... does not aggregate into amyloid fibrils in vivo or in vitro . We examined the hydrophobic residues responsible for the high-stability and low-aggregation properties of murine transthyretin using site-directed mutagenesis. Urea-induced unfolding and thioflavin T fluorescence aggregation assay revealed...

... been described, but interpretation is problematic because discrimination is lacking between the effect of global changes in native stability and specific effects on structural mechanism. We show that the temperature midpoint ( T m ) of thermal denaturation reflects the transition of α 1 -antitrypsin...

... bond was not involved in determining the affinity of the enzyme towards its substrate, but had an important role in determining the stability of the protein, and its catalytic efficiency. We show that in vivo the γ-GCS enzyme can also exist in a reduced form and that the mutants lacking the disulfide...

... 2 3 2012 2 3 2012 angiomotin (AMOT) degradation neural-precursorcell-expressed developmentally down-regulated 4 (Nedd4) stability ubiquitination Yes-associated protein (YAP) Protein ubiquitination has emerged as a fundamental mechanism for regulating the half lives...

... in inactivation in various human cancers. RASSF1A is implicated in the regulation of apoptosis, microtubule stability and cell cycle arrest. However, little is known about the regulation and function of RASSF1C. In the present study we show that exogenously expressed RASSF1C is a very unstable protein...

...-mediated ubiquitination and degradation. Therefore the present study reveals that the C-terminus of PTEN plays a critical role in stabilizing PTEN via antagonizing NEDD4-1-induced PTEN protein decay; conversely, truncation of the PTEN C-terminus results in rapid NEDD4-1-mediated PTEN degradation...

... have analysed rat liver mtDNA and mtRNA (mitochondrial RNA) synthesis as well as their stability under the influence of in vivo treatment or in vitro exposure to cisplatin. We show that cisplatin causes a direct and significant impairment of mtDNA and mtRNA synthesis and decreases steady-state levels...

... autotransporter Ag43α, which induces frizzy colony morphology and cell settling. We identify 16 glycosylation sites and suggest two possible glycosylation motifs for serine and threonine residues. Glycosylation stabilizes against thermal and chemical denaturation and increases refolding kinetics. Unexpectedly...

...Paul Evans; Christine Slingsby; B. A. Wallace Age-related cataract is a result of crystallins, the predominant lens proteins, forming light-scattering aggregates. In the low protein turnover environment of the eye lens, the crystallins are susceptible to modifications that can reduce stability...

... the temperature of an enzyme assay. Determination of T eq to date has used continuous assays, because this method produces progress curves directly and obviates the need to perform separate activity and stability experiments, and has utilized enzymes whose reactions are essentially irreversible (far from...

[email protected] ). 20 4 2006 4 7 2006 7 7 2006 7 7 2006 The Biochemical Society, London 2006 HIV-1 immunogen design neutralizing antibody stability thioredoxin V3 loop It is well known that a significant fraction of strain-specific virus-neutralizing antibodies...

...Brett FEENEY; Cristina POP; Ashutosh TRIPATHY; A. Clay CLARK We have examined the role of a salt bridge between Lys 242 and Glu 246 in loop L4 of procaspase 3 and of mature caspase 3, and we show that the interactions are required for stabilizing the active site. Replacing either of the residues...

... previously been characterized structurally. Its tertiary structure is similar to that of DHFR from E. coli but it is the only DHFR characterized so far that relies on dimerization for stability. The midpoint of the thermal unfolding of TmDHFR was at approx. 83 °C, which was 30 °C higher than the melting...

... of the receptors. We have demonstrated that the NR1 subunit is efficiently transported to the plasma membrane in cells expressing NR1 alone, similarly to cells producing NR1 and NR2A together. In contrast, NR2A requires NR1 expression to be located at the cell surface. The stability of both receptor subunits...

... solution conformation. Key words: conformation, ESI-MS, H}D exchange, stability. conformational changes can be monitored with ESI-MS by observing changes in the charge-state distribution. In addition, hydrogen}deuterium exchange (H}D exchange) has made many advances in MS [13 15]. H}D exchange of proteins...

... localization of LPC in the trans- Golgi network. Finally, LPC could not be detected in detergent-resistant membrane rafts. Taken together, these results suggest that dynamic palmitoylation of LPC is important for stability, but does not function as a dominant trafficking signal. 1 Present address...

... in the presence of Fe(II) and Zn(II) ions with properties identical to the native protein. Nevertheless the protein has lower stability than native Da FdIII during chromatography. The one-dimensional 600 MHz NMR spectrum of the apoprotein indicates an unstructured protein with random coil chemical shifts whereas...