... as detected using 31 P-NMR, enzymatic determinations and agarose gel electrophoresis. Poly P was localized to granules randomly distributed in the sea urchin eggs, as shown by labelling with the poly-P-binding domain of Escherichia coli exopolyphosphatase. These granules were enriched using iodixanol...

...Anthony J. Morgan; Antony Galione NAADP (nicotinic acid–adenine dinucleotide phosphate)-induced Ca 2+ release has been proposed to occur selectively from acidic stores in several cell types, including sea urchin eggs. Using fluorescence measurements, we have investigated whether NAADP-induced Ca 2...

... regulates the target protein. We report in the present paper that NAADP receptors from sea urchin eggs undergo an unusual stabilization process that appears to be dependent upon the time during which receptors are exposed to their ligand. We demonstrate that receptors ‘tagged’ with NAADP for short periods...

...Dev CHURAMANI; Elizabeth A. CARREY; George D. DICKINSON; Sandip PATEL Nicotinic acid–adenine dinucleotide phosphate (NAADP) is fast emerging as a new intracellular Ca 2+ -mobilizing messenger. In sea urchin egg homogenates, binding of NAADP to its receptor is not readily reversible; hence, prior...

...Sandip PATEL; Grant C. CHURCHILL; Antony GALIONE Nicotinic acidŐadenine dinucleotide phosphate (NAADP) is a novel and potent Ca 2+ -mobilizing agent in sea urchin eggs and other cell types. Little is known, however, concerning the properties of the putative intracellular NAADP receptor...