... (ECAR), ATP-linked OCR and maximum mt capacity, reflecting lower ATP demand and mt respiration. Cellular respiratory state apparent was unchanged by serum deprivation. 4-OHT reduced OCR independent of serum status. Despite having a higher mt DNA/nuclear DNA ratio than MCF-7 cells, T47D cells have...

..., mostly the cytoskeleton, to study the whole mitochondrial population in the cell in their natural surroundings and it is increasingly being used in both experimental and clinical studies. The functional parameters (affinity for ADP in regulation of respiration) of mitochondria in permeabilized myocytes...

... (Sigma) at 4°C overnight. Proteins were separated by SDS/PAGE (12% gel), and results were visualized using Pro-Q Diamond gel stain (Molecular Probes), autoradiography, silver staining and anti-GST tag immunodetection. coenzyme Q mitochondrion phosphorylation respiration yeast Coenzyme Q...

... more information about the ability to make ATP than do measurements of intermediates and potentials. For isolated mitochondria, the best assay is mitochondrial respiratory control: the increase in respiration rate in response to ADP. For intact cells, the best assay is the equivalent measurement...

... by subtracting electrode responses observed in the absence of Q 1 . 1 Present address: MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U.K. alcohol dehydrogenase mitochondrion modular kinetics NADH dehydrogenase respiration Schizosaccharomyces pombe ubiquinone pool...

...Eveline HUTTER; Kathrin RENNER; Gerald PFISTER; Petra STÖCKL; Pidder JANSEN-DÜRR; Erich GNAIGER Limitation of lifespan in replicative senescence is related to oxidative stress, which is probably both the cause and consequence of impaired mitochondrial respiratory function. The respiration...

... the horse protein in activating respiration of rat liver mitoplasts deficient in endogenous cytochrome c as well as in inhibition of H 2 O 2 production by the initial segment of the respiratory chain of intact rat heart mitochondria. The major role in the difference between the horse and yeast proteins...

... damage and the inhibition of respiration [3]. Thus the NO concentration is critical in determining whether it acts as a physiological signal or patho- logical agent. Tissue NO concentrations reflect the balance between the rates of NO synthesis and breakdown. To date, much has been learnt about...

... increase in [Mg 2+ ] occurs in both chloroplasts and mitochondria, which may be of importance for the control of key regulatory enzymes (e.g. mitochondrial malic enzyme and pyruvate dehydrogenase complex) and for processes connected with light-enhanced dark respiration. A rationale is presented...

... respiration with sodium azide on the activity of histatin 5 and on that of the variant peptides were compared. This caused complete protection of the cells against histatin 5, while the activity of dhvar4 was hardly affected and that of dhvar5 was affected only intermediately. EXPERIMENTAL PROCEDURES...

... wastage is very limited. 20 10 2000 3 1 2001 25 1 2001 The Biochemical Society, London © 2001 2001 2,4-dinitrophenol hepatocytes octanoate proline respiration Biochem. J. (2001) 355, 231 235 (Printed in Great Britain) 231 The mitochondrial consequences of uncoupling...

... be addressed (e-mail [email protected] ). 2 7 2000 3 10 2000 7 11 2000 The Biochemical Society, London © 2001 2001 metabolic rate oxidative phosphorylation reactive oxygen species respiration thermogenesis Biochem. J. (2001) 353, 369 375 (Printed in Great Britain) 369...

... potential kinetics during the phosphorylation cycle in isolated mitochondria indicated that the absence of cardiolipin causes only a moderate deficiency of mitochondrial energy-transforming machinery at 25 °C. However, at 40 °C, respiration was completely uncoupled from phosphorylation for the mutant...