...Sarah E. Garnish; Christopher R. Horne; Yanxiang Meng; Samuel N. Young; Annette V. Jacobsen; Joanne M. Hildebrand; James M. Murphy Necroptosis is a lytic and pro-inflammatory form of programmed cell death executed by the terminal effector, the MLKL (mixed lineage kinase domain-like) pseudokinase...

...Dominic P. Byrne; Safal Shrestha; Leonard A. Daly; Vanessa Marensi; Krithika Ramakrishnan; Claire E. Eyers; Natarajan Kannan; Patrick A. Eyers Pseudokinases, so named because they lack one or more conserved canonical amino acids that define their catalytically active relatives, have evolved...

...Jinle Tang; Yingjie Wang; Huan Zhou; Yuxin Ye; Manisha Talukdar; Ziyang Fu; Zhihong Liu; Jihui Li; Dante Neculai; Jiali Gao; Hao Huang The pseudokinase (PK) RNase L is a functional ribonuclease and plays important roles in human innate immunity. The ribonuclease activity of RNase L can be regulated...

...Fiona P. Bailey; Dominic P. Byrne; Krishnadev Oruganty; Claire E. Eyers; Christopher J. Novotny; Kevan M. Shokat; Natarajan Kannan; Patrick A. Eyers We report that Tribbles 2 (TRB2), a pseudokinase linked to cancer, binds ATP and autophosphorylates in a metal-independent manner. This activity...

... (the kinome) encodes approximately 50 ‘pseudokinases’, which were initially predicted to be incapable of dynamic cell signalling when compared with canonical enzymatically active kinases. This assumption was supported by bioinformatics, which showed that amino acid changes at one or more key loci, making up...

... diseases, including haemopoietic malignancies, immunodeficiency and inflammatory diseases. At the molecular level, recent studies have greatly advanced our knowledge of the structures and organization of the component FERM (4.1/ezrin/radixin/moesin)-SH2 (Src homology 2), pseudokinase and kinase domains...

... receptor activation by cognate haemopoietic cytokines, including erythropoietin and thrombopoietin. The pseudokinase domain (JH2) of JAK2 negatively regulates the catalytic activity of the adjacent tyrosine kinase domain (JH1) and mutations within the pseudokinase domain underlie human myeloproliferative...

...James M. Murphy; Isabelle S. Lucet; Joanne M. Hildebrand; Maria C. Tanzer; Samuel N. Young; Pooja Sharma; Guillaume Lessene; Warren S. Alexander; Jeffrey J. Babon; John Silke; Peter E. Czabotar The pseudokinase MLKL (mixed lineage kinase domain-like) was identified recently as an essential...

...; Christine L. Gee; Ling Liu; Roger J. Daly; Gerard Manning; Jeffrey J. Babon; Isabelle S. Lucet Protein kinase-like domains that lack conserved residues known to catalyse phosphoryl transfer, termed pseudokinases, have emerged as important signalling domains across all kingdoms of life. Although predicted...

... and re-uses a stable modular entity and fold that has evolved to participate in protein–protein interactions in a similar fashion to the catalytically inert pseudokinases. The gene fragments encoding EssB-N and EssB-C were expressed and the recombinant proteins purified using the same protocols...