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Keywords: site-directed mutagenesis
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Articles
Arline Fernández-Silva, Ana L. Juárez-Vázquez, Lilian González-Segura, Javier Andrés Juárez-Díaz, Rosario A. Muñoz-Clares
Journal:
Biochemical Journal
Biochem J (2023) 480 (4): 259–281.
Published: 23 February 2023
... 2 2 2023 © 2023 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2023 bacterial metabolism enzyme kinetics molecular docking protein crystallography site-directed mutagenesis structure–function relationships In agreement with the well...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2021) 478 (14): 2871–2887.
Published: 23 July 2021
... 5 2021 18 6 2021 29 6 2021 30 6 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 electron transfer Geobacter multiheme cytochrome c NMR spectroscopy site-directed mutagenesis Geobacter bacteria...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2021) 478 (10): 1921–1941.
Published: 24 May 2021
.... A multifunctional plant degradation enzyme was constructed with three functional roles of absorption, degradation, and mineralization using amino acid sequence recombination and site-directed mutagenesis to modify the template of plant degradation enzyme. Finally, using the Taguchi experimental design-assisted...
Articles
Michelle C. Miller, Irina V. Nesmelova, Vladimir A. Daragan, Hans Ippel, Malwina Michalak, Aurelio Dregni, Herbert Kaltner, Jürgen Kopitz, Hans-Joachim Gabius, Kevin H. Mayo
Journal:
Biochemical Journal
Biochem J (2020) 477 (17): 3147–3165.
Published: 04 September 2020
... molecular dynamics NMR relaxation NMR spectroscopy site-directed mutagenesis Screening for up- or down-regulation of expression of distinct genes upon changing a specific parameter is a robust approach to identify key aspects of the cascade leading to a triggered cellular response. When...
Includes: Supplementary data
Articles
Yannan Wang, Laurie A. Graham, Zhifu Han, Robert Eves, Audrey K. Gruneberg, Robert L. Campbell, Heqiao Zhang, Peter L. Davies
Journal:
Biochemical Journal
Biochem J (2020) 477 (12): 2179–2192.
Published: 22 June 2020
.... Unexpectedly, close examination of its surface did not reveal any large regions of flat, regularly spaced hydrophobic residues that characterize the ice-binding sites (IBSs) of potent antifreeze proteins from freeze-resistant fish and insects. An IBS was defined by site-directed mutagenesis of residues...
Includes: Multimedia, Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2020) 477 (11): 2095–2114.
Published: 15 June 2020
...Rosario A. Muñoz-Clares; Lilian González-Segura; Javier Andrés Juárez-Díaz; Carlos Mújica-Jiménez Activation of phosphoenolpyruvate carboxylase (PEPC) enzymes by glucose 6-phosphate (G6P) and other phospho-sugars is of major physiological relevance. Previous kinetic, site-directed mutagenesis...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2020) 477 (7): 1241–1259.
Published: 09 April 2020
... inhibition kinetics metalloproteases protein crystallography site-directed mutagenesis Angiotensin-converting enzyme (ACE, EC 3.4.15.1) is a zinc metalloprotease which has traditionally been known for its role in blood pressure regulation via the renin–angiotensin–aldosterone system (RAAS). During...
Articles
Journal:
Biochemical Journal
Biochem J (2019) 476 (24): 3835–3847.
Published: 23 December 2019
... Nampoothiri ( madhavan@niist.res.in ) 5 11 2019 6 12 2019 9 12 2019 9 12 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 C. glutamicum class E sortase peptide cleavage site-directed mutagenesis substrate...
Articles
Journal:
Biochemical Journal
Biochem J (2019) 476 (21): 3333–3353.
Published: 15 November 2019
... in the active site, helped us to propose a mechanism to further elucidate the catalytic cycle. c-di-GMP cGAMP crystallography phosphodiesterase second messenger site-directed mutagenesis The gene encoding a putative uncharacterized EAL domain ( Vc EAL) which contains amino acids 1–257...
Includes: Supplementary data
Articles
Yingxue Yang, Esther D. Lenherr, Roland Gromes, Shanshan Wang, Markus Wirtz, Rüdiger Hell, Tanja Peskan-Berghöfer, Klaus Scheffzek, Thomas Rausch
Journal:
Biochemical Journal
Biochem J (2019) 476 (7): 1191–1203.
Published: 15 April 2019
... access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY-NC-ND) . glutamylcysteine ligase glutathione biosynthesis homo-dimerization redox activation site-directed mutagenesis...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2018) 475 (23): 3887–3901.
Published: 12 December 2018
... of the Biochemical Society 2018 homoserine malate dehydrogenase protein engineering site-directed mutagenesis synthetic biology A central challenge in the creation of a resource-efficient and sustainable bioeconomy lies in the reduction in our dependence on fossil resources and a shift in focus...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2018) 475 (23): 3861–3873.
Published: 10 December 2018
... into a pUC57 vector such that expression was transcriptionally driven by the lacZ promoter [ 4 ]. Using these plasmids, mutations at specific conserved sites were generated with the QuikChange site-directed mutagenesis kit (Agilent Technologies, U.S.A.), following the manufacturer's instructions. Residues...
Includes: Supplementary data
Articles
Maike K. Groenewold, Marco Massmig, Stefanie Hebecker, Linna Danne, Zofia Magnowska, Manfred Nimtz, Franz Narberhaus, Dieter Jahn, Dirk W. Heinz, Lothar Jänsch, Jürgen Moser
Journal:
Biochemical Journal
Biochem J (2018) 475 (11): 1885–1907.
Published: 06 June 2018
... of phospholipid metabolism. Structure-guided site-directed mutagenesis was used to explore the proposed ligand-binding cavity of PA3911. Protein variants of Leu 56 , Leu 58 , Val 69 and Leu 114 were shown to impair PA interaction. A comparative shotgun lipidomics approach demonstrated a multifaceted response of P...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2017) 474 (11): 1807–1821.
Published: 16 May 2017
... Saccharomyces cerevisiae site-directed mutagenesis transmembrane proteins transport The electrochemical proton gradient acts as the driving force for the uptake and efflux of many metabolites and ions across the plasma membrane of fungi and plants [ 1 , 2 ]. A large number of transporters...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2016) 473 (21): 4011–4026.
Published: 27 October 2016
... 9 9 2016 © 2016 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2016 cold adaptation crystallography ice growth inhibition molecular docking site-directed mutagenesis thermal hysteresis Organisms that are exposed to subzero...
Articles
Mohammad Zulkifli, Shambhu Yadav, Anil Thakur, Shiffalli Singla, Monika Sharma, Anand Kumar Bachhawat
Journal:
Biochemical Journal
Biochem J (2016) 473 (15): 2369–2382.
Published: 28 July 2016
...-directed mutagenesis substrate specificity transmembrane domain Glutathione (γ-glutamyl- L -cysteinyl-glycine) is an essential metabolite in all unicellular and multicellular eukaryotes, barring a few amitochondrial organisms [ 1 , 2 ]. Glutathione defends against oxidative stress...
Includes: Supplementary data
Articles
Shoko Shinya, Shigenori Nishimura, Yoshihito Kitaoku, Tomoyuki Numata, Hisashi Kimoto, Hideo Kusaoke, Takayuki Ohnuma, Tamo Fukamizo
Journal:
Biochemical Journal
Biochem J (2016) 473 (8): 1085–1095.
Published: 08 April 2016
... CBM32 chitosan crystal structure NMR spectroscopy isothermal titration calorimetry site-directed mutagenesis Interactions between soil bacteria and fungal pathogens play important roles in sustainably managing agricultural production from soil [ 1 ]. Fungal cell walls contain several kinds...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2015) 471 (2): 187–198.
Published: 02 October 2015
... phosphatase activity on Cg Pmu2 by creating fusions of sections of CgPMU2 with CgPMU1 , a paralogue with little broad range phosphatase activity. We used site-directed mutagenesis on various fusions to sequentially convert Cg Pmu1 to Cg Pmu2. Based on molecular modelling of the Pmu proteins on to a histidine...
Articles
Dolores Linde, Rebecca Pogni, Marina Cañellas, Fátima Lucas, Victor Guallar, Maria Camilla Baratto, Adalgisa Sinicropi, Verónica Sáez-Jiménez, Cristina Coscolín, Antonio Romero, Francisco Javier Medrano, Francisco J. Ruiz-Dueñas, Angel T. Martínez
Journal:
Biochemical Journal
Biochem J (2015) 466 (2): 253–262.
Published: 20 February 2015
... spectroscopy molecular docking QM/MM site-directed mutagenesis Figure 1 Folding of A. auricula-judae DyP and location of exposed aromatic residues ( A ) General folding constituted by two domains, each of them including two large β-sheets and two or three helices, with the haem cofactor...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2014) 461 (1): 75–85.
Published: 13 June 2014
.... heterotrimeric G-protein plant G-protein site-directed mutagenesis GmGα protein soya bean G-protein activity yeast complementation 1 To whom correspondence should be addressed (email spandey@danforthcenter.org ). 8 10 2013 27 3 2014 2 4 2014 2 4 2014 ©...
Articles
Guillaume Née, Magali Aumont-Nicaise, Mirko Zaffagnini, Sylvie Nessler, Marielle Valerio-Lepiniec, Emmanuelle Issakidis-Bourguet
Journal:
Biochemical Journal
Biochem J (2014) 457 (1): 117–125.
Published: 10 December 2013
... binding to its active site. dithiol/disulfide interchange glucose-6-phosphate dehydrogenase redox regulation site-directed mutagenesis thioredoxin The 3D structure of the complete G6PDH1 protein (UniProtKB entry Q43727, 576 amino acids) was modelled using Swiss-Model software ( http...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2012) 445 (3): 313–322.
Published: 13 July 2012
... nitrogen base, 0.2% dropout mix and 2% glucose; Difco). For the agar plates, 2.5% (w/v) Bacto agar (Difco) was added to the medium. The site-directed mutagenesis was performed using the QuikChange® site-directed mutagenesis kit (Stratagene) as described previously [ 20 ]. The mutations were...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2012) 444 (3): 437–443.
Published: 29 May 2012
... available commercially. chitin oligosaccharide chitobiose phosphorylase glycosynthase α- N , N ′-diacetylchitobiosyl fluoride [α-(GlcNAc) 2 -F] site-directed mutagenesis Chitin oligosaccharides (GlcNAc) n [a β-1,4-linked polysaccharide of GlcNAc ( N -acetylglucosamine...
Includes: Supplementary data
Articles
Benjamin Selles, Martin Hugo, Madia Trujillo, Vaibhav Srivastava, Gunnar Wingsle, Jean-Pierre Jacquot, Rafael Radi, Nicolas Rouhier
Journal:
Biochemical Journal
Biochem J (2012) 442 (2): 369–380.
Published: 13 February 2012
.... The flow rate was fixed at 0.25 ml·min −1 and detection was recorded at 280 nm. The column was calibrated by loading 100 μl of bovine milk lactoserum [ 24 ]. glutathione peroxidase over-oxidation peroxynitrite redox property site-directed mutagenesis thiol peroxidase In plants, various...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2012) 442 (2): 357–368.
Published: 13 February 2012
... times in total. After sedimentation at 500 g for 10 min at 4°C, the cleared lysate was recovered and the protein concentration was quantified by a Bradford assay. CoA biosynthesis multifunctional protein protein phosphatase regulatory subunit Saccharomyces cerevisiae site-directed...
Includes: Supplementary data
Articles
Anne Pribat, Rodnay Sormani, Mathieu Rousseau-Gueutin, Magdalena M. Julkowska, Christa Testerink, Jerôme Joubès, Michel Castroviejo, Michel Laguerre, Christian Meyer, Véronique Germain, Christophe Rothan
Journal:
Biochemical Journal
Biochem J (2012) 441 (1): 161–171.
Published: 14 December 2011
...-bisphosphate) and PI(3,5)P 2 (phosphatidylinositol 3,5-bisphosphate). In contrast with animal PTENs, PI(3,4,5)P 3 (phosphatidylinositol 3,4,5-trisphosphate) is a poor substrate. Site-directed mutagenesis of AtPTEN2a and molecular modelling of protein–phosphoinositide interactions indicated that substitutions...
Includes: Supplementary data
Articles
Efthalia Zervoudi, Athanasios Papakyriakou, Dimitra Georgiadou, Irini Evnouchidou, Anna Gajda, Marcin Poreba, Guy S. Salvesen, Marcin Drag, Akira Hattori, Luc Swevers, Dionisios Vourloumis, Efstratios Stratikos
Journal:
Biochemical Journal
Biochem J (2011) 435 (2): 411–420.
Published: 29 March 2011
... from different plates. © The Authors Journal compilation © 2011 Biochemical Society 2011 aminopeptidase antigen kinetics peptide specificity site-directed mutagenesis substrate library Cytotoxic T-lymphocytes identify infected or transformed cells by recognizing small...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2011) 434 (3): 459–471.
Published: 24 February 2011
... 2010 © The Authors Journal compilation © 2011 Biochemical Society 2011 aldehyde dehydrogenase (ALDH) coenzyme specificity enzymatic activity oxidative stress thiol regulation site-directed mutagenesis The harmful effect of surplus amounts of aldehydes is well established [ 3...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2011) 433 (1): 65–74.
Published: 15 December 2010
.... oil seed metabolism phosphoenolpyruvate carboxylase (PEPC) phosphorylation site-specific antibodies protein phosphorylation Ricinus communis (castor oil plant) site-directed mutagenesis PEPC [PEP (phosphoenolpyruvate) carboxylase] (EC 4.1.1.31) is a tightly regulated cytosolic enzyme...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2010) 432 (3): 445–454.
Published: 25 November 2010
... pyridoxal 5-phosphate synthase Saccharomyces cerevisiae site-directed mutagenesis The active form of vitamin B6, PLP (pyridoxal 5-phosphate), is an essential cofactor for numerous metabolic enzymes. PLP also has an important role in amino acid and carbohydrate metabolism [ 1 , 2 ]. To date, two...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2010) 432 (3): 565–573.
Published: 25 November 2010
... 23 9 2010 24 9 2010 24 9 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 Agrobacterium radiobacter crystal structure hydrogen bonding OpdA organophosphate-degrading enzyme organophosphate pesticide site-directed mutagenesis The intensive...
Articles
Journal:
Biochemical Journal
Biochem J (2010) 432 (2): 237–247.
Published: 12 November 2010
... apoenzyme and inhibitor complex: probing the subunit interface with site-directed mutagenesis Proteins 2008 70 176 187 59 Williamson M. P. The structure and function of proline-rich regions in proteins Biochem. J. 1994 297 249 260 60 Stapley B. J. Creamer T. P...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2010) 430 (3): 539–549.
Published: 27 August 2010
... to discriminate between such cations [ 30 ]. © The Authors Journal compilation © 2010 Biochemical Society 2010 autophosphorylation bacterium enzyme kinetics NTP synthesis nucleoside diphosphate kinase phosphotransfer activity site-directed mutagenesis Figure 1 Autophosphorylation...
Includes: Supplementary data
Articles
Ramon Hurtado-Guerrero, Tal Zusman, Shalini Pathak, Adel F. M. Ibrahim, Sharon Shepherd, Alan Prescott, Gil Segal, Daan M. F. van Aalten
Journal:
Biochemical Journal
Biochem J (2010) 426 (3): 281–292.
Published: 24 February 2010
...://www.BiochemJ.org/bj/426/bj4260281add.htm ), and the PCR products were cloned directly into the bacterial expression vector pGEX6P1 (GE Healthcare). Site-directed mutagenesis was carried out following the QuikChange® site-directed mutagenesis protocol (Stratagene), using the KOD HotStart DNA polymerase...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2010) 426 (3): 337–344.
Published: 24 February 2010
... 2010 5 1 2010 5 1 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 archaeon argonaute double-stranded RNA (dsRNA) ribonuclease H RNA–DNA duplex site-directed mutagenesis A structural model was constructed using the molecular graphics software...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2009) 419 (1): 75–86.
Published: 13 March 2009
...-binding properties, copper-IMAC experiments and site-directed mutagenesis based on computational modelling, were coupled with top-down MS [electrospray-ionization MS and MS/MS (tandem MS)]. Immobilized-copper-ion-affinity-chromatographic experiments allowed the primary characterization of the effects...
Includes: Supplementary data
Articles
Cédric Claperon, Raphael Rozenfeld, Xavier Iturrioz, Nicolas Inguimbert, Mayumi Okada, Bernard Roques, Bernard Maigret, Catherine Llorens-Cortes
Journal:
Biochemical Journal
Biochem J (2008) 416 (1): 37–46.
Published: 28 October 2008
... design molecular modelling monozinc aminopeptidase site-directed mutagenesis APA (aminopeptidase A; EC 3.4.11.7) is a 160 kDa homodimeric type II membrane-bound aminopeptidase from the MA clan of the gluzincin family of metalloproteases. This enzyme preferentially cleaves the N-terminal...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2008) 416 (1): 109–116.
Published: 28 October 2008
... specificity toward synthetic substrates, its substrate specificity toward natural peptides is rather broad. Because of the pathophysiological significance of ERAP-1, it is important to elucidate the molecular basis of its enzymatic action. In the present study we used site-directed mutagenesis to identify...
Articles
Mark Frazzetto, Cenk Suphioglu, Jiuxiang Zhu, Oleg Schmidt-Kittler, Ian G. Jennings, Susan L. Cranmer, Shaun P. Jackson, Kenneth W. Kinzler, Bert Vogelstein, Philip E. Thompson
Journal:
Biochemical Journal
Biochem J (2008) 414 (3): 383–390.
Published: 27 August 2008
... isoforms of PI3K (phosphoinositide 3-kinase), yet little has been revealed about the molecular basis for the observed selectivities. Using site-directed mutagenesis, we have investigated one of the areas postulated as being critical to the observed selectivity. The residues Thr 886 and Lys 890 of the PI3Kγ...
Includes: Supplementary data
Articles
Carine Bebrone, Christine Anne, Frédéric Kerff, Gianpiero Garau, Kris De Vriendt, Raphaël Lantin, Bart Devreese, Jozef Van Beeumen, Otto Dideberg, Jean-Marie Frère, Moreno Galleni
Journal:
Biochemical Journal
Biochem J (2008) 414 (1): 151–159.
Published: 29 July 2008
... 2008 22 5 2008 23 5 2008 23 5 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 β-lactamase carbapenem enzymatic kinetics site-directed mutagenesis substrate binding zinc-binding site In vivo , class B β-lactamases [ 1 ] require one or two...
Includes: Supplementary data
Articles
Paulo Durão, Zhenjia Chen, Catarina S. Silva, Cláudio M. Soares, Manuela M. Pereira, Smilja Todorovic, Peter Hildebrandt, Isabel Bento, Peter F. Lindley, Lígia O. Martins
Journal:
Biochemical Journal
Biochem J (2008) 412 (2): 339–346.
Published: 14 May 2008
.... 21 1 2008 25 2 2008 28 2 2008 28 2 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 CotA laccase laccase multi-copper oxidase T1 copper site-directed mutagenesis 1 To whom correspondence should be addressed (email lmartins...
Includes: Supplementary data
Articles
René Assenberg, Michelle Webb, Edward Connolly, Katherine Stott, Matthew Watson, Josie Hobbs, Jean O. Thomas
Journal:
Biochemical Journal
Biochem J (2008) 411 (3): 553–561.
Published: 14 April 2008
... are shown. Note that the gels for the wild-type A domain and AB di-domain are reproduced from [ 23 ] with permission. © 2001 Elsevier. acetylation high mobility group (HMG) box NMR spectroscopy non-histone protein site-directed mutagenesis Protein acetylation plays a critical role...
Articles
Journal:
Biochemical Journal
Biochem J (2008) 411 (3): 467–473.
Published: 14 April 2008
...Cheng-Chin Kuo; Kuan-Yu Lin; Yau-Jung Hsu; Shu-Yu Lin; Yu-Tsen Lin; Gu-Gang Chang; Wei-Yuan Chou The role of general acid–base catalysis in the enzymatic mechanism of NADP + -dependent malic enzyme was examined by detailed steady-state kinetic studies through site-directed mutagenesis of the Tyr 91...
Articles
Journal:
Biochemical Journal
Biochem J (2008) 411 (1): 181–190.
Published: 13 March 2008
... pathways, such as the participation of Asp 248 and Asp 305 , in the catalysis process, could not be completely ruled out thus far. glutaminyl cyclase (glutaminyl-peptide cyclotransferase QC) pyroglutamate (pGlu) hydrogen-bond network proton transfer site-directed mutagenesis synchrotron X-ray...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2008) 410 (3): 543–553.
Published: 27 February 2008
... the enzyme from DEPC inactivation. Site-directed mutagenesis experiments in which the 15 histidine residues in the enzyme were replaced individually with alanine corroborated the chemical modification studies and established the importance of His-335 in the methylase activity. No gross structural differences...
Articles
Journal:
Biochemical Journal
Biochem J (2008) 409 (2): 417–428.
Published: 21 December 2007
... Society 2008 dimer–tetramer equilibrium Diocleinae lectin recombinant lectin site-directed mutagenesis synthetic gene X-ray crystal structure Lectins are a structurally heterogeneous group of proteins of non-immune origin possessing at least one non-catalytic domain that binds...
Articles
Journal:
Biochemical Journal
Biochem J (2007) 408 (2): 149–159.
Published: 14 November 2007
... roles of Gln 98 and Met 134 residues for HMP kinase activity and of Ser 444 for TMP synthase activity were experimentally confirmed by site-directed mutagenesis. 1 To whom correspondence should be addressed (email rapala@mol.uj.edu.pl ). The nucleotide sequence data reported...
Articles
Journal:
Biochemical Journal
Biochem J (2007) 407 (2): 189–198.
Published: 25 September 2007
... (email j.seibel@tu-bs.de ). 4 5 2007 14 6 2007 4 7 2007 4 7 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 Bacillus megaterium fructo-oligosaccharide levansucrase site-directed mutagenesis Global regulatory responses allow bacilli...
Articles
Christelle Bonod-Bidaud, Mickaël Beraud, Elisabeth Vaganay, Frédéric Delacoux, Bernard Font, David J. S. Hulmes, Florence Ruggiero
Journal:
Biochemical Journal
Biochem J (2007) 405 (2): 299–306.
Published: 27 June 2007
... extracellular matrix metalloproteinase procollagen proteolytic processing site-directed mutagenesis transient cell transfection Most extracellular matrix proteins are synthesized by cells as precursors that undergo subsequent proteolytic processing into mature and functional molecules. Among...
Articles
Maurício F. M. Machado, Vanessa Rioli, Fernanda M. Dalio, Leandro M. Castro, Maria A. Juliano, Ivarne L. Tersariol, Emer S. Ferro, Luiz Juliano, Vitor Oliveira
Journal:
Biochemical Journal
Biochem J (2007) 404 (2): 279–288.
Published: 14 May 2007
... to wild-type and mutant TOP. The final protein concentration in the assays was 2 μM. neurolysin substrate and inhibitor specificity site-directed mutagenesis thimet oligopeptidase TOP (thimet oligopeptidase; EC 3.4.24.15) and NEL (neurolysin; EC 3.4.24.16), which were initially detected...
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