... [email protected] . 3 3 2016 20 4 2016 22 4 2016 © 2016 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2016 biosynthetic diversity convergent evolution polyamine putrescine spermidine spermine Polyamines are small...

...Swati Mandal; Ajeet Mandal; Myung Hee Park The polyamines putrescine, spermidine and spermine are intimately involved in the regulation of cellular growth and viability. Transduction of human embryonic kidney (HEK) 293T cells with an adenovirus encoding a key polyamine catabolic enzyme, spermidine...

... that in mice lacking spermine synthase there was a large increase in dcAdoMet and that overexpression of spermine synthase reduced the amount of this nucleoside. There was also an increase in dcAdoMet in cells derived from patients with Snyder–Robinson syndrome, a rare X-linked recessive human disease caused...

..., including cancer. The highly inducible enzymes SSAT (spermidine/spermine N 1 -acetyltransferase) and SMO (spermine oxidase) and the generally constitutively expressed APAO (N 1 -acetylpolyamine oxidase) appear to play critical roles in many normal and disease processes. The dysregulation of polyamine...

... of polyamine analogues has emerged as a viable strategy for therapeutic intervention. Previously, we showed that the naturally occurring polyamines spermidine and spermine were quite effective at inducing the oligomerization of nucleosomal arrays in vitro , suggesting that polyamines may play a key role...

...Rafael Romero-Calderón; David E. Krantz Polyamine transport activities have been described in diverse multicellular systems, but their bioenergetic mechanisms and molecular identity remain unclear. In the present paper, we describe a high-affinity spermine/spermidine transport activity expressed...

...Oleg Nisenberg; Anthony E. Pegg; Patricia A. Welsh; Kerry Keefer; Lisa M. Shantz The present study was designed to provide a better understanding of the role played by AdoMetDC ( S -adenosylmethionine decarboxylase), the key rate-controlling enzyme in the synthesis of spermidine and spermine...

.... The enzyme was purified 48500-fold by sequential chromatographies on hydroxyapatite, Con A (concanavalin A)–Sepharose, porcine colonic mucin–Sepharose, Cu 2+ -chelating Sepharose and AMP–agarose. Interestingly, the purified pGal3ST required submillimolar concentrations of spermine or basic lipids, such as D...

...Yoshihiko IKEGUCHI; Xiaojing WANG; Diane E. McCLOSKEY; Catherine S. COLEMAN; Paul NELSON; Guirong HU; Lisa M. SHANTZ; Anthony E. PEGG A widespread increase in SpmS (spermine synthase) activity has been produced in transgenic mice using a construct in which the human SpmS cDNA was placed under...

... spermidine spermine Abbreviations used: AZ, antizyme; AZI, AZ inhibitor; cdk, cyclin-dependent kinase; CHENSpm, N 1 -ethyl- N 4 -[(cycloheptyl)methyl]-4,8-diazaundecane; dcSAM, decarboxylated S -adenosylmethionine; DFMO, α-difluoromethylornithine; MDL 72527, N 1 , N 4 -bis(buta-2,3-dienyl...

...Jérôme BREDIN; Valérie SIMONET; Ramkumar IYER; Anne H. DELCOUR; Jean-Marie PAGÈS The L3 loop is an important feature of the OmpF porin structure, contributing to both channel size and electrostatic properties. Colicins A and N, spermine, and antibiotics that use OmpF to penetrate the cell, were...

... of ornithine decarboxylase (the first enzyme of the polyamine biosynthetic pathway), induction of polyamine oxidase and spermidine/spermine N 1 -acetyltransferase (the enzymes responsible for polyamine catabolism) and induction of polyamine export all contributed to the decreased intracellular polyamine...

...Yoshihiko IKEGUCHI; Caroline A. MACKINTOSH; Diane E. McCLOSKEY; Anthony E. PEGG The role of spermine in the sensitivity of cells to various established and experimental anti-tumour agents was examined, using paired cell lines that possess or lack spermine synthase. All spermine-synthase-deficient...

...Slavoljub VUJCIC; Ping LIANG; Paula DIEGELMAN; Debora L. KRAMER; Carl W. PORTER In the polyamine back-conversion pathway, spermine and spermidine are first acetylated by spermidine/spermine N 1 -acetyltransferase (SSAT) and then oxidized by polyamine oxidase (PAO) to produce spermidine...

... numbers AY033890, AY033891 and AF519179 respectively. 9 10 2002 23 10 2002 24 10 2002 24 10 2002 The Biochemical Society, London ©2002 2002 N 1 -acetylspermine isoform polyamines spermidine spermine Abbreviations used: N 1 -AcSpm, N 1 -acetylspermine...

...Alison V. FRASER; Patrick M. WOSTER; Heather M. WALLACE Human promyelogenous leukaemic cells (HL-60) were treated with novel spermine analogue, ( S )- N 1 -(2-methyl-1-butyl)- N 11 -ethyl-4,8-diazaundecane (IPENSpm), and the effects on growth and intracellular polyamine metabolism were measured...

...John L.A. MITCHELL; Aviva LEYSER; Michelle S. HOLTORFF; Jill S. BATES; Benjamin FRYDMAN; Aldonia L. VALASINAS; Venodhar K. REDDY; Laurence J. MARTON The polyamines spermidine and spermine and their diamine precursor putrescine are essential for mammalian cell growth and viability, and strategies...

... by bafilomycin A 1 , an inhibitor of the vacuolar H + -ATPase. Among the four polyamine transporters, those encoded by TPO2 and TPO3 were specific for spermine, whereas those encoded by TPO1 and TPO4 recognized spermidine and spermine. These results suggest that polyamine content in the cytoplasm of yeast...

... the high levels of antizyme, endogenous ODC activity was not completely abolished, with 10– 39% remaining, depending on the transgenic line. Additionally, a relatively small decrease (30–32%) in cardiac spermidine content was observed, with levels of putrescine and spermine unaffected. Interestingly...

... of the caspase cascade. In this report, we show that addition of micromolar concentrations of polyamines to isolated rat heart mitochondria induces the release of cytochrome c . Spermine, which is effective at concentrations of 10-100 μ M, is more potent than spermidine, whereas putrescine has no effect up to 1...

... and glutamic acid residues, which interact with the NH # (or NH ) groups, and by tryptophan and tyrosine residues that have hydrophobic INTRODUCTION Polyamines (putrescine, spermidine and spermine) are necessary for cell growth [1 3]. They are among the major polycations in cells, together with Ca#+ and Mg...

...Mattias BELTING; Susanne PERSSON; Lars-Åke FRANSSON We have evaluated the possible role of proteoglycans in the uptake of spermine by human lung fibroblasts. Exogenous glycosaminoglycans behaved as competitive inhibitors of spermine uptake, the most efficient being heparan sulphate ( K i = 0.16...