... of secondary structure predictions suggest the presence of two α-helices within the N-terminal region of the MTS. Results from deletion analyses indicate that individual helices have limited ability to direct protein import and matrix localization, but that there is a synergistic interaction when both helices...

... for the pathogenicity associated with AS oligomers. amyloidogenesis amyloid oligomer Parkinson's disease secondary structure structure–toxicity relationship α-synuclein In the so-called protein deposition diseases, specific proteins or peptides fail to adopt or remain in their native functional...

..., Australia. 2 To whom correspondence should be addressed (email [email protected] ). 5 5 2006 17 8 2006 8 9 2006 8 9 2006 The Biochemical Society, London 2007 CD spectroscopy folding intermediate prion protein (PrP) secondary structure translational diffusion...

...; indeed, a handful of co-repressors may be sufficient to accurately fulfil the task in hand. AF1 transactivation domain allosteric regulation protein–nucleic acid interaction protein–protein interaction post-translational modification secondary structure steroid receptor The AF1 domain...

... data, and the corrected ellipticities were converted into mean residue ellipticities using average molecular masses per residue of 118.0 (LytC) and 115.8 (Cpl-1). Secondary-structural content was estimated by deconvolution of the experimental curves using the CONTIN (16 protein spectra data set...

...) was generated as described previously [ 26 ]. Recombinant PrP proteins were verified by MS to confirm the correct protein sequence and the presence of a disulphide bond. blood cell cell-surface prion disease prion-related protein (PrP) secondary structure transmissible spongiform encephalopathies...

...Antonino NATALELLO; Diletta AMI; Stefania BROCCA; Marina LOTTI; Silvia M. DOGLIA The secondary structure of lipase 1 from Candida rugosa , a model system for large monomeric enzymes, has been studied by FTIR (Fourier-transform infrared) spectroscopy in water and 2 H 2 O. The secondary structure...

... were verified by MS to confirm the correct protein sequence and the presence of a disulphide bond. epitope polymorphism PrP C ruminant secondary structure transmissible spongiform encephalopathy Prion diseases, such as scrapie in sheep, bovine spongiform encephalopathy in cattle...

...-dodecyl-β- d -maltoside-solubilized UCP1–His 6 retained its PN (purine nucleotide)-binding capacity. The far-UV CD spectrum of the functional protein clearly indicated the predominance of α-helices in the UCP1 secondary structure. The UCP1 secondary structure exhibited an α-helical degree of approx. 68...

...-mail [email protected] ). 27 5 2003 16 6 2003 18 6 2003 18 6 2003 The Biochemical Society, London ©2003 2003 amphipathicity Candida albicans Cryptococcus neoformans MUC7 12-mer secondary structure Abbreviations used: CAMP, cationic antimicrobial peptide...

.... Previously it has been shown that transmembrane domain 4 (TM4) is crucial for the function of this protein. Here we provide the first direct experimental evidence for secondary-structural features and membrane insertions of a 24-residue peptide, corresponding to TM4 of DMT1 (DMTI-TM4), in various membrane...

... secondary structure Biochem. J. (2000) 352, 117 124 (Printed in Great Britain) 117 In vitro membrane-inserted conformation of the cytochrome b5 tail Michael R. HANLON*, Rukhsana R. BEGUM‹, Richard J. NEWBOLD‹, David WHITFORD‹ and B. A. WALLACE*1 *Department of Crystallography, Birkbeck College...

... © 2000 2000 amphipathic helix hypochlorous acid primary structure secondary structure Biochem. J. (2000) 346, 345 354 (Printed in Great Britain) 345 Reagent or myeloperoxidase-generated hypochlorite affects discrete regions in lipid-free and lipid-associated human apolipoprotein A-I...

... 7 1999 The Biochemical Society, London © 1999 1999 CD analysis folding pathway insulin-like growth factor I secondary structure Biochem. J. (1999) 343, 139 144 (Printed in Great Britain) 139 Intra-A chain disulphide bond forms first during insulin precursor folding Ying YUAN, Zhao...

... cells. In addition, MS of native H,K-ATPase tryptic peptides identified cytoplasmically oriented HKα residues. The results indicated cytoplasmic exposure of Leu 844 and Phe 996 , and luminal exposure of Pro 898 , leading to a revised secondary structure model of the C-terminal third of HKα. 1...