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Keywords: purification
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Articles
Biochem J (2020) 477 (3): 601–614.
Published: 06 February 2020
... Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 biliverdin biliverdin reductase characterization heme heme oxygenase purification Cellular levels of pro-oxidant free heme are regulated by a two-step catabolic process that initially generates...
Includes: Supplementary data
Articles
Biochem J (2014) 461 (2): 269–278.
Published: 26 June 2014
... are involved in numerous diseases, but study of the structure and function of these proteins is often hampered by their large size and membrane location. Membrane protein purification usually utilizes detergents to solubilize the protein from the membrane, effectively removing it from its native lipid...
Includes: Supplementary data
Articles
Biochem J (2009) 418 (2): 421–429.
Published: 11 February 2009
... phosphatidylethanolamine (PE) phosphatidylserine synthase purification PS (phosphatidylserine) is an essential phospholipid for the growth of mammalian cells [ 1 , 2 ], forming 3–10% of the total phospholipids in various mammalian tissues and cultured cells. PS synthesis in mammalian cells occurs through the...
Articles
Biochem J (2008) 413 (3): 545–552.
Published: 15 July 2008
... that they share 67% identity and that all of the 90 cysteine residues are conserved. The purification and identification strategy used for analysis of human salivary mucins was employed for the analysis of horse saliva. The CsCl/4 M GdmCl density-gradient fractionation of horse saliva is shown...
Articles
Biochem J (2006) 396 (3): 449–460.
Published: 29 May 2006
... As in our previous purification study of ClC-2 [ 17 ], we expressed the protein (engineered to possess a polyhistidine tag at its N-terminus) in Sf9 insect cells using the baculovirus system. High levels of ClC-2 expression were achieved and ClC-2 could be purified after detergent solubilization from...
Includes: Supplementary data
Articles
Biochem J (2006) 395 (2): 295–301.
Published: 28 March 2006
... 0.1–2 mM. 1 To whom correspondence should be addressed (email chiara.ciaccio@uniroma2.it ). 24 8 2005 2 12 2005 6 1 2006 6 1 2006 The Biochemical Society, London 2006 eosinophil peroxidase (EPO) glycosylation Pichia pastoris purification thiocyanate...
Articles
Biochem J (2004) 383 (1): 111–119.
Published: 24 September 2004
... cells using the Tet-On inducible expression system and one-step anti-haemagglutinin immunoaffinity purification. The procedure yields 10–20 μg of pure connexin protein from 2.5×10 8 HeLa cells. The purified channels are shown to be useful for in vitro permeability analysis using well established...
Articles
Biochem J (2004) 379 (2): 263–272.
Published: 15 April 2004
... studies is the lack of accurate three-dimensional structural models of these molecules. The CC-chemokine receptor D6 is expressed at exceptionally high levels in heterologous transfectants. Here we report the purification and biochemical characterization of milligram quantities of D6 protein from...
Articles
Biochem J (2003) 376 (1): 261–268.
Published: 15 November 2003
... from rabbit serum [Draganov, Stetson, Watson, Billecke and La Du (2000) J. Biol. Chem. 275 , 33435–33442] and liver [Ozols (1999) Biochem. J. 338 , 265–275]. In the present study, we have identified the presence of PON3 in rat liver microsomes and a method for the purification to homogeneity is...