...: Carlos M Farinha ( [email protected] ) actin cytoskeleton cAMP CFTR EPAC1 protein trafficking Cystic Fibrosis (CF) is the most common lethal and life-limiting autosomic recessive disorder among Caucasian population, affecting about 1 : 2500–6000 new borns [ 1 ] and it is caused...

... lysis buffer was obtained from Merck Millipore. 30 3 2017 3 7 2017 20 7 2017 21 7 2017 © 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2017 irisin secretion N-linked glycosylation protein stabilization protein...

...) exchanger 1 (kAE1) can cause genetic distal renal tubular acidosis (dRTA). Different SLC4A1 mutations give rise to mutant kAE1 proteins with distinct defects in protein trafficking. The mutant kAE1 protein may be retained in endoplasmic reticulum (ER) or Golgi apparatus, or mis-targeted to the apical...

.... As for many other membrane proteins, its intracellular sorting and distribution have a tremendous effect on its function, and constitute an important aspect of its regulation. Many short sequences have been shown to contribute to protein trafficking along the sorting or endocytic pathways. Using...

... migration and invasion. annexin 2 caveolae caveolin-1 enolase-1 invasion migration protein trafficking The PLG/PLA (plasminogen/plasmin) system has been linked to a variety of pericellular proteolytic processes in embryonic development [ 1 ], monocyte/macrophage recruitment [ 2 , 3...

... and participates during rescue of a common disease-associated mutation. Therapeutic strategies to overcome clinically important defects in cystic fibrosis should consider the role of palmitoylation as a molecular target. cystic fibrosis palmitoylation post-translational modification protein trafficking...

... Journal compilation © 2013 Biochemical Society 2013 genotype–phenotype relationship human ethera-go-go -related gene KCNH2 long-QT syndrome protein trafficking Loss-of-function mutations in membrane-spanning proteins usually result in protein misfolding, which leads to enhanced...

... effects were observed in cells enriched with lipoprotein-derived cholesterol and in primary human macrophages. Removal of excess cholesterol by a cyclodextrin vehicle restored apoE secretion, indicating that the transport defect was reversible. That cholesterol impaired protein trafficking was supported...

.... doppel (Dpl) epithelial cell lipid raft prion protein (PrP) protein trafficking To obtain singly PrP C -expressing clones, FRT and MDCK cells were stably transfected with a cDNA encoding 3F4-tagged murine (mo) PrP C (a gift from Dr S. Lehmann, Centre National de la Recherche Scientifique...

... drugs (e.g. [ 5 ]). cystic fibrosis transmembrane conductance regulator (CFTR) ΔF508 rescue protein trafficking streptavidin pulldown Figure 3 Biotinylation by low concentrations of sulfo-NHS-SS-biotin Western blot showing the amount of R domain (RD) precipitated on streptavidin–agarose...

... Biochemical Society 2007 digestive vacuole green fluorescent protein (GFP) pH plasmepsin Plasmodium falciparum protein trafficking As a consequence of this diet of Hb, the parasite produces large amounts of free haem. Haem is a toxic molecule that can disrupt membranes, inhibit...

... precursor cell expressed developmentally down-regulated gene 4-2 (Nedd4-2) protein trafficking ubiquitin The ENaC (epithelial sodium channel) is a protein complex composed of three subunits: α, β and γ [ 1 , 2 ]. ENaC is expressed in the apical membrane of epithelia found in the lung, colon...

...) prenylated Rab acceptor 1 (PRA1) prenylin protein trafficking Yip3 Abbreviations used: BHK, baby hamster kidney; BMH, bismaleimidohexane; DSS, disuccinyl suberate; ER, endoplasmic reticulum; GFP, green fluorescent protein; Man II, mannosidase II; WT, wild-type; D176A etc., Asp 176 →Ala...

... LSB6 phosphoinositide protein trafficking yeast Abbreviations used: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GFP, green fluorescent protein; HA, haemagglutinin; LSB6 , L a s 17p- b inding protein; ORF, open reading frame; PI 4-kinase, phosphoinositide 4-kinase; PI4KII, PI 4...

... assembly membrane integration protein trafficking Abbreviations used: Cx, connexin; DTT, dithiothreitol; TEA, triethanolamine. Biochem. J. (2002) 365, 693 699 (Printed in Great Britain) 693 Post-translational integration and oligomerization of connexin 26 in plasma membranes and evidence...

... (e-mail [email protected] ). 15 11 2000 24 1 2001 19 2 2001 The Biochemical Society, London © 2001 2001 amino acid transport heterodimeric transporter protein trafficking Biochem. J. (2001) 355, 725 731 (Printed in Great Britain) 725 Association of 4F2hc...

... correspondence should be addressed (e-mail [email protected] ). 27 10 2000 8 1 2001 14 2 2001 The Biochemical Society, London © 2001 2001 ATP-binding cassette protein multidrug resistance protein trafficking Biochem. J. (2001) 355, 617 624 (Printed in Great Britain...

... to GenBank under the accession numbers AF230802 and AF230803 respectively. 24 2 2000 23 6 2000 19 7 2000 The Biochemical Society, London © 2000 2000 calcium protein trafficking receptor-stimulated channel store-operated channel vomeronasal organ Biochem. J. (2000) 351, 115...

... internalization protein trafficking zinc uptake Biochem. J. (2000) 346, 329 336 (Printed in Great Britain) 329 Zinc-regulated ubiquitin conjugation signals endocytosis of the yeast ZRT1 zinc transporter Raad S. GITAN and David J. EIDE1 Department of Nutritional Sciences, 217 Gwynn Hall, University...