.... 2 11 2021 7 1 2022 28 1 2022 © 2022 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2022 AraC protein E coli genetic selection system random mutagenesis analysis retro viral protease substrate recognition The Human...

...). Published by Portland Press Limited on behalf of the Biochemical Society 2021 base excision repair DNA glycosylase substrate recognition Guanine:thymine mispairs are the most abundant mismatches found in genomic DNA ( Figure 1 ) [ 1–3 ]. These mismatches are produced by various pathways...

..., phosphatidic acid and 1-inositol phosphate. Comparisons of these structures with the wild-type SaPLD show a larger binding site able to accommodate a bulkier secondary alcohol substrate as well as changes to the position of a flexible surface loop proposed to be involved in substrate recognition. The complex...

... small-molecule inhibitors substrate recognition Fragile X syndrome (FXS) is a genetic disorder caused by a mutation in the Fragile X mental retardation 1 ( FMR1 ) gene [ 1 ]. Affected individuals show developmental delay and reduced intellectual abilities, often paired with autism, social...

...). Published by Portland Press Limited on behalf of the Biochemical Society 2019 catalytic mechanism crystallography histone desuccinylation Sirt5 substrate recognition Sirtuin 5 (Sirt5) belongs to the sirtuin (yeast Sir2-like proteins) protein family, which includes nicotinamide adenine...

... stabilization and molecular mechanism for substrate recognition of αTAT1 The cDNA encoding human αTAT1 of 333 amino acid residues [ 7 , 8 ] was cloned by PCR using Human Multiple Tissue cDNA panels (BD Biosciences) as a template. The cDNA fragment encoding αTAT1-N (amino acid residues 1–194) and αTAT1...

... to mammals. Structural and solution studies of range of related dicarboxylic acids suggest the decarboxylase activity is restricted to oxaloacetate as a substrate. decarboxylase mechanism enzyme evolution oxaloacetate analogue oxaloacetate decarboxylase pyruvate kinase substrate recognition...

... of β1,4-GalT7 fused with the maltose-binding protein to study substrate recognition. Calorimetric binding studies showed that the binding of the donor substrate UDP-Gal largely promoted binding of the acceptor substrate. To identify the structural basis governing substrate recognition, we used a fragment...

... in the function of MDR1. 1 To whom correspondence should be addressed (email [email protected] ). 27 4 2006 3 10 2006 10 10 2006 10 10 2006 The Biochemical Society, London 2007 cholesterol drug-binding pocket multidrug resistance substrate recognition...

.... These results indicate that TMs 1, 5, 7 and 8 are necessary for exhibiting high-affinity glucose-transport activity of Hxt2. chimaera glucose transporter saturation mutagenesis substrate recognition yeast Abbreviations used: MFS, major facilitator superfamily; TM, transmembrane segment. 1...

...%. These structures provide important new insights into substrate recognition and ligand-induced conformational changes. Comparison of these structures with the structures of OTCase complexed with the bisubstrate analogue PALO or CP and L -norvaline reveals that binding of the first substrate, CP, induces a global...

...Julio J. CARAMELO; Jorge FLORÍN-CHRISTENSEN; Mónica FLORÍN-CHRISTENSEN; José M. DELFINO A set of radioiodinatable phosphatidylcholines (PCs) derivatized with the Bolton-Hunter reagent (BHPCs) was synthesized to probe the substrate recognition and activity of phospholipases. A common feature...