... suppressed it by converting the RAGE isoform from a membrane-bound into a soluble form. This shedding is the result of ectodomain cleavage of mRAGE (membrane-bound RAGE) by MMP9 (matrix metalloproteinase 9). Furthermore, forskolin significantly inhibited peritoneal macrophage accumulation in a mouse S100B...

.... In the present paper, we report that thrombin mediates shedding of an N-terminal TEM5 fragment of 60 kDa (termed N60) containing the RGD motif in an open conformation. Thrombin directly cleaved rsTEM5 (recombinant soluble TEM5) 5 and 34 residues downstream of the RGD motif, resulting in formation of N60 and its...

... [email protected] ). 3 2 2010 17 3 2010 29 3 2010 29 3 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 disulfide bond pattern metalloproteinase protein conformation protein disulfide isomerase (PDI) redox regulation shedding...

... known as TACE [TNFα (tumour necrosis factor α)-converting enzyme]}, which is involved in processing pro-TNFα and in the activation of ligands for the EGFR [EGF (epidermal growth factor) receptor], as well as cleavage of diverse cell-surface receptors and adhesion molecules. ADAM-mediated shedding...

... as ‘shedding’. Among the major targets of ADAM10 are Notch, EphrinA2 and CD44. In many cell-based studies of shedding, the activity of ADAM10 appears to overlap with that of ADAM17, which has a similar active-site topology relative to the other proteolytically active ADAMs. The tissue inhibitors...

... with haematoxylin and eosin, and were counted under a light microscope in five defined high-power fields (×200 magnification). a disintegrin and metalloproteinase (ADAM) CD44 cell-surface adhesion molecule epidermal growth factor (EGF) Rac GTPase shedding CD44 is a major cell-surface adhesion...

...Marja-Riitta Väisänen; Timo Väisänen; Hongmin Tu; Päivi Pirilä; Raija Sormunen; Taina Pihlajaniemi Type XIII collagen is a transmembrane collagen, which is known to exist also as a soluble variant due to ectodomain shedding. Earlier studies with the recombinant ectodomain have shown it to interact...

...Marta TOTH; Pamela OSENKOWSKI; Dusan HESEK; Stephen BROWN; Samy MEROUEH; Wael SAKR; Shahriar MOBASHERY; Rafael FRIDMAN MT1-MMP (membrane type 1 matrix metalloproteinase) is a membrane-anchored MMP that can be shed to the extracellular milieu. In the present study we report the primary structure...

... in the pericellular matrix, as the ectodomain is released from the plasma membrane by proteolytic cleavage. Analysis with various protease inhibitors led to confirmation of the furin family of proprotein convertases as the protease group responsible for the shedding of the ectodomain, cleaving at a site conforming...

... enzyme following cleavage mediated by an unidentified protease(s), referred to as ACE secretase, in a process known as ‘shedding'. The effects of monoclonal antibodies (mAbs) to eight different epitopes on the N-terminal domain of ACE on shedding was investigated using Chinese hamster ovary cells (CHO...

... endocytosis endothelium mevastatin NF-κB shedding Abbreviations used: DMEM, Dulbecco's modified Eagle's medium; EMSA, electrophoretic mobility shift assay; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HMG-CoA, hydroxymethylglutaryl-CoA; HRP, horseradish peroxidase; IL-1β, interleukin-1β; NF...

...Elena DÍAZ-RODRÍGUEZ; Azucena ESPARÍS-OGANDO; Juan Carlos MONTERO; Laura YUSTE; Atanasio PANDIELLA The ectodomain of several membrane-bound proteins can be shed by proteolytic cleavage. The activity of the proteases involved in shedding is highly regulated by several intracellular second messenger...