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Keywords: substrate specificity
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Biochem J (2021) 478 (13): 2697–2713.
Published: 16 July 2021
... and M17 aminopeptidases are both postulated to have an essential role in the terminal stages of the hemoglobin digestion process and are validated drug targets for the design of new dual-target anti-malarial compounds. In this study, we profiled the substrate specificity fingerprints and kinetic behaviors...
Includes: Supplementary data
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Biochem J (2021) 478 (6): 1241–1259.
Published: 19 March 2021
... The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 binding assays GRIM-19 HtrA2 in silico modelling structural changes substrate specificity Uncontrolled cell proliferation due to the non-synchronous activity of pro and anti-apoptotic proteins...
Includes: Supplementary data
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Biochem J (2019) 476 (24): 3835–3847.
Published: 23 December 2019
... a high substrate specificity towards LAXTG recognition motif of class E sortase. However, in the literature reports there is no experimental records that support the sortase activity and substrate specificity of sortase E of C. glutamicum . Class E sortase are widely distributed in GC rich...
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Biochem J (2017) 474 (14): 2417–2433.
Published: 06 July 2017
... a combination of degradomic and substrate specificity assays, we identified candidate KLK9 substrates in two different epithelial cell lines [the non-tumorigenic human keratinocyte cells (HaCaT) and the tumorigenic tongue squamous carcinoma cells (SCC9)]. Two potential KLK9 substrates [KLK10 and midkine (MDK...
Includes: Supplementary data
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Biochem J (2016) 473 (19): 3189–3204.
Published: 27 September 2016
... strategy. Correspondence: Nyssa Drinkwater ( nyssa.drinkwater@monash.edu ) or Sheena McGowan ( sheena.mcgowan@monash.edu ) aminopeptidase crystallography malaria Plasmodium falciparum protease inhibitor substrate specificity In October 2015, the Nobel Prize for medicine...
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Biochem J (2016) 473 (7): 839–849.
Published: 29 March 2016
... heats of dilution were subtracted from binding isotherms. The resulting ITC data were fitted using Origin software to a one-site binding model. carbohydrate esterase crystallography ferulic acid esterase Neocallimastigomycota structure–function substrate specificity...
Includes: Multimedia, Supplementary data
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Biochem J (2012) 444 (2): e3–e5.
Published: 11 May 2012
... mechanism and the substrate specificity of MALT1. These intriguing findings convincingly demonstrate the importance of MALT1 dimerization for its catalytic activity and pave the way for novel therapeutic approaches that target this crucial regulator of lymphoma survival and proliferation. 1 To whom...
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Biochem J (2012) 443 (1): 287–295.
Published: 14 March 2012
... substrate specificity. 1 To whom correspondence should be addressed (email gsalvesen@sanfordburnham.org ). 4 1 2012 3 2 2012 6 2 2012 6 2 2012 © 2012 The Author(s) 2012 This is an open-access article distributed under the terms of the Creative Commons Attribution Non...
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Biochem J (2011) 435 (2): 345–354.
Published: 29 March 2011
...Tana Koudelakova; Eva Chovancova; Jan Brezovsky; Marta Monincova; Andrea Fortova; Jiri Jarkovsky; Jiri Damborsky An enzyme's substrate specificity is one of its most important characteristics. The quantitative comparison of broad-specificity enzymes requires the selection of a homogenous set...
Includes: Supplementary data
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Biochem J (2010) 431 (3): 401–412.
Published: 11 October 2010
... lysine biosynthesis substrate specificity Alteration of enzyme substrate specificity is important not only to create an enzyme applicable for practical use, but also to obtain better understanding of the mechanisms of both substrate recognition and catalytic function. The rational design...
Includes: Supplementary data
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Biochem J (2010) 431 (3): 337–347.
Published: 11 October 2010
... rjpeters@iastate.edu ). 19 4 2010 6 8 2010 11 8 2010 11 8 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 cytochrome P450 gibberellin hormone metabolism mono-oxygenase catalysis substrate specificity The importance of gibberellins...
Includes: Supplementary data
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Biochem J (2010) 430 (2): 335–344.
Published: 13 August 2010
...) substrate specificity SUMO-specific processing protease (SENP) SUMO (small ubiquitin-like protein modifier) family proteins share a conserved ubiquitin-like domain and a C-terminal di-glycine motif. The covalent modification of proteins by SUMO plays a dynamic role in transcriptional regulation...
Includes: Supplementary data
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Biochem J (2010) 428 (1): 25–32.
Published: 28 April 2010
...) structural motif structure–function relationship substrate specificity GST (glutathione transferase; EC 2.5.1.18) is eminently recognized as a detoxifying enzyme that is widely distributed in all organisms [ 1 ]. The enzymatic biotransformation within the active site is generally initiated...
Includes: Supplementary data
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Biochem J (2009) 421 (2): 231–241.
Published: 26 June 2009
... accepts many prenyl substrates of different chain lengths, LePGT1 can utilize only geranyl diphosphate as its prenyl substrate. Thus the substrate specificity was analysed using chimeric enzymes derived from LePGT1 and UBIA. In vitro and in vivo analyses of the chimeras suggested that the determinant...
Includes: Supplementary data
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Biochem J (2009) 418 (3): 605–614.
Published: 25 February 2009
..., partially governing the strict donor substrate specificity of β1,4-GalT1 [ 39 ]. Contrary to β1,4-GalT1, β1,4-GalT7 has the ability to bind other UDP-sugars than UDP-Gal, confirming the low contribution of the Gal moiety in binding and suggesting that the binding donor site has enough space to accommodate...
Includes: Supplementary data
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Biochem J (2008) 411 (1): 27–32.
Published: 13 March 2008
... differentiate their physiological roles, we compared the substrate specificities of Plk1 and Plk3 using synthetic peptides. Among these substrate peptides, topoisomerase IIα EKT 1342 DDE-containing synthetic peptide was strongly phosphorylated by Plk3 but not by Plk1. By modulating the topoisomerase IIα peptide...
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Biochem J (2007) 408 (3): 363–373.
Published: 28 November 2007
... be addressed (email claude.cochet@cea.fr ). 21 6 2007 22 8 2007 23 8 2007 23 8 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 CK2 protein kinase cyclic peptide hotspot peptide disruptor protein–protein interaction substrate specificity All...
Includes: Supplementary data
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Biochem J (2007) 405 (3): 429–438.
Published: 13 July 2007
... profiled the primary P4–P1 substrate specificity of FVIIa using positional scanning substrate combinatorial libraries and evaluated the role of the selective active site in defining specificity. Being a trypsin-like serine protease, FVIIa had P1 specificity exclusively towards arginine and lysine residues...
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Biochem J (2007) 403 (3): 421–430.
Published: 12 April 2007
... extent. Water-mediated interactions permit binding of substrates in multiple conformations in the spacious hydrophilic binding site, and correlate with the observed broad substrate specificity. 1 These authors contributed equally to this work. 2 To whom correspondence should be addressed...
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Biochem J (2006) 398 (1): 37–43.
Published: 27 July 2006
... of the anomeric configuration. The substrate specificity and reaction mechanism of cellobiose phosphorylase from Cellvibrio gilvus have been investigated in detail. We have determined the crystal structure of the glucose-sulphate and glucose-phosphate complexes of this enzyme at a maximal resolution of 2.0 Å (1...
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Biochem J (2005) 388 (1): 255–261.
Published: 10 May 2005
... folding substrate specificity thimet oligopeptidase urea Thimet oligopeptidase (TOP) (EC 3.4.24.15; EP24.15) is a 78-kDa Zn(II)-dependent endopeptidase [ 1 ]. It is related to several metallopeptidases, including angiotension-converting enzyme, neprilysin and neurolysin, all of which share...
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Biochem J (2005) 387 (2): 489–496.
Published: 05 April 2005
... substrate specificity Genes coding for most of the phospholipid-synthesizing enzymes have been cloned, and the properties as well as the regulation of the respective enzymes have been studied in great detail [ 1 , 2 ]. In contrast, much less is known about regulatory aspects of phospholipid...
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Biochem J (2005) 386 (1): 169–176.
Published: 08 February 2005
...) but similar k cat value (1.7±0.1 s −1 ) compared with FPP. Replacement of L85, L88 or F89 with Ala increases FPP and GGPP K m values by the same amount, indicating that these amino acids are important for substrate binding, but do not determine substrate specificity. With GGPP as a substrate, UPPS still...
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Biochem J (2003) 372 (1): 137–143.
Published: 15 May 2003
... correspondence should be addressed (e-mail tozser@indi.biochem.dote.hu ). 9 12 2002 14 2 2003 17 2 2003 17 2 2003 The Biochemical Society, London ©2003 2003 caspase-3 caspase-7 caspase-8 cleavage site oligopeptide substrate specificity Abbreviations used: AD...