...; Jeffrey A. Scholten; Jay Wendling; Hideji Fujiwara; Olga Nemirovskiy; Arthur J. Wittwer; Marek M. Nagiec SphK (sphingosine kinase) is the major source of the bioactive lipid and GPCR (G-protein-coupled receptor) agonist S1P (sphingosine 1-phosphate). S1P promotes cell growth, survival and migration...

... the formation of ceramides from sphingoid base and acyl-CoA substrates. From a metabolic perspective, these enzymes occupy a unique niche in that they simultaneously regulate de novo sphingolipid synthesis and the recycling of free sphingosine produced from the degradation of pre-formed sphingolipids (salvage...

... an exogenous LCB (long-chain base) like PHS (phytosphingosine) or DHS (dihydrosphingosine). In the present study, supplying SPH (sphingosine), an unnatural LCB for yeast, similarly rescued the Δ lcb2 cells, but only when SPH 1-phosphate production was inhibited by deleting the LCB kinase gene LCB4...

... The Biochemical Society, London 2006 catalytically important serine residue neutral ceramidase novel amidase motif sphingosine dihydroCDase (YDC1p) phytoCDase (YPC1p) The rat brain neutral CDase has an apparent molecular mass of 90–95 kDa under reducing conditions and a pI (isoelectric point...

...Iyad Sultan; Can E. Senkal; Suriyan Ponnusamy; Jacek Bielawski; Zdzislaw Szulc; Alicja Bielawska; Yusuf A. Hannun; Besim Ogretmen In the present study, the regulation of the sphingosine-recycling pathway in A549 human lung adenocarcinoma cells by oxidative stress was investigated. The generation...

... -sphingosine and N , N -dimethylsphingosine, for enzymatic activity. pGal3ST recognized Galβ1→3GalNAc (core 1) as an optimal substrate, and had weaker activity for Galβ1→3GlcNAc (type 1) and Galβ1→4GlcNAc (type 2). Substrate competition experiments proved that a single enzyme catalyses sulphation of all three...

...Farida SAFADI-CHAMBERLAIN; Li-Ping WANG; Shawn G. PAYNE; Chang-Uk LIM; Suzanne STRATFORD; Jose Antonio CHAVEZ; Michael H. FOX; Sarah SPIEGEL; Scott A. SUMMERS Numerous extracellular stimuli activate SK1 (sphingosine kinase type 1) to catalyse the production of sphingosine 1-phosphate, a bioactive...

...Elisabetta MEACCI; Francesca CENCETTI; Chiara DONATI; Francesca NUTI; Laura BECCIOLINI; Paola BRUNI Sphingosine (Sph) has been implicated as a modulator of membrane signal transduction systems and as a regulatory element of cardiac and skeletal muscle physiology, but little information is presently...

... 2003 19 2 2004 4 3 2004 4 3 2004 The Biochemical Society, London ©2004 2004 ceramide mass isotopomer octadecane-1,2-diol sphingomyelin sphingosine stable isotope Abbreviations used: 16-cer, N -palmitoylsphingosine; 17-cer, N -heptadecanoylsphingosine; diH-16...