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Articles

The role of eIF2 phosphorylation in cell and organismal physiology: new roles for well-known actors

Xuemin Wang, Christopher G. Proud

Biochem J (2022) 479 (10): 1059–1082.

DOI: https://doi.org/10.1042/BCJ20220068

Published: 23 May 2022

... is catalysed by any one of four protein kinases, which are generally activated in response to stresses. They form a key arm of the integrated stress response (ISR). Phosphorylated eIF2 inhibits eIF2B (the protein that promotes exchange of eIF2-bound GDP for GTP) and thus impairs general protein synthesis...

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Articles

Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signalling networks using SILAC-based phosphoproteomics

Dominic P. Byrne, Christopher J. Clarke, Philip J. Brownridge, Anton Kalyuzhnyy, Simon Perkins, Amy Campbell, David Mason, Andrew R. Jones, Patrick A. Eyers, Claire E. Eyers

Biochem J (2020) 477 (13): 2451–2475.

DOI: https://doi.org/10.1042/BCJ20200309

Published: 02 July 2020

... a transformative agreement with JISC. inhibitor phosphoproteomics PLK4 protein kinase signalling substrate specificity Polo-like kinases (PLKs) are key cell cycle Ser/Thr kinases conserved in metazoan organisms. Four kinase-domain-containing PLK family members are found in most kinomes, and each...

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Conservation of structure, function and inhibitor binding in UNC-51-like kinase 1 and 2 (ULK1/2)

In Collection

Editors' pick of research highlights published in the Biochemical Journal in 2018 & 2019

Apirat Chaikuad, Sebastian E. Koschade, Alexandra Stolz, Katarina Zivkovic, Christian Pohl, Shabnam Shaid, Huiyu Ren, Lester J. Lambert, Nicholas D.P. Cosford, Christian H. Brandts, Stefan Knapp

Biochem J (2019) 476 (5): 875–887.

DOI: https://doi.org/10.1042/BCJ20190038

Published: 12 March 2019

... The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 autophagy crystallography kinase inhibitor protein kinase ULK1 ULK2 Autophagy is an essential mechanism for the survival of eukaryotic cells upon low nutrient availability and prolonged starvation...

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Development of phospho-specific Rab protein antibodies to monitor in vivo activity of the LRRK2 Parkinson's disease kinase

In Collection

Editors' pick of research highlights published in the Biochemical Journal in 2018 & 2019

Pawel Lis, Sophie Burel, Martin Steger, Matthias Mann, Fiona Brown, Federico Diez, Francesca Tonelli, Janice L. Holton, Philip Winglok Ho, Shu-Leong Ho, Meng-Yun Chou, Nicole K. Polinski, Terina N. Martinez, Paul Davies, Dario R. Alessi

Biochem J (2018) 475 (1): 1–22.

DOI: https://doi.org/10.1042/BCJ20170802

Published: 02 January 2018

... kinase 2) protein kinase predispose to Parkinson's disease, suggesting that LRRK2 inhibitors might have therapeutic benefit. Recent work has revealed that LRRK2 phosphorylates a subgroup of 14 Rab proteins, including Rab10, at a specific residue located at the centre of its effector-binding switch-II...

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Articles

Regulating protein breakdown through proteasome phosphorylation

Jordan J.S. VerPlank, Alfred L. Goldberg

Biochem J (2017) 474 (19): 3355–3371.

DOI: https://doi.org/10.1042/BCJ20160809

Published: 25 September 2017

... that protein half-lives can also be regulated by post-translational modifications of the 26S proteasome. The present study reviews the ability of several kinases to alter proteasome function through subunit phosphorylation. For example, PKA (protein kinase A) and DYRK2 (dual-specificity tyrosine-regulated...

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Articles

Cyclin Y phosphorylation- and 14-3-3-binding-dependent activation of PCTAIRE-1/CDK16

Saifeldin N. Shehata, Maria Deak, Nicholas A. Morrice, Eriko Ohta, Roger W. Hunter, Vera M. Kalscheuer, Kei Sakamoto

Biochem J (2015) 469 (3): 409–420.

DOI: https://doi.org/10.1042/BJ20150486

Published: 23 July 2015

... This is an open access article published by Portland Press Limited and distributed under the Creative Commons Attribution License 3.0 . 14-3-3 cyclin-dependent kinase (CDK) intellectual disability mass spectrometry protein kinase We have identified a key molecular mechanism by which cyclin Y...

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Articles

A protein kinase screen of Neurospora crassa mutant strains reveals that the SNF1 protein kinase promotes glycogen synthase phosphorylation

Thiago De Souza Candido, Rodrigo Duarte Gonçalves, Ana Paula Felício, Fernanda Zanolli Freitas, Fernanda Barbosa Cupertino, Ana Carolina Gomes Vieira De Carvalho, Maria Célia Bertolini

Biochem J (2014) 464 (3): 323–334.

DOI: https://doi.org/10.1042/BJ20140942

Published: 05 December 2014

.... The activities of glycogen synthase and glycogen phosphorylase, the rate-limiting enzymes of the synthesis and degradation processes, respectively, are regulated by allosteric modulation and reversible phosphorylation. To identify the protein kinases affecting glycogen metabolism in Neurospora crassa , we...

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Articles

Adenylate cyclase-centred microdomains

Dermot M. F. Cooper, Valentina G. Tabbasum

Biochem J (2014) 462 (2): 199–213.

DOI: https://doi.org/10.1042/BJ20140560

Published: 07 August 2014

..., the large number of cellular processes modulated by only three intermediaries, i.e. PKA (protein kinase A), Epacs (exchange proteins directly activated by cAMP) and CNG (cyclic nucleotide-gated) channels, poses the question of how selectivity and fine control is achieved by cAMP. One answer rests...

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Articles

Kinase and channel activity of TRPM6 are co-ordinated by a dimerization motif and pocket interaction

Jenny van der Wijst, Maxime G. Blanchard, Helen I. Woodroof, Thomas J. Macartney, Robert Gourlay, Joost G. Hoenderop, René J. Bindels, Dario R. Alessi

Biochem J (2014) 460 (2): 165–175.

DOI: https://doi.org/10.1042/BJ20131639

Published: 13 May 2014

... with secondary hypocalcaemia), a disorder characterized by defective intestinal Mg 2+ transport and impaired renal Mg 2+ reabsorption. TRPM6, together with its homologue TRPM7, are unique proteins as they combine an ion channel domain with a C-terminally fused protein kinase domain. How TRPM6 channel and kinase...

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Articles

The WNK-regulated SPAK/OSR1 kinases directly phosphorylate and inhibit the K + –Cl − co-transporters

Paola de los Heros, Dario R. Alessi, Robert Gourlay, David G. Campbell, Maria Deak, Thomas J. Macartney, Kristopher T. Kahle, Jinwei Zhang

Biochem J (2014) 458 (3): 559–573.

DOI: https://doi.org/10.1042/BJ20131478

Published: 28 February 2014

... that the WNK (WNK lysine-deficient protein kinase)-activated SPAK (SPS1-related proline/alanine-rich kinase)/OSR1 (oxidative stress-responsive kinase 1) known to directly phosphorylate and stimulate the N[K]CCs (Na + –K + ion co-transporters), also promote inhibition of the KCCs (K + –Cl − co-transporters...

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Articles

Four-and-a-half LIM domains proteins are novel regulators of the protein kinase D pathway in cardiac myocytes

Konstantina Stathopoulou, Friederike Cuello, Alexandra J. Candasamy, Elizabeth M. Kemp, Elisabeth Ehler, Robert S. Haworth, Metin Avkiran

Biochem J (2014) 457 (3): 451–461.

DOI: https://doi.org/10.1042/BJ20131026

Published: 10 January 2014

...Konstantina Stathopoulou; Friederike Cuello; Alexandra J. Candasamy; Elizabeth M. Kemp; Elisabeth Ehler; Robert S. Haworth; Metin Avkiran PKD (protein kinase D) is a serine/threonine kinase implicated in multiple cardiac roles, including the phosphorylation of the class II HDAC5 (histone...

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Articles

A robust methodology to subclassify pseudokinases based on their nucleotide-binding properties

Biochem J (2014) 457 (2): 323–334.

DOI: https://doi.org/10.1042/BJ20131174

Published: 20 December 2013

...; Christine L. Gee; Ling Liu; Roger J. Daly; Gerard Manning; Jeffrey J. Babon; Isabelle S. Lucet Protein kinase-like domains that lack conserved residues known to catalyse phosphoryl transfer, termed pseudokinases, have emerged as important signalling domains across all kingdoms of life. Although predicted...

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Articles

AKT-ing out: SGK kinases come to the fore

Larissa S. Moniz, Bart Vanhaesebroeck

Biochem J (2013) 452 (3): e11–e13.

DOI: https://doi.org/10.1042/BJ20130617

Published: 31 May 2013

... and the protein kinases SGK2 and PAK3 Proc. Natl. Acad. Sci. U.S.A. 2010 107 12463 12468 11 Chandarlapaty S. Sawai A. Scaltriti M. Rodrik-Outmezguine V. Grbovic-Huezo O. Serra V. Majumder P. K. Baselga J. Rosen N. AKT inhibition relieves feedback suppression...

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Articles

Characterization of Staphylococcus aureus EssB, an integral membrane component of the Type VII secretion system: atomic resolution crystal structure of the cytoplasmic segment

Martin Zoltner, Paul K. Fyfe, Tracy Palmer, William N. Hunter

Biochem J (2013) 449 (2): 469–477.

DOI: https://doi.org/10.1042/BJ20121209

Published: 14 December 2012

... Å=0.1 nm) resolution. EssB-N is dimeric in solution, but crystallizes as a monomer and displays a fold comprised of two globular domains separated by a cleft. The structure is related to that of serine/threonine protein kinases and the present study identifies that the Type VII secretion system exploits...

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Articles

Postal code for a plant MAPK

Brian E. Ellis

Biochem J (2012) 446 (2): e5–e7.

DOI: https://doi.org/10.1042/BJ20121003

Published: 14 August 2012

...Brian E. Ellis Plants contain hundreds of protein kinases that are believed to provide cellular signal transduction services, but the identities of the proteins they are targeting are largely unknown. Using an Arabidopsis MAPK (mitogen-activated protein kinase) (MPK6) as a model, Sörensson et al...

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Articles

The MEKK1 SWIM domain is a novel substrate receptor for c-Jun ubiquitylation

Michael A. Rieger, Tyler Duellman, Christopher Hooper, Magdalene Ameka, Joanna C. Bakowska, Bruce D. Cuevas

Biochem J (2012) 445 (3): 431–439.

DOI: https://doi.org/10.1042/BJ20120406

Published: 13 July 2012

...Michael A. Rieger; Tyler Duellman; Christopher Hooper; Magdalene Ameka; Joanna C. Bakowska; Bruce D. Cuevas MEKK1 [MAPK (mitogen-activated protein kinase)/ERK (extracellular-signal-regulated kinase) kinase kinase 1] is a MAP3K (MAPK kinase kinase) that regulates MAPK activation, and is the only...

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Articles

Regulation and function of the RSK family of protein kinases

Yves Romeo, Xiaocui Zhang, Philippe P. Roux

Biochem J (2012) 441 (2): 553–569.

DOI: https://doi.org/10.1042/BJ20110289

Published: 21 December 2011

... 2012 1 To whom correspondence should be addressed (email philippe.roux@umontreal.ca ). 14 2 2011 11 8 2011 11 8 2011 cell signalling mitogen-activated protein kinase (MAPK) phosphorylation proliferation protein kinase 90 kDa ribosomal S6 kinase (RSK...

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Articles

SPAK/OSR1 regulate NKCC1 and WNK activity: analysis of WNK isoform interactions and activation by T-loop trans-autophosphorylation

Jacob O. Thastrup, Fatema H. Rafiqi, Alberto C. Vitari, Eulalia Pozo-Guisado, Maria Deak, Youcef Mehellou, Dario R. Alessi

Biochem J (2012) 441 (1): 325–337.

DOI: https://doi.org/10.1042/BJ20111879

Published: 14 December 2011

..., which is activated in response to osmotic stress by phosphorylation of its T-loop residue (Ser 382 ). WNK isoforms phosphorylate and activate the related SPAK (SPS1-related proline/alanine-rich kinase) and OSR1 (oxidative stress-responsive kinase 1) protein kinases. In the present study, we first...

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Articles

TTBK2 kinase substrate specificity and the impact of spinocerebellar-ataxia-causing mutations on expression, activity, localization and development

Michale Bouskila, Noor Esoof, Laurie Gay, Emily H. Fang, Maria Deak, Michael J. Begley, Lewis C. Cantley, Alan Prescott, Kate G. Storey, Dario R. Alessi

Biochem J (2011) 437 (1): 157–167.

DOI: https://doi.org/10.1042/BJ20110276

Published: 14 June 2011

... nuclear localization. We generate an SCA11-mutation-carrying knockin mouse and show that this leads to inhibition of endogenous TTBK2 protein kinase activity. Finally, we find that, in homozygosity, the SCA11 mutation causes embryonic lethality at embryonic day 10. These findings provide the first...

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Articles

Inhibition of the mitochondrial permeability transition by protein kinase A in rat liver mitochondria and hepatocytes

Peter Pediaditakis, Jae-Sung Kim, Lihua He, Xun Zhang, Lee M. Graves, John J. Lemasters

Biochem J (2010) 431 (3): 411–421.

DOI: https://doi.org/10.1042/BJ20091741

Published: 11 October 2010

.... Specific peptide inhibitors for PKA (protein kinase A), but not PKG (protein kinase G), abolished cytosol-induced inhibition of MPT onset. Activity assays showed a cGMP- and cAMP-stimulated protein kinase activity in liver cytosol that was completely inhibited by PKI, a PKA peptide inhibitor. Size...

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Articles

Key motifs in EBV (Epstein–Barr virus)-encoded protein kinase for phosphorylation activity and nuclear localization

Svetlana Gershburg, Leann Murphy, Manfred Marschall, Edward Gershburg

Biochem J (2010) 431 (2): 227–235.

DOI: https://doi.org/10.1042/BJ20100558

Published: 28 September 2010

...Svetlana Gershburg; Leann Murphy; Manfred Marschall; Edward Gershburg A sole EBV (Epstein–Barr virus)-encoded protein kinase (EBV-PK) (the BGLF4 gene product) plays important roles in viral infection. Although a number of targets of this protein have been identified, the kinase itself remains...

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Articles

Guidelines for the effective use of chemical inhibitors of protein function to understand their roles in cell regulation

Philip Cohen

Biochem J (2010) 425 (1): 53–54.

DOI: https://doi.org/10.1042/BJ20091428

Published: 14 December 2009

...Philip Cohen Cell-permeable chemical inhibitors have become invaluable reagents for the study of cellular regulation. In the present paper, using protein kinase inhibitors as the example, a set of criteria are described that should be met before any investigation using such compounds should...

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Articles

The C-terminal domain of Mnk1a plays a dual role in tightly regulating its activity

Susan Goto, Zhong Yao, Christopher G. Proud

Biochem J (2009) 423 (2): 279–290.

DOI: https://doi.org/10.1042/BJ20090228

Published: 25 September 2009

...Susan Goto; Zhong Yao; Christopher G. Proud The human family of MAPK (mitogen-activated protein kinase) signal-integrating kinases (Mnks) comprises four related proteins derived from two genes by alternative splicing. The MNK1 gene gives rise to two proteins, Mnk1a and Mnk1b, which possess distinct...

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Articles

PI3Kγ controls oxidative bursts in neutrophils via interactions with PKCα and p47phox

Katja Lehmann, Jörg P. Müller, Bernhard Schlott, Philipp Skroblin, Dagmar Barz, Johannes Norgauer, Reinhard Wetzker

Biochem J (2009) 419 (3): 603–610.

DOI: https://doi.org/10.1042/BJ20081268

Published: 14 April 2009

... the association of PI3Kγ with protein kinase Cα (PKCα). Specific inhibition of PI3Kγ suppresses fMLP-mediated activation of PKCα activity and ROS production, suggesting that the protein kinase activity of PI3Kγ is involved. Our data suggest that the direct interaction of PI3Kγ with PKCα forms a discrete...

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Articles

Snf1-related protein kinases (SnRKs) act within an intricate network that links metabolic and stress signalling in plants

Nigel G. Halford, Sandra J. Hey

Biochem J (2009) 419 (2): 247–259.

DOI: https://doi.org/10.1042/BJ20082408

Published: 27 March 2009

...Nigel G. Halford; Sandra J. Hey The phosphorylation and dephosphorylation of proteins, catalysed by protein kinases and phosphatases, is the major mechanism for the transduction of intracellular signals in eukaryotic organisms. Signalling pathways often comprise multiple phosphorylation...

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Articles

The hydrophobic motif of ROCK2 requires association with the N-terminal extension for kinase activity

Amber L. Couzens, Vivian Saridakis, Michael P. Scheid

Biochem J (2009) 419 (1): 141–148.

DOI: https://doi.org/10.1042/BJ20081376

Published: 13 March 2009

... 12 2008 © The Authors Journal compilation © 2009 Biochemical Society 2009 cytoskeleton protein kinase Rho Rho-associated coiled-coil kinase (ROCK) signal transduction ROCK (Rho-associated coiled-coil kinase) [also called ROK (Rho-associated kinase) and Rho kinase] is a large...

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Articles

Activation of a nuclear-localized SIPK in tobacco cells challenged by cryptogein, an elicitor of plant defence reactions

Jennifer Dahan, Carole Pichereaux, Michel Rossignol, Sabrina Blanc, David Wendehenne, Alain Pugin, Stéphane Bourque

Biochem J (2009) 418 (1): 191–200.

DOI: https://doi.org/10.1042/BJ20081465

Published: 28 January 2009

... to develop adaptive responses. In this context, protein phosphorylation plays a fundamental role through the activation of multiple protein kinase families. Although the involvement of protein kinases at the plasma membrane and cytosolic levels are now well-documented, their nuclear counterparts are still...

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Articles

Translational regulation of lipoprotein lipase in adipocytes: depletion of cellular protein kinase Cα activates binding of the C subunit of protein kinase A to the 3′-untranslated region of the lipoprotein lipase mRNA

Resat Unal, Irina Pokrovskaya, Preeti Tripathi, Brett P. Monia, Philip A. Kern, Gouri Ranganathan

Biochem J (2008) 413 (2): 315–322.

DOI: https://doi.org/10.1042/BJ20071559

Published: 26 June 2008

...Resat Unal; Irina Pokrovskaya; Preeti Tripathi; Brett P. Monia; Philip A. Kern; Gouri Ranganathan Adipose LPL (lipoprotein lipase) plays an important role in regulating plasma triacylglycerols and lipid metabolism. We have previously demonstrated that PKCα (protein kinase Cα) depletion inhibits LPL...

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Articles

Phosphorylation of eIF2α in response to 26S proteasome inhibition is mediated by the haem-regulated inhibitor (HRI) kinase

Azmi Yerlikaya, Scot R. Kimball, Bruce A. Stanley

Biochem J (2008) 412 (3): 579–588.

DOI: https://doi.org/10.1042/BJ20080324

Published: 28 May 2008

... lacking one of the four kinases known to phosphorylate eIF2α, increased phosphorylation of eIF2α still occurred after inhibition of the 26S proteasome. These three cell lines included a deletion of the PKR (double-stranded-RNA-dependent protein kinase); a deletion of the PERK (PKR-like endoplasmic...

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Articles

AMPK, MAPK and Bax in the heart: some questions answered

Vilmante Borutaite

Biochem J (2008) 412 (2): e15–e16.

DOI: https://doi.org/10.1042/BJ20080712

Published: 14 May 2008

... presented their discovery that simulated ischaemia causes rapid activation of AMPK (AMP-activated protein kinase) which phosphorylates and activates p38 MAPK (mitogen-activated protein kinase) leading to Bax activation and translocation to mitochondria in isolated cardiac myocytes. This was the first report...

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Articles

Control of AMPK-related kinases by USP9X and atypical Lys 29 /Lys 33 -linked polyubiquitin chains

Abdallah K. Al-Hakim, Anna Zagorska, Louise Chapman, Maria Deak, Mark Peggie, Dario R. Alessi

Biochem J (2008) 411 (2): 249–260.

DOI: https://doi.org/10.1042/BJ20080067

Published: 27 March 2008

...Abdallah K. Al-Hakim; Anna Zagorska; Louise Chapman; Maria Deak; Mark Peggie; Dario R. Alessi AMPK (AMP-activated protein kinase)-related kinases regulate cell polarity as well as proliferation and are activated by the LKB1-tumour suppressor kinase. In the present study we demonstrate that the AMPK...

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Articles

Plk3 phosphorylates topoisomerase IIα at Thr 1342 , a site that is not recognized by Plk1

Masato Iida, Masao Matsuda, Hideya Komatani

Biochem J (2008) 411 (1): 27–32.

DOI: https://doi.org/10.1042/BJ20071394

Published: 13 March 2008

... (email hideya_komatani@merck.com ). 10 10 2007 5 12 2007 7 12 2007 7 12 2007 © The Authors Journal compilation © 2008 Biochemical Society 2008 consensus sequence peptide substrate Plk3 protein kinase topoisomerase IIα substrate specificity Plk (polo-like...

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Articles

Characterization of a new family of protein kinases from Arabidopsis containing phosphoinositide 3/4-kinase and ubiquitin-like domains

Rafaelo M. Galvão, Uma Kota, Erik J. Soderblom, Michael B. Goshe, Wendy F. Boss

Biochem J (2008) 409 (1): 117–127.

DOI: https://doi.org/10.1042/BJ20070959

Published: 11 December 2007

... of the present results, we propose that AtPI4Kγ4 and AtPI4Kγ7 should be designated UbDKγ4 and UbDKγ7 ( ub iquitin-like d omain k inases γ4 and γ7). These UBL-domain-containing AtPI4Ks correspond to a new PIKK subfamily of protein kinases. Furthermore, UFD1 and RPN10 phosphorylation represents an additional...

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Articles

The selectivity of protein kinase inhibitors: a further update

Jenny Bain, Lorna Plater, Matt Elliott, Natalia Shpiro, C. James Hastie, Hilary Mclauchlan, Iva Klevernic, J. Simon C. Arthur, Dario R. Alessi, Philip Cohen

Biochem J (2007) 408 (3): 297–315.

DOI: https://doi.org/10.1042/BJ20070797

Published: 28 November 2007

...Jenny Bain; Lorna Plater; Matt Elliott; Natalia Shpiro; C. James Hastie; Hilary Mclauchlan; Iva Klevernic; J. Simon C. Arthur; Dario R. Alessi; Philip Cohen The specificities of 65 compounds reported to be relatively specific inhibitors of protein kinases have been profiled against a panel of 70–80...

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Articles

Identification of Protor as a novel Rictor-binding component of mTOR complex-2

Laura R. Pearce, Xu Huang, Jérôme Boudeau, Rafał Pawłowski, Stephan Wullschleger, Maria Deak, Adel F. M. Ibrahim, Robert Gourlay, Mark A. Magnuson, Dario R. Alessi

Biochem J (2007) 405 (3): 513–522.

DOI: https://doi.org/10.1042/BJ20070540

Published: 13 July 2007

...Laura R. Pearce; Xu Huang; Jérôme Boudeau; Rafał Pawłowski; Stephan Wullschleger; Maria Deak; Adel F. M. Ibrahim; Robert Gourlay; Mark A. Magnuson; Dario R. Alessi The mTOR (mammalian target of rapamycin) protein kinase is an important regulator of cell growth. Two complexes of mTOR have been...

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LRRK2 phosphorylates moesin at threonine-558: characterization of how Parkinson's disease mutants affect kinase activity

Mahaboobi Jaleel, R. Jeremy Nichols, Maria Deak, David G. Campbell, Frank Gillardon, Axel Knebel, Dario R. Alessi

Biochem J (2007) 405 (2): 307–317.

DOI: https://doi.org/10.1042/BJ20070209

Published: 27 June 2007

... 20 4 2007 20 4 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 ezrin/radixin/moesin family of proteins (ERM proteins) kinase substrate tracking and elucidation screening (KESTREL screening) leucine-rich repeat kinase 2 (LRRK2) mass spectrometry (MS...

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Dsk1p kinase phosphorylates SR proteins and regulates their cellular localization in fission yeast

Zhaohua Tang, Amy Tsurumi, Sarah Alaei, Christopher Wilson, Cathleen Chiu, Jessica Oya, Benson Ngo

Biochem J (2007) 405 (1): 21–30.

DOI: https://doi.org/10.1042/BJ20061523

Published: 13 June 2007

... previously identified Dsk1p ( dis1 -suppressing protein kinase) as the orthologue of human SRPK1 in fission yeast. In addition to its similarity of gene structure to higher eukaryotes, fission yeast Schizosaccharomyces pombe is a unicellular eukaryotic organism in which alternative splicing takes place...

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Articles

Functional interactions of the SPAK/OSR1 kinases with their upstream activator WNK1 and downstream substrate NKCC1

Alberto C. Vitari, Jacob Thastrup, Fatema H. Rafiqi, Maria Deak, Nick A. Morrice, Håkan K. R. Karlsson, Dario R. Alessi

Biochem J (2006) 397 (1): 223–231.

DOI: https://doi.org/10.1042/BJ20060220

Published: 14 June 2006

...-1), leading to its activation. Recent studies indicated that SPAK and OSR1 are phosphorylated and activated by the WNK1 [with no K (lysine) protein kinase-1] and WNK4, genes mutated in humans affected by Gordon's hypertension syndrome. In the present study, we have identified three residues in NKCC1...

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Articles

A Pak- and Pix-dependent branch of the SDF-1α signalling pathway mediates T cell chemotaxis across restrictive barriers

Natalia Volinsky, Anna Gantman, Deborah Yablonski

Biochem J (2006) 397 (1): 213–222.

DOI: https://doi.org/10.1042/BJ20051655

Published: 14 June 2006

... The Biochemical Society, London 2006 chemotaxis enzyme inhibitor p21-activated kinase (Pak) protein kinase signal transduction T cell Chemokines, such as SDF-1α (stromal-cell derived factor-1α/CXCL12), trigger profound, spatially polarized cytoskeletal changes [ 1 , 2 ] that culminate...

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Recombinant human hyaluronan synthase 3 is phosphorylated in mammalian cells

Brian J. Goentzel, Paul H. Weigel, Robert A. Steinberg

Biochem J (2006) 396 (2): 347–354.

DOI: https://doi.org/10.1042/BJ20051782

Published: 15 May 2006

... the mammalian HAS proteins, no previous studies have directly investigated possible control of the HAS enzymes by post-translational modification. Nevertheless, there is indirect evidence for regulation of HAS activity by PKC (protein kinase C), PKA (protein kinase A) and/or by a calcium-dependent protein...

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Articles

The WNK1 and WNK4 protein kinases that are mutated in Gordon's hypertension syndrome phosphorylate and activate SPAK and OSR1 protein kinases

Alberto C. Vitari, Maria Deak, Nick A. Morrice, Dario R. Alessi

Biochem J (2005) 391 (1): 17–24.

DOI: https://doi.org/10.1042/BJ20051180

Published: 26 September 2005

...Alberto C. Vitari; Maria Deak; Nick A. Morrice; Dario R. Alessi Mutations in the human genes encoding WNK1 [with no K (lysine) protein kinase-1] and the related protein kinase WNK4 are the cause of Gordon's hypertension syndrome. Little is known about the molecular mechanism by which WNK isoforms...

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Aurora-A site specificity: a study with synthetic peptide substrates

Stefano Ferrari, Oriano Marin, Mario A. Pagano, Flavio Meggio, Daniel Hess, Mahmoud El-Shemerly, Agnieszka Krystyniak, Lorenzo A. Pinna

Biochem J (2005) 390 (1): 293–302.

DOI: https://doi.org/10.1042/BJ20050343

Published: 09 August 2005

...Stefano Ferrari; Oriano Marin; Mario A. Pagano; Flavio Meggio; Daniel Hess; Mahmoud El-Shemerly; Agnieszka Krystyniak; Lorenzo A. Pinna AurA (Aurora-A) is a ubiquitous protein kinase regulating entry into mitosis and shown to promote transformation upon overexpression. In order to gain information...

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Articles

The Ins(1,3,4) P 3 5/6-kinase/Ins(3,4,5,6) P 4 1-kinase is not a protein kinase

Xun Qian, Jennifer Mitchell, Sung-Jen Wei, Jason Williams, Robert M. Petrovich, Stephen B. Shears

Biochem J (2005) 389 (2): 389–395.

DOI: https://doi.org/10.1042/BJ20050297

Published: 05 July 2005

...(3,4,5,6) P 4 1-kinase in Sf9 insect cells and purified the enzyme using Ni–agarose chromatography. Protein kinase activity was eluted from the Ni–agarose column, but this did not co-elute with the Ins(3,4,5,6) P 4 1-kinase, indicating that the protein kinase and inositol kinase activities belong...

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Articles

DNA-dependent phosphorylation of Chk1 and Claspin in a human cell-free system

Catriona A. L. CLARKE, Paul R. CLARKE

Biochem J (2005) 388 (2): 705–712.

DOI: https://doi.org/10.1042/BJ20041966

Published: 24 May 2005

... Xenopus eggs. In the present study, we describe a human cell-free system that reproduces a DNA-dependent checkpoint pathway acting on the Chk1 protein kinase. In this system, double-stranded DNA oligonucleotides induce the phosphorylation of Chk1 at activating sites targeted by ATR [ATM (ataxia...

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Articles

C-terminal splicing of NTPDase2 provides distinctive catalytic properties, cellular distribution and enzyme regulation

Carol J. H. WANG, Srdjan M. VLAJKOVIC, Gary D. HOUSLEY, Norbert BRAUN, Herbert ZIMMERMANN, Simon C. ROBSON, Jean SÉVIGNY, Christian SOELLER, Peter R. THORNE

Biochem J (2005) 385 (3): 729–736.

DOI: https://doi.org/10.1042/BJ20040852

Published: 24 January 2005

... intracellular protein kinase CK2 (casein kinase 2) phosphorylation sites and losing the intracellular protein kinase C motif. The plasmids containing NTPDase2α or NTPDase2β cDNA were used to stably transfect Chinese-hamster ovary-S cells. Imaging studies showed that NTPDase2α was predominantly membrane-bound...

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Articles

The Drosophila protein kinase LK6 is regulated by ERK and phosphorylates the eukaryotic initiation factor eIF4E in vivo

Josep L. PARRA-PALAU, Gert C. SCHEPER, Daniel E. HARPER, Christopher G. PROUD

Biochem J (2005) 385 (3): 695–702.

DOI: https://doi.org/10.1042/BJ20040769

Published: 24 January 2005

...-activated protein kinase) signal-integrating kinases 1 and 2 respectively] that phosphorylate mammalian eIF4E. Mnk1 is activated by both mitogen- and stress-activated signalling pathways [ERK (extracellular-signal-regulated kinase) and p38 MAPK], whereas Mnk2 contains a MAPK-binding motif that is selective...

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Kinetic analysis of ligand-induced autocatalytic reactions

Jiang-Hong LIU, Zhi-Xin WANG

Biochem J (2004) 379 (3): 697–702.

DOI: https://doi.org/10.1042/bj20031365

Published: 01 May 2004

...Jiang-Hong LIU; Zhi-Xin WANG Protein phosphorylation and limited proteolysis are two most common regulatory mechanisms involving the energy-dependent covalent modification of regulatory enzymes. In addition to modifying other proteins, many protein kinases and proteases catalyse automodification...

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Analysis of the interaction between piD261/Bud32, an evolutionarily conserved protein kinase of Saccharomyces cerevisiae, and the Grx4 glutaredoxin

Raffaele LOPREIATO, Sonia FACCHIN, Geppo SARTORI, Giorgio ARRIGONI, Stefano CASONATO, Maria RUZZENE, Lorenzo A. PINNA, Giovanna CARIGNANI

Biochem J (2004) 377 (2): 395–405.

DOI: https://doi.org/10.1042/bj20030638

Published: 15 January 2004

... kinase family (the piD261 family) distantly related in sequence to the eukaryotic protein kinase superfamily. It has been demonstrated that the yeast protein displays Ser/Thr phosphotransferase activity in vitro and contains all the invariant residues of the family. This novel protein kinase appears...

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Articles

Distinct regulation of cytosolic phospholipase A2 phosphorylation, translocation, proteolysis and activation by tumour necrosis factor-receptor subtypes

Orla J. JUPP, Peter VANDENABEELE, David J. MacEWAN

Biochem J (2003) 374 (2): 453–461.

DOI: https://doi.org/10.1042/bj20030705

Published: 01 September 2003

... TNF receptor subtypes are involved in activating the lipase. In the present study, we show for the first time that both type I 55 kDa TNFR (TNFR1) and type II 75 kDa TNFR (TNFR2) stimulate cPLA 2 enzyme, but with distinct signalling mechanisms. TNFR1 activates mitogen-activated protein kinase (MAPK...

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The role of two novel regulatory sites in the activation of the cGMP-dependent protein kinase from Plasmodium falciparum

Wensheng DENG, Asha PARBHU-PATEL, David J. MEYER, David A. BAKER

Biochem J (2003) 374 (2): 559–565.

DOI: https://doi.org/10.1042/bj20030474

Published: 01 September 2003

...Wensheng DENG; Asha PARBHU-PATEL; David J. MEYER; David A. BAKER The Plasmodium falciparum cGMP-dependent protein kinase (PfPKG) uniquely contains three cGMP binding sites, but also has a ‘degenerate’ fourth site. The role of each cGMP-binding site in PfPKG activation remains unknown. We have...

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