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Keywords: site-directed mutagenesis
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Articles
Arline Fernández-Silva, Ana L. Juárez-Vázquez, Lilian González-Segura, Javier Andrés Juárez-Díaz, Rosario A. Muñoz-Clares
Journal:
Biochemical Journal
Biochem J (2023) 480 (4): 259–281.
Published: 23 February 2023
... 2 2 2023 © 2023 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2023 bacterial metabolism enzyme kinetics molecular docking protein crystallography site-directed mutagenesis structure–function relationships In agreement with the well...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2021) 478 (14): 2871–2887.
Published: 23 July 2021
... 5 2021 18 6 2021 29 6 2021 30 6 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 electron transfer Geobacter multiheme cytochrome c NMR spectroscopy site-directed mutagenesis Geobacter bacteria...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2021) 478 (10): 1921–1941.
Published: 24 May 2021
.... A multifunctional plant degradation enzyme was constructed with three functional roles of absorption, degradation, and mineralization using amino acid sequence recombination and site-directed mutagenesis to modify the template of plant degradation enzyme. Finally, using the Taguchi experimental design-assisted...
Articles
Michelle C. Miller, Irina V. Nesmelova, Vladimir A. Daragan, Hans Ippel, Malwina Michalak, Aurelio Dregni, Herbert Kaltner, Jürgen Kopitz, Hans-Joachim Gabius, Kevin H. Mayo
Journal:
Biochemical Journal
Biochem J (2020) 477 (17): 3147–3165.
Published: 04 September 2020
... involving inter- and intrasubunit disulfide bridges by gel filtration, ultracentrifugation, site-directed mutagenesis, and peptide mass fingerprinting . J. Mol. Biol. 386 , 366 – 378 10.1016/j.jmb.2008.09.054 83 Miller , M.C. , Ludwig , A.-K. , Wichapong , K. , Kaltner , H...
Includes: Supplementary data
Articles
Yannan Wang, Laurie A. Graham, Zhifu Han, Robert Eves, Audrey K. Gruneberg, Robert L. Campbell, Heqiao Zhang, Peter L. Davies
Journal:
Biochemical Journal
Biochem J (2020) 477 (12): 2179–2192.
Published: 22 June 2020
.... Unexpectedly, close examination of its surface did not reveal any large regions of flat, regularly spaced hydrophobic residues that characterize the ice-binding sites (IBSs) of potent antifreeze proteins from freeze-resistant fish and insects. An IBS was defined by site-directed mutagenesis of residues...
Includes: Multimedia, Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2020) 477 (11): 2095–2114.
Published: 15 June 2020
...Rosario A. Muñoz-Clares; Lilian González-Segura; Javier Andrés Juárez-Díaz; Carlos Mújica-Jiménez Activation of phosphoenolpyruvate carboxylase (PEPC) enzymes by glucose 6-phosphate (G6P) and other phospho-sugars is of major physiological relevance. Previous kinetic, site-directed mutagenesis...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2020) 477 (7): 1241–1259.
Published: 09 April 2020
... inhibition kinetics metalloproteases protein crystallography site-directed mutagenesis Angiotensin-converting enzyme (ACE, EC 3.4.15.1) is a zinc metalloprotease which has traditionally been known for its role in blood pressure regulation via the renin–angiotensin–aldosterone system (RAAS). During...
Articles
Journal:
Biochemical Journal
Biochem J (2019) 476 (24): 3835–3847.
Published: 23 December 2019
... as the template for the introduction of single amino acid substitution generated by PCR. The amino acid residues, C240, H135, R249 and Y118 were mutated to encode Ala at these positions using appropriate primers listed in ( Table 2 ) using Q5 site-directed mutagenesis kit (NEB). After PCR, the mutant plasmids...
Articles
Journal:
Biochemical Journal
Biochem J (2019) 476 (21): 3333–3353.
Published: 15 November 2019
... phosphodiesterase second messenger site-directed mutagenesis Cyclic dinucleotides (CDNs) have been implicated in modulating varieties of cellular activities in bacteria that include motility [ 1 ], virulence [ 2 ], biofilm formation and dispersal [ 3 , 4 ] and exopolysaccharide synthesis [ 5 ]. c-di-GMP...
Includes: Supplementary data
Articles
Yingxue Yang, Esther D. Lenherr, Roland Gromes, Shanshan Wang, Markus Wirtz, Rüdiger Hell, Tanja Peskan-Berghöfer, Klaus Scheffzek, Thomas Rausch
Journal:
Biochemical Journal
Biochem J (2019) 476 (7): 1191–1203.
Published: 15 April 2019
... biosynthesis homo-dimerization redox activation site-directed mutagenesis Glutathione (GSH) and its oxidized form glutathione disulfide (GSSG) are key players of the cellular redox buffer in plants. Total GSH content and changes in the GSH:GSSG ratio both affect its redox potential. To ensure...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2018) 475 (23): 3887–3901.
Published: 12 December 2018
...). Published by Portland Press Limited on behalf of the Biochemical Society 2018 homoserine malate dehydrogenase protein engineering site-directed mutagenesis synthetic biology A central challenge in the creation of a resource-efficient and sustainable bioeconomy lies in the reduction in our...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2018) 475 (23): 3861–3873.
Published: 10 December 2018
... residues based on a site-directed mutagenesis study [ 15 ]. A third catalytic residue, asparagine was also identified based on structure modeling and mutational studies [ 11 ]. Based on these results, a papain-like catalytic triad consisting of the amino acid residues, Asn, His, and Cys, was proposed...
Includes: Supplementary data
Articles
Maike K. Groenewold, Marco Massmig, Stefanie Hebecker, Linna Danne, Zofia Magnowska, Manfred Nimtz, Franz Narberhaus, Dieter Jahn, Dirk W. Heinz, Lothar Jänsch, Jürgen Moser
Journal:
Biochemical Journal
Biochem J (2018) 475 (11): 1885–1907.
Published: 06 June 2018
... of phospholipid metabolism. Structure-guided site-directed mutagenesis was used to explore the proposed ligand-binding cavity of PA3911. Protein variants of Leu 56 , Leu 58 , Val 69 and Leu 114 were shown to impair PA interaction. A comparative shotgun lipidomics approach demonstrated a multifaceted response of P...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2017) 474 (11): 1807–1821.
Published: 16 May 2017
... Society 2017 oligopeptide transporter family Saccharomyces cerevisiae site-directed mutagenesis transmembrane proteins transport Western blot analysis using crude cell preparation was carried out as described previously [ 9 , 25 ]. To quantify the protein expression levels in different...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2016) 473 (21): 4011–4026.
Published: 27 October 2016
... 2016 © 2016 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2016 cold adaptation crystallography ice growth inhibition molecular docking site-directed mutagenesis thermal hysteresis Organisms that are exposed to subzero temperatures...
Articles
Mohammad Zulkifli, Shambhu Yadav, Anil Thakur, Shiffalli Singla, Monika Sharma, Anand Kumar Bachhawat
Journal:
Biochemical Journal
Biochem J (2016) 473 (15): 2369–2382.
Published: 28 July 2016
...-directed mutagenesis substrate specificity transmembrane domain Glutathione (γ-glutamyl- L -cysteinyl-glycine) is an essential metabolite in all unicellular and multicellular eukaryotes, barring a few amitochondrial organisms [ 1 , 2 ]. Glutathione defends against oxidative stress...
Includes: Supplementary data
Articles
Shoko Shinya, Shigenori Nishimura, Yoshihito Kitaoku, Tomoyuki Numata, Hisashi Kimoto, Hideo Kusaoke, Takayuki Ohnuma, Tamo Fukamizo
Journal:
Biochemical Journal
Biochem J (2016) 473 (8): 1085–1095.
Published: 08 April 2016
... resin COSMOGEL His-Accept was purchased from Nacalai Tesque. Sephacryl S-100 HR was from GE Healthcare. All other reagents were of analytical grade. CBM32 chitosan crystal structure NMR spectroscopy isothermal titration calorimetry site-directed mutagenesis Interactions between soil...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2015) 471 (2): 187–198.
Published: 02 October 2015
... phosphatase activity on Cg Pmu2 by creating fusions of sections of CgPMU2 with CgPMU1 , a paralogue with little broad range phosphatase activity. We used site-directed mutagenesis on various fusions to sequentially convert Cg Pmu1 to Cg Pmu2. Based on molecular modelling of the Pmu proteins on to a histidine...
Articles
Dolores Linde, Rebecca Pogni, Marina Cañellas, Fátima Lucas, Victor Guallar, Maria Camilla Baratto, Adalgisa Sinicropi, Verónica Sáez-Jiménez, Cristina Coscolín, Antonio Romero, Francisco Javier Medrano, Francisco J. Ruiz-Dueñas, Angel T. Martínez
Journal:
Biochemical Journal
Biochem J (2015) 466 (2): 253–262.
Published: 20 February 2015
... electron transfer from surface tryptophan residues forming radicals appears as a general mechanism for peroxidase oxidation of bulky substrates. catalytic protein radical dye-decolorizing peroxidase EPR spectroscopy molecular docking QM/MM site-directed mutagenesis DyPs (dye-decolorizing...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2014) 461 (1): 75–85.
Published: 13 June 2014
... Gα proteins of soya bean are functionally significant as observed by in vivo complementation of yeast gpa1 mutant in the pheromone-response pathway. heterotrimeric G-protein plant G-protein site-directed mutagenesis GmGα protein soya bean G-protein activity yeast complementation...
Articles
Guillaume Née, Magali Aumont-Nicaise, Mirko Zaffagnini, Sylvie Nessler, Marielle Valerio-Lepiniec, Emmanuelle Issakidis-Bourguet
Journal:
Biochemical Journal
Biochem J (2014) 457 (1): 117–125.
Published: 10 December 2013
... binding to its active site. dithiol/disulfide interchange glucose-6-phosphate dehydrogenase redox regulation site-directed mutagenesis thioredoxin In photosynthetic cells, light-driven reduction of NADP + in the chloroplast is the main source of reducing equivalents in the form of NADPH...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2012) 445 (3): 313–322.
Published: 13 July 2012
... superfamily (MFS) transporter MDR1 (multidrug resistance 1) site-directed mutagenesis The overexpression of the drug efflux pumps encoding the genes CDR ( Candida drug resistance) 1 and CDR2 of the ABC (ATP-binding cassette) family of transporters and MDR1 (multidrug resistance 1...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2012) 444 (3): 437–443.
Published: 29 May 2012
... ′-diacetylchitobiosyl fluoride [α-(GlcNAc) 2 -F] site-directed mutagenesis Chitin oligosaccharides (GlcNAc) n [a β-1,4-linked polysaccharide of GlcNAc ( N -acetylglucosamine) with a polymerization degree of n ] have been drawing attention because of their presumed biological functions, such as inhibition...
Includes: Supplementary data
Articles
Benjamin Selles, Martin Hugo, Madia Trujillo, Vaibhav Srivastava, Gunnar Wingsle, Jean-Pierre Jacquot, Rafael Radi, Nicolas Rouhier
Journal:
Biochemical Journal
Biochem J (2012) 442 (2): 369–380.
Published: 13 February 2012
... 17 11 2011 29 11 2011 29 11 2011 © The Authors Journal compilation © 2012 Biochemical Society 2012 glutathione peroxidase over-oxidation peroxynitrite redox property site-directed mutagenesis thiol peroxidase In plants, various metabolic pathways or physiological...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2012) 442 (2): 357–368.
Published: 13 February 2012
... cerevisiae site-directed mutagenesis The capacity of a growing number of proteins to ‘moonlight’ – that is, to develop several functions within a single polypeptide chain that often are apparently unrelated – represents a way to expand the functional capabilities of an organism in spite...
Includes: Supplementary data
Articles
Anne Pribat, Rodnay Sormani, Mathieu Rousseau-Gueutin, Magdalena M. Julkowska, Christa Testerink, Jerôme Joubès, Michel Castroviejo, Michel Laguerre, Christian Meyer, Véronique Germain, Christophe Rothan
Journal:
Biochemical Journal
Biochem J (2012) 441 (1): 161–171.
Published: 14 December 2011
...-bisphosphate) and PI(3,5)P 2 (phosphatidylinositol 3,5-bisphosphate). In contrast with animal PTENs, PI(3,4,5)P 3 (phosphatidylinositol 3,4,5-trisphosphate) is a poor substrate. Site-directed mutagenesis of AtPTEN2a and molecular modelling of protein–phosphoinositide interactions indicated that substitutions...
Includes: Supplementary data
Articles
Efthalia Zervoudi, Athanasios Papakyriakou, Dimitra Georgiadou, Irini Evnouchidou, Anna Gajda, Marcin Poreba, Guy S. Salvesen, Marcin Drag, Akira Hattori, Luc Swevers, Dionisios Vourloumis, Efstratios Stratikos
Journal:
Biochemical Journal
Biochem J (2011) 435 (2): 411–420.
Published: 29 March 2011
... 2011 aminopeptidase antigen kinetics peptide specificity site-directed mutagenesis substrate library © The Authors Journal compilation © 2011 Biochemical Society 2011 Cytotoxic T-lymphocytes identify infected or transformed cells by recognizing small antigenic peptides bound...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2011) 434 (3): 459–471.
Published: 24 February 2011
... 2010 © The Authors Journal compilation © 2011 Biochemical Society 2011 aldehyde dehydrogenase (ALDH) coenzyme specificity enzymatic activity oxidative stress thiol regulation site-directed mutagenesis Given the high reactivity of ROS and the toxicity of the products generated...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2011) 433 (1): 65–74.
Published: 15 December 2010
... carboxylase (PEPC) phosphorylation site-specific antibodies protein phosphorylation Ricinus communis (castor oil plant) site-directed mutagenesis PEPC [PEP (phosphoenolpyruvate) carboxylase] (EC 4.1.1.31) is a tightly regulated cytosolic enzyme of vascular plants and green algae that catalyses...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2010) 432 (3): 445–454.
Published: 25 November 2010
... pyridoxal 5-phosphate synthase Saccharomyces cerevisiae site-directed mutagenesis The active form of vitamin B6, PLP (pyridoxal 5-phosphate), is an essential cofactor for numerous metabolic enzymes. PLP also has an important role in amino acid and carbohydrate metabolism [ 1 , 2 ]. To date, two...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2010) 432 (3): 565–573.
Published: 25 November 2010
... structure hydrogen bonding OpdA organophosphate-degrading enzyme organophosphate pesticide site-directed mutagenesis The intensive application of pesticides has facilitated the development and expansion of agriculture globally. Despite considerable evidence of environmental toxicity, the use...
Articles
Journal:
Biochemical Journal
Biochem J (2010) 432 (2): 237–247.
Published: 12 November 2010
... Journal compilation © 2010 Biochemical Society 2010 Bacillus subtilis inositol dehydrogenase myo inositol dehydrogenase site-directed mutagenesis structural classification X-ray crystallography myo -Inositol is ubiquitous in soil and plants. Inositol derivatives and isomers...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2010) 430 (3): 539–549.
Published: 27 August 2010
... ]. © The Authors Journal compilation © 2010 Biochemical Society 2010 autophosphorylation bacterium enzyme kinetics NTP synthesis nucleoside diphosphate kinase phosphotransfer activity site-directed mutagenesis NDK is well-known as a phosphoprotein and autophosphorylation...
Includes: Supplementary data
Articles
Ramon Hurtado-Guerrero, Tal Zusman, Shalini Pathak, Adel F. M. Ibrahim, Sharon Shepherd, Alan Prescott, Gil Segal, Daan M. F. van Aalten
Journal:
Biochemical Journal
Biochem J (2010) 426 (3): 281–292.
Published: 24 February 2010
... Society 2010 elongation factor 1A (EF1A) glucosyl transferase Legionella pneumophila microinjection site-directed mutagenesis protein structure 1 Correspondence may be addressed to either of these authors (email [email protected] or [email protected]...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2010) 426 (3): 337–344.
Published: 24 February 2010
... 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 archaeon argonaute double-stranded RNA (dsRNA) ribonuclease H RNA–DNA duplex site-directed mutagenesis RNase H (ribonuclease H) is a ubiquitous enzyme found in all three kingdoms of the tree of life: archaea...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2009) 419 (1): 75–86.
Published: 13 March 2009
...-binding properties, copper-IMAC experiments and site-directed mutagenesis based on computational modelling, were coupled with top-down MS [electrospray-ionization MS and MS/MS (tandem MS)]. Immobilized-copper-ion-affinity-chromatographic experiments allowed the primary characterization of the effects...
Includes: Supplementary data
Articles
Cédric Claperon, Raphael Rozenfeld, Xavier Iturrioz, Nicolas Inguimbert, Mayumi Okada, Bernard Roques, Bernard Maigret, Catherine Llorens-Cortes
Journal:
Biochemical Journal
Biochem J (2008) 416 (1): 37–46.
Published: 28 October 2008
... of this enzyme. Based on the X-ray crystal structure of LTA4H (leukotriene A4 hydrolase/aminopeptidase; EC 3.3.2.6) [ 11 ] and the functional data collected in our previous site-directed mutagenesis studies on APA [ 12 – 17 ], we built a three-dimensional model of the mouse APA ectodomain from residues 79...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2008) 416 (1): 109–116.
Published: 28 October 2008
... specificity toward synthetic substrates, its substrate specificity toward natural peptides is rather broad. Because of the pathophysiological significance of ERAP-1, it is important to elucidate the molecular basis of its enzymatic action. In the present study we used site-directed mutagenesis to identify...
Articles
Mark Frazzetto, Cenk Suphioglu, Jiuxiang Zhu, Oleg Schmidt-Kittler, Ian G. Jennings, Susan L. Cranmer, Shaun P. Jackson, Kenneth W. Kinzler, Bert Vogelstein, Philip E. Thompson
Journal:
Biochemical Journal
Biochem J (2008) 414 (3): 383–390.
Published: 27 August 2008
... isoforms of PI3K (phosphoinositide 3-kinase), yet little has been revealed about the molecular basis for the observed selectivities. Using site-directed mutagenesis, we have investigated one of the areas postulated as being critical to the observed selectivity. The residues Thr 886 and Lys 890 of the PI3Kγ...
Includes: Supplementary data
Articles
Carine Bebrone, Christine Anne, Frédéric Kerff, Gianpiero Garau, Kris De Vriendt, Raphaël Lantin, Bart Devreese, Jozef Van Beeumen, Otto Dideberg, Jean-Marie Frère, Moreno Galleni
Journal:
Biochemical Journal
Biochem J (2008) 414 (1): 151–159.
Published: 29 July 2008
... been performed by site-directed mutagenesis (H118A, D120A, D120T, H196A and H263A). To examine the effects of the charge or size of the residues on the substrate specificity, Val 67 was replaced by an alanine, isoleucine or aspartic acid residue, Thr 157 by an alanine residue, and Lys 224 and Lys...
Includes: Supplementary data
Articles
Paulo Durão, Zhenjia Chen, Catarina S. Silva, Cláudio M. Soares, Manuela M. Pereira, Smilja Todorovic, Peter Hildebrandt, Isabel Bento, Peter F. Lindley, Lígia O. Martins
Journal:
Biochemical Journal
Biochem J (2008) 412 (2): 339–346.
Published: 14 May 2008
.... 1 To whom correspondence should be addressed (email [email protected] ). 21 1 2008 25 2 2008 28 2 2008 28 2 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 CotA laccase laccase multi-copper oxidase T1 copper site-directed mutagenesis...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2008) 411 (3): 467–473.
Published: 14 April 2008
...Cheng-Chin Kuo; Kuan-Yu Lin; Yau-Jung Hsu; Shu-Yu Lin; Yu-Tsen Lin; Gu-Gang Chang; Wei-Yuan Chou The role of general acid–base catalysis in the enzymatic mechanism of NADP + -dependent malic enzyme was examined by detailed steady-state kinetic studies through site-directed mutagenesis of the Tyr 91...
Articles
René Assenberg, Michelle Webb, Edward Connolly, Katherine Stott, Matthew Watson, Josie Hobbs, Jean O. Thomas
Journal:
Biochemical Journal
Biochem J (2008) 411 (3): 553–561.
Published: 14 April 2008
... © 2008 Biochemical Society 2008 acetylation high mobility group (HMG) box NMR spectroscopy non-histone protein site-directed mutagenesis Protein acetylation plays a critical role in the control of gene transcription. This is effected through modulation of chromatin packaging...
Articles
Journal:
Biochemical Journal
Biochem J (2008) 411 (1): 181–190.
Published: 13 March 2008
... (glutaminyl-peptide cyclotransferase QC) pyroglutamate (pGlu) hydrogen-bond network proton transfer site-directed mutagenesis synchrotron X-ray crystallography QCs (glutaminyl cyclases; glutaminyl-peptide cyclotransferases, EC 2.3.2.5) catalyse the conversion of protein N-terminal glutamine...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2008) 410 (3): 543–553.
Published: 27 February 2008
... the enzyme from DEPC inactivation. Site-directed mutagenesis experiments in which the 15 histidine residues in the enzyme were replaced individually with alanine corroborated the chemical modification studies and established the importance of His-335 in the methylase activity. No gross structural differences...
Articles
Journal:
Biochemical Journal
Biochem J (2008) 409 (2): 417–428.
Published: 21 December 2007
... Society 2008 dimer–tetramer equilibrium Diocleinae lectin recombinant lectin site-directed mutagenesis synthetic gene X-ray crystal structure Lectins are a structurally heterogeneous group of proteins of non-immune origin possessing at least one non-catalytic domain that binds...
Articles
Journal:
Biochemical Journal
Biochem J (2007) 408 (2): 149–159.
Published: 14 November 2007
... roles of Gln 98 and Met 134 residues for HMP kinase activity and of Ser 444 for TMP synthase activity were experimentally confirmed by site-directed mutagenesis. 1 To whom correspondence should be addressed (email [email protected] ). The nucleotide sequence data reported...
Articles
Journal:
Biochemical Journal
Biochem J (2007) 407 (2): 189–198.
Published: 25 September 2007
... [email protected] ). 4 5 2007 14 6 2007 4 7 2007 4 7 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 Bacillus megaterium fructo-oligosaccharide levansucrase site-directed mutagenesis Global regulatory responses allow bacilli to adapt...
Articles
Christelle Bonod-Bidaud, Mickaël Beraud, Elisabeth Vaganay, Frédéric Delacoux, Bernard Font, David J. S. Hulmes, Florence Ruggiero
Journal:
Biochemical Journal
Biochem J (2007) 405 (2): 299–306.
Published: 27 June 2007
... extracellular matrix metalloproteinase procollagen proteolytic processing site-directed mutagenesis transient cell transfection Amino acid sequence analysis was performed by automated Edman degradation using an Applied Biosystems 473A protein sequencer. For N-terminal sequencing of the TSPN-1 released...
Articles
Maurício F. M. Machado, Vanessa Rioli, Fernanda M. Dalio, Leandro M. Castro, Maria A. Juliano, Ivarne L. Tersariol, Emer S. Ferro, Luiz Juliano, Vitor Oliveira
Journal:
Biochemical Journal
Biochem J (2007) 404 (2): 279–288.
Published: 14 May 2007
... to wild-type and mutant TOP. The final protein concentration in the assays was 2 μM. neurolysin substrate and inhibitor specificity site-directed mutagenesis thimet oligopeptidase TOP (thimet oligopeptidase; EC 3.4.24.15) and NEL (neurolysin; EC 3.4.24.16), which were initially detected...
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