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Articles

Charting the importance of filamin A posttranslational modifications

Kyle D. Shead, Veneta Salyahetdinova, George S. Baillie

Biochem J (2024) 481 (13): 865–881.

https://doi.org/10.1042/BCJ20240121

Published: 03 July 2024

... (CC BY) . filamin A phosphorylation post translational modification proteolysis The filamins are a group of actin binding, scaffold proteins consisting of three isoforms, Filamin A, B and C. Filamin A (previously termed actin binding protein-280, ABP-280) was the first isoform...

View article titled, Charting the importance of filamin A posttranslational modifications

Articles

Non-cross-linking advanced glycation end products affect prohormone processing

Sebastian Brings, Walter Mier, Barbro Beijer, Elisabeth Kliemank, Stephan Herzig, Julia Szendroedi, Peter P. Nawroth, Thomas Fleming

Biochem J (2024) 481 (1): 33–44.

https://doi.org/10.1042/BCJ20230321

Published: 04 January 2024

...). Published by Portland Press Limited on behalf of the Biochemical Society 2024 advanced glycation end products diabetes glycation insulin proteolysis solid-phase peptide synthesis The modification of amino acids by hexoses and dicarbonyls can result in the formation of protein-bound...

View article titled, Non-cross-linking advanced glycation end products affect prohormone processing

Includes: Supplementary data

Articles

Catalysis of non-canonical protein ubiquitylation by the ARIH1 ubiquitin ligase

Nicholas Purser, Ishita Tripathi-Giesgen, Jerry Li, Daniel C. Scott, Daniel Horn-Ghetko, Kheewoong Baek, Brenda A. Schulman, Arno F. Alpi, Gary Kleiger

Biochem J (2023) 480 (22): 1817–1831.

https://doi.org/10.1042/BCJ20230373

Published: 17 November 2023

... to this work. 31 8 2023 19 10 2023 23 10 2023 © 2023 The Author(s) 2023 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . proteolysis ubiquitin...

View article titled, Catalysis of non-canonical protein ubiquitylation by the ARIH1 ubiquitin ligase

Includes: Supplementary data

Articles

The C-terminal region of phytoene synthase is a key element to control carotenoid biosynthesis in the haloarchaeon Haloferax volcanii

Micaela Cerletti, Agustín Rabino, Roberto A. Paggi, Celeste Ferrari, Ansgar Poetsch, Harri Savilahti, Saija Kiljunen, Rosana E. De Castro

Biochem J (2022) 479 (22): 2365–2377.

https://doi.org/10.1042/BCJ20220403

Published: 23 November 2022

... degradation assays showed that HvPSY34 was more stable than HvPSYwt. Collectively, these results show that the C-terminal region of HvPSY contains a ‘recognition determinant’ for proteolysis in H. volcanii . Preliminary evidence suggests that LonB is involved in the recognition mechanism. This study provides...

View article titled, The C-terminal region of phytoene synthase is a key element to control carotenoid biosynthesis in the haloarchaeon Haloferax volcanii

Includes: Supplementary data

Articles

Binding and inhibition of the ternary complex factor Elk-4/Sap1 by the adapter protein Dok-4

Erika Hooker, Cindy Baldwin, Victoria Roodman, Anupam Batra, Naajia Nur Isa, Tomoko Takano, Serge Lemay

Biochem J (2017) 474 (9): 1509–1528.

https://doi.org/10.1042/BCJ20160832

Published: 19 April 2017

... possible interactions were screened with and without co-expression of Lyn tyrosine kinase, respectively. adaptor proteins protein–protein interactions proteolysis transcription factors The Dok (downstream of kinase) family of adapter proteins consists of seven related proteins characterized...

View article titled, Binding and inhibition of the ternary complex factor Elk-4/Sap1 by the adapter protein Dok-4

Includes: Supplementary data

Articles

Combinatorial protein engineering of proteolytically resistant mesotrypsin inhibitors as candidates for cancer therapy

Itay Cohen, Olumide Kayode, Alexandra Hockla, Banumathi Sankaran, Derek C. Radisky, Evette S. Radisky, Niv Papo

Biochem J (2016) 473 (10): 1329–1341.

https://doi.org/10.1042/BJ20151410

Published: 11 May 2016

... and identifies a promising mesotrypsin inhibitor as a starting point for the development of anticancer protein therapeutics. cancer therapy directed evolution enzyme inhibition mesotrypsin protease inhibitor protein engineering proteolysis X-ray structure The human serine protease...

View article titled, Combinatorial protein engineering of proteolytically resistant mesotrypsin inhibitors as candidates for cancer therapy

Includes: Supplementary data

Articles

Structure-based design and functional studies of novel noroviral 3C protease chimaeras offer insights into substrate specificity

Morgan R. Herod, Cynthia A. Prince, Rachel J. Skilton, Vernon K. Ward, Jonathan B. Cooper, Ian N. Clarke

Biochem J (2014) 464 (3): 461–472.

https://doi.org/10.1042/BJ20140959

Published: 05 December 2014

... and domain 2 of the norovirus NS6 protease influences cleavage at specific non-structural substrate boundaries. murine norovirus (MNV) norovirus protease polyprotein positive sense proteolysis Noroviruses are members of the calicivirus family of positive-sense, single-stranded RNA viruses...

View article titled, Structure-based design and functional studies of novel noroviral 3C protease chimaeras offer insights into substrate specificity

Includes: Supplementary data

Articles

Zymogen activation of neurotrypsin and neurotrypsin-dependent agrin cleavage on the cell surface are enhanced by glycosaminoglycans

Claudio Gisler, Daniel Lüscher, Philipp Schätzle, Stephanie Dürr, Antonio Baici, Giovanna Galliciotti, Raymond Reif, Marc F. Bolliger, Beat Kunz, Peter Sonderegger

Biochem J (2013) 453 (1): 83–100.

https://doi.org/10.1042/BJ20130166

Published: 13 June 2013

... compilation © 2013 Biochemical Society 2013 agrin glycosaminoglycan (GAG) neurotrypsin proprotein convertase proteolysis zymogen activation The synaptic serine peptidase neurotrypsin plays an important role in higher brain functions, as humans with a defect in neurotrypsin exhibit severe...

View article titled, Zymogen activation of neurotrypsin and neurotrypsin-dependent agrin cleavage on the cell surface are enhanced by glycosaminoglycans

Includes: Supplementary data

Articles

The mannose 6-phosphate-binding sites of M6P/IGF2R determine its capacity to suppress matrix invasion by squamous cell carcinoma cells

Olivia C. Probst, Evren Karayel, Nicole Schida, Elisabeth Nimmerfall, Elisabeth Hehenberger, Verena Puxbaum, Lukas Mach

Biochem J (2013) 451 (1): 91–99.

https://doi.org/10.1042/BJ20121422

Published: 14 March 2013

...-like growth factor (IGF) lysosome mannose 6-phosphate (M6P) proteolysis Total cellular membranes were resuspended in 100 μl of binding buffer [0.15 M NaCl, 50 mM imidazole/HCl (pH 7.0) and 0.02% NaN 3 ] containing proteinase inhibitors and extracted with 1% (w/v) Triton X-100 for 30 min...

View article titled, The mannose 6-phosphate-binding sites of M6P/IGF2R determine its capacity to suppress matrix invasion by squamous cell carcinoma cells

Includes: Supplementary data

Articles

Glucose induces autophagy under starvation conditions by a p38 MAPK-dependent pathway

Jose Félix Moruno-Manchón, Eva Pérez-Jiménez, Erwin Knecht

Biochem J (2013) 449 (2): 497–506.

https://doi.org/10.1042/BJ20121122

Published: 14 December 2012

... (MAPK) proteolysis Equilibrium between anabolic and catabolic processes preserves the homoeostasis necessary to ensure normal cell growth and development. Upon nutrient deprivation or cellular stress, catabolic processes prevail in order to supply the energy required to maintain cell...

View article titled, Glucose induces autophagy under starvation conditions by a p38 MAPK-dependent pathway

Includes: Supplementary data

Articles

Structure–function relationships in calpains

Robert L. Campbell, Peter L. Davies

Biochem J (2012) 447 (3): 335–351.

https://doi.org/10.1042/BJ20120921

Published: 05 October 2012

... © The Authors Journal compilation © 2012 Biochemical Society 2012 calcium-binding domain structure enzyme activation inhibitor complex proteinase proteolysis X-ray crystallography The present review will focus on insights about calpains and their functioning that have...

View article titled, Structure–function relationships in calpains

Includes: Multimedia, Supplementary data

Articles

MMP proteolysis of the human extracellular matrix protein aggrecan is mainly a process of normal turnover

André Struglics, Maria Hansson

Biochem J (2012) 446 (2): 213–223.

https://doi.org/10.1042/BJ20120274

Published: 14 August 2012

...André Struglics; Maria Hansson Although it has been shown that aggrecanases are involved in aggrecan degradation, the role of MMP (matrix metalloproteinase) aggrecanolysis is less well studied. To investigate MMP proteolysis of human aggrecan, in the present study we used neoepitope antibodies...

View article titled, MMP proteolysis of the human extracellular matrix protein aggrecan is mainly a process of normal turnover

Includes: Supplementary data

Articles

Interaction specificity between the chaperone and proteolytic components of the cyanobacterial Clp protease

Anders Tryggvesson, Frida M. Ståhlberg, Axel Mogk, Kornelius Zeth, Adrian K. Clarke

Biochem J (2012) 446 (2): 311–320.

https://doi.org/10.1042/BJ20120649

Published: 14 August 2012

... were analysed by PAGE on 12% BisTris gels using the NuPAGE gel system (Invitrogen). Proteins were stained using the colloidal Coomassie Blue method. chloroplast Clp protein cyanobacterium proteolysis Proteolysis performs a vital homoeostatic role in all organisms, within which energy...

View article titled, Interaction specificity between the chaperone and proteolytic components of the cyanobacterial Clp protease

Includes: Supplementary data

Articles

Disrupted in renal carcinoma 2 (DIRC2), a novel transporter of the lysosomal membrane, is proteolytically processed by cathepsin L

Lalu Rudyat Telly Savalas, Bruno Gasnier, Markus Damme, Torben Lübke, Christian Wrocklage, Cécile Debacker, Adrien Jézégou, Thomas Reinheckel, Andrej Hasilik, Paul Saftig, Bernd Schröder

Biochem J (2011) 439 (1): 113–128.

https://doi.org/10.1042/BJ20110166

Published: 14 September 2011

... of the proteolysis site, internal HA tags were introduced into DIRC2–3×Myc-pcDNA3.1/Hygro+ after amino acids 214 and 261 of the DIRC2 ORF respectively, by overlap-extension PCR. As internal primers HA214-FW (5′-CATACGACGTCCCAGACTACGCTCTTGCTGCAGAGAGCAGCAG-3′) and HA214-Rv (5...

View article titled, Disrupted in renal carcinoma 2 (DIRC2), a novel transporter of the lysosomal membrane, is proteolytically processed by cathepsin L

Includes: Supplementary data

Articles

Cleavage of Notch1 by granzyme B disables its transcriptional activity

Geert van Tetering, Niels Bovenschen, Jan Meeldijk, Paul J. van Diest, Marc Vooijs

Biochem J (2011) 437 (2): 313–322.

https://doi.org/10.1042/BJ20110226

Published: 28 June 2011

...), resulting in a heterodimeric receptor. Upon ligand binding at the cell surface, Notch receptors undergo two successive proteolytic cleavages: an ectodomain cleavage (S2), followed by intramembrane proteolysis by γ-secretase (S3; Figure 1 A) [ 5 ]. This process releases the NICD (Notch intracellular domain...

View article titled, Cleavage of Notch1 by granzyme B disables its transcriptional activity

Includes: Supplementary data

Articles

Probing cationic selectivity of cardiac calsequestrin and its CPVT mutants

Naresh C. Bal, Nivedita Jena, Danesh Sopariwala, Tuniki Balaraju, Sana Shaikh, Chandralata Bal, Ashoke Sharon, Sandor Gyorke, Muthu Periasamy

Biochem J (2011) 435 (2): 391–399.

https://doi.org/10.1042/BJ20101771

Published: 29 March 2011

...+ (having higher affinity for CASQ2 than Ca 2+ ) was used to achieve molecular compaction at 0.2 mM, then Ca 2+ was added and turbidity was measured as described above. All turbidity measurements were repeated at least three times. Purified WT and mutants of CASQ2 were subjected to proteolysis...

View article titled, Probing cationic selectivity of cardiac calsequestrin and its CPVT mutants

Includes: Supplementary data

Articles

Calpain is involved in C-terminal truncation of human aggrecan

André Struglics, Maria Hansson

Biochem J (2010) 430 (3): 531–538.

https://doi.org/10.1042/BJ20100591

Published: 27 August 2010

... protease, has a specific role in the proteolysis of aggrecan we developed neoepitope antibodies (anti-PGVA, anti-GDLS and anti-EDLS) against calpain cleavage sites and used Western blot analysis to identify calpain-generated fragments in normal and OA (osteoarthritis) knee cartilage and SF (synovial fluid...

View article titled, Calpain is involved in C-terminal truncation of human aggrecan

Includes: Supplementary data

Articles

Proteolytic processing of an Arabidopsis membrane-bound NAC transcription factor is triggered by cold-induced changes in membrane fluidity

Pil Joon Seo, Mi Jung Kim, Jin-Su Song, Youn-Sung Kim, Hie-Joon Kim, Chung-Mo Park

Biochem J (2010) 427 (3): 359–367.

https://doi.org/10.1042/BJ20091762

Published: 14 April 2010

... and proteases showed that NTL6 processing occurs via the regulated intramembrane proteolysis mechanism. Interestingly, a metalloprotease inhibitor blocked the NTL6 processing. These observations indicate that a metalloprotease activity is responsible for NTL6 processing in response to cold-induced changes...

View article titled, Proteolytic processing of an Arabidopsis membrane-bound NAC transcription factor is triggered by cold-induced changes in membrane fluidity

Articles

Variably modulated gating of the 26S proteasome by ATP and polyubiquitin

Xiaohua Li, George N. Demartino

Biochem J (2009) 421 (3): 397–404.

https://doi.org/10.1042/BJ20090528

Published: 15 July 2009

... that requires bound ATP to maintain its component subcomplexes, the 20S proteasome and PA700, in a functionally assembled state. Proteolysis of both types of substrate requires PA700-induced opening of reversible gates at substrate-access pores of the 20S proteasome. In the present study we demonstrate...

View article titled, Variably modulated gating of the 26S proteasome by ATP and polyubiquitin

Articles

Substrate specificity of transthyretin: identification of natural substrates in the nervous system

Márcia A. Liz, Carolina E. Fleming, Ana F. Nunes, Maria R. Almeida, Fernando M. Mar, Youngchool Choe, Charles S. Craik, James C. Powers, Matthew Bogyo, Mónica M. Sousa

Biochem J (2009) 419 (2): 467–474.

https://doi.org/10.1042/BJ20082090

Published: 27 March 2009

... neurite outgrowth neuropeptide Y (NPY) phosphonate inhibitor proteolysis substrate specificity transthyretin In the present study, we investigated the substrate specificity of TTR and assessed whether some of the phenotypes described in TTR-KO mice, namely TTR involvement in PAM expression...

View article titled, Substrate specificity of transthyretin: identification of natural substrates in the nervous system

Articles

Identification of new protein substrates for the chloroplast ATP-dependent Clp protease supports its constitutive role in Arabidopsis

Tara M. Stanne, Lars L. E. Sjögren, Shai Koussevitzky, Adrian K. Clarke

Biochem J (2009) 417 (1): 257–269.

https://doi.org/10.1042/BJ20081146

Published: 12 December 2008

... 29 8 2008 29 8 2008 © The Authors Journal compilation © 2009 Biochemical Society 2009 Arabidopsis thaliana chloroplast Clp proteolysis stroma Molecular chaperones and proteases are integral components of the quality control processes in the actively changing cellular...

View article titled, Identification of new protein substrates for the chloroplast ATP-dependent Clp protease supports its constitutive role in Arabidopsis

Includes: Supplementary data

Articles

Topology mapping of the vacuolar Vcx1p Ca 2+ /H + exchanger from Saccharomyces cerevisiae

Verónica A. Segarra, Lise Thomas

Biochem J (2008) 414 (1): 133–141.

https://doi.org/10.1042/BJ20080364

Published: 29 July 2008

... Vcx1p's topology using three biochemical assays: C-terminal reporter localization, glycosylation mapping and proteolysis. We have found that the protein has an odd number of TM (transmembrane) domains and that its termini are located on opposite sides of the membrane, with the N-terminus in the cytoplasm...

View article titled, Topology mapping of the vacuolar Vcx1p Ca 2+ /H + exchanger from Saccharomyces cerevisiae

Includes: Supplementary data

Articles

Catecholaminergic polymorphic ventricular tachycardia-related mutations R33Q and L167H alter calcium sensitivity of human cardiac calsequestrin

Giorgia Valle, Daniela Galla, Alessandra Nori, Silvia G. Priori, Sandor Gyorke, Vincenzo de Filippis, Pompeo Volpe

Biochem J (2008) 413 (2): 291–303.

https://doi.org/10.1042/BJ20080163

Published: 26 June 2008

... by spectroscopic (CD and fluorescence) and biochemical (size-exclusion chromatography and limited proteolysis) methods at 500 and 100 mM KCl, with or without Ca 2+ at a physiological intraluminal concentration of 1 mM; Ca 2+ -induced polymerization properties were studied by turbidimetry. In the absence of Ca 2...

View article titled, Catecholaminergic polymorphic ventricular tachycardia-related mutations R33Q and L167H alter calcium sensitivity of human cardiac calsequestrin

Articles

Mutational analysis of conserved glycine residues 142, 143 and 146 reveals Gly 142 is critical for tetramerization of CTP synthase from Escherichia coli

Faylene A. Lunn, Travis J. MacLeod, Stephen L. Bearne

Biochem J (2008) 412 (1): 113–121.

https://doi.org/10.1042/BJ20071163

Published: 25 April 2008

... [ 6 ]. Scheme 1 CTP-forming reactions and glutaminase reaction catalysed by CTPS amidotransferase CTP glycine mutagenesis proteolysis synthase tetramerization UTP CTPS from Escherichia coli is an excellent model for understanding catalysis by CTPSs from other organisms...

View article titled, Mutational analysis of conserved glycine residues 142, 143 and 146 reveals Gly 142 is critical for tetramerization of CTP synthase from Escherichia coli

Includes: Supplementary data

Articles

The human fibrinolytic system is a target for the staphylococcal metalloprotease aureolysin

Nathalie Beaufort, Piotr Wojciechowski, Christian P. Sommerhoff, Grzegorz Szmyd, Grzegorz Dubin, Sigrun Eick, Josef Kellermann, Manfred Schmitt, Jan Potempa, Viktor Magdolen

Biochem J (2008) 410 (1): 157–165.

https://doi.org/10.1042/BJ20070650

Published: 29 January 2008

..., (ii) the cathelicidin-derived antimicrobial peptide, LL-37, and (iii) the serpin-type plasma protease inhibitors α 1 -antichymotrypsin and α 1 -proteinase inhibitor, thereby potentially promoting bacterial escape from the host immune system, as well as uncontrolled proteolysis, leading to host tissue...

View article titled, The human fibrinolytic system is a target for the staphylococcal metalloprotease aureolysin

Articles

Human iron regulatory protein 2 is easily cleaved in its specific domain: consequences for the haem binding properties of the protein

Camille Dycke, Catherine Bougault, Jacques Gaillard, Jean-Pierre Andrieu, Kostas Pantopoulos, Jean-Marc Moulis

Biochem J (2007) 408 (3): 429–439.

https://doi.org/10.1042/BJ20070983

Published: 28 November 2007

...-Domain are sensitive to proteolysis at the same site. NMR results suggest that the isolated 73aa-Domain is not structured. Iron-independent cleavage of IRP2 within the 73aa-Domain also occurs in lung cancer (H1299) cells. Haem interacts with a cysteine residue only in truncated forms of the 73aa-Domain...

View article titled, Human iron regulatory protein 2 is easily cleaved in its specific domain: consequences for the haem binding properties of the protein

Includes: Supplementary data

Articles

Urinary-type plasminogen activator (uPA) and its receptor (uPAR) in squamous cell carcinoma of the oral cavity

Zonggao Shi, M. Sharon Stack

Biochem J (2007) 407 (2): 153–159.

https://doi.org/10.1042/BJ20071037

Published: 25 September 2007

...) proteinase proteolysis urinary-type plasminogen activator (uPA) uPA receptor (uPAR) OSCC (oral squamous cell carcinoma), including tumours affecting the tongue, floor of the mouth, buccal mucosa, lips, palate and gingiva, is the most common malignancy of the oral cavity, causing more deaths than...

View article titled, Urinary-type plasminogen activator (uPA) and its receptor (uPAR) in squamous cell carcinoma of the oral cavity

Articles

Finding the needles in the haystack: mapping constitutive proteolytic events in vivo

Matthew Bogyo

Biochem J (2007) 407 (1): e1.

https://doi.org/10.1042/BJ20071096

Published: 12 September 2007

... that this methodology provides a comprehensive map of constitutive trimming events mediated by regulatory proteases such as methionine aminopeptidase. This study also identifies previously uncharacterized processing events that highlight potential novel regulatory mechanisms mediated by proteolysis. mass...

View article titled, Finding the needles in the haystack: mapping constitutive proteolytic events in vivo

Articles

STAT5 isoforms: controversies and clarifications

Haydeé L. Ramos, John J. O'Shea, Wendy T. Watford

Biochem J (2007) 404 (1): e1.

https://doi.org/10.1042/BJ2007420

Published: 26 April 2007

.... A. Chong H. J. Ryan J. J. Mast cell-restricted p70 Stat6 isoform is a product of selective proteolysis Cytokine 2002 19 218 227 22 Piazza F. Valens J. Lagasse E. Schindler C. Myeloid differentiation of FdCP1 cells is dependent on Stat5 processing Blood 2000 96...

View article titled, STAT5 isoforms: controversies and clarifications

Articles

Proteinase K-sensitive disease-associated ovine prion protein revealed by conformation-dependent immunoassay

Alana M. Thackray, Lee Hopkins, Raymond Bujdoso

Biochem J (2007) 401 (2): 475–483.

https://doi.org/10.1042/BJ20061264

Published: 21 December 2006

...Alana M. Thackray; Lee Hopkins; Raymond Bujdoso PrPSc [abnormal disease-specific conformation of PrP (prion-related protein)] accumulates in prion-affected individuals in the form of amorphous aggregates. Limited proteolysis of PrPSc results in a protease-resistant core of PrPSc of molecular mass...

View article titled, Proteinase K-sensitive disease-associated ovine prion protein revealed by conformation-dependent immunoassay

Articles

Cleavage, a real turn-off? HIV-mediated proteolysis of PABP1

Brian Collier, Nicola K. Gray

Biochem J (2006) 396 (2): e9.

https://doi.org/10.1042/BJ20060545

Published: 15 May 2006

...–viral interaction poly(A)-binding protein (PABP) proteolysis Translational control of mRNAs is central to the regulation of gene expression and is important in the aetiology of human disease. Many viruses have evolved complex mechanisms to inhibit host cellular mRNA translation while facilitating...

View article titled, Cleavage, a real turn-off? HIV-mediated proteolysis of PABP1

Articles

Insulin-like growth factor-binding protein-5 (IGFBP-5): a critical member of the IGF axis

James Beattie, Gordon J. Allan, Jennifer D. Lochrie, David J. Flint

Biochem J (2006) 395 (1): 1–19.

https://doi.org/10.1042/BJ20060086

Published: 15 March 2006

... to two excellent reviews [ 22 , 23 ]. For a short review on some of the properties of IGFBP-5, see [ 24 ]. extracellular matrix (ECM) glycosaminoglycan insulin-like growth factor-I (IGF-I) insulin-like growth factor-binding protein 5 (IGFBP-5) mammary gland proteolysis The IGF (insulin...

View article titled, Insulin-like growth factor-binding protein-5 (IGFBP-5): a critical member of the IGF axis

Articles

Role of N-terminal residues in the ubiquitin-independent degradation of human thymidylate synthase

Maria Marjorette O. Peña, Yang Yang Xing, Sangita Koli, Franklin G. Berger

Biochem J (2006) 394 (1): 355–363.

https://doi.org/10.1042/BJ20051479

Published: 27 January 2006

... proteasome in a ubiquitin-independent manner. Such degradation is directed by the disordered N-terminal region of the TS polypeptide, and is abrogated by ligand binding. In the present study, we have verified the ubiquitin-independent nature of TS proteolysis by showing that a ‘lysine-less’ polypeptide...

View article titled, Role of N-terminal residues in the ubiquitin-independent degradation of human thymidylate synthase

Articles

Endocytic processing of connexin43 gap junctions: a morphological study

Edward Leithe, Andreas Brech, Edgar Rivedal

Biochem J (2006) 393 (1): 59–67.

https://doi.org/10.1042/BJ20050674

Published: 12 December 2005

... endocytosis gap junction PMA proteolysis Neighbouring cells are able to exchange ions, metabolites and cell-signalling molecules of <1 kDa via hydrophilic channels composed of proteins called connexins [ 1 ]. Connexins are tetramembrane-spanning proteins that oligomerize into hemichannels [ 2...

View article titled, Endocytic processing of connexin43 gap junctions: a morphological study

Articles

Conformation-dependent inactivation of human betaine-homocysteine S-methyltransferase by hydrogen peroxide in vitro

Catherine M. Miller, Sandra S. Szegedi, Timothy A. Garrow

Biochem J (2005) 392 (3): 443–448.

https://doi.org/10.1042/BJ20050356

Published: 06 December 2005

... structure. Oxidation is attenuated when both binding sites are occupied by CBHcy, but Met alone has no effect. Partial digestion of ligand-free BHMT with trypsin produces two large peptides, excising a seven-residue peptide within loop L2. CBHcy but not Met binding slows down proteolysis by trypsin...

View article titled, Conformation-dependent inactivation of human betaine-homocysteine S-methyltransferase by hydrogen peroxide in vitro

Articles

Regulation of the localization and activity of inositol 1,4,5-trisphosphate 3-kinase B in intact cells by proteolysis

Jowie C. H. Yu, Samantha M. Lloyd-Burton, Robin F. Irvine, Michael J. Schell

Biochem J (2005) 392 (3): 435–441.

https://doi.org/10.1042/BJ20050829

Published: 06 December 2005

... we have investigated the localization and activity of IP3KB and its modulation by proteolysis. We found that the N- and C-termini (either side of residue 262) of IP3KB localized predominantly to the actin cytoskeleton and ER (endoplasmic reticulum) respectively, both in COS-7 cells and in primary...

View article titled, Regulation of the localization and activity of inositol 1,4,5-trisphosphate 3-kinase B in intact cells by proteolysis

Articles

Plasmin alters the activity and quaternary structure of human plasma carboxypeptidase N

Mercy O. QUAGRAINE, Fulong TAN, Hironori TAMEI, Ervin G. ERDÖS, Randal A. SKIDGEL

Biochem J (2005) 388 (1): 81–91.

https://doi.org/10.1042/BJ20041471

Published: 10 May 2005

... active tetrameric form of human CPN in plasma. Although it is known that CPN is sensitive to cleavage by plasmin and other serine proteases [ 16 , 27 ], the cleavage sites in CPN have never been identified and the effects of proteolysis on the activity and quaternary structure of CPN have not been...

View article titled, Plasmin alters the activity and quaternary structure of human plasma carboxypeptidase N

Articles

A proteolytic fragment of Mcl-1 exhibits nuclear localization and regulates cell growth by interaction with Cdk1

Sarwat JAMIL, Rafat SOBOUTI, Payman HOJABRPOUR, Meera RAJ, Juergen KAST, Vincent DURONIO

Biochem J (2005) 387 (3): 659–667.

https://doi.org/10.1042/BJ20041596

Published: 26 April 2005

... cell leukaemia-1 (Mcl-1) nuclear localization proteolysis The Bcl-2 family of proteins are known regulators of cell death and survival, acting as regulators of membrane integrity at both the mitochondria and the endoplasmic reticulum [ 1 – 5 ]. They are characterized by the presence of one...

View article titled, A proteolytic fragment of Mcl-1 exhibits nuclear localization and regulates cell growth by interaction with Cdk1

Articles

Cleavage at the stem region releases an active ectodomain of the membrane type 1 matrix metalloproteinase

Marta TOTH, Pamela OSENKOWSKI, Dusan HESEK, Stephen BROWN, Samy MEROUEH, Wael SAKR, Shahriar MOBASHERY, Rafael FRIDMAN

Biochem J (2005) 387 (2): 497–506.

https://doi.org/10.1042/BJ20041324

Published: 05 April 2005

..., London 2005 ectodomain matrix metalloproteinase (MMP) shedding protease proteolysis tissue inhibitor of metalloproteinase (TIMP) MT1-MMP (membrane type 1 matrix metalloproteinase) is a type I membrane-anchored MMP that has been shown to play a critical role in the pericellular...

View article titled, Cleavage at the stem region releases an active ectodomain of the membrane type 1 matrix metalloproteinase

Includes: Supplementary data

Articles

Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes

Nabila ABOULAICH, Julia P. VAINONEN, Peter STRÅLFORS, Alexander V. VENER

Biochem J (2004) 383 (2): 237–248.

https://doi.org/10.1042/BJ20040647

Published: 08 October 2004

... identification of proteins at the opposite membrane surfaces by proteolysis and MS. Extracellular-exposed caveolae-specific proteins CD36 and copper-containing amine oxidase were concealed inside the vesicles and resisted trypsin treatment. The cytosol-orientated caveolins were efficiently digested by trypsin...

View article titled, Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes

Articles

Class III phosphoinositide 3-kinase–Beclin1 complex mediates the amino acid-dependent regulation of autophagy in C2C12 myotubes

Amina TASSA, Marie Paule ROUX, Didier ATTAIX, Daniel M. BECHET

Biochem J (2003) 376 (3): 577–586.

https://doi.org/10.1042/bj20030826

Published: 15 December 2003

...Amina TASSA; Marie Paule ROUX; Didier ATTAIX; Daniel M. BECHET Increased proteolysis contributes to muscle atrophy that prevails in many diseases. Elucidating the signalling pathways responsible for this activation is of obvious clinical importance. Autophagy is a ubiquitous degradation process...

View article titled, Class III phosphoinositide 3-kinase–Beclin1 complex mediates the amino acid-dependent regulation of autophagy in C2C12 myotubes

Articles

The mouse matrix metalloproteinase, epilysin (MMP-28), is alternatively spliced and processed by a furin-like proprotein convertase

Sara A. ILLMAN, Jorma KESKI-OJA, William C. PARKS, Jouko LOHI

Biochem J (2003) 375 (1): 191–197.

https://doi.org/10.1042/bj20030497

Published: 01 October 2003

... in intact, healthy tissues suggests that this MMP functions in physiological tissue homoeostasis and turnover. Key words: alternative splicing, extracellular matrix, matrix metalloproteinase, proteolysis, tissue expression. INTRODUCTION The matrix metalloproteinases (MMPs) comprise a family of at least 21...

View article titled, The mouse matrix metalloproteinase, epilysin (MMP-28), is alternatively spliced and processed by a furin-like proprotein convertase

Articles

Changes in the proteolytic activities of proteasomes and lysosomes in human fibroblasts produced by serum withdrawal, amino-acid deprivation and confluent conditions

Graciela FUERTES, José Javier MARTÍN DE LLANO, Adoración VILLARROYA, A. Jennifer RIVETT, Erwin KNECHT

Biochem J (2003) 375 (1): 75–86.

https://doi.org/10.1042/bj20030282

Published: 01 October 2003

... that the activity of the various pro- teolytic pathways depends on the growth conditions of cells and will be useful for investigation of the specific signals that control their activity. Key words: lysosome, macroautophagy, protease, proteasome, proteolysis. INTRODUCTION In both prokaryotic and eukaryotic cells...

View article titled, Changes in the proteolytic activities of proteasomes and lysosomes in human fibroblasts produced by serum withdrawal, amino-acid deprivation and confluent conditions

Articles

The pro-urokinase plasminogen-activation system in the presence of serpin-type inhibitors and the urokinase receptor: rescue of activity through reciprocal pro-enzyme activation

Niels BEHRENDT, Karin LIST, Peter A. ANDREASEN, Keld DANØ

Biochem J (2003) 371 (2): 277–287.

https://doi.org/10.1042/bj20021508

Published: 15 April 2003

... ). 27 9 2002 16 1 2003 20 1 2003 20 1 2003 The Biochemical Society, London ©2003 2003 α 2 -antiplasmin cascade system plasminogen activator inhibitor type 1 (PAI-1) proteolysis urokinase-type plasminogen activator receptor (uPAR) Abbreviations used: H- d -Val...

View article titled, The pro-urokinase plasminogen-activation system in the presence of serpin-type inhibitors and the urokinase receptor: rescue of activity through reciprocal pro-enzyme activation

Articles

Identification, functional expression and enzymic analysis of two distinct CaaX proteases from Caenorhabditis elegans

Juan CADIÑANOS, Walter K. SCHMIDT, Antonio FUEYO, Ignacio VARELA, Carlos LÓPEZ-OTÍN, José M.P. FREIJE

Biochem J (2003) 370 (3): 1047–1054.

https://doi.org/10.1042/bj20021514

Published: 15 March 2003

..., prenylation, proteolysis, Ras. cleaves the precursor of the yeast a-factor mating pheromone after the farnesylated cysteine residue and also at a second point close to the N-terminus of the precursor peptide [3 7]. The second gene encodes Rce1p, a protein that seems to be directly involved in the C-terminal...

View article titled, Identification, functional expression and enzymic analysis of two distinct CaaX proteases from Caenorhabditis elegans

Articles

Degradation of human thymidine kinase is dependent on serine-13 phosphorylation: involvement of the SCF-mediated pathway

Po-Yuan KE, Che-Chuan YANG, I-Chun TSAI, Zee-Fen CHANG

Biochem J (2003) 370 (1): 265–273.

https://doi.org/10.1042/bj20021335

Published: 15 February 2003

.... Given the facts that cell-cycle-dependent proteolysis is highly conserved in all eukaryotes and yeast cells are an excellent model system for protein-degradation study, here we report on the use of Saccharomyces cerevisiae and Schizosaccharomyces pombe to investigate the degradation signal and mechanism...

View article titled, Degradation of human thymidine kinase is dependent on serine-13 phosphorylation: involvement of the SCF-mediated pathway

Articles

Structural and functional characterization of tissue factor pathway inhibitor following degradation by matrix metalloproteinase-8

Anna C. CUNNINGHAM, Karen A. HASTY, Jan J. ENGHILD, Alan E. MAST

Biochem J (2002) 367 (2): 451–458.

https://doi.org/10.1042/bj20020696

Published: 15 October 2002

..., at Research Service-151, VA Hospital, 1030 Jefferson Ave., Memphis, TN 38104, U.S.A. (e-mail [email protected] ). 2 5 2002 18 6 2002 12 7 2002 12 7 2002 The Biochemical Society, London ©2002 2002 blood coagulation neutrophil collagenase proteolysis...

View article titled, Structural and functional characterization of tissue factor pathway inhibitor following degradation by matrix metalloproteinase-8

Articles

Substrate specificity of the metalloproteinase pregnancy-associated plasma protein-A (PAPP-A) assessed by mutagenesis and analysis of synthetic peptides: substrate residues distant from the scissile bond are critical for proteolysis

Lisbeth S. LAURSEN, Michael T. OVERGAARD, Claus G. NIELSEN, Henning B. BOLDT, Kathrin H. HOPMANN, Cheryl A. CONOVER, Lars SOTTRUP-JENSEN, Linda C. GIUDICE, Claus OXVIG

Biochem J (2002) 367 (1): 31–40.

https://doi.org/10.1042/bj20020831

Published: 01 October 2002

... properties of the pappalysins. Further, proteinase-resistant variants of IGFBP-4 and −5, presented here, will be useful tools for the study of proteolysis in cell-based systems, and our finding that a synthetic peptide can be cleaved by PAPP-A provides the basis for development of quantitative assays...

View article titled, Substrate specificity of the metalloproteinase pregnancy-associated plasma protein-A (PAPP-A) assessed by mutagenesis and analysis of synthetic peptides: substrate residues distant from the scissile bond are critical for proteolysis

Articles

Cathepsin B expression and down-regulation by gene silencing and antisense DNA in human chondrocytes

Roman ZWICKY, Kathrin MÜNTENER, Mary B. GOLDRING, Antonio BAICI

Biochem J (2002) 367 (1): 209–217.

https://doi.org/10.1042/bj20020210

Published: 01 October 2002

...Roman ZWICKY; Kathrin MÜNTENER; Mary B. GOLDRING; Antonio BAICI Cathepsin B, a marker of the dedifferentiated chondrocyte phenotype, contributes to cartilage destruction in osteoarthritis and pathological proteolysis in rheumatoid arthritis and cancer. In search of possible means for neutralizing...

View article titled, Cathepsin B expression and down-regulation by gene silencing and antisense DNA in human chondrocytes

Articles

A novel form of the RelA nuclear factor κB subunit is induced by and forms a complex with the proto-oncogene c-Myc

Neil R. CHAPMAN, Gill A. WEBSTER, Peter J. GILLESPIE, Brian J. WILSON, Dorothy H. CROUCH, Neil D. PERKINS

Biochem J (2002) 366 (2): 459–469.

https://doi.org/10.1042/bj20020444

Published: 01 September 2002

... be addressed (e-mail [email protected] ). 22 3 2002 22 5 2002 23 5 2002 23 5 2002 The Biochemical Society, London ©2002 2002 cancer proteolysis Ras repression transcription Abbreviations used: DTT, dithiothreitol; EMSA, electrophoretic mobility-shift assay...

View article titled, A novel form of the RelA nuclear factor κB subunit is induced by and forms a complex with the proto-oncogene c-Myc

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