... PRMT3 protein arginine methyltransferase protein evolution protein-protein interactions zinc finger ZNF200 Protein arginine methyltransferase 3 (PRMT3) is a type I arginine methyltransferase which generates the asymmetric dimethylation modifications at the arginine residues of the substrate...

... reconstruction caspase computational biology functional divergence protein evolution The caspase family of proteases offers an attractive model for examining protein evolution because a common protein scaffold (the caspase-hemoglobinase fold) was used to develop subfamilies that differ in oligomeric...

... at excitation and emission wavelengths of 428 and 475 nm, respectively, using a PerkinElmer LS-55 spectrofluorometer. allosteric regulation cation transporter enzyme kinetics membrane-bound pyrophosphatase protein evolution pyrophosphate Membrane-bound pyrophosphatases couple the hydrolysis...

... The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 enzyme kinetics lysine biosynthesis oligomerisation protein evolution Enzymes of the diaminopimelate (DAP) pathway of lysine biosynthesis are of interest as metabolic engineering targets...

... and the evolution of proteins and macromolecular complexes. Recently, however, ASR has developed from a tool for testing hypotheses about protein evolution to a useful means for designing novel proteins. The strength of this approach lies in the ability to infer ancestral sequences encoding proteins that have...

... isolated from E. coli transformed with the cloning vector only. membrane transport protein evolution proton transport pyrophosphatase sodium transport Membrane-bound pyrophosphatases (mPPases), which are present in plants, bacteria, archaea and protists, hydrolyze pyrophosphate...

... membrane transport protein evolution proton pump proton transport pyrophosphatase Membrane-bound pyrophosphatases (mPPases; Transporter Classification Database number 3.A.10) couple pyrophosphate (PP i ) hydrolysis to transmembrane transport of H + , Na + or both against their electrochemical...

..., protein structure–function and molecular evolution. However, the last several years have seen some exciting developments in combining these approaches to obtain an in-depth understanding of how proteins evolve. For example, a better understanding of how structural constraints affect protein evolution...

[email protected] ). 20 1 2012 23 4 2012 26 4 2012 26 4 2012 © The Authors Journal compilation © 2012 Biochemical Society 2012 epistasis evolutionary trajectories fitness landscape multivariate data analysis protein evolution substrate selectivity Attention has been...

... medium, provided the original work is properly cited. atomic force microscopy (AFM) mutational effect protein evolution protein stability single-molecule analysis David Rodriguez-Larrea prepared the variants from the combinatorial library and designed, performed and analysed...

... displays unusually high hydrolytic activity, but still low compared with α-amylases [ 10 ]. amylase cyclodextrin glucanotransferase (CGTase) protein evolution protein stability saccharide side reaction Figure 1 Schematic representation of the four reactions catalysed by CGTase...

... in mRNAs from both species. These results suggest that both mRNA populations and translation efficiency changed during the amphidiploidization responsible for generation of N. tabacum . This scheme could reflect a molecular mechanism of protein evolution in plants. 1 To whom correspondence should...

... 3 2007 7 3 2007 The Authors Journal compilation, The Biochemical Society, London 2007 chloroplast evolutionary tinkering Nicotiana protein evolution transcription factor transit peptide Chloroplasts are of endosymbiotic origin and derive from a cyanobacterium-like...