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Keywords: site-directed mutagenesis
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Biochem J (2023) 480 (4): 259–281.
Published: 23 February 2023
... 2 2 2023 © 2023 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2023 bacterial metabolism enzyme kinetics molecular docking protein crystallography site-directed mutagenesis structure–function relationships In agreement with the well...
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Biochem J (2021) 478 (14): 2871–2887.
Published: 23 July 2021
... 5 2021 18 6 2021 29 6 2021 30 6 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 electron transfer Geobacter multiheme cytochrome c NMR spectroscopy site-directed mutagenesis Geobacter bacteria...
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Biochem J (2021) 478 (10): 1921–1941.
Published: 24 May 2021
.... A multifunctional plant degradation enzyme was constructed with three functional roles of absorption, degradation, and mineralization using amino acid sequence recombination and site-directed mutagenesis to modify the template of plant degradation enzyme. Finally, using the Taguchi experimental design-assisted...
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Biochem J (2020) 477 (17): 3147–3165.
Published: 04 September 2020
... molecular dynamics NMR relaxation NMR spectroscopy site-directed mutagenesis Screening for up- or down-regulation of expression of distinct genes upon changing a specific parameter is a robust approach to identify key aspects of the cascade leading to a triggered cellular response. When...
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Biochem J (2020) 477 (7): 1241–1259.
Published: 09 April 2020
... inhibition kinetics metalloproteases protein crystallography site-directed mutagenesis Angiotensin-converting enzyme (ACE, EC 3.4.15.1) is a zinc metalloprotease which has traditionally been known for its role in blood pressure regulation via the renin–angiotensin–aldosterone system (RAAS). During...
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Biochem J (2019) 476 (21): 3333–3353.
Published: 15 November 2019
... in the active site, helped us to propose a mechanism to further elucidate the catalytic cycle. c-di-GMP cGAMP crystallography phosphodiesterase second messenger site-directed mutagenesis The gene encoding a putative uncharacterized EAL domain ( Vc EAL) which contains amino acids 1–257...
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Biochem J (2018) 475 (23): 3887–3901.
Published: 12 December 2018
... of the Biochemical Society 2018 homoserine malate dehydrogenase protein engineering site-directed mutagenesis synthetic biology A central challenge in the creation of a resource-efficient and sustainable bioeconomy lies in the reduction in our dependence on fossil resources and a shift in focus...
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Biochem J (2018) 475 (23): 3861–3873.
Published: 10 December 2018
... into a pUC57 vector such that expression was transcriptionally driven by the lacZ promoter [ 4 ]. Using these plasmids, mutations at specific conserved sites were generated with the QuikChange site-directed mutagenesis kit (Agilent Technologies, U.S.A.), following the manufacturer's instructions. Residues...
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Biochem J (2017) 474 (11): 1807–1821.
Published: 16 May 2017
... Saccharomyces cerevisiae site-directed mutagenesis transmembrane proteins transport The electrochemical proton gradient acts as the driving force for the uptake and efflux of many metabolites and ions across the plasma membrane of fungi and plants [ 1 , 2 ]. A large number of transporters...
Includes: Supplementary data
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Biochem J (2016) 473 (21): 4011–4026.
Published: 27 October 2016
... 9 9 2016 © 2016 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2016 cold adaptation crystallography ice growth inhibition molecular docking site-directed mutagenesis thermal hysteresis Organisms that are exposed to subzero...
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Biochem J (2016) 473 (15): 2369–2382.
Published: 28 July 2016
...-directed mutagenesis substrate specificity transmembrane domain Glutathione (γ-glutamyl- L -cysteinyl-glycine) is an essential metabolite in all unicellular and multicellular eukaryotes, barring a few amitochondrial organisms [ 1 , 2 ]. Glutathione defends against oxidative stress...
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Biochem J (2016) 473 (8): 1085–1095.
Published: 08 April 2016
... CBM32 chitosan crystal structure NMR spectroscopy isothermal titration calorimetry site-directed mutagenesis Interactions between soil bacteria and fungal pathogens play important roles in sustainably managing agricultural production from soil [ 1 ]. Fungal cell walls contain several kinds...
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Biochem J (2012) 445 (3): 313–322.
Published: 13 July 2012
... nitrogen base, 0.2% dropout mix and 2% glucose; Difco). For the agar plates, 2.5% (w/v) Bacto agar (Difco) was added to the medium. The site-directed mutagenesis was performed using the QuikChange® site-directed mutagenesis kit (Stratagene) as described previously [ 20 ]. The mutations were...
Includes: Supplementary data
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Biochem J (2012) 444 (3): 437–443.
Published: 29 May 2012
... available commercially. chitin oligosaccharide chitobiose phosphorylase glycosynthase α- N , N ′-diacetylchitobiosyl fluoride [α-(GlcNAc) 2 -F] site-directed mutagenesis Chitin oligosaccharides (GlcNAc) n [a β-1,4-linked polysaccharide of GlcNAc ( N -acetylglucosamine...
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Biochem J (2012) 442 (2): 357–368.
Published: 13 February 2012
... times in total. After sedimentation at 500  g for 10 min at 4°C, the cleared lysate was recovered and the protein concentration was quantified by a Bradford assay. CoA biosynthesis multifunctional protein protein phosphatase regulatory subunit Saccharomyces cerevisiae site-directed...
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Biochem J (2011) 435 (2): 411–420.
Published: 29 March 2011
... from different plates. © The Authors Journal compilation © 2011 Biochemical Society 2011 aminopeptidase antigen kinetics peptide specificity site-directed mutagenesis substrate library Cytotoxic T-lymphocytes identify infected or transformed cells by recognizing small...
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Biochem J (2010) 432 (3): 445–454.
Published: 25 November 2010
... pyridoxal 5-phosphate synthase Saccharomyces cerevisiae site-directed mutagenesis The active form of vitamin B6, PLP (pyridoxal 5-phosphate), is an essential cofactor for numerous metabolic enzymes. PLP also has an important role in amino acid and carbohydrate metabolism [ 1 , 2 ]. To date, two...
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Biochem J (2010) 430 (3): 539–549.
Published: 27 August 2010
... to discriminate between such cations [ 30 ]. © The Authors Journal compilation © 2010 Biochemical Society 2010 autophosphorylation bacterium enzyme kinetics NTP synthesis nucleoside diphosphate kinase phosphotransfer activity site-directed mutagenesis Figure 1 Autophosphorylation...
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Biochem J (2010) 426 (3): 281–292.
Published: 24 February 2010
...://www.BiochemJ.org/bj/426/bj4260281add.htm ), and the PCR products were cloned directly into the bacterial expression vector pGEX6P1 (GE Healthcare). Site-directed mutagenesis was carried out following the QuikChange® site-directed mutagenesis protocol (Stratagene), using the KOD HotStart DNA polymerase...
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Biochem J (2010) 426 (3): 337–344.
Published: 24 February 2010
... 2010 5 1 2010 5 1 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 archaeon argonaute double-stranded RNA (dsRNA) ribonuclease H RNA–DNA duplex site-directed mutagenesis A structural model was constructed using the molecular graphics software...
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Biochem J (2008) 416 (1): 109–116.
Published: 28 October 2008
... specificity toward synthetic substrates, its substrate specificity toward natural peptides is rather broad. Because of the pathophysiological significance of ERAP-1, it is important to elucidate the molecular basis of its enzymatic action. In the present study we used site-directed mutagenesis to identify...
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Biochem J (2008) 414 (3): 383–390.
Published: 27 August 2008
... isoforms of PI3K (phosphoinositide 3-kinase), yet little has been revealed about the molecular basis for the observed selectivities. Using site-directed mutagenesis, we have investigated one of the areas postulated as being critical to the observed selectivity. The residues Thr 886 and Lys 890 of the PI3Kγ...
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Biochem J (2008) 411 (3): 553–561.
Published: 14 April 2008
... are shown. Note that the gels for the wild-type A domain and AB di-domain are reproduced from [ 23 ] with permission. © 2001 Elsevier. acetylation high mobility group (HMG) box NMR spectroscopy non-histone protein site-directed mutagenesis Protein acetylation plays a critical role...
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Biochem J (2008) 411 (3): 467–473.
Published: 14 April 2008
...Cheng-Chin Kuo; Kuan-Yu Lin; Yau-Jung Hsu; Shu-Yu Lin; Yu-Tsen Lin; Gu-Gang Chang; Wei-Yuan Chou The role of general acid–base catalysis in the enzymatic mechanism of NADP + -dependent malic enzyme was examined by detailed steady-state kinetic studies through site-directed mutagenesis of the Tyr 91...
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Biochem J (2008) 411 (1): 181–190.
Published: 13 March 2008
... pathways, such as the participation of Asp 248 and Asp 305 , in the catalysis process, could not be completely ruled out thus far. glutaminyl cyclase (glutaminyl-peptide cyclotransferase QC) pyroglutamate (pGlu) hydrogen-bond network proton transfer site-directed mutagenesis synchrotron X-ray...
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