...). Published by Portland Press Limited on behalf of the Biochemical Society 2016 biosynthetic diversity convergent evolution polyamine putrescine spermidine spermine Although it was previously described as the eukaryotic ODC, the AR-fold ODC is also found in diverse bacteria...

...Swati Mandal; Ajeet Mandal; Myung Hee Park The polyamines putrescine, spermidine and spermine are intimately involved in the regulation of cellular growth and viability. Transduction of human embryonic kidney (HEK) 293T cells with an adenovirus encoding a key polyamine catabolic enzyme, spermidine...

..., including cancer. The highly inducible enzymes SSAT (spermidine/spermine N 1 -acetyltransferase) and SMO (spermine oxidase) and the generally constitutively expressed APAO (N 1 -acetylpolyamine oxidase) appear to play critical roles in many normal and disease processes. The dysregulation of polyamine...

...Rafael Romero-Calderón; David E. Krantz Polyamine transport activities have been described in diverse multicellular systems, but their bioenergetic mechanisms and molecular identity remain unclear. In the present paper, we describe a high-affinity spermine/spermidine transport activity expressed...

...Oleg Nisenberg; Anthony E. Pegg; Patricia A. Welsh; Kerry Keefer; Lisa M. Shantz The present study was designed to provide a better understanding of the role played by AdoMetDC ( S -adenosylmethionine decarboxylase), the key rate-controlling enzyme in the synthesis of spermidine and spermine...

... and the tissue studied. Very large increases in SpmS activity were seen in many tissues. SpdS (spermidine synthase) activity was not affected. Although there was a statistically significant decline in spermidine content and increase in spermine, the alterations were small compared with the increase in SpmS...

... spermidine spermine Abbreviations used: AZ, antizyme; AZI, AZ inhibitor; cdk, cyclin-dependent kinase; CHENSpm, N 1 -ethyl- N 4 -[(cycloheptyl)methyl]-4,8-diazaundecane; dcSAM, decarboxylated S -adenosylmethionine; DFMO, α-difluoromethylornithine; MDL 72527, N 1 , N 4 -bis(buta-2,3-dienyl...

... of ornithine decarboxylase (the first enzyme of the polyamine biosynthetic pathway), induction of polyamine oxidase and spermidine/spermine N 1 -acetyltransferase (the enzymes responsible for polyamine catabolism) and induction of polyamine export all contributed to the decreased intracellular polyamine...

...Ying CHEN; Slavoljub VUJCIC; Ping LIANG; Paula DIEGELMAN; Debora L. KRAMER; Carl W. PORTER In the polyamine back-conversion pathway, spermine and spermidine are first acetylated by spermidine/spermine N 1 -acetyl-transferase (SSAT-1) and then oxidized by polyamine oxidase to produce spermidine...

...Slavoljub VUJCIC; Ping LIANG; Paula DIEGELMAN; Debora L. KRAMER; Carl W. PORTER In the polyamine back-conversion pathway, spermine and spermidine are first acetylated by spermidine/spermine N 1 -acetyltransferase (SSAT) and then oxidized by polyamine oxidase (PAO) to produce spermidine...

... numbers AY033890, AY033891 and AF519179 respectively. 9 10 2002 23 10 2002 24 10 2002 24 10 2002 The Biochemical Society, London ©2002 2002 N 1 -acetylspermine isoform polyamines spermidine spermine Abbreviations used: N 1 -AcSpm, N 1 -acetylspermine...

...Slavoljub VUJCIC; Paula DIEGELMAN; Cyrus J. BACCHI; Debora L. KRAMER; Carl W. PORTER During polyamine catabolism, spermine and spermidine are first acetylated by spermidine/spermine N 1 -acetyltransferase (SSAT) and subsequently oxidized by polyamine oxidase (PAO) to produce spermidine...

...John L.A. MITCHELL; Aviva LEYSER; Michelle S. HOLTORFF; Jill S. BATES; Benjamin FRYDMAN; Aldonia L. VALASINAS; Venodhar K. REDDY; Laurence J. MARTON The polyamines spermidine and spermine and their diamine precursor putrescine are essential for mammalian cell growth and viability, and strategies...

...Sandra L. OZA; Mark R. ARIYANAYAGAM; Alan H. FAIRLAMB Trypanothione [ N 1 , N 8 -bis(glutathionyl)spermidine] is a unique metabolite found only in trypanosomatids, where it subsumes many of the functions of GSH in other organisms. In Crithidia fasciculata , two distinct ATP-dependent ligases...

... initiation factor 5A (eIF5A) at the third day of culture. There was also a marked decrease in cellular putrescine content and a small decrease in spermidine content. Accumulation of decapped mRNA, which is typically associated with eIF5A deficiency in yeast, was also observed. The inhibition of cell growth...

... by bafilomycin A 1 , an inhibitor of the vacuolar H + -ATPase. Among the four polyamine transporters, those encoded by TPO2 and TPO3 were specific for spermine, whereas those encoded by TPO1 and TPO4 recognized spermidine and spermine. These results suggest that polyamine content in the cytoplasm of yeast...

...Chang Hoon LEE; Myung Hee PARK Deoxyhypusine synthase catalyses the NAD-dependent transfer of the butylamine moiety from the polyamine, spermidine, to a specific lysine residue of a single cellular protein, eukaryotic translation-initiation factor 5A (eIF5A) precursor. The native enzyme exists...

... showed that Gy males lacked any significant spermine synthase activity as well as spermine content. Organs of Gy males had a higher spermidine content. This was caused not only by the lack of conversion of spermidine into spermine, but also because of compensatory increases in the activities of other...

... the high levels of antizyme, endogenous ODC activity was not completely abolished, with 10– 39% remaining, depending on the transgenic line. Additionally, a relatively small decrease (30–32%) in cardiac spermidine content was observed, with levels of putrescine and spermine unaffected. Interestingly...

...Kazuei IGARASHI; Keiko KASHIWAGI The polyamine content of cells is regulated by biosynthesis, degradation and transport. In Escherichia coli , the genes for three different polyamine transport systems have been cloned and characterized. Two uptake systems (putrescine-specific and spermidine...