydrogen bonds and ion pairs in determining protein stability and confirm that simulation methods are able to direct protein engineering in site...