...-linked, α-ENaC reporter gene construct (pGL3-KR1) expressed in H441 airway epithelial cells. Dexamethasone evoked a concentration-dependent (EC 50 ∼4 μM) increase in transcriptional activity dependent upon a glucocorticoid response element in the α-ENaC sequence. Although dexamethasone also activated...

... deaminases and both exhibit RNA-binding activity towards AU-rich templates. In order to study their temporal and spatial expression patterns in the worm, reporter gene constructs were made using approx. 2kb of upstream sequence. Transfection of C. elegans revealed that both genes localized to the cells...

... FAS gene transcription by Ang II. We demonstrate that Ang II increased luciferase activity by 3-fold in 3T3-L1 adipocytes transfected with fusion constructs linking the FAS promoter to the luciferase reporter gene. Interestingly, we mapped the Ang II regulatory sequences to the insulin-responsive...