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Keywords: site-directed mutagenesis
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Biochem J (2023) 480 (4): 259–281.
Published: 23 February 2023
... 2 2 2023 © 2023 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2023 bacterial metabolism enzyme kinetics molecular docking protein crystallography site-directed mutagenesis structure–function relationships In agreement with the well...
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Biochem J (2021) 478 (14): 2871–2887.
Published: 23 July 2021
... 5 2021 18 6 2021 29 6 2021 30 6 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 electron transfer Geobacter multiheme cytochrome c NMR spectroscopy site-directed mutagenesis Geobacter bacteria...
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Biochem J (2021) 478 (10): 1921–1941.
Published: 24 May 2021
.... A multifunctional plant degradation enzyme was constructed with three functional roles of absorption, degradation, and mineralization using amino acid sequence recombination and site-directed mutagenesis to modify the template of plant degradation enzyme. Finally, using the Taguchi experimental design-assisted...
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Biochem J (2020) 477 (7): 1241–1259.
Published: 09 April 2020
... inhibition kinetics metalloproteases protein crystallography site-directed mutagenesis Angiotensin-converting enzyme (ACE, EC 3.4.15.1) is a zinc metalloprotease which has traditionally been known for its role in blood pressure regulation via the renin–angiotensin–aldosterone system (RAAS). During...
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Biochem J (2019) 476 (24): 3835–3847.
Published: 23 December 2019
... as the template for the introduction of single amino acid substitution generated by PCR. The amino acid residues, C240, H135, R249 and Y118 were mutated to encode Ala at these positions using appropriate primers listed in ( Table 2 ) using Q5 site-directed mutagenesis kit (NEB). After PCR, the mutant plasmids...
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Biochem J (2019) 476 (21): 3333–3353.
Published: 15 November 2019
... phosphodiesterase second messenger site-directed mutagenesis Cyclic dinucleotides (CDNs) have been implicated in modulating varieties of cellular activities in bacteria that include motility [ 1 ], virulence [ 2 ], biofilm formation and dispersal [ 3 , 4 ] and exopolysaccharide synthesis [ 5 ]. c-di-GMP...
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Biochem J (2018) 475 (23): 3887–3901.
Published: 12 December 2018
...). Published by Portland Press Limited on behalf of the Biochemical Society 2018 homoserine malate dehydrogenase protein engineering site-directed mutagenesis synthetic biology A central challenge in the creation of a resource-efficient and sustainable bioeconomy lies in the reduction in our...
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Biochem J (2018) 475 (23): 3861–3873.
Published: 10 December 2018
... residues based on a site-directed mutagenesis study [ 15 ]. A third catalytic residue, asparagine was also identified based on structure modeling and mutational studies [ 11 ]. Based on these results, a papain-like catalytic triad consisting of the amino acid residues, Asn, His, and Cys, was proposed...
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Biochem J (2017) 474 (11): 1807–1821.
Published: 16 May 2017
... Society 2017 oligopeptide transporter family Saccharomyces cerevisiae site-directed mutagenesis transmembrane proteins transport Western blot analysis using crude cell preparation was carried out as described previously [ 9 , 25 ]. To quantify the protein expression levels in different...
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Biochem J (2016) 473 (21): 4011–4026.
Published: 27 October 2016
... 2016 © 2016 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2016 cold adaptation crystallography ice growth inhibition molecular docking site-directed mutagenesis thermal hysteresis Organisms that are exposed to subzero temperatures...
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Biochem J (2016) 473 (15): 2369–2382.
Published: 28 July 2016
...-directed mutagenesis substrate specificity transmembrane domain Glutathione (γ-glutamyl- L -cysteinyl-glycine) is an essential metabolite in all unicellular and multicellular eukaryotes, barring a few amitochondrial organisms [ 1 , 2 ]. Glutathione defends against oxidative stress...
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Biochem J (2016) 473 (8): 1085–1095.
Published: 08 April 2016
... resin COSMOGEL His-Accept was purchased from Nacalai Tesque. Sephacryl S-100 HR was from GE Healthcare. All other reagents were of analytical grade. CBM32 chitosan crystal structure NMR spectroscopy isothermal titration calorimetry site-directed mutagenesis Interactions between soil...
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Biochem J (2012) 445 (3): 313–322.
Published: 13 July 2012
... superfamily (MFS) transporter MDR1 (multidrug resistance 1) site-directed mutagenesis The overexpression of the drug efflux pumps encoding the genes CDR ( Candida drug resistance) 1 and CDR2 of the ABC (ATP-binding cassette) family of transporters and MDR1 (multidrug resistance 1...
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Biochem J (2012) 444 (3): 437–443.
Published: 29 May 2012
... ′-diacetylchitobiosyl fluoride [α-(GlcNAc) 2 -F] site-directed mutagenesis Chitin oligosaccharides (GlcNAc) n [a β-1,4-linked polysaccharide of GlcNAc ( N -acetylglucosamine) with a polymerization degree of n ] have been drawing attention because of their presumed biological functions, such as inhibition...
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Biochem J (2012) 442 (2): 357–368.
Published: 13 February 2012
... cerevisiae site-directed mutagenesis The capacity of a growing number of proteins to ‘moonlight’ – that is, to develop several functions within a single polypeptide chain that often are apparently unrelated – represents a way to expand the functional capabilities of an organism in spite...
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Biochem J (2011) 435 (2): 411–420.
Published: 29 March 2011
... 2011 aminopeptidase antigen kinetics peptide specificity site-directed mutagenesis substrate library © The Authors Journal compilation © 2011 Biochemical Society 2011 Cytotoxic T-lymphocytes identify infected or transformed cells by recognizing small antigenic peptides bound...
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Biochem J (2010) 432 (3): 445–454.
Published: 25 November 2010
... pyridoxal 5-phosphate synthase Saccharomyces cerevisiae site-directed mutagenesis The active form of vitamin B6, PLP (pyridoxal 5-phosphate), is an essential cofactor for numerous metabolic enzymes. PLP also has an important role in amino acid and carbohydrate metabolism [ 1 , 2 ]. To date, two...
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Biochem J (2010) 432 (3): 565–573.
Published: 25 November 2010
... structure hydrogen bonding OpdA organophosphate-degrading enzyme organophosphate pesticide site-directed mutagenesis The intensive application of pesticides has facilitated the development and expansion of agriculture globally. Despite considerable evidence of environmental toxicity, the use...
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Biochem J (2010) 430 (3): 539–549.
Published: 27 August 2010
... ]. © The Authors Journal compilation © 2010 Biochemical Society 2010 autophosphorylation bacterium enzyme kinetics NTP synthesis nucleoside diphosphate kinase phosphotransfer activity site-directed mutagenesis NDK is well-known as a phosphoprotein and autophosphorylation...
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Biochem J (2010) 426 (3): 337–344.
Published: 24 February 2010
... 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 archaeon argonaute double-stranded RNA (dsRNA) ribonuclease H RNA–DNA duplex site-directed mutagenesis RNase H (ribonuclease H) is a ubiquitous enzyme found in all three kingdoms of the tree of life: archaea...
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Biochem J (2008) 416 (1): 109–116.
Published: 28 October 2008
... specificity toward synthetic substrates, its substrate specificity toward natural peptides is rather broad. Because of the pathophysiological significance of ERAP-1, it is important to elucidate the molecular basis of its enzymatic action. In the present study we used site-directed mutagenesis to identify...
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Biochem J (2008) 414 (3): 383–390.
Published: 27 August 2008
... isoforms of PI3K (phosphoinositide 3-kinase), yet little has been revealed about the molecular basis for the observed selectivities. Using site-directed mutagenesis, we have investigated one of the areas postulated as being critical to the observed selectivity. The residues Thr 886 and Lys 890 of the PI3Kγ...
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Biochem J (2008) 411 (3): 467–473.
Published: 14 April 2008
...Cheng-Chin Kuo; Kuan-Yu Lin; Yau-Jung Hsu; Shu-Yu Lin; Yu-Tsen Lin; Gu-Gang Chang; Wei-Yuan Chou The role of general acid–base catalysis in the enzymatic mechanism of NADP + -dependent malic enzyme was examined by detailed steady-state kinetic studies through site-directed mutagenesis of the Tyr 91...
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Biochem J (2008) 411 (3): 553–561.
Published: 14 April 2008
... © 2008 Biochemical Society 2008 acetylation high mobility group (HMG) box NMR spectroscopy non-histone protein site-directed mutagenesis Protein acetylation plays a critical role in the control of gene transcription. This is effected through modulation of chromatin packaging...
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Biochem J (2008) 411 (1): 181–190.
Published: 13 March 2008
... (glutaminyl-peptide cyclotransferase QC) pyroglutamate (pGlu) hydrogen-bond network proton transfer site-directed mutagenesis synchrotron X-ray crystallography QCs (glutaminyl cyclases; glutaminyl-peptide cyclotransferases, EC 2.3.2.5) catalyse the conversion of protein N-terminal glutamine...
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Biochem J (2008) 410 (3): 543–553.
Published: 27 February 2008
... the enzyme from DEPC inactivation. Site-directed mutagenesis experiments in which the 15 histidine residues in the enzyme were replaced individually with alanine corroborated the chemical modification studies and established the importance of His-335 in the methylase activity. No gross structural differences...
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Biochem J (2007) 405 (2): 299–306.
Published: 27 June 2007
... extracellular matrix metalloproteinase procollagen proteolytic processing site-directed mutagenesis transient cell transfection Amino acid sequence analysis was performed by automated Edman degradation using an Applied Biosystems 473A protein sequencer. For N-terminal sequencing of the TSPN-1 released...
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