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Articles

Monoclonal antibody stability can be usefully monitored using the excitation-energy-dependent fluorescence edge-shift

Michael J. Knight, Rachel E. Woolley, Anthony Kwok, Stuart Parsons, Hannah B. L. Jones, Christina E. Gulácsy, Polly Phaal, Omar Kassaar, Kieran Dawkins, Elizabeth Rodriguez, Andreia Marques, Leo Bowsher, Stephen A. Wells, Andrew Watts, Jean M. H. van den Elsen, Alison Turner, John O'Hara, Christopher R. Pudney

Biochem J (2020) 477 (18): 3599–3612.

https://doi.org/10.1042/BCJ20200580

Published: 28 September 2020

... and the Biochemical Society under a transformative agreement with JISC. antibodies edge-shift fluorescence spectroscopy protein dynamics protein stability Maintenance of function and hence efficacy is an important consideration for the developability of biomolecules. This is driven in part...

View articletitled, Monoclonal antibody stability can be usefully monitored using the excitation-energy-dependent fluorescence edge-shift

Includes: Supplementary data

Articles

Lysosomal protein thermal stability does not correlate with cellular half-life: global observations and a case study of tripeptidyl-peptidase 1

Aaron M. Collier, Yuliya Nemtsova, Narendra Kuber, Whitney Banach-Petrosky, Anurag Modak, David E. Sleat, Vikas Nanda, Peter Lobel

Biochem J (2020) 477 (3): 727–745.

https://doi.org/10.1042/BCJ20190874

Published: 14 February 2020

...). Published by Portland Press Limited on behalf of the Biochemical Society 2020 enzyme replacement therapy lysosomal storage diseases neuronal ceroid lipofuscinoses protein engineering protein stability tripeptidases TPP1 is a member of the sedolisin (MEROPS S53) family [ 6 ]. These unusual...

View articletitled, Lysosomal protein thermal stability does not correlate with cellular half-life: global observations and a case study of tripeptidyl-peptidase 1

Includes: Supplementary data

Articles

The chromatin nuclear protein NUPR1L is intrinsically disordered and binds to the same proteins as its paralogue

José L. Neira, María Belén López, Paz Sevilla, Bruno Rizzuti, Ana Cámara-Artigas, Miguel Vidal, Juan L. Iovanna

Biochem J (2018) 475 (14): 2271–2291.

https://doi.org/10.1042/BCJ20180365

Published: 25 July 2018

... binding IDP molecular interactions nuclear–protein protein conformation protein stability NUPR1 is an 82-residue-long (8 kDa), highly basic, monomeric IDP that is overexpressed during the acute phase of pancreatitis [ 1 , 2 ]. It does not have a stable secondary and tertiary structure in any...

View articletitled, The chromatin nuclear protein NUPR1L is intrinsically disordered and binds to the same proteins as its paralogue

Includes: Supplementary data

Articles

Trade-offs with stability modulate innate and mutationally acquired drug resistance in bacterial dihydrofolate reductase enzymes

Nishad Matange, Swapnil Bodkhe, Maitri Patel, Pooja Shah

Biochem J (2018) 475 (12): 2107–2125.

https://doi.org/10.1042/BCJ20180249

Published: 29 June 2018

... molecular evolution protein stability trade-off trimethoprim During the evolution of proteins, structural stability constrains the sequence space that a protein can access and mutations that have large destabilizing effects are usually eliminated by purifying selection. At the same time...

View articletitled, Trade-offs with stability modulate innate and mutationally acquired drug resistance in bacterial dihydrofolate reductase enzymes

Includes: Supplementary data

Articles

Role of glycosylation in nucleating protein folding and stability

Nisha Grandhi Jayaprakash, Avadhesha Surolia

Biochem J (2017) 474 (14): 2333–2347.

https://doi.org/10.1042/BCJ20170111

Published: 03 July 2017

... of the Biochemical Society 2017 glycosylation protein function protein stability protein structure Glycosylation is the covalent addition of sugar moieties to the protein co-translationally or as a post-translational modification enzymatically. It is the most frequent, heterogeneous, stereo...

View articletitled, Role of glycosylation in nucleating protein folding and stability

Articles

The G2385R risk factor for Parkinson's disease enhances CHIP-dependent intracellular degradation of LRRK2

Iakov N. Rudenko, Alice Kaganovich, Rebekah G. Langston, Aleksandra Beilina, Kelechi Ndukwe, Ravindran Kumaran, Allissa A. Dillman, Ruth Chia, Mark R. Cookson

Biochem J (2017) 474 (9): 1547–1558.

https://doi.org/10.1042/BCJ20160909

Published: 24 April 2017

... a penetrant variant for inherited PD. CHIP G-proteins Parkinson's disease proteasome protein stability † Present address: Medical College of Wisconsin, Medical School, 8701 W Watertown Plank Rd, Milwaukee, WI 53226, U.S.A. ‡ Present address: Center for Cancer Research, Laboratory...

View articletitled, The G2385R risk factor for Parkinson's disease enhances CHIP-dependent intracellular degradation of LRRK2

Includes: Supplementary data

Articles

Functional consequences of B-repeat sequence variation in the staphylococcal biofilm protein Aap: deciphering the assembly code

Catherine L. Shelton, Deborah G. Conrady, Andrew B. Herr

Biochem J (2017) 474 (3): 427–443.

https://doi.org/10.1042/BCJ20160675

Published: 20 January 2017

... a significant reduction in protein stability, equivalent to loss of the entire downstream G5 domain [ 21 ]. As predicted by the biophysical studies, dimeric Brpt1.5 crystallized in the presence of Zn 2+ with two antiparallel protein chains co-ordinating the Zn 2+ in trans and forming dimer interfaces via...

View articletitled, Functional consequences of B-repeat sequence variation in the staphylococcal biofilm protein Aap: deciphering the assembly code

Includes: Supplementary data

Articles

Engineering ancestral protein hyperstability

M. Luisa Romero-Romero, Valeria A. Risso, Sergio Martinez-Rodriguez, Beatriz Ibarra-Molero, Jose M. Sanchez-Ruiz

Biochem J (2016) 473 (20): 3611–3620.

https://doi.org/10.1042/BCJ20160532

Published: 11 October 2016

... Magliery , T.J. ( 2015 ) Protein stability: computation, sequence statistics, and new experimental methods . Curr. Opin. Struct. Biol. 33 , 161 – 168 doi: 10.1016/j.sbi.2015.09.002 3 Bloom , J.D. , Labthavikul , S.T. , Otey , C.R. and Arnold , F.H. ( 2006 ) Protein...

View articletitled, Engineering ancestral protein hyperstability

Articles

Congenital microcornea-cataract syndrome-causing mutation X253R increases βB1-crystallin hydrophobicity to promote aggregate formation

Xiao-Yao Leng, Hai-Yun Li, Jing Wang, Liang-Bo Qi, Yi-Bo Xi, Yong-Bin Yan

Biochem J (2016) 473 (14): 2087–2096.

https://doi.org/10.1042/BCJ20160247

Published: 12 July 2016

... protein stability Crystallins are the predominant soluble proteins in vertebrate lens. According to their elution positions on size-exclusion chromatography (SEC), crystallins can be categorized into three families (α-, β- and γ-crystallins) with distinct molecular sizes [ 1 ]. Among them, α...

View articletitled, Congenital microcornea-cataract syndrome-causing mutation X253R increases βB1-crystallin hydrophobicity to promote aggregate formation

Includes: Supplementary data

Articles

An antibody raised against a pathogenic serpin variant induces mutant-like behaviour in the wild-type protein

James A. Irving, Elena Miranda, Imran Haq, Juan Perez, Vadim R. Kotov, Sarah V. Faull, Neda Motamedi-Shad, David A. Lomas

Biochem J (2015) 468 (1): 99–108.

https://doi.org/10.1042/BJ20141569

Published: 05 May 2015

..., expresses a cryptic epitope also present in the oligomer. aggregation antibody α 1 -antitrypsin polymerization protein stability α 1 -Antitrypsin ( SERPINA1 ) is an acute-phase glycoprotein, secreted by hepatocytes and abundant in the plasma. A member of the serpin superfamily, the primary...

View articletitled, An antibody raised against a pathogenic serpin variant induces mutant-like behaviour in the wild-type protein

Articles

Arc is a flexible modular protein capable of reversible self-oligomerization

Craig Myrum, Anne Baumann, Helene J. Bustad, Marte Innselset Flydal, Vincent Mariaule, Sara Alvira, Jorge Cuéllar, Jan Haavik, Jonathan Soulé, José Maria Valpuesta, José Antonio Márquez, Aurora Martinez, Clive R. Bramham

Biochem J (2015) 468 (1): 145–158.

https://doi.org/10.1042/BJ20141446

Published: 05 May 2015

..., is monomeric but also forms oligomers, which posits Arc to be a flexible hub protein. Arc biophysical characterization oligomerization presenilin-1 protein purification protein stability synaptic plasticity Storage of information in the brain is thought to require the plasticity of neural...

View articletitled, Arc is a flexible modular protein capable of reversible self-oligomerization

Includes: Supplementary data

Articles

Protein stability versus function: effects of destabilizing missense mutations on BRCA1 DNA repair activity

David C.A. Gaboriau, Pamela J.E. Rowling, Ciaran G. Morrison, Laura S. Itzhaki

Biochem J (2015) 466 (3): 613–624.

https://doi.org/10.1042/BJ20141077

Published: 06 March 2015

... repair in vivo , but reduced cellular function only weakly correlated with reduced structural stability. The findings have an impact on assessing cancer susceptibility in patients with BRCA1 mutations. BRCA1 breast cancer DNA repair mutation protein stability tumour suppressor gene...

View articletitled, Protein stability versus function: effects of destabilizing missense mutations on BRCA1 DNA repair activity

Includes: Supplementary data

Articles

The consensus-based approach for gene/enzyme replacement therapies and crystallization strategies: the case of human alanine–glyoxylate aminotransferase

Noel Mesa-Torres, Cristina Yunta, Israel Fabelo-Rosa, Juana María Gonzalez-Rubio, José M. Sánchez-Ruiz, Eduardo Salido, Armando Albert, Angel L. Pey

Biochem J (2014) 462 (3): 453–463.

https://doi.org/10.1042/BJ20140250

Published: 22 August 2014

...Noel Mesa-Torres; Cristina Yunta; Israel Fabelo-Rosa; Juana María Gonzalez-Rubio; José M. Sánchez-Ruiz; Eduardo Salido; Armando Albert; Angel L. Pey Protein stability is a fundamental issue in biomedical and biotechnological applications of proteins. Among these applications, gene- and enzyme...

View articletitled, The consensus-based approach for gene/enzyme replacement therapies and crystallization strategies: the case of human alanine–glyoxylate aminotransferase

Articles

Spinocerebellar ataxia-13 K v 3.3 potassium channels: arginine-to-histidine mutations affect both functional and protein expression on the cell surface

Jian Zhao, Jing Zhu, William B. Thornhill

Biochem J (2013) 454 (2): 259–265.

https://doi.org/10.1042/BJ20130034

Published: 09 August 2013

... (spinocerebellar ataxia type 13). 1 To whom correspondence should be addressed (email [email protected] ). 4 1 2013 22 5 2013 5 6 2013 5 6 2013 © The Authors Journal compilation © 2013 Biochemical Society 2013 potassium channel protein expression protein stability...

View articletitled, Spinocerebellar ataxia-13 K v 3.3 potassium channels: arginine-to-histidine mutations affect both functional and protein expression on the cell surface

Articles

The folding capacity of the mature domain of the dual-targeted plant tRNA nucleotidyltransferase influences organelle selection

Matthew Leibovitch, Daniela Bublak, Pamela J. Hanic-Joyce, Bodo Tillmann, Nadine Flinner, Daniel Amsel, Klaus-Dieter Scharf, Oliver Mirus, Paul B. M. Joyce, Enrico Schleiff

Biochem J (2013) 453 (3): 401–412.

https://doi.org/10.1042/BJ20121577

Published: 12 July 2013

... of four regions, the N-terminal head domain, the neck domain, the body region and the C-terminal tail ( Figure 1 A) [ 5 ]. © The Authors Journal compilation © 2013 Biochemical Society 2013 Arabidopsis thaliana in vivo protein translocation protein stability protein targeting tRNA...

View articletitled, The folding capacity of the mature domain of the dual-targeted plant tRNA nucleotidyltransferase influences organelle selection

Includes: Supplementary data

Articles

DBC1 (Deleted in Breast Cancer 1) modulates the stability and function of the nuclear receptor Rev-erbα

Claudia C. S. Chini, Carlos Escande, Veronica Nin, Eduardo N. Chini

Biochem J (2013) 451 (3): 453–461.

https://doi.org/10.1042/BJ20121085

Published: 12 April 2013

.... In contrast, DBC1 overexpression significantly enhanced Rev-erbα protein stability by preventing its ubiquitination and degradation. The regulation of Rev-erbα protein levels and function by DBC1 depends on both the N-terminal and C-terminal domains of DBC1. More importantly, in cells depleted of DBC1...

View articletitled, DBC1 (Deleted in Breast Cancer 1) modulates the stability and function of the nuclear receptor Rev-erbα

Includes: Supplementary data

Articles

Hsp90 rescues PTK6 from proteasomal degradation in breast cancer cells

Shin-Ae Kang, Hyun-Soo Cho, Jong Bok Yoon, In Kwon Chung, Seung-Taek Lee

Biochem J (2012) 447 (2): 313–320.

https://doi.org/10.1042/BJ20120803

Published: 26 September 2012

...-terminus of Hsc70 (heat-shock cognate 70)-interacting protein (CHIP) heat-shock protein 90 (Hsp90) proteasomal degradation protein stability PTK6 protein tyrosine kinase 6 (PTK6) tyrosine kinase The molecular mechanisms of PTK6 in tumour progression and metastasis have been unveiled...

View articletitled, Hsp90 rescues PTK6 from proteasomal degradation in breast cancer cells

Includes: Supplementary data

Articles

RCD1–DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana

Julia P. Vainonen, Pinja Jaspers, Michael Wrzaczek, Airi Lamminmäki, Ramesha A. Reddy, Lauri Vaahtera, Mikael Brosché, Jaakko Kangasjärvi

Biochem J (2012) 442 (3): 573–581.

https://doi.org/10.1042/BJ20111739

Published: 24 February 2012

...) has been identified as one of the main regulators of drought and heat responses, and it is regulated through protein stability. In the present paper we describe evidence that the interaction with RCD1 (RADICAL-INDUCED CELL DEATH 1) contributes to the control of DREB2A under a range of conditions...

View articletitled, RCD1–DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana

Includes: Supplementary data

Articles

The full-length Streptococcus pneumoniae major pilin RrgB crystallizes in a fibre-like structure, which presents the D1 isopeptide bond and provides details on the mechanism of pilus polymerization

Lamya El Mortaji, Carlos Contreras-Martel, Monica Moschioni, Ilaria Ferlenghi, Clothilde Manzano, Thierry Vernet, Andrea Dessen, Anne Marie Di Guilmi

Biochem J (2012) 441 (3): 833–843.

https://doi.org/10.1042/BJ20111397

Published: 16 January 2012

... compilation © 2012 Biochemical Society 2012 crystal structure of full-length RrgB isopeptide bonds pneumococcus protein stability RrgB polymerization virulence factor Pili are elongated and flexible appendages associated to the bacterial cell surface and play important roles in host tissue...

View articletitled, The full-length Streptococcus pneumoniae major pilin RrgB crystallizes in a fibre-like structure, which presents the D1 isopeptide bond and provides details on the mechanism of pilus polymerization

Includes: Supplementary data

Articles

A common single nucleotide polymorphism A118G of the μ opioid receptor alters its N-glycosylation and protein stability

Peng Huang, Chongguang Chen, Stephen D. Mague, Julie A. Blendy, Lee-Yuan Liu-Chen

Biochem J (2012) 441 (1): 379–386.

https://doi.org/10.1042/BJ20111050

Published: 14 December 2011

... -hMOPR (~28 h). Thus the A118G SNP reduces MOPR N-glycosylation and protein stability. A118G glycosylation G-protein-coupled receptor (GPCR) μ opioid receptor (MOPR) N-glycan protein stability N-linked glycosylation is a common post-translational modification of 7TMRs. The A118G SNP...

View articletitled, A common single nucleotide polymorphism A118G of the μ opioid receptor alters its N-glycosylation and protein stability

Articles

Phosphorylation of JDP2 on threonine-148 by the c-Jun N-terminal kinase targets it for proteosomal degradation

Keren Weidenfeld-Baranboim, Lilach Koren, Ami Aronheim

Biochem J (2011) 436 (3): 661–669.

https://doi.org/10.1042/BJ20101031

Published: 27 May 2011

... that the phosphorylation of Thr 148 may regulate JDP2 protein stability. We then expressed a phosphomimetic JDP2 mutant in which Thr 148 is mutated to a glutamic acid residue (JDP2 T148E) in the JDP2 −/− cells and compared its expression to overexpressed wild-type JDP2 and JDP2 T148A, as well as to endogenous JDP2...

View articletitled, Phosphorylation of JDP2 on threonine-148 by the c-Jun N-terminal kinase targets it for proteosomal degradation

Includes: Supplementary data

Articles

Role of conservative mutations in protein multi-property adaptation

David Rodriguez-Larrea, Raul Perez-Jimenez, Inmaculada Sanchez-Romero, Asuncion Delgado-Delgado, Julio M. Fernandez, Jose M. Sanchez-Ruiz

Biochem J (2010) 429 (2): 243–249.

https://doi.org/10.1042/BJ20100386

Published: 28 June 2010

.... atomic force microscopy (AFM) mutational effect protein evolution protein stability single-molecule analysis Proteins must undergo adaptive changes during evolution when, for instance, the environment surrounding the organism is altered or when they are recruited for new roles [ 1 , 2...

View articletitled, Role of conservative mutations in protein multi-property adaptation

Includes: Supplementary data

Articles

Activating transcription factor 2 increases transactivation and protein stability of hypoxia-inducible factor 1α in hepatocytes

Jeong Hae Choi, Hyun Kook Cho, Yung Hyun Choi, JaeHun Cheong

Biochem J (2009) 424 (2): 285–296.

https://doi.org/10.1042/BJ20090371

Published: 11 November 2009

... demonstrated that the transcription factor ATF-2 (activating transcription factor 2) regulates hypoxia-induced gene transcription, along with HIF-1α. In the present study, we show that the protein stability of ATF-2 is induced by hypoxia and the hypoxia-mimic CoCl 2 (cobalt chloride), and that ATF-2 induction...

View articletitled, Activating transcription factor 2 increases transactivation and protein stability of hypoxia-inducible factor 1α in hepatocytes

Articles

A survey of proteins encoded by non-synonymous single nucleotide polymorphisms reveals a significant fraction with altered stability and activity

Abdellah Allali-Hassani, Gregory A. Wasney, Irene Chau, Bum Soo Hong, Guillermo Senisterra, Peter Loppnau, Zhen Shi, John Moult, Aled M. Edwards, Cheryl H. Arrowsmith, Hee Won Park, Matthieu Schapira, Masoud Vedadi

Biochem J (2009) 424 (1): 15–26.

https://doi.org/10.1042/BJ20090723

Published: 23 October 2009

... was significantly altered, including several variants of human PKM2 (pyruvate kinase muscle 2). Two PKM2 variants, S437Y and E28K, also exhibited changes in their allosteric regulation compared with the wild-type enzyme. The high proportion of nsSNPs that affect protein stability and function, albeit subtly...

View articletitled, A survey of proteins encoded by non-synonymous single nucleotide polymorphisms reveals a significant fraction with altered stability and activity

Includes: Supplementary data

Articles

The presence of monoglucosylated N196-glycan is important for the structural stability of storage protein, arylphorin

Kyoung-Seok Ryu, Jie-Oh Lee, Taek Hun Kwon, Han-Ho Choi, Hong-Seog Park, Soo Kyung Hwang, Zee-Won Lee, Kyung-Bok Lee, Young Hyun Han, Yun-Seok Choi, Young Ho Jeon, Chaejoon Cheong, Soohyun Kim

Biochem J (2009) 421 (1): 87–96.

https://doi.org/10.1042/BJ20082170

Published: 12 June 2009

...-Hydroxyecdysone is the activated form of ecdysone and has been shown to be critical for the maturation of the arylphorin receptor in vivo and in vitro [ 19 ]. arylphorin ecdysone glycosylation protein stability protein structure X-ray crystallography Glycosylation is an important cellular...

View articletitled, The presence of monoglucosylated N196-glycan is important for the structural stability of storage protein, arylphorin

Includes: Supplementary data

Articles

Identification of lysine residues critical for the transcriptional activity and polyubiquitination of the NF-κB family member RelB

Julia Leidner, Lysann Palkowitsch, Uta Marienfeld, Dietmar Fischer, Ralf Marienfeld

Biochem J (2008) 416 (1): 117–127.

https://doi.org/10.1042/BJ20080432

Published: 28 October 2008

... ). 22 2 2008 7 7 2008 11 7 2008 11 7 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 co-factor gene expression nuclear factor κB (NF-κB) protein stability RelB ubiquitination The transcription factor NF-κB (nuclear factor κB) plays...

View articletitled, Identification of lysine residues critical for the transcriptional activity and polyubiquitination of the NF-κB family member RelB

Includes: Supplementary data

Articles

Regulation of Nedd4-2 self-ubiquitination and stability by a PY motif located within its HECT-domain

M. Christine Bruce, Voula Kanelis, Fatemeh Fouladkou, Anne Debonneville, Olivier Staub, Daniela Rotin

Biochem J (2008) 415 (1): 155–163.

https://doi.org/10.1042/BJ20071708

Published: 12 September 2008

...) domain protein stability self-ubiquitination ubiquitin ligase WW domain Ubiquitination plays a critical role in many cellular processes, primarily regulating protein stability and trafficking/endocytosis [ 1 – 3 ]. The ubiquitination cascade is performed by the sequential action of E1, E2...

View articletitled, Regulation of Nedd4-2 self-ubiquitination and stability by a PY motif located within its HECT-domain

Includes: Supplementary data

Articles

Elimination of competing hydrolysis and coupling side reactions of a cyclodextrin glucanotransferase by directed evolution

Ronan M. Kelly, Hans Leemhuis, Henriëtte J. Rozeboom, Niels van Oosterwijk, Bauke W. Dijkstra, Lubbert Dijkhuizen

Biochem J (2008) 413 (3): 517–525.

https://doi.org/10.1042/BJ20080353

Published: 15 July 2008

... 2008 21 4 2008 21 4 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 amylase cyclodextrin glucanotransferase (CGTase) protein evolution protein stability saccharide side reaction Enzymes are essentially a product of evolution. Continuous...

View articletitled, Elimination of competing hydrolysis and coupling side reactions of a cyclodextrin glucanotransferase by directed evolution

Articles

Catecholaminergic polymorphic ventricular tachycardia-related mutations R33Q and L167H alter calcium sensitivity of human cardiac calsequestrin

Giorgia Valle, Daniela Galla, Alessandra Nori, Silvia G. Priori, Sandor Gyorke, Vincenzo de Filippis, Pompeo Volpe

Biochem J (2008) 413 (2): 291–303.

https://doi.org/10.1042/BJ20080163

Published: 26 June 2008

... or [email protected] ). 21 1 2008 8 4 2008 9 4 2008 9 4 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 calcium binding calsequestrin circular dichroism fluorescence protein stability proteolysis The homogeneity of CASQ2 proteins...

View articletitled, Catecholaminergic polymorphic ventricular tachycardia-related mutations R33Q and L167H alter calcium sensitivity of human cardiac calsequestrin

Articles

The chemistry of copper binding to PrP: is there sufficient evidence to elucidate a role for copper in protein function?

Paul Davies, David R. Brown

Biochem J (2008) 410 (2): 237–244.

https://doi.org/10.1042/BJ20071477

Published: 12 February 2008

... The Authors Journal compilation © 2008 Biochemical Society 2008 binding affinity co-ordination copper octapeptide prion protein (PrP) protein stability PrP (prion protein) is a cell-surface glycoprotein that has been directly implicated in the pathogenesis of a range of neurodegenerative...

View articletitled, The chemistry of copper binding to PrP: is there sufficient evidence to elucidate a role for copper in protein function?

Articles

Regulation of neurogenin stability by ubiquitin-mediated proteolysis

Jonathan M. D. Vosper, Christelle S. Fiore-Heriche, Ian Horan, Kate Wilson, Helen Wise, Anna Philpott

Biochem J (2007) 407 (2): 277–284.

https://doi.org/10.1042/BJ20070064

Published: 25 September 2007

... system. NGN activity has been shown extensively to be controlled at the transcriptional level. However, in addition, recent findings have indicated that the levels of NGN protein may also be regulated. In the present study, we have demonstrated that NGN protein stability was regulated in both Xenopus...

View articletitled, Regulation of neurogenin stability by ubiquitin-mediated proteolysis

Articles

PTEN is destabilized by phosphorylation on Thr 366

Helene Maccario, Nevin M. Perera, Lindsay Davidson, C. Peter Downes, Nick R. Leslie

Biochem J (2007) 405 (3): 439–444.

https://doi.org/10.1042/BJ20061837

Published: 13 July 2007

... phosphorylation on protein stability can be revealed, which, in our experiments, are only fulfilled in the glioma cell type U87MG and T98G. This observed effect did appear very potent, as blocking Thr 366 phosphorylation led to an almost complete block in detectable PTEN turnover ( Figure 4 ). Our results...

View articletitled, PTEN is destabilized by phosphorylation on Thr 366

Includes: Supplementary data

Articles

Autophosphorylation-dependent degradation of Pak1, triggered by the Rho-family GTPase, Chp

Monika Weisz Hubsman, Natalia Volinsky, Edward Manser, Deborah Yablonski, Ami Aronheim

Biochem J (2007) 404 (3): 487–497.

https://doi.org/10.1042/BJ20061696

Published: 29 May 2007

[email protected] or [email protected] ). 13 11 2006 5 3 2007 13 3 2007 13 3 2007 © 2007 Biochemical Society 2007 Cdc42 (cell division cycle 42) homologous protein (Chp) GTPase-binding domain p21-activated kinase (Pak) proteasome protein stability Rho-GTPase...

View articletitled, Autophosphorylation-dependent degradation of Pak1, triggered by the Rho-family GTPase, Chp

Articles

Characterization of different isoforms of the HIF prolyl hydroxylase PHD1 generated by alternative initiation

Ya-Min Tian, David R. Mole, Peter J. Ratcliffe, Jonathan M. Gleadle

Biochem J (2006) 397 (1): 179–186.

https://doi.org/10.1042/BJ20051996

Published: 14 June 2006

... active with similar HIF prolyl hydroxylase activity but that they differ in their responses to oestrogen, cell confluence and proteasomal inhibition. We show that the two PHD1 species are subject to proteolytic regulation but differ markedly in their protein stability. Though each isoform has...

View articletitled, Characterization of different isoforms of the HIF prolyl hydroxylase PHD1 generated by alternative initiation

Articles

Resolving the energy paradox of chaperone/usher-mediated fibre assembly

Anton V. Zavialov, Vladimir M. Tischenko, Laura J. Fooks, Bjørn O. Brandsdal, Johan Åqvist, Vladimir P. Zav'yalov, Sheila Macintyre, Stefan D. Knight

Biochem J (2005) 389 (3): 685–694.

https://doi.org/10.1042/BJ20050426

Published: 26 July 2005

... capacity function were calculated from the calorimetric data as described previously [ 20 ]. The protein partial specific volume was taken as 0.73 ml/g. assembly chaperone chaperone/usher pathway protein stability protein folding Most Gram-negative pathogens express adhesive surface...

View articletitled, Resolving the energy paradox of chaperone/usher-mediated fibre assembly

Articles

Truncated human serum albumin retains general anaesthetic binding activity

Renyu LIU, Jinsheng YANG, Chung-Eun HA, Nadhipuram V. BHAGAVAN, Roderic G. ECKENHOFF

Biochem J (2005) 388 (1): 39–45.

https://doi.org/10.1042/BJ20041224

Published: 10 May 2005

... at a wavelength of 275 nm. Emission spectra were recorded from 310 to 450 nm. anaesthesia anaesthetic halothane human serum albumin isothermal titration calorimetry protein stability It is now widely appreciated that many potential targets of inhaled anaesthetics have multiple binding sites...

View articletitled, Truncated human serum albumin retains general anaesthetic binding activity

Articles

Calcium-induced tertiary structure modifications of endo-β-1,3-glucanase from Pyrococcus furiosus in 7.9 M guanidinium chloride

Roberta CHIARALUCE, Giulio GIANESE, Sebastiana ANGELACCIO, Rita FLORIO, Johan F. T. van LIESHOUT, John van der OOST, Valerio CONSALVI

Biochem J (2005) 386 (3): 515–524.

https://doi.org/10.1042/BJ20041137

Published: 08 March 2005

... to this work. 2 To whom correspondence should be addressed (email [email protected] ). 2 7 2004 2 10 2004 14 10 2004 14 10 2004 The Biochemical Society, London 2005 calcium endo-β-1,3-glucanase guanidinium chloride homology modelling protein stability...

View articletitled, Calcium-induced tertiary structure modifications of endo-β-1,3-glucanase from Pyrococcus furiosus in 7.9 M guanidinium chloride

Articles

Contribution of the active-site metal cation to the catalytic activity and to the conformational stability of phosphotriesterase: temperature- and pH-dependence

Daniel ROCHU, Nathalie VIGUIÉ, Frédérique RENAULT, David CROUZIER, Marie-Thérèse FROMENT, Patrick MASSON

Biochem J (2004) 380 (3): 627–633.

https://doi.org/10.1042/bj20031861

Published: 15 June 2004

... electrophoresis metalloenzyme phosphotriesterase protein stability thermal unfolding Abbreviations used: BCA, bicinchoninic acid; CE, capillary electrophoresis; CHES, 2-( N -cyclohexylamino)ethanesulphonic acid; IEF, isoelectric focusing; OP, organophosphorus compounds; PTE, phosphotriesterase; RMSD...

View articletitled, Contribution of the active-site metal cation to the catalytic activity and to the conformational stability of phosphotriesterase: temperature- and pH-dependence

Articles

Conformational stability and warfarin-binding properties of human serum albumin studied by recombinant mutants

Hiroshi WATANABE, Ulrich KRAGH-HANSEN, Sumio TANASE, Keisuke NAKAJOU, Maki MITARAI, Yasunori IWAO, Toru MARUYAMA, Masaki OTAGIRI

Biochem J (2001) 357 (1): 269–274.

https://doi.org/10.1042/bj3570269

Published: 25 June 2001

... in affinity for binding of warfarin to H242Q and K199A and a smaller decrease in affinity for W214A and R218H. The findings show that Trp-214 is not as essential for the high-affinity binding ofwarfarin as has previously been thought. Key words: conformational changes, high-affinity binding, protein stability...

View articletitled, Conformational stability and warfarin-binding properties of human serum albumin studied by recombinant mutants

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Mitogen-activated protein kinases control p27/Kip1 expression and growth of human melanoma cells

Marcin KORTYLEWSKI, Peter C. HEINRICH, Maria-Elisabeth KAUFFMANN, Markus BÖHM, Andrzej MACKIEWICZ, Iris BEHRMANN

Biochem J (2001) 357 (1): 297–303.

https://doi.org/10.1042/bj3570297

Published: 25 June 2001

...-mail [email protected] ). 22 2 2001 26 3 2001 20 4 2001 The Biochemical Society, London ©2001 2001 PD098059 proliferation protein stability Abbreviations used: Cdk, cyclin-dependent kinase; ERK, extracellular signal-regulated protein kinase; GAPDH, glyceraldehyde-3...

View articletitled, Mitogen-activated protein kinases control p27/Kip1 expression and growth of human melanoma cells

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Alanine mutagenesis of high-mobility-group-protein-1 box B (HMG1-B)

Susann TAUDTE, Hong XIN, Neville R. KALLENBACH

Biochem J (2000) 347 (3): 807–814.

https://doi.org/10.1042/bj3470807

Published: 25 April 2000

... The Biochemical Society, London © 2000 2000 alanine scan four-way junction proteinDNA interaction protein stability structure-specific recognition Biochem. J. (2000) 347, 807 814 (Printed in Great Britain) 807 Alanine mutagenesis of high-mobility-group-protein-1 box B (HMG1-B) Susann TAUDTE, Hong XIN...

View articletitled, Alanine mutagenesis of high-mobility-group-protein-1 box B (HMG1-B)

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Electrostatic interactions affecting the active site of class Sigma glutathione S-transferase

Julie M. STEVENS, Richard N. ARMSTRONG, Heini W. DIRR

Biochem J (2000) 347 (1): 193–197.

https://doi.org/10.1042/bj3470193

Published: 27 March 2000

... protein stability protein unfolding Biochem. J. (2000) 347, 193 197 (Printed in Great Britain) 193 Electrostatic interactions affecting the active site of class Sigma glutathione S-transferase Julie M. STEVENS*, Richard N. ARMSTRONG and Heini W. DIRR*1 *Protein Structure Function Research Programme...

View articletitled, Electrostatic interactions affecting the active site of class Sigma glutathione S-transferase

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A possible origin of differences between calorimetric and equilibrium estimates of stability parameters of proteins

Amita SINHA, Sushma YADAV, Rizwan AHMAD, Faizan AHMAD

Biochem J (2000) 345 (3): 711–717.

https://doi.org/10.1042/bj3450711

Published: 25 January 2000

..., University of Delhi, New Delhi - 110 002, India. 3 To whom correspondence should be addressed (e-mail [email protected]). 28 7 1999 27 9 1999 12 11 1999 The Biochemical Society, London © 2000 2000 cytochrome c lysozyme myoglobin protein stability ribonuclease...

View articletitled, A possible origin of differences between calorimetric and equilibrium estimates of stability parameters of proteins

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Calorimetric studies on the stability of the ribosome-inactivating protein abrin II: effects of pH and ligand binding

Jayarapu KRUPAKAR, Chittoor P. SWAMINATHAN, Puspendu K. DAS, Avadhesha SUROLIA, Sunil K. PODDER

Biochem J (1999) 338 (2): 273–279.

https://doi.org/10.1042/bj3380273

Published: 22 February 1999

...-mail [email protected] ). 8 6 1998 27 10 1998 2 12 1998 The Biochemical Society, London © 1999 1999 differential scanning calorimetry isothermal titration calorimetry lactose binding protein stability thermal denaturation Biochem. J. (1999) 338, 273 279 (Printed...

View articletitled, Calorimetric studies on the stability of the ribosome-inactivating protein abrin II: effects of pH and ligand binding

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Epidermal growth factor increases the level of the cyclin-dependent kinase (CDK) inhibitor p21/CIP1 (CDK-interacting protein 1) in A431 cells by increasing the half-lives of the p21/CIP1 transcript and the p21/CIP1 protein

Lene E. JOHANNESSEN, Sigrun L. KNARDAL, Inger Helene MADSHUS

Biochem J (1999) 337 (3): 599–606.

https://doi.org/10.1042/bj3370599

Published: 25 January 1999

... 11 1998 The Biochemical Society, London © 1999 1999 epidermal growth factor receptor growth inhibition mRNA stability protein stability Biochem. J. (1999) 337, 599 606 (Printed in Great Britain) 599 Epidermal growth factor increases the level of the cyclin-dependent kinase (CDK...

View articletitled, Epidermal growth factor increases the level of the cyclin-dependent kinase (CDK) inhibitor p21/CIP1 (CDK-interacting protein 1) in A431 cells by increasing the half-lives of the p21/CIP1 transcript and the p21/CIP1 protein

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